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balance time
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  平衡时间
    The frozen-thawing sperm mobility(0.193) and recovery rate of sperm(0.250) keeping in 4 °C for 30 min were not significant different from that of 4 °C for 60 min(0.249,0.325 respectively)(P>0.05). But along with prolongation of the balance time(120~180 min),the frozen-thawing sperm mobility lowered obviously,and sperm was almost dead.
    (3)在4°C下平衡30、60、120、180 m in,结果发现,在30~60 m in之间,解冻后精子活率之间(0.261 vs 0.249),复苏率之间(0.340 vs 0.325)差异均不显著(P>0.05),但是随着平衡时间(120~180 m in)的增加,精子活率明显降低,精子几乎死亡。
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  balance time
Two principal components of winter balance time-series explain about 50% of the variability in the data.
      
Significant improvements in knee extensor isometric strength (+21%), single leg balance time (+30%), and 6?min walk distance (+6%) were observed in TRA (P>amp;lt;0.05) but not in CON.
      
CW and indicators of functional capacity (knee extensor isometric strength, single leg balance time, sit and reach, stand and reach, and 6?min walk distance) were assessed prior to and following the intervention.
      
Following the practice sessions, the balance time increased significantly in the experimental group (P>amp;lt;0.01).
      
Only flunitrazepam 4?mg produced a significant decrease in balance time.
      
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The present work studied on rabbit semen cryopreservation.According to frozen-thawing sperm mobility and recovery rate of sperm,3 formulation of extenders(1: Tris 0.25 mol/L+Sucrose 0.1 mol/L+Sodium citrate 83 mmol/L+Yelk 10%+DMSO 15%;2:Formulation 1+Glucose 0.47 mmol/L;3: Tris 0.29 mol/L+Glucose 0.71 mmol/L+Sodium citrate 67 mmol/L+Yelk 20% +Glycerin 4%),2 types of cryoprotectants(glycerin 4%,DMSO 15%) and difference balance times(30,60,120,180 min) were studied respectively.The results were as followed:Among...

The present work studied on rabbit semen cryopreservation.According to frozen-thawing sperm mobility and recovery rate of sperm,3 formulation of extenders(1: Tris 0.25 mol/L+Sucrose 0.1 mol/L+Sodium citrate 83 mmol/L+Yelk 10%+DMSO 15%;2:Formulation 1+Glucose 0.47 mmol/L;3: Tris 0.29 mol/L+Glucose 0.71 mmol/L+Sodium citrate 67 mmol/L+Yelk 20% +Glycerin 4%),2 types of cryoprotectants(glycerin 4%,DMSO 15%) and difference balance times(30,60,120,180 min) were studied respectively.The results were as followed:Among the 3 formulation of extenders,formulation 2 is the best one,which frozen-thawing sperm mobility and recovery rate of sperm are higher than those of formulation 1 and 3(0.153 vs 0.103,0.109;0.205 vs 0.137,0.147,P<0.01).When using 4% glycerin as a cryoprotectant in extender 2,frozen-thawing sperm mobility(0.193),and recovery rate of sperm(0.250) were extremely significantly lower than that of 15% DMSO(0.261,0.340 respectively).The frozen-thawing sperm mobility(0.193) and recovery rate of sperm(0.250) keeping in 4 °C for 30 min were not significant different from that of 4 °C for 60 min(0.249,0.325 respectively)(P>0.05).But along with prolongation of the balance time(120~180 min),the frozen-thawing sperm mobility lowered obviously,and sperm was almost dead.In conclusion,rabbit semen can have better cryopreservation effect when it is keeping in extender 2,undr 4°C for 30~60 min.

以家兔为试验动物,从精液解冻后的精子活率和复苏率2个指标,分别对3种稀释液(1:T ris0.25 m o l/L+蔗糖0.1 m o l/L+柠檬酸钠83 mm o l/L+卵黄10%+二甲基亚砜(DM SO)15%;2:配方1+葡萄糖0.47 mm o l/L;3:T ris 0.29 m o l/L+葡萄糖0.71 mm o l/L+柠檬酸钠67 mm o l/L+卵黄20%+甘油4%)、两种抗冻剂(甘油4%、DM SO 15%)、4°C下平衡时间(30、60、120、180 m in)三个方面进行了比较试验。公兔采精用假阴道法,采精后立即检查精子活率,达0.60以上的供试验用。结果表明:(1)3种稀释液中,用稀释液2稀释的精液,精子解冻后活率和复苏率显著高于稀释液1和稀释液3(精子活率:0.153 vs 0.103、0.109;复苏率:0.205 vs 0.137、0.147,P<0.01),而稀释液1和稀释液3之间差异不显著(P>0.05);(2)在稀释液2中,分别用4%甘油和15%DM SO作防冻剂,两者的精子解冻后活率之间(0.193 vs 0.261,P<0.01),复苏率之间(0.250 v...

以家兔为试验动物,从精液解冻后的精子活率和复苏率2个指标,分别对3种稀释液(1:T ris0.25 m o l/L+蔗糖0.1 m o l/L+柠檬酸钠83 mm o l/L+卵黄10%+二甲基亚砜(DM SO)15%;2:配方1+葡萄糖0.47 mm o l/L;3:T ris 0.29 m o l/L+葡萄糖0.71 mm o l/L+柠檬酸钠67 mm o l/L+卵黄20%+甘油4%)、两种抗冻剂(甘油4%、DM SO 15%)、4°C下平衡时间(30、60、120、180 m in)三个方面进行了比较试验。公兔采精用假阴道法,采精后立即检查精子活率,达0.60以上的供试验用。结果表明:(1)3种稀释液中,用稀释液2稀释的精液,精子解冻后活率和复苏率显著高于稀释液1和稀释液3(精子活率:0.153 vs 0.103、0.109;复苏率:0.205 vs 0.137、0.147,P<0.01),而稀释液1和稀释液3之间差异不显著(P>0.05);(2)在稀释液2中,分别用4%甘油和15%DM SO作防冻剂,两者的精子解冻后活率之间(0.193 vs 0.261,P<0.01),复苏率之间(0.250 vs 0.340,P<0.01)差异极显著;(3)在4°C下平衡30、60、120、180 m in,结果发现,在30~60 m in之间,解冻后精子活率之间(0.261 vs 0.249),复苏率之间(0.340 vs 0.325)差异均不显著(P>0.05),但是随着平衡时间(120~180 m in)的增加,精子活率明显降低,精子几乎死亡。结论:采用稀释液2的配方,在4°C下平衡30~60 m in,可获得较好的兔精液冷冻保存效果。

 
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