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gm maize
相关语句
  转基因玉米
     PCR on Detection and Identification of GM Maize MON810
     PCR对转基因玉米MON810的鉴定检测
短句来源
     One primers was de-signed based on CaMV35S promoter with a amplicon of195bp to detect MON810GM maize.
     同时扩增CaMV35S启动子基因195bp片段,可以对MON810转基因玉米进行筛选检测;
短句来源
     The specific primers of MON810were designed as Maize genome /CaMV35S gene with a amplicon of170bp and HSP/CryIA(b)gene with a amplicon of194bp to identify the kind of GM maize MON810.PCR amplification condition was one cycle of94℃for2min,then35cycle of(94℃for40sec,55℃for60sec,72℃for60sec),and finally one cycle of72℃for5min.
     此外,根据MON810品种设计的特异性鉴定检测引物,可扩增Maizegenome/CaMV35S基因170bp片段和HSP/CryIA(b)基因194bp片段,具有很强的品种特异性,达到了对MON810转基因玉米的品种鉴定目的。 PCR反应循环参数是94℃2min;
短句来源
     Theresults demonstrated this method is useful for identifying the event-specific GM maize Mon810. Some samples were tested notonly the GM maize Mon810 but also another GMO components.
     某些检测样品不仅检出转基因玉米Mon810成分,还同时检出其它转基因玉米品系或其它转基因品种。 本研究实验建立的转基因玉米Mon810品系鉴定检测方法,即可以用于加工产品中转基因成分的定量检测(检测低限为0.1%),也可以用于定性检测,或作为常规PCR定性检测后的确证实验方法。
短句来源
     Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays. The RTF PCR method was established to detect and identify GM maize lines.
     根据转基因玉米T14/T25转入的外源基因质粒图谱,设计转基因引物和探针进行PCR和实时荧光PCR检测,建立了转基因玉米品系鉴定的实时荧光PCR方法。
短句来源
  gm玉米
     Primers specific for inserted genes in the Event 176 GM maize (Novartis company), Bt11 (Novartis company), Mon810 (Monsanto company), T14/T25 (AgrEvo company), CBH-351 (AgrEvo company) and GA21 (Monsanto company) were used to conduct the PCR assays. PCR method was established to detect and identify lines of GM maize.
     针对Mon810 (Monsanto公司 )、Bt11(Novartis公司 )、Event176 (Novartis公司 )、CBH 35 1(AgrEvo公司 )、T14/T2 5Liberty (AgrEvo公司 )及GA2 1(Monsanto公司 )GM 玉米转入的外源基因质粒图谱 ,设计具有品系特异性的引物进行PCR检测 ,建立了GM 玉米品系鉴定检测的方法。
短句来源
  “gm maize”译为未确定词的双语例句
     A Change of N and K Contents in GM Maize Transmitted with Soybean DNA
     转大豆DNA玉米的氮钾含量测定
短句来源
     Based on multiplex PCR method,this research construct a single-tube amplification system including 3 different fragments of transgenes,regulating elements and endogenous genes by screening of fragment and adjusting of primer proportion on 3 different crops of Roundup ready soybean,Bt 176 GM maize and Cecropin D capsicum.
     本研究以国际市场上转基因食品的主流产品转抗除草剂大豆 (RR大豆 )、Bt176玉米及我国自行研发的抗菌肽辣椒为材料 ,以多重PCR方法为基础 ,通过基因片段筛选及引物比例调整 ,进行了转基因食品的调控元件、外源结构基因及物种内源特性基因三种片段的单管同时扩增。
短句来源
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  gm maize
A total of 24 laboratories participated in the study and each laboratory received 18 DNA blind samples prepared from GMO certified reference materials (0.1 % and 0.5 % NK601 maize) and from a non-GM maize flour.
      
The first genetically modified (GM) maize hybrids were registered in the Commercial Variety Register in Spain on 26 March 1998.
      
However, the question of adequate isolation distances between GM and non-GM maize is still subject of controversy both amongst scientists and regulators.
      
Maize is one of the main crops worldwide and an increasing number of genetically modified (GM) maize varieties are cultivated and commercialized in many countries in parallel to conventional crops.
      
There lease of viable pollen from GM maize can be controlled by growing mixtures of cytoplasmic male-sterile plants and male-fertile non-transformed pollinator plants.
      
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Nucleotide\|based amplification method is an important system for the identification of genomic modified foods (GMF). Roundup Ready Soybeans (Monsanto company), Bt 176 GM maize (Novartis/Ciba\|Geigy company) and Cecropin D capsicum was used as material to search for the feasibility of investigating the safety of GMF by PCR method. Primers specific for inserted genes and crop endogenous genes in Roundup Ready Soybeans, Bt 176 maize and Cecropin D capsicum were applied. The discrimination system for...

Nucleotide\|based amplification method is an important system for the identification of genomic modified foods (GMF). Roundup Ready Soybeans (Monsanto company), Bt 176 GM maize (Novartis/Ciba\|Geigy company) and Cecropin D capsicum was used as material to search for the feasibility of investigating the safety of GMF by PCR method. Primers specific for inserted genes and crop endogenous genes in Roundup Ready Soybeans, Bt 176 maize and Cecropin D capsicum were applied. The discrimination system for GM soybeans, GM maize and GM capsicum from the counterpart of non\|GM products and the detection system for correlating marker genes and transgenes are established. The method was easy and fast, and the corresponding results fixed the standard or declared data.

以国际市场上转基因食品的主流产品转基因大豆及玉米和我国生产的转基因辣椒为材料 ,以PCR方法为基础 ,研究适用于转基因食品安全性检验的核酸检测技术。针对抗除草剂 (孟山都公司 )GM 大豆 ,转Bt 176玉米 (Novartis Ciba Geigy公司 )及转抗菌肽辣椒 (华农 )产品的插入基因及调控序列设计不同引物进行PCR检测。建立了转基因大豆、玉米、辣椒的鉴别和相关标记基因、目的基因检测的技术 ,该方法简便快速 ,检测结果与标准及申报材料相符。

Study the method of quantitative/identified detection of genetically modified (GM) maize Mon810 components infoods and in feeds by real-time PCR. The primer and probe set overlapping the junction was designed and used for assay. Theresults demonstrated this method is useful for identifying the event-specific GM maize Mon810. Some samples were tested notonly the GM maize Mon810 but also another GMO components.

采用实时荧光PCR技术,建立了定量(性)鉴定检测加工产品中转基因玉米Mon810成分的方法。实验设计的可以扩增玉米自身基因和外源基因边界序列的引物和探针具有品种和品系特异性,特异性地检测出食品、饲料等加工产品中转基因玉米Mon810成分。某些检测样品不仅检出转基因玉米Mon810成分,还同时检出其它转基因玉米品系或其它转基因品种。本研究实验建立的转基因玉米Mon810品系鉴定检测方法,即可以用于加工产品中转基因成分的定量检测(检测低限为0.1%),也可以用于定性检测,或作为常规PCR定性检测后的确证实验方法。

To detect and identify six lines of genetically modified (GM) maize, polymerase chain reaction (PCR) assays were performed in this study. Primers specific for inserted genes in the Event 176 GM maize (Novartis company), Bt11 (Novartis company), Mon810 (Monsanto company), T14/T25 (AgrEvo company), CBH-351 (AgrEvo company) and GA21 (Monsanto company) were used to conduct the PCR assays. PCR method was established to detect and identify lines of GM maize.

以PCR方法鉴定检测六种商业化种植的基因改良玉米 (geneticallymodifiedmaize,简称GM 玉米 )。针对Mon810 (Monsanto公司 )、Bt11(Novartis公司 )、Event176 (Novartis公司 )、CBH 35 1(AgrEvo公司 )、T14/T2 5Liberty (AgrEvo公司 )及GA2 1(Monsanto公司 )GM 玉米转入的外源基因质粒图谱 ,设计具有品系特异性的引物进行PCR检测 ,建立了GM 玉米品系鉴定检测的方法。

 
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