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bt toxin
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  bt毒素
     Relationships between aminopeptidase N—the receptor for Bt toxin in Helicoverpa armigera and Bt resistance
     棉铃虫Bt毒素受体蛋白—氨肽酶N与抗性的关系
短句来源
     Gene Cloning of Aminopeptidase N1-Receptor for Bt Toxin in Helicoverpa Armigera (Hübner) and Its Expression in Tn Cells
     棉铃虫Bt毒素受体蛋白—氨肽酶N1基因克隆及其在昆虫细胞系的表达
短句来源
     Bt toxin distribution in transgenic Bt cotton and soil system.
     Bt毒素在转基因棉花与土壤系统中的分布
短句来源
     Estimated frequency of resistance alleles to Bt toxin Cry1Ac in the field populations of Helicoverpa armigera (Hübner) from Northern China
     棉铃虫田间种群Bt毒素Cry1Ac抗性基因频率的估算
短句来源
     This study showed that the amounts of Bt toxin expressed in transgenic Bt cotton leaves and stems (103.5~134.1 ng·g~(-1)) were rather higher than those expressed in transgenic Bt cotton roots (44.7~21.2 ng·g~(-1)),indicating that total amount of soil Bt toxin introduced by transgenic Bt cotton could be decreased through treating its above-ground biomass.
     研究了转Bt基因棉花与土壤系统中Bt毒素的分布. 结果表明,两种转Bt基因棉花地上部(叶片、茎秆)的毒素表达量(103.5~134.1 ng.g-1)显著高于地下部分(根系)(44.7~21.2 ng.g-1),土壤中Bt毒素总量可通过转基因棉花地上部分秸秆的处理得到控制;
短句来源
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  bt毒蛋白
     Expression of Bt Toxin Receptor Gene (Bt-R3) in E.coli. and Structure and Function of Bt-R3 Protein
     Bt毒蛋白受体基因Bt-R3在大肠杆菌中的表达及受体蛋白结构和功能的初步研究
短句来源
     Expression Behavior of Bt Toxin Gene in Transgenic Tobaccos
     转基因烟草中Bt毒蛋白基因的表达行为
短句来源
     By using 125I-labeled Bt toxin, the differences of binding kinetics among CrylAa, CrylAb, Cry 1 Ac and BBMV in larval midgut of resistant and susceptible H. armigera were tested.
     利用~(125)I标记Bt毒蛋白,测定了棉铃虫抗、感品系幼虫中肠BBMV与Cry1Aa、Cry1Ab和Cry1Ac结合动力学的差异。
短句来源
     Transgenic Corn with Bt Toxin Protein Gene and Insect-resistance
     转Bt毒蛋白基因玉米及其抗虫性研究进展
短句来源
     Effects of Bt toxin Cry1Ac on biochemical responses of Eisenia fetida in an artificial soil
     Bt毒蛋白Cry1Ac在人造土壤中对赤子爱胜蚓毒理及生化影响
短句来源
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  bt杀虫
     WT5BZ]1.0 kb and 2.4 kb DNA fragment containing rps7 gene and ndhB gene, respectively, were cloned from oilseed rape chloroplast genome. A full length 3.5 kb Bt toxin gene ( cry1Aa ) was also cloned from the plasmid of Bacillus thuringiensis.
     首先从油菜叶绿体基因组克隆得到包含rps7基因在内的 1.0kbDNA片段和包含ndhB基因在内的 2 .4kbDNA片段 ,同时从苏云金芽孢杆菌质粒上克隆得到一个全长 3.5kb的Bt杀虫蛋白基因cry1Aa。
短句来源
     The southern blotting show Bt toxin gene in 8 insect-resistant trangenetic cotton harbing single gene lines and 和5 insect-resistant trangenetic cotton harbing double gene lines is 1 to 4 copies respectively.
     通过Southern杂交检测到Bt杀虫基因在8个单价转基因抗虫棉和5个双价转基因抗虫棉基因组中整合的拷贝数为1至4。
短句来源
     The vector containing Bt toxin gene and aadA gene for oilseed rape chloroplast transformation was constructed with the rps7 gene and ndhB gene as homologous recombinant fragments.
     然后以rps7和ndhB基因作为同源重组片段 ,成功构建了包含Bt杀虫蛋白基因和aadA抗壮观霉素基因的油菜叶绿体定点转化载体 ,并对克隆菌体总蛋白进行了生物杀虫试验。
短句来源
     There are two HindIII restriction sites at each end of the Bt toxin gene in the vectorpGBI 121 S4ABC. Southern blot analysis of the GK 139-20 R3 insect-resistant transgeniccotton plants indicates that the Bt toxin gene introduced by pollen tube pathway has beenintegrated into the genome of the cotton.
     利用载体pGBI121S4ABC中杀虫基因两端的HindIII酶切位点,对Bt抗虫棉GK139-20的R3、R4代材料进行Southern杂交分析,结果证明了利用花粉管通道法导入的Bt杀虫基因整合在抗虫棉GK139-20 R3、R4代材料基因组中。
短句来源
     The antigen and antiserum of crystal toxin protein of Bacillus thuringiensis srtains HD-1 and HD-68 which were soluble in alkali were prepared. A Dot-ELISA for Bt toxin protein had been developed. The detectable level of Bt toxin protein by Dot-ELISA was 87. 5ng/ml.
     用苏云金芽孢杆菌HD-1和HD-68晶体杀虫蛋白的碱溶产物作为抗原,制备相应的 抗血清,建立了检测 Bt杀虫蛋白的 Dot-ELISA,可检测 Bt杀虫蛋白的最低水平为 87.5ng/ml。
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  “bt toxin”译为未确定词的双语例句
     A solution of 0.1M Na2CO3-NaHCO3+5mM DTT effectively extracted most of Bt toxin presented in the tissue of crylAb transgenic rice.
     采用0.1M Na_2CO_3-NaHCO_3+5.0mM DTT缓冲液作为提取剂以提取克螟稻秸秆中的Bt蛋白。
短句来源
     The minimal detectable concentration of purified Bt toxin was 1μg·L -1 and the linear assay range of the method was 1μg·L -1 to 15μg·L -1 .Both the errors of intra and interplate(s) were <5%,and the mean recovery rate was 94.94%.
     检测极限1μg·L- 1,线性范围1- 15μg·L- 1,板内误差C.V.< 5% ,板间误差C.V.< 5% ,平均样品回收率94.94% 。
短句来源
     In pBinMoBc, cry 1Ac3 gene, which encodes the Bt toxin, is under the control of chimeric OM promoter. In pBinoBc, it is under control of CaMV 35S promoter.
     pBinMoBc携带有高效启动子复合OM启动子控制下的cry1Ac3基因 ,pBinoBc携带有 35S启动子控制下的cry1Ac3基因。
短句来源
     The study on the photosynthetic characters and Bt toxin content of different Bt cottons(ZK,GK)and non-Bt cotton(CZ)indicated that Bt cottons GK and ZK had different total expressed amount of Bt toxin and its distribution in different tissues.
     转Bt基因棉花 (GK、ZK)及非Bt基因棉花 (CZ)杀虫晶体蛋白表达及光合特性的研究表明 ,杀虫晶体蛋白在转Bt基因棉花GK与ZK中的表达总量及在各器官中的分配均有所不同 .
短句来源
     In tobacco capsula, the average Bt toxin protein expression level of A line and B line was 1.5 times and 2.4 times than CK respectively. In tobacco capsular hull, the toxin expression level of A and B was 1.5 times and 1.9 times compared with CK respectively;
     在烟草的蒴果中,A系的表达量是CK系的1.5倍,B系的表达量是CK系的2.4倍:在烟草的蒴果壳中,A系的表达量是CK系的1.5倍,B系的表达量是CK系的1.9倍;
短句来源
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  bt toxin
Deterrence index (DI) of larvae decreased in later instars, which indicated that the Bt toxin decreased with age.
      
The results indicated that Bt toxin was not the direct factor causing decrease of the numbers of bacteria in the rhizosphere, and other factors may be involved.
      
Fortification of pure Bt toxin into rhizospheric soil did not result in significant changes in the numbers of culturable functional bacteria, except the nitrogen-fixing bacteria when the concentration of Bt toxin was higher than 500?ng/g.
      
The levels of Bt toxin in the rhizosphere of NuCOTN99B were significantly higher (p>amp;lt;0.05) than those of SGK321 within all sampling dates except on June 17th in the whole growth season.
      
No Bt toxin was found in the rhizosphere of non-Bt cotton (SHIYUAN321), but varying levels of Bt toxin were present in the rhizosphere of two Bt cotton varieties (NuCOTN99B and SGK321) during the entire growth period.
      
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By in situ hybridization of bacterium clone and analysis of restriction enzyme digestion, both CMV cp gene and Bt toxin gene were inserted one by one into T DNA of binary plant expression vector pE 3 .The reconstructed plasmid was named pE 14 .Then, tomato was transformed with pE 14 mediated by Agrobacterium tumefaciens GV311 SE, four regenerated tomato plants were obtained on the MS medium containing 100 μg/mL kanamycin. Assay of...

By in situ hybridization of bacterium clone and analysis of restriction enzyme digestion, both CMV cp gene and Bt toxin gene were inserted one by one into T DNA of binary plant expression vector pE 3 .The reconstructed plasmid was named pE 14 .Then, tomato was transformed with pE 14 mediated by Agrobacterium tumefaciens GV311 SE, four regenerated tomato plants were obtained on the MS medium containing 100 μg/mL kanamycin. Assay of nopaline, dot blotting of tomato genomic DNA and PCR amplication of CMV cp gene and Bt toxin gene from genomic DNA showed that CMV cp gene and Bt toxin gene were transferred into the four regenerated tomato plants simultaneously with T DNA, and no recombination of genes occurred. RNA dot blotting showed that two of them could express simultaneously the CMV cp gene and Bt toxin gene proteins. The resistances to virus and insect of the transgenic tomato plants will be tested in their F 1 and F 2 regenerations.

将抗病毒的CMV-cp 基因和抗虫的Bt-toxin 基因依次插入到植物表达载体pE3 的HindⅢ和KpnⅠ位点,通过菌落原位杂交筛选和酶切鉴定,然后以土壤农杆菌GV311-SE介导转化番茄,胭脂碱检测,染色体DNA 的点杂交及PCR扩增证明CMV-cp 基因和Bt-toxin 基因已同时导入转化再生的番茄植株。RNA 点杂交证明CMV-cp 基因和Bt-toxin 基因已在转基因番茄植株中同时获得表达。

Expression vector pE14 with double resistance to virus and insect was constructed by inserting CW-cp gene and Bt-toxin gene one by into T-DNA of the same binary vector pE3. Tobacco was then transformed with Agrbacterium tumefaciens (At)GV311-SE carrying PE14. Nopaline assay and PCR amplification confirmed that both CW-cp gene and Bt-toxin gene had been introduced into tobacco genome by T-DNA of PE3. Test attack with virus and demonstrated, in some of the transgenic plants, double resistance...

Expression vector pE14 with double resistance to virus and insect was constructed by inserting CW-cp gene and Bt-toxin gene one by into T-DNA of the same binary vector pE3. Tobacco was then transformed with Agrbacterium tumefaciens (At)GV311-SE carrying PE14. Nopaline assay and PCR amplification confirmed that both CW-cp gene and Bt-toxin gene had been introduced into tobacco genome by T-DNA of PE3. Test attack with virus and demonstrated, in some of the transgenic plants, double resistance to both infection by CMV and damage by Manduca sexta.

GeneticEngineeringofTobaccowithDoubleResistancetoBothVirusandInsectLIANGXiao-you;(梁晓友)MIJing-jiu;(米景九),PanNai-sui(潘乃隧),CHENzh...

Plant expression vector pE14 with double resistances to virus and insect has obtained by insert-ing CMV-cp gene and Bt-toxin gene into the T-DNA of a same Agrobacterium tumefaciens(A.t)binilry vec-tor pE3 one by one,Tobacco was transformed with A.t0.GV311-SE containing pE14.The Nopaline assay andthe PCR amplification of CMV-cp gene and Bt-toxin gene from regenerated plants indicate that CMV-cp geneand Bt-toxin gene have been introduced into tobacco genome by T-DNA of pE3.Virus...

Plant expression vector pE14 with double resistances to virus and insect has obtained by insert-ing CMV-cp gene and Bt-toxin gene into the T-DNA of a same Agrobacterium tumefaciens(A.t)binilry vec-tor pE3 one by one,Tobacco was transformed with A.t0.GV311-SE containing pE14.The Nopaline assay andthe PCR amplification of CMV-cp gene and Bt-toxin gene from regenerated plants indicate that CMV-cp geneand Bt-toxin gene have been introduced into tobacco genome by T-DNA of pE3.Virus and insect bioasssaysshow that some transgenic plants can resistance the infecthn of CMV and the damage of Manduca sexta.

通过将抗病的CMV-cp基因和抗虫的Bt-toxin基因分别加上GaMV35S启动子和终止子,依次插入到同一植物表达载体pE3的T-DNA上,获得双抗载体pE14。然后载体pE14以土壤农杆菌GV311-SE介导转化烟草,再生植株的胭脂碱检测及PCR扩增证明CMV-CP基因和Bt-toxin基因已随T-DNA同时导入烟草植株中。抗病抗虫实验表明转基因植株具有较强的抗病毒CMV的能力和抗烟青虫的能力。

 
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