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   gi gene 的翻译结果: 查询用时:0.203秒
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gi gene
相关语句
  gi基因
     The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.
     用双引物法对GI基因进行体外定点突变,构建了GI突变体G138P。
短句来源
     2. gE/gI gene The PRV D2 strain, absence of gE/gI gene, was sensitive to Vero、 IBRS-2、 Marc-145、 MDBK and ST cells. Especially obvious CPE appeared in MDBK mono-cells post infection with the PRV D2 strain during 8-48h.
     2.gE/gI基因 gE/gI基因缺失毒株D2对上述5种细胞均较敏感,其在细胞增殖并引起细胞病变之能力均不如PRV Fa株明显。
短句来源
     The mutants of Q20L and G247D of glucose isomerase (GI) were constructed by in vitro site directed mutagenesis of GI gene with double primersmethod.
     用双引物法对GI基因进行体外定点突变 ,构建了突变体Q2 0L和G2 47D。
短句来源
     The universal transfer vector pgD-M-gE deleted the gI gene and 363 bp in the 5' end of the gE ORF of PRV.There were 11 restrication sites for insertion of the foreign gene. The upstream and downstream flanking sequences were up to 1.25 kb and 1.42 kb.
     该转移载体缺失了伪狂犬病病毒gI基因和gE基因ORF5'端363bp的碱基,有11个酶切位点可供外源基因的插入,上下游侧翼分别为1.25bp和1.42bp。
短句来源
     Cloning and Sequencing Analysis of gE and gI Gene of Pseudorabies Virus SH Strain
     伪狂犬病病毒上海株gE和gI基因的克隆及序列分析
短句来源
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  ge基因
     The universal transfer vector pgD-M-gE deleted the gI gene and 363 bp in the 5' end of the gE ORF of PRV.There were 11 restrication sites for insertion of the foreign gene. The upstream and downstream flanking sequences were up to 1.25 kb and 1.42 kb.
     该转移载体缺失了伪狂犬病病毒gI基因和gE基因ORF5'端363bp的碱基,有11个酶切位点可供外源基因的插入,上下游侧翼分别为1.25bp和1.42bp。
短句来源
     Blasted with gE and gI gene of PRV FA from GenBank,2 bases in gE were found to have point mutation,and the homology was over 99;
     结果发现,PRVFB和PRVFA株的gE基因有2处碱基发生了点突变,同源性为99%;
短句来源
     According to the Genbank open sequence,two pairs of primer were designed in order to amplify gE gene and partial gI gene of pseudorabies virus Shanghai strain,respectively. The gE gene and partial gI gene were obtained by polymerase chain reaction(PCR) ,subsequently cloned into pGEM-T vector. Then both of them were inserted into pUC18 vector.
     根据已发表的伪狂犬病病毒 (PseudorabiesVirus,PRV)的 gI和 gE 基因序列 ,设计两对引物用PCR方法扩增出PRV SH gI和 gE 基因 ,将其克隆入 pUC18载体中 ,获得了缺失部分gI基因的转移载体质粒 ,命名为 pgEI。
短句来源
     On the basis of cloning and indentifying gE gI gene of pseudurabie virus SH strain,both of gE gene and gI gene were cloned into pUC 18,resulting in a recombinant pgEI. The GFP expressing cassettle and Lac Z genes were inserted into the Bam H Ⅰ Bst p Ⅰ deleted locus of gE 5′terminal,produced recombinant pgEI GFPZ. BHK 21 cell which was infected with PRV SH was transfected with the complex of pgEI GFPZ and DOTAP.
     在伪狂犬病病毒 (PRV)上海株gI和gE基因克隆鉴定的基础上 ,用BamHⅠ +BstpⅠ去掉gE基因的 5′端 36 3bp ,在缺失位置插入绿色荧光蛋白 (GFP)和LacZ基因 ,构建含双报告基因的PRV SH转移载体pgEI GFPZ。
短句来源
     At first,gE gene and gI gene were cloned into pUC18,constructed the pgEI vector. Then, the 5'trminal sequence of gE gene was deleted 363bp using the restrict endonuclease in gE gene,The GFP expressing cassette was inserted into the deleting site.
     然后用限制性内切酶BamHI和BstpI缺失掉gE基因 5’端 363bp,同时把绿色荧光蛋白 (GFP)基因表达盒插入到缺失部分 ,并在下游引入一个多克隆位点 ,构建了缺失转移载体pgEI GFP。
短句来源
  “gi gene”译为未确定词的双语例句
     The recombinant FPV expressing gI gene of MDV was named rFPV-MDV648gI.
     结果表明接种FPv一MDv648gl后鸡血清中含有抗gI抗体。
短句来源
     According to gI sequences of MDV 648A strain, two primers were designed, and MDV gI gene was amplified by PCR.
     根据MDV 648A株病毒基因组gl基因序列,我们设计了两个引物,用PCR扩增出MDVgl基因。
短句来源
     Plasmid PPB7 is a recombinant of plasmid pBR322 and the BamHI 1 7 fragment of Pseudorabies Virus (PRV), from which the recombinant plasmid PPB7 1 deleted gI gene and part of gP63 gene were obtained by way of digestion with restriction endonucleotidase NcoI.
     用限制性核酸内切酶NcoI消化含有伪狂犬病病毒(PRV) Fa株Bam HI-7片段的质粒PBB7,以低融点琼脂糖回收目的片段, 经连接并转化E. coliDH5a, 获得缺失了gI和部分gp63基因的重组质粒PPB7-1。
短句来源
     The universal transfer vector pgD-M-gE has deleted the gI gene and 363bp in the 5'end of the gE ORF of PRV.There were 11 restrication sites for insertion of the foreign gene. The upstream and downstream flanking sequences were up to 1.25kb and 1.42kb.
     该转移载体缺失了伪狂犬病病毒 PI基因和 PE基因 ORF 5’端 363hP的碱基,有 11个酶切位点可供外源基因的插入,上下游侧翼分别为 1.25kb和 1.42kb。
短句来源
     Replacement plasmid for homologous recombination was also constructed by inserting tsr gene into glucose isomerase gene. The homologous recombination of GI gene in M1033 chromosomes was achieved by using denatured linearized DNA fragments and glucose isomerase deficient strain M1033LJ was obtained.
     构建了葡萄糖异构酶(GI)结构基因内插入硫链丝菌肽基因(tsr)的置换型同源重组质粒,利用其变性双链片段实现与M1033菌株染色体上基因的同源重组,获得置换型葡萄糖异构酶缺陷型菌株M1033LJ。
短句来源
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  gi gene
The results revealed that the gI gene is encoded by a 1.6-kb transcript which is 3' coterminal with a 3.0-kb gD mRNA while the gE gene is encoded by two transcripts of 3.5- and 2.4-kb in size.
      
This study provides evidence that down-regulation of the GI gene by co-suppression could delay bolting in a cold-sensitive long-day (LD) plant.
      
Expression of the GI gene in T1 plants was much reduced compared to both wildtype plants and plants transformed with pCAMBIA3301 (positive control).
      
Twenty-five plants were dipped into a suspension of Agrobacterium carrying a 2.5?kb antisense GI gene fragment from Arabidopsis, along with the gusA and bar reporter genes, all under the control of a CaMV 35S promoter.
      
A late-flowering transgenic radish has been produced by the expression of an antisense GIGANTEA (GI) gene fragment using a floral-dip method.
      
更多          


オlasmid PPB7 is a recombinant of plasmid pBR322 and the BamH I7 fragment of Pseudorabies Virus(PRV).The recombinant plasmid PPB71 deleted gI gene and part of gp63 gene were obtained from it by way of digestion with restriction endonucleotidase Nco I.By cotransfection of PRV Fa DNA and PPB71 DNA,a deletion mutant,designated PFDI/D63,was constructed with calcium phosphate transfection system.Inoculation of mice with 2.0×107 PFU of the recombinant viruses revealed that mice were partly protected against challenge with PRV Fa containing 2 MLD....

オlasmid PPB7 is a recombinant of plasmid pBR322 and the BamH I7 fragment of Pseudorabies Virus(PRV).The recombinant plasmid PPB71 deleted gI gene and part of gp63 gene were obtained from it by way of digestion with restriction endonucleotidase Nco I.By cotransfection of PRV Fa DNA and PPB71 DNA,a deletion mutant,designated PFDI/D63,was constructed with calcium phosphate transfection system.Inoculation of mice with 2.0×107 PFU of the recombinant viruses revealed that mice were partly protected against challenge with PRV Fa containing 2 MLD.

用限制性核酸内切酶NcoI消化含有伪狂犬病病毒(PRV)Fa侏BamHI-7片段的质粒PPB7,以低融点琼脂糖回收目的片段,经连接并转化E.coliDH5d,获得缺失3gI和部分gp63基因的重组质粒PPB7-1。将PRVFa与PPB7-1DNA共同转染PK15单层细胞,待出现50%以上细胞病变时收获病毒,并以蚀斑法得到纯化重组病毒株,命名为PFDI/D63。小鼠试验证实缺失株对小鼠具有一定的免疫原性。

In order to enhance the thermostability of D glucose isomerase(GI), Gly 138 was decided to be the target to be replaced by molecular design. The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene. The recombinant plasmid pTKD GI containing mutant site was expressed in E.coli K38 strain. The comparison experiments of GIG138P with wild type GI showed that: (1)The half time of GIG138P was as about two times as that of the wild type. (2)The optimum...

In order to enhance the thermostability of D glucose isomerase(GI), Gly 138 was decided to be the target to be replaced by molecular design. The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene. The recombinant plasmid pTKD GI containing mutant site was expressed in E.coli K38 strain. The comparison experiments of GIG138P with wild type GI showed that: (1)The half time of GIG138P was as about two times as that of the wild type. (2)The optimum temperature of GIG138P was increased by 10~12 ℃. (3)The specific activity of GIG138P was similar to the wild type GI. We suppose, based on the above facts, that the substitution of Pro for Gly at position 138 introduced a pyrrolidine ring, which could just fill perfectly the empty hole leaved by Gly 138 which has no side chain and could make the protein structure more rigid, therefore the mutant G138P enhanced the thermostability of SM33GI.

通过分子设计,确定Gly138为改善葡萄糖异构酶(GI)热稳定性的目标氨基酸。用双引物法对GI基因进行体外定点突变,构建了GI突变体G138P。含突变体的重组质粒pTKD-GIG138P在E.coliK38菌株中表达。GIG138P与野生型GI比较实验表明:(1)GIG138P的热失活半衰期约是野生型GI的2倍;(2)GIG138P的最适反应温度提高了10~12°C;(3)GIG138P的比活与野生型GI相当。初步分析认为,Pro取代138位的Gly后,可能由于引入了一个吡咯烷环,该侧链刚好能够充填由于Gly138无侧链基团而留下的空洞,使蛋白质空间结构更具刚性,从而提高了酶的热稳定性。

Plasmid PPB7 is a recombinant of plasmid pBR322 and the BamHI 1 7 fragment of Pseudorabies Virus (PRV), from which the recombinant plasmid PPB7 1 deleted gI gene and part of gP63 gene were obtained by way of digestion with restriction endonucleotidase NcoI. By cotransfection of PRV Fa DNA and PPB7 1 DNA, a deletion mutant, designated PFDI/D63, was constructed with calcium phosphate transfection system. Inoculation of mice with 2.0×10 7PFU of the recombinant viruses revealed that mice were partly...

Plasmid PPB7 is a recombinant of plasmid pBR322 and the BamHI 1 7 fragment of Pseudorabies Virus (PRV), from which the recombinant plasmid PPB7 1 deleted gI gene and part of gP63 gene were obtained by way of digestion with restriction endonucleotidase NcoI. By cotransfection of PRV Fa DNA and PPB7 1 DNA, a deletion mutant, designated PFDI/D63, was constructed with calcium phosphate transfection system. Inoculation of mice with 2.0×10 7PFU of the recombinant viruses revealed that mice were partly phylactic against PRV Fa containing 2 MLD.

用限制性核酸内切酶NcoI消化含有伪狂犬病病毒(PRV) Fa株Bam HI-7片段的质粒PBB7,以低融点琼脂糖回收目的片段, 经连接并转化E.coliDH5a, 获得缺失了gI和部分gp63基因的重组质粒PPB7-1。将PRVFa与PPB7-1DNA共同转染PK15单层细胞,待出现50% 以上细胞病变时收获病毒, 并以蚀斑法得到纯化重组病毒株, 命名为PFDI/D63。小鼠试验证实, 缺失株对小鼠具有一定的免疫原性。

 
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