METHOD LUNA C 18(2) column was used. The acetonitrile :0.0 5moL·L -1 sodium phosphate monobasic solution (25∶75) was used as the mob ile phase. The flow rate was 1.0mL·min -1.The detection wavelength was set as 278nm.
The running buffer was 0.2 mol·L -1 Tris 40 mmol·L -1 sodium phosphate monobasic 20% isopropanol, adjusted pH to 5.5 with phosphoric acid. The wavelength of detection was 205 nm.
HPLC method is used for the determination of matrine in Compound radix euphorbiae fischerianae injection with the column of Shim pack CLC ODS, the mobile phase of acetonitrile 0.02 mol·L -1 sodium phosphate monobasic (60∶40), detection wavelength of 210 nm.
A mobile phase consisting of acetonitrile-20 mmol·L~(-1) sodium phosphate monobasic(containing Triethylamine(0.4%),pH(6.5))(50∶50) was used with a flow rate of(1.0 mL)·min~(-1),Ex was 290 nm,Em was 485 nm.
MethodsThe Chromatography conditions include: Chromatography column: Nova pak C 18 (3.9mm×150mm,4μm), mobile phase: 0.05mol/L sodium phosphate monobasic methanol water(0.5∶7∶92.5), UV detection at 260nm, FUDR was extracted with ethyl acetate.
METHODS A reversed phase column packed with C18(5 μm) was used. The mobile phase w as phosphate buffer (0.08% sodium phosphate monobasic,pH=4.7)-methanol ( 9∶1,v/v) and the flow rate was 1.5 ml·min-1,the dection wavel ength was 254 nm and metronidazole as internal standard.
Methods: The sample was determined by RP-HPLC. The mobile phase consisted of 80%acetonitrile-equimultiple proportion mixed solution0.01 mol·L-1 sodium sulfonic acid octane and 0.02 mol·L-1 sodium phosphate monobasic (10%phosphate mixed the solution pH 2.8)(25:75). The flow rate was 1.0 mL·min-1. The detection wavelength was at 254nm.
It uses reverse-phase HPLC on a μ Bondapak C18 column with a flourescence detector. The mobile phase is consisted of 5 m mol/L sodium phosphate monobasic, acetonitrile and triethylamine. The minimum of detection is 1. 08 μg/mL.
The effects of different surfactants(sodium dodecyl sulfate,sodium cholate and sodium deoxycholate),different electrolytes(sodium phosphate monobasic,sodium tetraborate and3-cyclohexylamino-1-propane sulfonic acid),buffer pH and organic modifiers(methanol and acetonitrile)on the determination of sialic acid were studied. The effects of the composition of sample matrix and injection time on the concentration efficiency of sialic acid were also studied.
Effects of several factors,such as the pH value and concentration of the running buffer,the concentration of Tween-20,separation voltage,injection time and the potential applied to the working electrode,were investigated to find the optimum conditions. Well-defined separation of TC and OTC was achieved in the running sodium phosphate monobasic(pH 2.6).
A RP HPLC method was developed to determine the concentrations of sinomenine HCl in serum and urine and its pharmacokinetics was studied in healthy volunteers. C 18 H 37 column was eluted with the mobile phase of acetonitrile—0 01 mol·L -1 sodium phosphate monobasic? ,N,N',N' tetramethylenediamine (46∶54∶0 22 v/v , pH 6 9) and the utraviolet absorbance was monitored at 263 nm. Triazolan was used as internal standard. The calibration curves were linear in the range o...
A simple, reliable method for morphine determination in biological samples has been developed. It uses reverse-phase HPLC on a μ Bondapak C18 column with a flourescence detector. The mobile phase is consisted of 5 m mol/L sodium phosphate monobasic, acetonitrile and triethylamine. The minimum of detection is 1. 08 μg/mL. The recovery rate is 58 %.
HPLC method is used for the determination of matrine in Compound radix euphorbiae fischerianae injection with the column of Shim pack CLC ODS, the mobile phase of acetonitrile 0.02 mol·L -1 sodium phosphate monobasic (60∶40), detection wavelength of 210 nm. Results: The linear range is 0.15~0.90 mg·ml -1 ,γ=0.999 3, the average recovery is 99.13% and RSD=0.99%. The method is simple, rapid and accurate, it can be a quality control standard of Compound Radix Euphorbiae fischeria...