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wild soybean
相关语句
  野生大豆
    INVESTIGATION AND RESEARCH OF THE WILD SOYBEAN IN HEILONGJIANG PROVINCE
    黑龙江省野生大豆的考察和研究
短句来源
    Isolation of Total mRNA from the Immature Beijing Wild Soybean Seeds and Molecular Cloning of Its Complementary DNA
    野生大豆未成熟种子总mRNA的分离及其cDNA的分子克隆
短句来源
    The Construction of Genomic Library of Wild Soybean
    野生大豆基因文库的构建
短句来源
    Investigaton on Embryology of Wild Soybean
    野生大豆胚胎学研究
短句来源
    The Relationship Between the Ureide Contents and Nodule Nitrogen Fixation Activities in Wild Soybean (Glycine soja) Lines
    野生大豆(Glycine soja)酰脲含量与根瘤固氮活力的关系
短句来源
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  野大豆
    Wild soybean seedlings were treated with 0, 50, 100 and 200 mmol/L NaCl , then the Na+ , Cl- and K+ content of shoots and roots were measured and compared.
    用不同浓度的NaCl溶液处理野大豆幼苗后,测定并比较根及地上部的Na+、Cl-、K+含量.
短句来源
    The effect of concentrated sulfuric acid on germination of wild soybean in Yellow River Delta Area
    浓硫酸处理对黄河三角洲野大豆发芽效果的影响
短句来源
    The chromosomesof wild soybean of Glycine and Soja subgenus wereanalyzed to study theirgenetic diversity on thechromosomal level.
    对我国大豆属Glycine亚属2种多年生野生大豆进行了细胞染色体观察,结果表明:多年生野生大豆———G. tomentella(短绒野大豆、多毛豆)和G.
短句来源
    Before being planted artificially,the hard seed of wild soybean in Yellow River Delta Area must be treated to improve the germination rate.
    在对黄河三角洲野大豆Glycine soja人工栽培的研究中,先进行了野大豆种子播种前处理方法的研究,破除其种皮硬实,提高发芽率。
短句来源
  “wild soybean”译为未确定词的双语例句
    and wild soybean ( dycine soja (L.)
    和野大豆(Glycine soja(L.)
短句来源
    For salt-sensitive wild soybean(N23232),the O -. 2 generation rate and H 2O 2 content increased, product of lipid peroxidation(MDA) and electrical conductivity increased in the leaves under 130 mmol/L NaCl stress. There were some significantly positive correlation among the Na +?
    耐盐性弱的N2 32 32叶片O- 2 产生速率和H2 O2 含量上升 ,膜脂过氧化产物MDA和电导率迅速上升 ,其叶片中Na+ 、Cl- 含量变化与O- 2 、H2 O2 、MDA和电导率增加呈极显著或显著正相关 ,而O- 2 产生速率和H2 O2 含量上升与MDA和电导率变化呈极显著正相关。
短句来源
    Morphology of Pollen of Perennial Wild Soybean
    大豆属Glycine亚属植物花粉形态研究
短句来源
    Effect of NaCl stress on the content of inorganic ions in wild soybean
    盐胁迫下野大豆无机离子分布的研究
短句来源
    Compared to the control, 22 of ESTs expressed under 20% PEG treated in wild soybean were sequenced.
    进行反向Northern Blot,鉴定出52个阳性克隆,选取差异表达明显的22个克隆测序得到19个有效的EST序列。
短句来源
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  wild soybean
Chimeric RNA3s from between CMV-SC and CMV-Y, and chimeric RNA3s from between CMV-SC and CMV-SD, were made and inoculated onto wild soybean Iwate and soybean cv.
      
The efficiency of somatic embryo germination was as high as 77% from semi-wild soybean and 60-64% from cultivated soybeans, showing that the plant regeneration system developed in this study was efficient and practical.
      
Rhodamine conjugated LPS from both strains ofRhizobium japonicum did not exhibit specific binding to wild soybean seedling roots.
      
Lack of specific binding of labeledRhizobium japonicum lipopolysaccharides to wild soybean (Glycine soja) roots
      
NaCl induced the increase of CEF1 more greatly in wild soybean Glycine cyrtoloba (cv.
      
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This paper deals mainly with the results of observation.investigation and identification on the specimens and theirresearch deta of some wild species of Genus Glycine from Li-aoning Province. The specimens mentioned above had extensi-vely been collected and cultured by Tieling Institute of Agri-cultural Research in recent years. New yarieties of wild soybean, i. e. "Glycine soja Sieb. et Zucc. var. albiflora P. Y. Fuet Y. A. Chen" and its new variant form "G. soja Sieb. etZucc. var. albiflora P. Y. Fu...

This paper deals mainly with the results of observation.investigation and identification on the specimens and theirresearch deta of some wild species of Genus Glycine from Li-aoning Province. The specimens mentioned above had extensi-vely been collected and cultured by Tieling Institute of Agri-cultural Research in recent years. New yarieties of wild soybean, i. e. "Glycine soja Sieb. et Zucc. var. albiflora P. Y. Fuet Y. A. Chen" and its new variant form "G. soja Sieb. etZucc. var. albiflora P. Y. Fu et Y. A. Chen f. angustifoliaP. Y. Fu et Y. A. Chen", which have a certain position innature and are more stable in cultural experiments, are publi-shed. The new Chinese name of "G. gracilis Skv. var. nigraSkv." is proposed and the meaning of its correct name--theextent of its characterization is revised.In the present paper, the legitimate and correct name, sy-nonym, its brief description, habitat and distribution of eachtaxon are recorded in order to identify conveniently. At thesame time, a key to t ication of wild species of Genuslycine from Liaoning Province is provided for application.,However, a further study of the problems on the classificationof variant forms of their seeds is considered to be needed.

本文主要叙述了对铁岭农科所几年来广泛调查采集和栽培的辽宁野生的大豆属植物的情况和标本等进行观察和研究鉴定的结果。发表了在自然界占有一定位置、栽培试验比较稳定的野大豆的新的变种“白花野大豆”Glycine soja Sieb.et Zucc.var.albiflora P.Y.Fu et Y.A.Chen“和新变型“狭叶白花野大豆”Glycine soja Sieb.et Zucc.var.albiflora P.Y.Fu et Y.A.Chen f.angustifolia P.Y.Fu.etY.A.Chen。提出“白花宽叶蔓豆”的新中名,并对其拉丁学名正确名称的含义——特征范围作出修正。为了便于鉴别,记述了每个种型的拉丁学名、有关异名、简要特征及生境、产地和分布,同时提出了辽宁省野生大豆属植物分类检索表以利应用。对于其种子的变异类型的分类问题认为是需要另行研究解决的。

Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%. The mRNA sample showed enough translation activity when assayed in the rabbit reticulocyte cell-frees ystem. Electrophoresis on agrose gel containing methylmercuric hydroxide indicated that the molecular size of mRNA was between 10S and 30S, of which two main components were 25S and 18S superabundant...

Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%. The mRNA sample showed enough translation activity when assayed in the rabbit reticulocyte cell-frees ystem. Electrophoresis on agrose gel containing methylmercuric hydroxide indicated that the molecular size of mRNA was between 10S and 30S, of which two main components were 25S and 18S superabundant mRNA coding for soybean storage proteins.

以SDS—苯酚法从北京野生大豆未成熟种子中制备总核糖核酸(RNA),经Oligo(dT)—纤维素柱亲和层析获得总信使核糖核酸(mRNA),约占总RNA的5.3%。经兔网织红细胞体系测定,mRNA样品表现出相当的翻译活力。氢氧化甲基汞凝胶电泳结果表明,总mRNA的分子量范围为10S~30S,其中两个主要组分为25S和18S的超丰富贮藏蛋白mRNA。

Total RNA of the immature Beijing wild soybean seeds was isolated by Lid precipitation method.Total mRNA was purified by oligo(dT)-cellulose affinity chromatography.The mRNA thus prepared showed enough translation activity when assayed in the rabbit reticulo-cyte cell-free system in vitro.Using the total mRNA as template and oligo(dT)12-18 as primer,the first strand and second strand cDNA were synthesized respectively by AMV reverse tran-scriptase and RNase H-DNA polymerase I.The length of the double stranded...

Total RNA of the immature Beijing wild soybean seeds was isolated by Lid precipitation method.Total mRNA was purified by oligo(dT)-cellulose affinity chromatography.The mRNA thus prepared showed enough translation activity when assayed in the rabbit reticulo-cyte cell-free system in vitro.Using the total mRNA as template and oligo(dT)12-18 as primer,the first strand and second strand cDNA were synthesized respectively by AMV reverse tran-scriptase and RNase H-DNA polymerase I.The length of the double stranded cDNA synthesized was about 200-5000 bp.The ds-cDNA with perfect plum ends was inserted into the Smal site of pUC19 by blunt end ligation.The resultant recombinant molecules were used to transform E.coli strain JM 107,and more than 800 white clones were obtained.Rapid electrophoresis and EcoRI-HindIII digestion analysis showed that most of the recombinant plasmids from the white clones contained insertions with various lengths and 3 out of 4 tested were about 1700,2600 and 1400 bp in length respectively.

用氯化锂沉淀法从野生大豆(G.soja)未成熟种子中制备总RNA,经oligo(dT)-纤维素柱亲和层析,获得总mRNA,在兔网织红细胞体系中表现出一定翻译活性。以总mRNA为模板,oligo(dT)_(12)(?)为引物,反转录酶催化合成第一链cDNA,RNase H-DNA聚合酶Ⅰ协同合成第二链cDNA。双链cDNA的长度大约为200—5000 bp,且不存在发夹结构。将双链cDNA修补后钝端连接到pUC 19质粒的Sma 1位点,转化E.coli JM107,获得800多个白色重组子克隆。快速电泳检测及酶切分析表明,多数重组子带有插入片段,其中3个重组子的插入片段长度大致为1700 bp、2600 bp和1400 bp。

 
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