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morphine vaccine
相关语句
  吗啡疫苗
     Study on Preparation of Morphine Vaccine and Antibody
     吗啡疫苗及抗体制备的实验研究
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  “morphine vaccine”译为未确定词的双语例句
     Conclusions: The research prepared morphine vaccine and developed an active immunization to prevent morphine from penetrating the blood-brain barrier, and it could offer a new means to block the action of opioids.
     结论:研制吗啡依赖的防治疫苗,采用主动免疫方式免疫动物,诱导机体产生特异性抗体,有效阻止吗啡进入中枢神经系统,从而减弱吗啡的药理作用,降低其成瘾性,此疫苗同时能对抗海洛因成瘾,这将为防止药物滥用寻找新的治疗方向。
短句来源
  相似匹配句对
     Study on Preparation of Morphine Vaccine and Antibody
     吗啡疫苗及抗体制备的实验研究
短句来源
     Preparation and characterization of anti-morphine vaccine antibody
     抗吗啡疫苗抗体的制备及其特性鉴定
短句来源
     influenza vaccine
     流感疫苗
短句来源
     Malaria vaccine
     疟疾疫苗
短句来源
     Morphine Immunoassay
     吗啡的免疫测定
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Aim To investigate the effects of anti-morphine vaccines on pharmacological actions of morphine in mice.Methods Morphine-6-succinyl and morphine were conjugated respectively with Blue Carrier(BC) in the presence of carbodi-imide.After being immunized with both vaccines(M-BC,M-6-S-BC),the withdrawal syndrome and the abirritation were observed in mice.Results The withdrawal syndrome of the immune mice with M-6-S-BC and M-BC vaccines were reduced markedly compared with the model...

Aim To investigate the effects of anti-morphine vaccines on pharmacological actions of morphine in mice.Methods Morphine-6-succinyl and morphine were conjugated respectively with Blue Carrier(BC) in the presence of carbodi-imide.After being immunized with both vaccines(M-BC,M-6-S-BC),the withdrawal syndrome and the abirritation were observed in mice.Results The withdrawal syndrome of the immune mice with M-6-S-BC and M-BC vaccines were reduced markedly compared with the model group,the skipping times were decreased and the latency was longer(P<0.05,P<0.01);The abirritation was decreased markedly compared with that in the model group(P<0.05).Conclusions Both morphine vaccines can elicit a high-titer antibody response and block the morphine to enter the brain and weaken the effects of morphine on CNS.

目的观察两种吗啡疫苗接种小鼠对吗啡药理作用的影响。方法吗啡及吗啡半酯在碳二亚胺催化下分别与B lue Carrier(BC)载体蛋白交联制备吗啡疫苗(M-BC、M-6-S-BC),用其免疫小鼠,观察免疫小鼠对吗啡戒断症状及吗啡镇痛作用的影响。结果与吗啡模型组比较,两种疫苗免疫接种可使吗啡戒断症状减轻,使跳跃潜伏期延长(P<0.05),跳跃次数显著减少(P<0.01);M-BC及M-6-S-BC组的平均扭体次数与吗啡治疗组比较差异有显著性(P<0.05)。结论两组吗啡疫苗均可诱导机体产生特异性抗吗啡抗体,该抗体能够部分中和体内的吗啡,阻止吗啡吸收入脑,缓解吗啡产生的身体依赖性,对抗吗啡的戒断作用及镇痛作用。

AIM: To prepare anti-morphine vaccine antibody with high titer and good specificity and to identify its properties. METHODS: Chemically synthesized 6-succinylmorphine (M-6-S) was cross-linked to BSA in pH 9.0 carbonate buffer. After being purified by gel filtration and (NH_4)_2SO_4 salting out, the immunogen was used to immunize BALB/c mice and SD rats. The titer and specificity of antisera were identified by ELISA and neutralization inhibition test, respectively. The effect of the immunization was examined...

AIM: To prepare anti-morphine vaccine antibody with high titer and good specificity and to identify its properties. METHODS: Chemically synthesized 6-succinylmorphine (M-6-S) was cross-linked to BSA in pH 9.0 carbonate buffer. After being purified by gel filtration and (NH_4)_2SO_4 salting out, the immunogen was used to immunize BALB/c mice and SD rats. The titer and specificity of antisera were identified by ELISA and neutralization inhibition test, respectively. The effect of the immunization was examined by the radiant heat tail-flick (TF) reflex test. RESULTS: The titers of antiserum from immunized BALB/c mice and SD rats were up to 1∶200 000 and over 1∶20 000, respectively. Neutralization inhibition test proved that the anti-morphine vaccine antibody had the cross-reactions to following drugs with analogous structure as morphine: 6-monoacetylmorphine, heroin and codeine. The TF reflex test showed: (1) TF reflexes were notable inhibited after intraperitoneal injection of 2 mg/kg morphine, and (2) percentage of the maximum possible effect (% MPE) reduced 74.7%, (3) the TF latency recovered to the baseline level much more rapidly compared with the control rats. CONCLUSION: The M-6-S-BSA vaccine has been prepared successfully. The BALB/c mice and SD rats immunized with the vaccine can produce anti-morphine antibody with high titer and satisfactory specificity, which may significantly reduce rats’ antinociception against morphine.

目的制备高效价的抗吗啡疫苗的抗体,并对其特性进行鉴定。方法将人工合成的6-琥珀酰吗啡(M-6-S)与BSA在pH9.0碳酸盐缓冲液中交联成M-6-S-BSA。经凝胶柱过滤和硫酸铵法纯化后,用两种不同方案免疫BALB/c小鼠和SD大鼠,用ELISA法和中和抑制试验分别测定抗体的滴度和进行特异性鉴定;用辐射热甩尾(TF)反射试验检测M-6-S-BSA疫苗的免疫效果。结果用合成的M-6-S-BSA疫苗连续免疫小鼠8次,所获抗血清的效价可达1∶200000,大鼠免疫4次后抗血清的效价超过1∶20000。中和抑制试验表明,抗M-6-S抗体对吗啡和与吗啡有共同母环结构的6-单乙酰吗啡、海洛因及可待因具有较高的特异性。免疫大鼠腹腔注射2mg/kg的吗啡后,其甩尾(TF)反射受到明显抑制,最大效应百分率(%MPE)减少了74.7%,TF潜伏期回到基线水平的时间明显快于对照组。结论成功地制备M-6-S-BSA疫苗,用其免疫小鼠和大鼠均可产生高滴度和特异性抗吗啡抗体。该抗体可明显减低大鼠对吗啡的抗伤害感受,为进一步研究吗啡疫苗抗体对抗吗啡的成瘾作用奠定了基础。

OBJECTIVE To investigate the effects of anti-morphine vaccines on morphine concentrations in mice serum and cerebral tissue.METHODS Morphine-6-succinyl prepared by the acid anhydride mixture procedures,and morphine were conjugated with Blue Carrier in the presence of carbodi-imide.After being immunized with both vaccines and freud adjuvant,the morphine concentrations in mice serum and cerebral tissue were determined by radioimmunoassay.RESULTS When the antiserum titers were...

OBJECTIVE To investigate the effects of anti-morphine vaccines on morphine concentrations in mice serum and cerebral tissue.METHODS Morphine-6-succinyl prepared by the acid anhydride mixture procedures,and morphine were conjugated with Blue Carrier in the presence of carbodi-imide.After being immunized with both vaccines and freud adjuvant,the morphine concentrations in mice serum and cerebral tissue were determined by radioimmunoassay.RESULTS When the antiserum titers were over 1∶8 000,the morphine concentration in mice cerebral tissue in M-6-S-BC and M-BC groups were decreased markedly,comared with the model group(P<0.05,P<0.01),and the morphine concentration in mice serum in M-BC group was decreased markedly,compared with the model group((P<0.05)).CONCLUSION Both morphine vaccines elicited a high-titer antibody response,blocked the morphine to enter the brain,and weaken the effects of morphine on CNS.It is indicated that the vaccine may be a promising means to explore new treatments for morphine abuse.

目的观察两种吗啡疫苗对吗啡依赖小鼠脑组织及血液中吗啡含量的影响。方法吗啡及吗啡半酯在碳二亚胺催化下分别与Blue Carrier(BC)载体蛋白交联,用其免疫小鼠,放射免疫分析方法检测免疫小鼠脑组织及血清中吗啡浓度的变化。结果琥珀酰化吗啡酯(M-6-S-BC)组血清中的吗啡浓度与吗啡依赖模型组比较有所增加,但无显著性差异(P>0.05),脑组织中的浓度,明显降低(P<0.01);吗啡酯(M-BC)组血清及脑组织中吗啡浓度与模型组比较差异显著(P<0.05)。结论两种吗啡疫苗都能诱导小鼠产生高滴度的抗吗啡抗体,可产生中和吗啡的作用,阻断吗啡进入大脑,减弱吗啡对中枢神经系统的影响,提示其对控制毒品复吸将有较好的应用前景。

 
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