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rds mice
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  rds小鼠
     MATERIALS:The rd mice,rds mice and passage mice from C3B mice as normal control were purchased from Jackson Laboratory(Bar Harbor,Maine 04609,USA),and raised in second class unit of Experimental Animal Center of Sun Yat-sen University.
     材料:实验用rd小鼠和rds小鼠以及正常对照的C3B小鼠传代小鼠模型购自Jackson实验室(巴港,缅因州04609,美国),所有动物均在中山大学实验动物中心二等动物单元饲养。
短句来源
     The layer numbers of retinal photoreceptor cells at equator posterior center was counted under light microscopy. Results Result from light microscopy showed that the layer numbers of retinal photoreceptor cells in the tetramethylpyrazine treatment group were increased in 14, 21, 28, and 35 d in rd and rds mice, compared with the control group (P<0.01).
     结果病理结果显示,经盐酸川芎嗪治疗后14、21、28、35d,与未用药的对照组相比较,rd和rds小鼠光感受器细胞层数明显增加(P<0.01)。
短句来源
     The retinal function changes were detected by Electroretinogram (ERG). The mRNA expressions of bax, bcl-2, caspase-3 and c-fos were determined by real-time fluorescent quantitative (FQ)-PCR method in rds mice treated with shihusan.
     用real-time荧光定量PCR的方法研究石斛散治疗rds小鼠后各时间点凋亡相关基因bax、bcl-2、Caspase-3和c-fos的变化。
短句来源
     Conclusion The results from light microscopy suggest that tetramethylpyrazine has a significant efficacy to the retinal photoreceptor cells of rd and rds mice.
     结论川芎嗪可延缓rd和rds小鼠视网膜光感受器细胞的破坏。
短句来源
     Objective To determine whether tetramethylpyrazine hydrochlorde has an efficacy on hereditary retinitis pigmentosa of rd and rds mice by the observation of the photoreceptor cells with light microscopy.
     目的通过rd和rds小鼠视网膜光感受器细胞的病理变化探讨盐酸川芎嗪对遗传性视网膜色素变性小鼠可能的治疗作用。
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  rds鼠
     Methods Proteins, prepared from the retinas of rds mice and normal C3B mice at different ages, were separated by using two dimensional electrophoresis and then analyzed by 2 DE imaging analyzer.
     方法 分别取不同时期的 rds鼠和正常 C3B鼠视网膜 ,用双向电泳(2 - DE)技术 ,对比、分析其表达蛋白质点的差异 ,寻找 rds鼠视网膜中与色素变性相关的蛋白质。
短句来源
     Objective To apply two dimensional (2 D) gel electrophoresis to resolve specially expressed proteins related to retinitis pigmentosa (RP) in the retina of rds mice.
     目的 应用蛋白质组分析技术 ,分离 rds鼠视网膜中与视网膜色素变性 (RP)相关的特异表达蛋白质。
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  “rds mice”译为未确定词的双语例句
     Methods Eighty-four rd and rds mice were randomly divided into two groups, experimental group and control group. Each group had 42 mice.
     方法rd和rds新生鼠各84只,随机分为两组,实验组和对照组,每组42只小鼠。
短句来源
     The expression of calpain Ⅱ in the retina of rds mice
     细胞因子Ⅱ在遗传性视网膜色素变性的转基因小鼠视网膜的表达研究
短句来源
     Compared with the control groups, we found that only fraction 4 decreased the lung coeffcient of RDS mice with dose dependent manner and prevented the RDS rabbits from the decrease in PaO_2 of blood and the protein exudation.
     结果显示:与对照组相比,组份4使实验组小白鼠的肺系数降低且呈量—效关系; 使实验组家兔PaO_2下降幅度减少;
短句来源
     Compared with the control groups,we found that only fraction Ⅳ decreased the lung coefficient of RDS mice in a dose dependent manner and ameliorate the decrease in blood PaO_2 and the protein exudation of RDS rabbits.
     结果显示:与对照组相比,组份Ⅳ使实验组小鼠的肺系数降低且呈量-效关系; 使实验组家兔PaO2下降幅度减少,支气管-肺泡灌洗液蛋白渗出量减少;
短句来源
     Methods Forty-two rds mice were sacrificed at different time point postnatal 0 day,3 days,7 days,14 days,21 days,28 days,35 days. Eleven eyeballs were enucleated and immediately fixed with 10%(volume fraction) neutral formaldehyde. The thickness of retinal outer nuclear layer cells at the equator of the horizontal eyeball was measured by light microscope.
     方法rds新生鼠42只,在0d、3d、7d、14d、21d、28d、35d分别取11个眼球,立即经体积分数10%中性甲醛固定,光学显微镜下观察眼球水平位视网膜赤道部外核层细胞的厚度;
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  rds mice
The finding that rom-1 transports to the outer segment domain in rds mice suggests that rom-1 may possess its own sorting and transport signals.
      
Opsin gene expression during early and late stages of retinal degeneration in rds mice.
      
In developing rds mice, rom-1 accumulated primarily in distal ciliary membranes.
      
Finally, we examined rds mice to investigate whether rom-1 localizes properly in the absence of peripherin/rds.
      
At P21, before treatment, rds/rds mice yielded rod-mediated ERGs with severely impaired responses.
      


Four protein fractions were separated from venom of Naja naja atra with chromatograph on DEAE Sephadex A50 column and their effects were studied on animals with respiratory distress syndrome (RDS) induced by oleic acid. Compared with the control groups, we found that only fraction 4 decreased the lung coeffcient of RDS mice with dose dependent manner and prevented the RDS rabbits from the decrease in PaO_2 of blood and the protein exudation. Because of the activity of this fraction 4 with anticomplement,...

Four protein fractions were separated from venom of Naja naja atra with chromatograph on DEAE Sephadex A50 column and their effects were studied on animals with respiratory distress syndrome (RDS) induced by oleic acid. Compared with the control groups, we found that only fraction 4 decreased the lung coeffcient of RDS mice with dose dependent manner and prevented the RDS rabbits from the decrease in PaO_2 of blood and the protein exudation. Because of the activity of this fraction 4 with anticomplement, it is suggested that the anti-RDS effect of cobra venom is responsible for this fraction.

用DEAE Sephadex A50层析柱把眼镜蛇毒分离得4个组份,分别试用于动物的油酸型RDS模型。结果显示:与对照组相比,组份4使实验组小白鼠的肺系数降低且呈量—效关系;使实验组家兔PaO_2下降幅度减少;支气管—肺泡灌洗液蛋白渗出量减少:组份4还显示具有抗补体活性的作用,其缓解油酸型RDS的作用可能与此有关。

Four protein fractions were separated from Naja naja atra venom on DEAE Sephadex A50 chromatographic column.Their effects on animals with respiratory distress syndrome(RDS) induced by oleic acid were studied.Compared with the control groups,we found that only fraction Ⅳ decreased the lung coefficient of RDS mice in a dose dependent manner and ameliorate the decrease in blood PaO_2 and the protein exudation of RDS rabbits.Because of the anticomplement activity of fraction Ⅳ, it was suggested...

Four protein fractions were separated from Naja naja atra venom on DEAE Sephadex A50 chromatographic column.Their effects on animals with respiratory distress syndrome(RDS) induced by oleic acid were studied.Compared with the control groups,we found that only fraction Ⅳ decreased the lung coefficient of RDS mice in a dose dependent manner and ameliorate the decrease in blood PaO_2 and the protein exudation of RDS rabbits.Because of the anticomplement activity of fraction Ⅳ, it was suggested that this fraction is responsible for the anti-RDS effects of cobra venom.

用DEAESephadexA50层析柱把眼镜蛇毒分离得4个组份,分别试用于动物的油酸型呼吸窘迫综合征(RDS)模型。结果显示:与对照组相比,组份Ⅳ使实验组小鼠的肺系数降低且呈量-效关系;使实验组家兔PaO2下降幅度减少,支气管-肺泡灌洗液蛋白渗出量减少;组份Ⅳ具有抗补体活性的作用,其缓解油酸型RDS的作用可能与此有关。

Objective To study some possible apoptosis mechanisms of the process of retinal degeneration in rds mice. Methods The retina of rds and C3B mice were collected at different time periods to form the experimental and control groups respectively. By using immunohistochemical technique, the expressions of calpain in the retina of rds and C3B mice at different time periods were analyzed. Results The expression of calpain could be seen in the ganglionic layer of rds mice in the...

Objective To study some possible apoptosis mechanisms of the process of retinal degeneration in rds mice. Methods The retina of rds and C3B mice were collected at different time periods to form the experimental and control groups respectively. By using immunohistochemical technique, the expressions of calpain in the retina of rds and C3B mice at different time periods were analyzed. Results The expression of calpain could be seen in the ganglionic layer of rds mice in the early stage(l week),on the inner nuclear at about 2 weeks, outer nuclear layer at 4 ~ 5 weeks. The intense and scope increase with the growth of retina. After 4 weeks, the expression of calpain Ⅱ showed all of the rest of retina. Conclusion High calcium concentration activated the expression of Calpain Ⅱ. Therefore, high calcium concentration may be a factor in increasing the apoptosis of the retinal cells in the rds mice. The apoptosis may start from the ganglion cell layer in the rds mice.

目的探讨细胞因子Ⅱ(calpain Ⅱ)在视网膜色素变性过程中表达的变化,以此窥视遗传性视网膜色素变性的转基因小鼠( rds 小鼠)发病的某些可能因素。方法以 C3 B 小鼠为对照,选择不同生长时期的rds小鼠的视网膜组织,用免疫组化方法检测Calpain Ⅱ在不同生长阶段的小鼠模型视网膜中的是否表达及表达情况。结果1周即可见Calpain Ⅱ在rds小鼠视网膜神经节细胞层的表达, 2周左右内核层也见表达, 4周后外核层也开始出现由强至弱的calpain Ⅱ强阳性表达,最终至全部视网膜剩余组织中均可见calpain Ⅱ的表达。结论只有高钙才能激活calpain Ⅱ的表达,研究中calpain Ⅱ参与了rds小鼠视网膜细胞的变性死亡。因此,高钙是rds小鼠视网膜变性的一个可能因素。

 
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