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binding equilibrium
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  结合平衡
     Study on the Binding Equilibrium between Cd~(2+) and BSA with Excel Program Solving Method
     Excel规划求解法研究Cd~(2+)与BSA的结合平衡
短句来源
     Study on Binding Equilibrium between Mn(II) and HAS or BSA
     Mn(II)与HSA或BSA的结合平衡研究
短句来源
     Binding Equilibrium Study Between Co(Ⅱ), La(Ⅲ) and HSA or BSA
     Co(Ⅱ)、 La(Ⅲ)与HSA或BSA的结合平衡研究
短句来源
     Binding Equilibrium Study between Ni(Ⅱ) and HSA or BSA
     Ni(Ⅱ)与HSA或BSA的结合平衡研究
短句来源
     Binding Equilibrium Study Between La(Ⅲ) and HSA or BSA
     La(Ⅲ)与HSA或BSA的结合平衡研究
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  键合平衡
     SIMULATION FOR BINDING EQUILIBRIUM OF IONIC DERIVATIVE OF PYRENE WITH POLYELECTROLYTE
     离子型芘衍生物同聚电解质键合平衡的模拟
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  “binding equilibrium”译为未确定词的双语例句
     The research results indicate that the combination reaction of them is a single static quenching process, camptothecin derivatives strongly bind BSA with the molar ratio of 1∶1, the binding equilibrium constants(K_ 0 ) are as follows, K_ 0,7-ECPT =3.69×10~ 5 L/mol, K_ 0,10-HCPT =8.00×10~ 5 L/mol .
     实验表明,喜树碱类药物与BSA的相互结合作用为单一的静态猝灭过程,在溶液中二者以摩尔比1∶1牢固结合,其结合反应的平衡常数K0分别为:K0,7-ECPT=3·69×105L·mol-1、K0,10-HCPT=8·00×105L·mol-1。
短句来源
     The maximum binding number (N=168) and the binding equilibrium constant (K=3.25×106) of the alginic sodium diester and azur A were obtained by the theoretical model.
     通过理论模型测得AA与ASD最大结合数N=168,结合常数K=3.25×106。
短句来源
     The research results indicate that the combination reaction of them was a single static quenching process, and camptothecin derivatives strongly bound BSA with the molar ratio of 1∶1 and the binding equilibrium constant K0 at 25 ℃, K0,THC=7.73×105 L/mol, K0,IHC=4.73×105 L/mol.
     实验表明喜树碱类药物与牛血清白蛋白的相互结合作用为单一的静态猝灭过程,在溶液中二者以物质的量比1∶1牢固结合,25℃时其结合反应的平衡常数K0分别为:K0,THC=7.73×105L? mol-1,K0,IHC=4.73×105L?
短句来源
     The maximum binding number( N =54) and the binding equilibrium constant( K =1.563×10 6) of the sulfation pachymaran and methylene blue (MB) were obtained by the exoterica model. The influence of the molar ratio of MB/SP, ethanol, hydroxypropyl- β -cyclodextrin, Triton X-100 and NaCl on the spectra of MB-SP complex was investigated.
     通过理论模型测得亚甲蓝(MB)与SP最大结合数N =5 4 ,结合常数K =1 .5 6 3×10 6,考察了反应体系中MB/SP摩尔比、NaCl、乙醇、羟丙基 β环糊精以及TritonX 10 0对相互作用的影响。
短句来源
     CONCLUSION: HPCE/FA is a fast and high efficient analytical method which determine unbound chiral drug concentrations enantioselectively under drug protein binding equilibrium by ultramicro injection volume, and hence is useful especially for the binding study of proteins which are expensive and difficult to obtain.
     结论 :HPCE/ FA是一种高效、分析迅速、样本用量极少的研究蛋白结合方法 ,特别是对于手性药物与昂贵不易获得的蛋白结合研究
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  binding equilibrium
Moreover, measurements of the rate constants indicate that binding equilibrium at 37?C is achieved within seconds for all FA and FABPs.
      
The binding equilibrium was likewise attained faster at this temperature than that at lower temperature of 4°C for all studied groups.
      
We have used fluorescence detection to describe the binding equilibrium and kinetics of the Tet repressor interaction with the 20-bp DNA operators tet O1 and tet O2.
      
It attempts to identify more clearly the features that must be added to the idea of ligand binding equilibrium to simulate a weakly-coupled motor enzyme model.
      
By linking the conformational unfolding equilibrium to the proton binding equilibrium, a model is developed that is able to describe the dependence on thepH of the thermal denaturation processes of small globular protiens.
      
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The binding of cadmium ion to BSA and the pH effect Were studied by means of potential-restoration, fluorescence and viscosity studies. The results show that there may be two kinds of binding sites to cadmium (two strong and ten weak) .The binding of cadmium is significantly affected by variation of pH.In nearly neutral media,if Cd binding is not saturated, there is linear relation between the logarithm of number of moles of Cd bound per mole of BSA and the logarithm of free Cd ion concentration. Further treatment...

The binding of cadmium ion to BSA and the pH effect Were studied by means of potential-restoration, fluorescence and viscosity studies. The results show that there may be two kinds of binding sites to cadmium (two strong and ten weak) .The binding of cadmium is significantly affected by variation of pH.In nearly neutral media,if Cd binding is not saturated, there is linear relation between the logarithm of number of moles of Cd bound per mole of BSA and the logarithm of free Cd ion concentration. Further treatment gives an equation N=K[Cd2+]m, with which the Cd -binding equilibrium may be estimated.In this equation,K and m are all functions of pH.The experimental results give information of the conforma-tional changes induced by the binding of proton or Cd(II). By means of gel chromatographic studies,the influences of Zn(II), Ca(II), Tween-80 and SDS were studied.The effective competition of Zn (II) may mobilize the BSA bound Cd(II).SDS inhibits the Cd-BSA binding.These actions may be relevant with conformation conversion.

本文用恢复电位法、荧光分光光度法及粘度法研究了镉(Ⅱ)与牛血清白蛋白(BSA)的配位平衡和pH的影响。结果表明BSA有两类镉结合部位(2个强结合和10个弱结合部位)。Cd—BSA配位反应受pH影响,在近中性pH范围Cd(Ⅱ)与BSA结合未接近饱和时,以CURFIT程序对结合曲线拟合发现,每摩尔BSA平均结合Cd(Ⅱ)摩尔数的对数值与游离镉浓度的对数间成线性关系,进一步用Marguardt法处理得到Cd(Ⅱ)与BSA结合平衡方程式:(?)=K[Cd~(2+)]~m,K和m均为pH函数。构象研究表明H~+和Cd(Ⅱ)的结合都能引起BSA构象改变。用凝胶色谱法测定了Zn(Ⅱ)、Ca(Ⅱ)、吐温—80和十二烷基硫酸钠(SDS)对Cd—BSA结合的影响。Z_n(Ⅱ)可以竞争Cd(Ⅱ)在BSA上结合部位使Cd(Ⅱ)活动化。SDS能够有效抑制Cd(Ⅱ)与BSA结合,这种作用可能与SDS引起BSA构象改变有关。

Stopped flow studies show that the binding equilibrium of emodin to bovine serum albumin (BSA) is established within 100ms. The absorption maxium of these compounds exhibit red shift upon binding and the absorbance enhanced. The fluorescence quenching associated with the binding of these compounds to BSA was used to measure binding constants, and the distance of Trp-212 to the first binding site were calculated, on the basis of the energy transfer between BSA and bound complexes....

Stopped flow studies show that the binding equilibrium of emodin to bovine serum albumin (BSA) is established within 100ms. The absorption maxium of these compounds exhibit red shift upon binding and the absorbance enhanced. The fluorescence quenching associated with the binding of these compounds to BSA was used to measure binding constants, and the distance of Trp-212 to the first binding site were calculated, on the basis of the energy transfer between BSA and bound complexes. An allosteric domain model of emodin-BSA complex was postulated.

用停留技术研究了大黄素与牛血清白蛋白(BSA)结合反应的动力学,发现在25℃,pH10.0时结合在100ms即可达到平衡.大黄素等7种蒽醌及黄酮类化合物与BSA结合后,可见区最大吸收波长红移,吸收强度增加.荧光光谱研究表明,这些化合物对BSA荧光有很强的猝灭作用.根据猝灭结果,求出了它们与BSA的结合常数,并基于F(?)rster非辐射能量转移机理,计算了它们的第一结合部位与BSA中212-色氨酸残基的距离.提出了蒽醌及黄酮类化合物与BSA形成复合物的结构模型.

Potentiometric experiments were carried out on the proton binding equilibria of FA extracted from a weathered coal and HA and FA extracted from a dark loessial soil. The affinity spectrum model was employed to treat the experimental data. The affinity spectrum model technique could "magnify" the heterogeneity of the proton binding equilibria, so it was useful for comparing and studying the characteristics of humic substances with similar properties. According to the affinity spectra, we also found...

Potentiometric experiments were carried out on the proton binding equilibria of FA extracted from a weathered coal and HA and FA extracted from a dark loessial soil. The affinity spectrum model was employed to treat the experimental data. The affinity spectrum model technique could "magnify" the heterogeneity of the proton binding equilibria, so it was useful for comparing and studying the characteristics of humic substances with similar properties. According to the affinity spectra, we also found that the direction of the titration could affect the properties of the equilibria of FA from the weathered coal, and the acidic functional groups contained in FA from the weathered coal were larger in quantity than those contained in HA and FA from the dark loessial soil.

AcidDissociationConstantsofFunctionalGroupsinHumicSubstances:I.AffinitySpectrumModelAnalysisofPotentiometricDataofFulvicandHu...

 
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