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genetically modified maize
相关语句
  转基因玉米
     Identified and Quantitative Detection of the Genetically Modified Maize Mon810 Components in Processed Products
     实时荧光PCR定量检测加工产品中转基因玉米Mon810成分
短句来源
     Quantitative/Identified Detection of the Genetically Modified Maize Bt11 Components in Processed Products
     加工产品中转基因玉米Bt11成分实时荧光PCR定量(性)检测
短句来源
     Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method
     用实时荧光PCR方法鉴定转基因玉米T14/T25
短句来源
     Based on the antibody-antigen-enzyme antibody reaction and by using puriftied Bt1 insecticidal crystal protein asboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was estab-lished to detect express Bt1 protein in genetically modified maize.
     应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。
短句来源
  “genetically modified maize”译为未确定词的双语例句
     Method for the Identification of Six lines of Genetically Modified Maize
     商业化种植的六种基因改良玉米品系的鉴定检测方法
短句来源
     HETEROLOGOUSLY QUANTITATIVE COMPETITIVE PCR FOR THE DETECTION OF GENETICALLY MODIFIED MAIZE
     玉米转基因成分的非同源模板竞争定量PCR检测
短句来源
     Based on the reaction among antibody antigen enzyme antibody, using purified Bt1 insecticide crystal protein as standard protein and immunity antigen, a quantitative indirect enzyme linked immunosorbent assay (ELISA) method was established to detect Bt1 protein expressed in genetically modified maize.
     应用纯化的Bt1杀虫晶体蛋白质作为标准蛋白和免疫抗原 ,通过抗体 抗原 酶标抗体反应 ,建立了间接酶联免疫吸附测定法 (ELISA) ,以快速检测转基因抗虫玉米中的Bt1表达蛋白 .
短句来源
     The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.
     建立了玉米转基因成分中35S启动子非同源模板的竞争定量PCR检测系统.
短句来源
  相似匹配句对
     Genetically modified food
     谁来捅破转基因这层窗户纸
短句来源
     Detection of Genetically Modified Food
     转基因食品的检测
短句来源
     of modified P.R.
     R.
短句来源
     and maize cross.
     与玉米杂交导入一个小麦加倍单倍体 (DH)植株中。
短句来源
     The results of maize are reverse.
     玉米的结果刚好相反。
短句来源
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  genetically modified maize
The use of the Matrix isillustrated by applying it to an example of a ``novelfood,'' viz., a form of genetically modified maize.
      
Controlling the release of pollen from genetically modified maize and increasing its grain yield by growing mixtures of male-ste
      
New Analysis of a Rat Feeding Study with a Genetically Modified Maize Reveals Signs of Hepatorenal Toxicity
      
Fate of recombinant DNA and Cry1Ab protein after ingestion and dispersal of genetically modified maize in comparison to rapeseed
      
Investigations on genetically modified maize (Bt-maize) in pig nutrition: fate of feed-ingested foreign DNA in pig bodies
      
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Based on the antibody-antigen-enzyme antibody reaction and by using puriftied Bt1 insecticidal crystal protein asboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was estab-lished to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, Incomparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISAmethod might be useful for easy,rapid,reliable...

Based on the antibody-antigen-enzyme antibody reaction and by using puriftied Bt1 insecticidal crystal protein asboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was estab-lished to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, Incomparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISAmethod might be useful for easy,rapid,reliable and effective assay in large samples.

应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。

Based on the reaction among antibody antigen enzyme antibody, using purified Bt1 insecticide crystal protein as standard protein and immunity antigen, a quantitative indirect enzyme linked immunosorbent assay (ELISA) method was established to detect Bt1 protein expressed in genetically modified maize.4 import maize samples were detected by the ELISA, compared to western blotting & KIT assay methods. and results were agreeable.

应用纯化的Bt1杀虫晶体蛋白质作为标准蛋白和免疫抗原 ,通过抗体 抗原 酶标抗体反应 ,建立了间接酶联免疫吸附测定法 (ELISA) ,以快速检测转基因抗虫玉米中的Bt1表达蛋白 .用建立的ELISA法对 4种进口玉米实物样品进行了测定 ,实验结果得到了免疫印迹分析的验证 ,并与进口试剂盒方法的定量分析结果相一致

The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.The key problem of QC-PCR is the construction of internal standard.The heterologous template is constucted as follow:the E.coli genomic DNA was low stringently amplified,the selected band was purified and ligated pGEM-T Easy Vector.The competitor concentrations were adjusted by the content of the heterologous template...

The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.The key problem of QC-PCR is the construction of internal standard.The heterologous template is constucted as follow:the E.coli genomic DNA was low stringently amplified,the selected band was purified and ligated pGEM-T Easy Vector.The competitor concentrations were adjusted by the content of the heterologous template in a way that the equivalence point represented a GMO content of 1%.Therefore,the minimum detectable quantity was 1%.Then template DNA mixtures containing different proportion GMO were co-amplified with constant internal standard concentration of further determine GMO content in food samples.

建立了玉米转基因成分中35S启动子非同源模板的竞争定量PCR检测系统.竞争定量PCR的关键问题是内标物的制备.实验中非同源模板的构建是采用PCR方法对大肠杆菌基因组进行低严紧型扩增,回收预期DNA片段并将其构建到T easy载体上.通过调整非同源模板浓度使竞争物PCR产物亮度与1%GMO玉米的亮度相同,从而确定转基因玉米的检出限为1%.然后以确定的内标物浓度与不同含量的GMO玉米样品进行竞争定量PCR反应,以此进一步确定样品中GMO的含量范围.

 
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