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balance time
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  平衡时间
    The frozen-thawing sperm mobility(0.193) and recovery rate of sperm(0.250) keeping in 4 °C for 30 min were not significant different from that of 4 °C for 60 min(0.249,0.325 respectively)(P>0.05). But along with prolongation of the balance time(120~180 min),the frozen-thawing sperm mobility lowered obviously,and sperm was almost dead.
    (3)在4°C下平衡30、60、120、180 m in,结果发现,在30~60 m in之间,解冻后精子活率之间(0.261 vs 0.249),复苏率之间(0.340 vs 0.325)差异均不显著(P>0.05),但是随着平衡时间(120~180 m in)的增加,精子活率明显降低,精子几乎死亡。
短句来源
    When mouse seminiferous epithelial cells are ultrarapidly cryopreserved in a cryopeservative solution at a high concentration, the balance time should be shorter than 5 min, or operate at 4 ℃.
    在高浓度抗冻剂超速冷冻保存小鼠生殖细胞时 ,平衡时间应短于 5min,或在 4℃进行。
短句来源
    Influences of single factors including diluting liquid kinds,glycerin density,diluting means,low-temperature (4℃) balance time,heat-balance time and distance from liquid nitrogen on vitality of resuscitated sperm of 20 freezing semen specimens of ostrich per group were studied.
    对每组20份精子样品,采取不同冷冻程序,分析了各单因子如:稀释液种类、稀释液中甘油浓度、稀释方法、4℃低温平衡时间、冷冻面与液氮面距离及热平衡时间对鸵鸟冷冻精液的精子解冻复苏活率的影响。
短句来源
    The effects of different diluents,cryoprotectants and balance time on the rabbit semen cryopreservation were compared. The results were as followed.
    本试验采用不同的稀释液对兔精液进行冷冻保存,并比较了在稀释液中添加不同防冻剂以及平衡时间对兔精液冷冻保存效果的影响。
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  balance time
Two principal components of winter balance time-series explain about 50% of the variability in the data.
      
Significant improvements in knee extensor isometric strength (+21%), single leg balance time (+30%), and 6?min walk distance (+6%) were observed in TRA (P>amp;lt;0.05) but not in CON.
      
CW and indicators of functional capacity (knee extensor isometric strength, single leg balance time, sit and reach, stand and reach, and 6?min walk distance) were assessed prior to and following the intervention.
      
Following the practice sessions, the balance time increased significantly in the experimental group (P>amp;lt;0.01).
      
Only flunitrazepam 4?mg produced a significant decrease in balance time.
      
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Cryopreservation of murine embryo fibroblast and new bovine testicular fibroblast were investigated in this paper.The results showed as follows: The vitality of murine embryo fibroblast(MEF) cells and new bovine tisticular fibroblast(NBTF) cells exposed to the freezing media Ⅱ(PBS supplemented 0.4% BSA,1.5 mol/L ethylene glycol(EG),0.1 mol/L sucrose) higher than freezing media Ⅰ(10% NBS+10%DMSO+DMEM); When NBTF and MEF were freezed in media Ⅱ,the balanced time can be reduced from 2.5 h to 0.5 h without...

Cryopreservation of murine embryo fibroblast and new bovine testicular fibroblast were investigated in this paper.The results showed as follows: The vitality of murine embryo fibroblast(MEF) cells and new bovine tisticular fibroblast(NBTF) cells exposed to the freezing media Ⅱ(PBS supplemented 0.4% BSA,1.5 mol/L ethylene glycol(EG),0.1 mol/L sucrose) higher than freezing media Ⅰ(10% NBS+10%DMSO+DMEM); When NBTF and MEF were freezed in media Ⅱ,the balanced time can be reduced from 2.5 h to 0.5 h without influence on their vitality. Moreover,freezing rate can be increased from 0.6℃/min to 1.2℃/min,induced ice did not influence on their vitality.On the vitality of NBTF cells and MEF cells,thawing media Ⅱ(PBS supplemented 0.4% BSA,0.1 mol/L sucrose)>thawing media Ⅲ(DMEM supplemented 10% NBS)>thawing media ⅠPBS.

研究了冷冻保护液、平衡时间、降温速度、解冻液以及解冻方式对冷冻小鼠胎儿成纤维细胞和牛睾丸成纤维细胞活力的影响。结果表明 ,对小鼠胎儿成纤维细胞和犊牛睾丸成纤维细胞进行冷冻 ,冷冻液 ( 0 .4 %BSA) +1 .5mol/ L乙二醇 +0 .1 mol/ L蔗糖的 PBS液 )的冷冻效果优于冷冻液 ( 1 0 %的 NBS+1 0 %DMSO的 DMEM液 )。用冷冻液 对小鼠胎儿成纤维细胞和犊牛睾丸成纤维细胞进行冷冻 ,平衡时间可从 2 .5h缩短到 0 .5h;冷冻细管降温速度 ( -6.8~ -3 7℃ )可以从 0 .6℃ / min提高到 1 .2℃ / min;诱发结晶可以省略。 3种解冻液解冻细胞活力排序为 :解冻液 ( 0 .4 %BSA+0 .1 mol/ L蔗糖的 PBS) >解冻液 ( 1 0 %NBS的 DMEM) >解冻液 ( PBS)。用冷冻液 对 MEF和 BTF细胞冷冻过程中 ,无需用培养液制备细胞悬液反复离心

The survival rates of single cells from mouse seminiferous epithelia were measured by trypan blue exclusion after a definite time span in various cryopreservative solutions at 20 ℃. Dimethyl sulphoxide (DMSO), propanediol(PG) or ethylene glycol(EG) at concentration of 10% at 20 ℃ all show no significant different effects on the survival rates of 7 day old mouse seminiferous epithelial cells compared with control, while glycerol(G) significantly decreased the survival rates. DMSO, PG, EG and G at concentration...

The survival rates of single cells from mouse seminiferous epithelia were measured by trypan blue exclusion after a definite time span in various cryopreservative solutions at 20 ℃. Dimethyl sulphoxide (DMSO), propanediol(PG) or ethylene glycol(EG) at concentration of 10% at 20 ℃ all show no significant different effects on the survival rates of 7 day old mouse seminiferous epithelial cells compared with control, while glycerol(G) significantly decreased the survival rates. DMSO, PG, EG and G at concentration of 10% all show no significant different effects on the survival rates of adult mouse male germ cells compared with control. DMSO, PG, EG and G at concentration of 25% all significantly decreased the survival rates of both 7 day old mouse seminiferous epithelial cells and adult mouse male germ cells. The results indicate that DMSO, PG and EG are likely cryoprotectants for slow cryopreservation of 7 day old mouse seminiferous epithelial cells. DMSO, PG, EG and G at concentration of 10% are likely cryoprotectants for slow cryopreservation of adult mouse male germ cells. When mouse seminiferous epithelial cells are ultrarapidly cryopreserved in a cryopeservative solution at a high concentration, the balance time should be shorter than 5 min, or operate at 4 ℃.

将小鼠生精上皮单细胞置 2 0℃含不同抗冻剂的冻存液中一定时间后 ,台盼蓝染色测定细胞存活率 ,以筛选冷冻保存小鼠生精上皮细胞的侯选抗冻剂及使用浓度。结果 ,2 0℃ 3 0min,1 0 %浓度的二甲基亚砜 ( DMSO)、丙二醇( PG)及乙二醇 ( EG)对 7日龄小鼠生精上皮单细胞存活率均无显著影响 ,而 1 0 %甘油 ( G)则使细胞存活率显著下降 ;2 0℃ 3 0 min,1 0 %浓度的 DMSO、PG、EG及 G对成年小鼠生精上皮单细胞存活率均无显著影响 ;2 0℃ 5min,2 5%浓度的 DMSO、PG、EG及 G均使 7日龄及成年小鼠生精上皮单细胞存活率显著下降。实验结果表明 ,1 0 % DMSO、PG、及 EG可作为 7日龄小鼠生殖细胞慢速冷冻保存时的侯选抗冻剂 ,1 0 % DMSO、PG、EG及 G可作为成年小鼠生殖细胞慢速冷冻保存时的侯选抗冻剂 ;在高浓度抗冻剂超速冷冻保存小鼠生殖细胞时 ,平衡时间应短于 5min,或在 4℃进行。

Influences of single factors including diluting liquid kinds,glycerin density,diluting means,low-temperature (4℃) balance time,heat-balance time and distance from liquid nitrogen on vitality of resuscitated sperm of 20 freezing semen specimens of ostrich per group were studied.Result showed:two steps diluting ostrich semen with 2.8% glycerin density of MEM/yolk diluted liquid,balancing 2.5 h at low-temperature(4℃)and heat-balancing 2 min from liquid nitrogen 1.5 cm were the better effect...

Influences of single factors including diluting liquid kinds,glycerin density,diluting means,low-temperature (4℃) balance time,heat-balance time and distance from liquid nitrogen on vitality of resuscitated sperm of 20 freezing semen specimens of ostrich per group were studied.Result showed:two steps diluting ostrich semen with 2.8% glycerin density of MEM/yolk diluted liquid,balancing 2.5 h at low-temperature(4℃)and heat-balancing 2 min from liquid nitrogen 1.5 cm were the better effect to vitality of resuscitated sperm of freezing ostrich semen.

对每组20份精子样品,采取不同冷冻程序,分析了各单因子如:稀释液种类、稀释液中甘油浓度、稀释方法、4℃低温平衡时间、冷冻面与液氮面距离及热平衡时间对鸵鸟冷冻精液的精子解冻复苏活率的影响。结果表明:以含甘油浓度2.8%的(MEM·蛋黄)稀释液,采用两步稀释,低温平衡2.5h,冷冻面与液氮面距离1.5cm进行2min热平衡,鸵鸟精液的冷冻效果较好。

 
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