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osteogenesis ability
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  成骨能力
     Conclusion The osteogenesis ability of TGF-β/SSO is superior to that of TGF-β/HAP,which indicates that the compound material of TGF-β/SSO can be applied to cranial bone defects of children.
     结论:TGF-β/SSO成骨能力大于TGF-β/HAP,说明TGF-β与骨松质的复合材料可以作为小儿颅骨大面积缺损的修复材料。
短句来源
     DATA SOURCES: A computer-based online search of Pubmed and Ovid databases was undertakne to identify the articles about the biological characteristics and osteogenesis ability of fibroblasts published in English between January 1990 and December 2006 with the key words of "fibroblasts, osteogenesis".
     资料来源:应用计算机检索Pubmed,Ovid数据库1990-01/2006-12有关成纤维细胞生物学特性及其成骨能力的文章,检索词“fibroblasts,osteogenesis”,限定文章语言种类为English。
短句来源
     The osteogenesis ability of BMSCs on the DBM was determined by assessment of the alkaline phosphatase(ALP) activity and osteocalcin(OCN)content.
     通过测定碱性磷酸酶(ALP)活性和骨钙素(OCN)含量观察细胞在DBM上的成骨能力
短句来源
     CONCLUSION: Gene transfer of Cbfa1 to fibroblast NIH3T3 can alter the conversion of the phenotype of osteogenesis, and then the quantity of osteoblast increased and made the increase of osteogenesis ability possible.
     结论:核心结合因子α1基因转移NIH3T3成纤维细胞改变了成纤维细胞向成骨表型转化,增加了成骨细胞数量,使成骨能力增强成为可能。
短句来源
     Conclusions Bio derived bone could be applied as the scaffold materials of bone tissue engineering, and CFDB has good osteogenesis ability.
     结论 生物衍生骨支架材料可作为骨组织工程支架材料 ,成骨能力以CFDB为最好
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  成骨作用
     It is known that extracorporeal shock wave (SW) may promote healing of fracture. A previous study reported that SW promoted human bone marrow stromal cells (hMSCs) towards osteoblasts in vitro. To study the osteogenesis ability of hMSCs treated by shock wave in porous hydroxyapatite (HA) in vivo, primary hMSCs of SW group and control group were cultured in the porous HA for 2 weeks and then implanted into subcutaneous sites of nude mouse.
     为了研究冲击波(SW)诱导人骨髓基质细胞(hMSCs)在动物体内成骨作用,根据前期工作结果,应用适宜能量冲击波(10kV,500次)处理体外培养的hMSCs,将SW组和对照组hMSCs与羟基磷灰石(HA)载体复合后体外培养2周,应用扫描电镜(SEM)检测细胞在载体表面的生长情况.
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  “osteogenesis ability”译为未确定词的双语例句
     Objective To study the bone tissue osteogenesis ability of bone marrow stem cells (BMSCs) in vitro in order to offer the ideal seed cells for tissue engineering.
     目的 探索兔骨髓基质干细胞(bone marrow stem cells BMSCs)在体外诱导为成骨细胞的成骨特性,为骨组织工程提供理想的种子细胞。
短句来源
     group B had a better osteogenesis ability than group A.
     B组材料修复能力较C组差,但B组和C组均优于A组。
短句来源
     ②Basic and clinical study in the fibroblasts and osteogenesis ability.
     ②有关成纤维细胞与成骨的基础与临床研究。
短句来源
     Objective To study the bone tissue osteogenesis ability of bone marrow stromal cells (BMSCs) in vitro and in vivo.
     目的研究骨髓基质干细胞(Bonemarrowstromalcells,BMSCs)在体外培养、诱导后的成骨活性,探讨BMSCs作为骨组织工程种子细胞的可能性和可行性。
短句来源
     The segmental bone defects were treated with implantation of CPC or PBAB.The osteogenesis ability of PBAB was evaluated by X ray,histomorphological observation and biomechanical measurement after operation.
     术后通过X射线检查、组织形态学观察、生物力学检测等手段综合评价PBAB修复骨缺损的能力。
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Objective To study the ectopic osteogenesis of bio derived bone composited with osteoblasts after implantation in vivo and explore their possibility applied as the scaffold material of bone tissue engineering. Methods Compound fully deproteinized bone (CFDB), partially deproteinized bone (PDPB) and partially decalcified bone (PDCB) treated physically and chemically were composited with human embryonic periosteal osteoblasts, cultured for one week, then,were implanted into nude mice. The osteogenesis...

Objective To study the ectopic osteogenesis of bio derived bone composited with osteoblasts after implantation in vivo and explore their possibility applied as the scaffold material of bone tissue engineering. Methods Compound fully deproteinized bone (CFDB), partially deproteinized bone (PDPB) and partially decalcified bone (PDCB) treated physically and chemically were composited with human embryonic periosteal osteoblasts, cultured for one week, then,were implanted into nude mice. The osteogenesis of materials with cells were studied through gross observation , alkaline phosphatase activity assay, hematoxylin and eosin staining and quantitative analysis. Results CFDB ,PDPB,PDCB composited with osteoblasts could form cartilage tissue and bone formation in vivo, and more new bone tissue formed gradually as the time after implantation went by. The contents of ossification were CFDB>PDPB>PDCB. However, in the control group there was no bone formation. Conclusions Bio derived bone could be applied as the scaffold materials of bone tissue engineering, and CFDB has good osteogenesis ability.

目的 探讨生物衍生骨支架材料复合成骨细胞体内植入的成骨作用 ,了解其用于骨组织工程支架材料的可行性。 方法 将经物理、化学方法处理制得的复合型完全脱蛋白骨(CFDB)、部分脱蛋白骨 (PDPB)、部分脱钙骨 (PDCB) 3种生物衍生骨支架材料分别与人胚骨膜成骨细胞体外复合培养 1周后 ,植入裸鼠背部肌肉内作为实验组 (30只 ) ,以单纯材料植入为对照组 (30只 ) ,经大体观察、碱性磷酸酶活性测定、常规组织学检查及植入物成骨的定量判断 ,观测细胞 -材料复合物的成骨作用。 结果 组织学检查证明 ,实验组 3种材料皆有成骨作用 ,并随植入时间的延长 ,新骨形成增多 ;成骨含量分析表明 ,术后 4周和 8周 ,CFDB组组织学评分分别为 2 .90± 0 .5 7和 4.6 0± 0 .70 ,显著优于其他实验组。对照组无成骨现象。 结论 生物衍生骨支架材料可作为骨组织工程支架材料 ,成骨能力以CFDB为最好

Objective To study the bone tissue osteogenesis ability of bone marrow stromal cells (BMSCs) in vitro and in vivo. Methods The BMSCs were separated by gradient centrifugation on Percoll, culture-expanded and induced with a conditional medium. The cells were examined by transmission electron microscopy and histochemistry or immunochemistry stains technique including AKP,Cbfa1,OCN, OPN, BSP and collagen type Ⅰ. The mRNA of collagen type I in cultured cell was also examined by in situ hybridization. Results...

Objective To study the bone tissue osteogenesis ability of bone marrow stromal cells (BMSCs) in vitro and in vivo. Methods The BMSCs were separated by gradient centrifugation on Percoll, culture-expanded and induced with a conditional medium. The cells were examined by transmission electron microscopy and histochemistry or immunochemistry stains technique including AKP,Cbfa1,OCN, OPN, BSP and collagen type Ⅰ. The mRNA of collagen type I in cultured cell was also examined by in situ hybridization. Results Histochemical studies showed positive stain of AKP,Cbfa1, OCN, OPN,BSP and collagen type Ⅰ,and in situ hybridization also showed the positive express of mRNA of collagen type I. The excretion of collagen and deposition of calcium salt were observed under transmission electron microscopy. Conclusion BMSCs cultured in conditional medium shows osteoblast-like functions in vitro, which provides an ideal seed cells for bone tissue engineering.

目的研究骨髓基质干细胞(Bonemarrowstromalcells,BMSCs)在体外培养、诱导后的成骨活性,探讨BMSCs作为骨组织工程种子细胞的可能性和可行性。方法抽取成年犬骨髓,用梯度离心法获取单核细胞,经条件培养液体外诱导培养后,进行组织化学、免疫组化、原位杂交检测,并在透射电镜下观察其成骨活性。结果第1代细胞Alkalinephosphatase(AKP)染色、Core-bindingfactoralphasubunit1(Osf2/Cbfa1)、Osteopotin(OPN)、Ⅰ型胶原的免疫细胞化学检测和I型胶原的原位杂交结果开始呈现阳性,第3代细胞BoneSailoplain(BSP)、Osteocalcin(OCN)免疫细胞化学染色开始呈现阳性,透射电镜显示细胞外有胶原分泌和钙盐沉积。结论BMSCs不仅能大量扩增,且在一定条件下可向成骨细胞分化,是骨组织工程理想的种子细胞。

Objective: To evaluate the osteogenesis and immunoreaction of the 3rd generation osteoblasts processed by cryopreservation in SD rats. Methods: Osteoblasts were derived from calvaria of newborn SD rats,then were transplanted into the decalcified bone (DB) of porcine after the cells were passaged for 3rd generations. Some 3rd generation cells were frozen by -196℃ hypothermia, then implanted into the dorsum muscle of SD rats as were done to another part of 3rd generation cells and the pure DB( n =24). Four weeks...

Objective: To evaluate the osteogenesis and immunoreaction of the 3rd generation osteoblasts processed by cryopreservation in SD rats. Methods: Osteoblasts were derived from calvaria of newborn SD rats,then were transplanted into the decalcified bone (DB) of porcine after the cells were passaged for 3rd generations. Some 3rd generation cells were frozen by -196℃ hypothermia, then implanted into the dorsum muscle of SD rats as were done to another part of 3rd generation cells and the pure DB( n =24). Four weeks later, samples were harvested to evaluate the osteogenesis ability and immunoreactions caused by frozen cells with histology, ALP, immunoreaction and so on. Results: The bone matrix produced by frozen cells was larger in number and more mature than that of the control ones. The immune cells adhering to frozen osteoblast complex were apparently less than to control complex. Conclusion: The allogenic osteoblast antigen becomes weaker after frozen by hypothermia, but its osteogenesis still remains.

目的 :研究同种异体成骨细胞经超低温冻存后在体内的成骨能力及其免疫反应。 方法 :体外分离培养 SD大鼠颅骨成骨细胞 ,提纯并传代至第 3代时一部分作为对照组用 ;另一部分采用 - 196℃超低温冻存 ,2 4 h后复苏。将该两种第 3代细胞分别与脱细胞并去抗原性的猪脱钙型松质骨 (decalcified bone,DB)复合 ,将两种复合体和单纯 DB分别植入 SD大鼠背部肌袋内 ,各2 4只 ,4周后取标本 ,应用组织学、碱性磷酸酶测定、体视学及免疫学测定 ,观察成骨现象及免疫反应。 结果 :经超低温冻存的同种异体成骨细胞异体异位植入后 ,成骨量、成熟度明显优于未冻存成骨细胞 ;引起的免疫反应亦低于未冻存细胞。 结论 :同种异体成骨细胞经超低温冻存、复苏后 ,抗原性明显降低 ,未引起强烈的免疫排斥反应 ,仍具有成骨能力

 
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