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mannan binding lectin
相关语句
  甘露聚糖结合凝集素
     Molecular cloning and characterization of mannan binding lectin C gene from Balb/c mice
     Balb/c小鼠甘露聚糖结合凝集素-C基因的克隆与鉴定
短句来源
     Objective To clone the intact cDNA encoding mouse mannan binding lectin(MBL)-A polypeptide.
     目的 克隆小鼠甘露聚糖结合凝集素 A(MBL A)基因的全长编码区cDNA。
短句来源
     Objective:To investigate the concentration of mannan binding lectin and active HbsAb for the freshmen in Hebei province and further assess their innate immunity.
     目的:检测河北地区大学一年级HbsAb阳性与HbsAb阴性新生血清甘露聚糖结合凝集素(MBL)的浓度,比较差异并评价其非特异性免疫功能。
短句来源
     Mannan binding lectin (MBL) is a serum protein of hepatic origin belonging to a family of Ca 2+ dependent collagenous lectins and plays an important role in innate immunity.
     甘露聚糖结合凝集素 (MBL)是一种肝源性的血清蛋白 ,属Ca2 + 依赖型凝集素家族成员 ,在天然免疫中起重要作用。
短句来源
     MBL(mannan binding lectin),also known as MBP(mannan binding protein),consists of oligomers of identical polypeptide chains.
     甘露聚糖结合凝集素(MBL;或甘露聚糖结合蛋白,MBP)是由相同的多肽链组成的寡聚物,它通过结合细胞表面的碳水化合物能够有效地识别侵入体内的多种致病微生物,并激活补体来杀灭病原微生物。
短句来源
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  “mannan binding lectin”译为未确定词的双语例句
     Mannose binding lectin(MBL),or mannan binding lectin,is a member of C type collectin proteins in the plasma.
     甘露糖结合凝集素(mannose bindinglectin or mannan bindinglectin;MBL)是一种存在于血清中的C型凝集素。
短句来源
     CLONING AND SEQUENCING OF MANNAN BINDING LECTIN cDNA OF CHINESE
     中国人MBLc DNA的克隆与序列分析
短句来源
     A Study on Relativity of Mannose Binding Lectin or Mannan Binding Lectin (MBL) and Acquired Immunodeficiency Syndrome (AIDS)
     甘露糖结合凝集素与艾滋病相关性的研究
短句来源
  相似匹配句对
     Mannan-Binding Lectin and Infectious Diseases
     甘露糖结合凝集素与感染性疾病
短句来源
     Purification and Characterization of Mannan-binding Lectin
     甘露聚糖结合凝集素的分离纯化和活性研究
短句来源
     CLONING AND SEQUENCING OF MANNAN BINDING LECTIN cDNA OF CHINESE
     中国人MBLc DNA的克隆与序列分析
短句来源
     Relationship between the function of mannan-binding lectin and disease
     甘露聚糖结合凝集素的生物学功能及其临床意义
短句来源
     Cloning and characterization of mouse mannan binding lectin-A cDNA
     小鼠MBL-A基因的克隆和序列分析
短句来源
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  mannan binding lectin
Secreted pattern-recognition molecules function as opsonins, the best characterised is the mannan-binding lectin.
      
Heritability estimates for the constitutional levels of the collectins mannan-binding lectin and lung surfactant protein D.
      
Human IgA activates the complement system via the mannan-binding lectin pathway.
      
Genetic variants of the mannan-binding lectin are associated with immune reactivity to mannans in Crohns disease.
      


The intact cDNA encoding the mannan binding lectin polypeptide including the signal sequence was isolated by using RT PCR method with total RNA extracted from fresh liver tissue of a Han nationality foetus, linked into pGEM T vector and transformed into E.coli TG1. The result of sequencing indicated that the sequence of our cDNA is different greatly from that of the reported human MBL cDNA but identical with that of the...

The intact cDNA encoding the mannan binding lectin polypeptide including the signal sequence was isolated by using RT PCR method with total RNA extracted from fresh liver tissue of a Han nationality foetus, linked into pGEM T vector and transformed into E.coli TG1. The result of sequencing indicated that the sequence of our cDNA is different greatly from that of the reported human MBL cDNA but identical with that of the mRNA for human MBL in GenBank except two nuc leotides, and that its encoded product consists of a typical hydrophobic signal sequence of 20 amino acids and a mature MBL polypeptide of 228 amino acids.

从中国汉族人胎肝组织提取总RNA,以RT-PCR方法获取了编码含信号顺序的全长甘露聚糖结合凝集素(MBL)肽链的cDNA片段,将其与pGEM-T载体连接,转化大肠杆菌TG1,建立了MBL的cDNA克隆。序列分析表明:与文献报道的人MBLcDNA序列比较,差异颇大,但与GenBank中的人MBLmRNA比较,仅2个位置的核苷酸不同;其编码产物是20个残基的信号顺序和228个残基的成熟MBL多肽。

Objective To obtain the gene fragment encoding human mannan-binding lectin (MBL)-associated serine protease-2 (MASP-2). Methods The target cDNA fragment was amplified from the total RNA extracted from human fetal liver tissue by RT-nested PCR, cloned into pGEM-T vector and identified by restriction analysis and sequencing. Results The cDNA fragment encoding MASP-2 polypeptide with the signal sequence was isolated, linked with pGEM-T vector and transformed into E.coli TGI. The restriction maps...

Objective To obtain the gene fragment encoding human mannan-binding lectin (MBL)-associated serine protease-2 (MASP-2). Methods The target cDNA fragment was amplified from the total RNA extracted from human fetal liver tissue by RT-nested PCR, cloned into pGEM-T vector and identified by restriction analysis and sequencing. Results The cDNA fragment encoding MASP-2 polypeptide with the signal sequence was isolated, linked with pGEM-T vector and transformed into E.coli TGI. The restriction maps of the selected clones were consistent with those generated by computer analyses. The result of sequencing of one selected clone showed that its sequence was identical with that of reported human MASP-2 cDNA. Conclusion The human MASP-2 gene was successfully cloned, which prepares the ground for studying the molecular mechanisms by which MBL activates the complement lectin pathway and the mutations of MBL gene cause immunodeficiency.

目的 获得人甘露聚糖结合凝集素相关丝氨酸蛋白酶-2(MASP-2)编码区基因。方法采用 RT-巢式 PCR技术从 人胎肝组织总RNA扩增目的cDNA片段,克隆人pGEM-T载体,酶切图谱分析和测序鉴定。结果获得了编码合信号 顺序的MASP-2肽链全长cDNA,将其与pGEM-T载体连接,转化大肠杆菌TG1,建立了MASP-2的重组克隆。酶切图 谱与微机分析结果无异;序列分析表明,与报道的人 MASP-2cDNA序列一致。结论成功克隆了人 MASP-2基因,为深 入研究补体凝集素途径的激活机制和甘露聚糖结合凝集素基因突变引起免疫缺损的发病机制奠定了基础。

Objective To prepare monoclonal antibodies (mAbs) to mannan binding lectin (MBL) and develop a method for detection by using it Methods Mannan binding lectin in human serum was isolated by affinity chromatography and used as antigen to immunize Balb/c mice Three hybridome cell lines that could steadily secret antiMBL mAb were established The mAbs were used for ELISA Results The mAbs specifically recognized MBL molecule with Mr 31 000 and markedly inhibited the effect...

Objective To prepare monoclonal antibodies (mAbs) to mannan binding lectin (MBL) and develop a method for detection by using it Methods Mannan binding lectin in human serum was isolated by affinity chromatography and used as antigen to immunize Balb/c mice Three hybridome cell lines that could steadily secret antiMBL mAb were established The mAbs were used for ELISA Results The mAbs specifically recognized MBL molecule with Mr 31 000 and markedly inhibited the effect of anti complement of MBL mannan complex The MBL levels (2 69±1 58 mg/L) in 186 sera from normal subjects were detected by ELISA Conclusion The monoclonal antibodies to mannan binding lectin were successfully prepared and used for ELISA

目的 制备抗人甘露聚糖结合凝集素单克隆抗体及建立应用方法。方法 用亲和层析法自人血清中提取甘露聚糖结合凝集素 (MBL) ,免疫Balb/c小鼠 ,通过细胞融合的方法 ,得到 3株稳定分泌抗人MBL单克隆抗体 (mAb)的杂交瘤细胞株。建立检测MBL的ELISA。结果 本组mAb识别相对分子质量 (Mr)为 310 0 0的MBL分子 ,与MBL作用后可明显抑制MBL 甘露聚糖复合物的抗补体效应。用ELISA检测 186例健康人血清中MBL水平为 2 6 9± 1 5 8mg/L。结论 成功地获得了抗MBL的mAb并用于建立ELISA。

 
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