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gm maize
相关语句
  转基因玉米
    PCR on Detection and Identification of GM Maize MON810
    PCR对转基因玉米MON810的鉴定检测
短句来源
    One primers was de-signed based on CaMV35S promoter with a amplicon of195bp to detect MON810GM maize.
    同时扩增CaMV35S启动子基因195bp片段,可以对MON810转基因玉米进行筛选检测;
短句来源
    The specific primers of MON810were designed as Maize genome /CaMV35S gene with a amplicon of170bp and HSP/CryIA(b)gene with a amplicon of194bp to identify the kind of GM maize MON810.PCR amplification condition was one cycle of94℃for2min,then35cycle of(94℃for40sec,55℃for60sec,72℃for60sec),and finally one cycle of72℃for5min.
    此外,根据MON810品种设计的特异性鉴定检测引物,可扩增Maizegenome/CaMV35S基因170bp片段和HSP/CryIA(b)基因194bp片段,具有很强的品种特异性,达到了对MON810转基因玉米的品种鉴定目的。 PCR反应循环参数是94℃2min;
短句来源
    Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays. The RTF PCR method was established to detect and identify GM maize lines.
    根据转基因玉米T14/T25转入的外源基因质粒图谱,设计转基因引物和探针进行PCR和实时荧光PCR检测,建立了转基因玉米品系鉴定的实时荧光PCR方法。
短句来源
  转基因玉米
    PCR on Detection and Identification of GM Maize MON810
    PCR对转基因玉米MON810的鉴定检测
短句来源
    One primers was de-signed based on CaMV35S promoter with a amplicon of195bp to detect MON810GM maize.
    同时扩增CaMV35S启动子基因195bp片段,可以对MON810转基因玉米进行筛选检测;
短句来源
    The specific primers of MON810were designed as Maize genome /CaMV35S gene with a amplicon of170bp and HSP/CryIA(b)gene with a amplicon of194bp to identify the kind of GM maize MON810.PCR amplification condition was one cycle of94℃for2min,then35cycle of(94℃for40sec,55℃for60sec,72℃for60sec),and finally one cycle of72℃for5min.
    此外,根据MON810品种设计的特异性鉴定检测引物,可扩增Maizegenome/CaMV35S基因170bp片段和HSP/CryIA(b)基因194bp片段,具有很强的品种特异性,达到了对MON810转基因玉米的品种鉴定目的。 PCR反应循环参数是94℃2min;
短句来源
    Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays. The RTF PCR method was established to detect and identify GM maize lines.
    根据转基因玉米T14/T25转入的外源基因质粒图谱,设计转基因引物和探针进行PCR和实时荧光PCR检测,建立了转基因玉米品系鉴定的实时荧光PCR方法。
短句来源
  gm玉米
    Primers specific for inserted genes in the Event 176 GM maize (Novartis company), Bt11 (Novartis company), Mon810 (Monsanto company), T14/T25 (AgrEvo company), CBH-351 (AgrEvo company) and GA21 (Monsanto company) were used to conduct the PCR assays. PCR method was established to detect and identify lines of GM maize.
    针对Mon810 (Monsanto公司 )、Bt11(Novartis公司 )、Event176 (Novartis公司 )、CBH 35 1(AgrEvo公司 )、T14/T2 5Liberty (AgrEvo公司 )及GA2 1(Monsanto公司 )GM 玉米转入的外源基因质粒图谱 ,设计具有品系特异性的引物进行PCR检测 ,建立了GM 玉米品系鉴定检测的方法。
短句来源
  “gm maize”译为未确定词的双语例句
    A Change of N and K Contents in GM Maize Transmitted with Soybean DNA
    转大豆DNA玉米的氮钾含量测定
短句来源
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  gm maize
A total of 24 laboratories participated in the study and each laboratory received 18 DNA blind samples prepared from GMO certified reference materials (0.1 % and 0.5 % NK601 maize) and from a non-GM maize flour.
      
The first genetically modified (GM) maize hybrids were registered in the Commercial Variety Register in Spain on 26 March 1998.
      
However, the question of adequate isolation distances between GM and non-GM maize is still subject of controversy both amongst scientists and regulators.
      
Maize is one of the main crops worldwide and an increasing number of genetically modified (GM) maize varieties are cultivated and commercialized in many countries in parallel to conventional crops.
      
There lease of viable pollen from GM maize can be controlled by growing mixtures of cytoplasmic male-sterile plants and male-fertile non-transformed pollinator plants.
      
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To detect and identify six lines of genetically modified (GM) maize, polymerase chain reaction (PCR) assays were performed in this study. Primers specific for inserted genes in the Event 176 GM maize (Novartis company), Bt11 (Novartis company), Mon810 (Monsanto company), T14/T25 (AgrEvo company), CBH-351 (AgrEvo company) and GA21 (Monsanto company) were used to conduct the PCR assays. PCR method was established to detect and identify lines of GM maize.

以PCR方法鉴定检测六种商业化种植的基因改良玉米 (geneticallymodifiedmaize,简称GM 玉米 )。针对Mon810 (Monsanto公司 )、Bt11(Novartis公司 )、Event176 (Novartis公司 )、CBH 35 1(AgrEvo公司 )、T14/T2 5Liberty (AgrEvo公司 )及GA2 1(Monsanto公司 )GM 玉米转入的外源基因质粒图谱 ,设计具有品系特异性的引物进行PCR检测 ,建立了GM 玉米品系鉴定检测的方法。

A quantitative/identified method was developed for detecting genetically modified (GM) maize Bt11 components in foods and in feeds by real time PCR. The event specific primer and probe set overlapping the junction was designed and used for assay. The effect of heat treatment on the detection of the genes by quantitative PCR methods was investigated. The Results revealed that the measure of inserted genes was affected by temperature and different time intervals of heat treatment. For further understanding...

A quantitative/identified method was developed for detecting genetically modified (GM) maize Bt11 components in foods and in feeds by real time PCR. The event specific primer and probe set overlapping the junction was designed and used for assay. The effect of heat treatment on the detection of the genes by quantitative PCR methods was investigated. The Results revealed that the measure of inserted genes was affected by temperature and different time intervals of heat treatment. For further understanding of whether the commercially available processed maize materials mixed with GM maize, the samples of foods and feeds were collected from market and detected by the real time PCR methods developed in this study. Results showed that both foods and feeds could be detected GM maize Bt11 components.

实验在玉米自身基因和外源基因的边界序列之间设计了具有品种和品系特异性的引物和探针 ,并以实时荧光PCR技术 ,建立了加工产品中转基因玉米Bt1 1成分品系鉴定检测和定量检测的方法。实验对加热条件和时间对检测转基因成分的影响作了探讨 ,并检测了部分市售食品和饲料。检测结果发现 ,加热时间温度越高、时间越长 ,对转基因成分定量检测的影响越大 ;在所检测的样品中可以检测出转基因玉米Bt1 1成分 ,有些样品还同时检出其他转基因成分。本研究实验建立的方法 ,可以用于加工产品中转基因成分的定量检测 ,也可以用于定性检测 ,或作为常规PCR定性检测后的确证实验方法。

PCR method was established to detect and identify GM maize MON810.A set of commonly used primers of IVR sequence of maize were designed as endogenuous reference gene with a amplicon of226bp,and check on the quality of template extraction from maize,and avoid the result of false negative.One primers was de-signed based on CaMV35S promoter with a amplicon of195bp to detect MON810GM maize.The specific primers of MON810were designed as Maize genome /CaMV35S gene with a amplicon of170bp...

PCR method was established to detect and identify GM maize MON810.A set of commonly used primers of IVR sequence of maize were designed as endogenuous reference gene with a amplicon of226bp,and check on the quality of template extraction from maize,and avoid the result of false negative.One primers was de-signed based on CaMV35S promoter with a amplicon of195bp to detect MON810GM maize.The specific primers of MON810were designed as Maize genome /CaMV35S gene with a amplicon of170bp and HSP/CryIA(b)gene with a amplicon of194bp to identify the kind of GM maize MON810.PCR amplification condition was one cycle of94℃for2min,then35cycle of(94℃for40sec,55℃for60sec,72℃for60sec),and finally one cycle of72℃for5min.

本研究成功建立了商业化种植的转基因玉米MON810的筛选检测和品种鉴定的PCR方法。该方法根据玉米自身IVR基因作为内源特异参照基因扩增226bp片段,检查模板DNA提取的质量,避免了假阴性结果;同时扩增CaMV35S启动子基因195bp片段,可以对MON810转基因玉米进行筛选检测;此外,根据MON810品种设计的特异性鉴定检测引物,可扩增Maizegenome/CaMV35S基因170bp片段和HSP/CryIA(b)基因194bp片段,具有很强的品种特异性,达到了对MON810转基因玉米的品种鉴定目的。PCR反应循环参数是94℃2min;94℃40sec,55℃60sec,72℃60sec,35次循环;之后72℃延伸5min。

 
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