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mice embryo
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  小鼠胚胎
     Cryopreservation of MMTV-Wnt-1 Transgenic Mice Embryo by Using High Concentration Ethylene Glycol
     利用高浓度乙二醇冷冻保存MMTV-Wnt-1转基因小鼠胚胎
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     Objective To study the relationship between the quantities of epidermal growth factor (EGF), transforming growth factor β 1 (TGF β 1), and insulin like growth factor Ⅱ (IGF Ⅱ ) and the quality of embryo during the culture of mice embryo.
     目的 研究小鼠胚胎培养过程中分泌表皮生长因子 (Epidermalgrowthfactor ,EGF)、转化生长因子 β1(Transforminggrowthfactor β1,TGF β1)、胰岛素样生长因子 Ⅱ (Insulin likegrowthfactor Ⅱ ,IGF Ⅱ )的量与胚胎质量的关系。
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     Objective To investigate the potential influence of zinc deficiency and excessiveness on the differentiation and cytotoxicity to mice embryo limb bud cells.
     目的观察锌缺乏与锌过量对小鼠胚胎肢芽细胞分化的影响及其毒性作用。
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     Objective To determinate the damage of DNA,the degree of DNA damage and the dose-effect of mice embryo fibroblast (3T6) caused by DEHP us ing single cell gel electrophoresis (SCGE).
     目的 应用单细胞凝胶电泳 (SCGE)技术对增塑剂 (DEHP)邻苯二甲酸二乙基己酯致小鼠胚胎成纤维细胞 (3T6细胞 )DNA损伤、损伤程度以及有无剂量 -效应关系进行检测。
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     Adopting one step and two\|step method,we use Lats knock\|out mice embryo to optimize the condition of vitrified with straw and cell freezing tube. The developing percentage of thawed embryo reached 90%,90.1%,89.2% and 91.4%,respectively.
     用冷冻麦管、细胞冷冻管分别采用一步法和二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻 ,胚胎解冻后 ,囊胚发育率分别为 90 %、90 1%、89 2 %和 91 4 % ;
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  “mice embryo”译为未确定词的双语例句
     Expression of angiopoietin-1/-2 in the process of mice embryo implantation.
     促血管生成素-1,-2基因在小鼠着床期子宫内膜的表达
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     Methods Using the mice embryo fibroblast not treated with DEHP as the negative control group,80? μmol/L H\-2O\-2 treated mice embryo fib ro blast as the positive control group,the different concentration DEHP exposed to mic e embryo fibroblast was as four dose groups (62.5,125.0,250.0,500.0??μg/ml),D NA damage was detected by SCGE.
     方法 将溶剂二甲基亚砜处理的 3T6细胞作为阴性对照组 ,用H2 O2 染毒的 3T6细胞作为阳性对照组 (80 μmol/LH2 O2 染毒 ) ,将不同浓度DEHP处理的 3T6细胞设为 4个剂量组 (6 2 5 ,12 5 ,2 5 0 ,5 0 0 μg/ml)进行SCGE检验。
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     Objective To construct an oral DNA vaccine pcDNA3.1+/flk-1_(n1-7) against tumor angiogenesis and investigate the effects and mechanism of the vaccine on tumor development and metastasis in vivo. Methods 1.Total RNA was extracted from BALB/c mice embryo. Extracellular domain of flk-1 was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR).
     目的:制备抗肿瘤血管生成口服DNA疫苗pcDNA3.1+/flk-1_(n1-7),研究该疫苗抗BALB/c小鼠大肠癌生长和转移的作用,并探讨其可能的作用机制。
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     Deformed Effect of Bepridil on Mice Embryo
     苄普地尔对小鼠的致畸作用
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     Objective: To study the effect of all-trans retinoic acid (ATRA) on the expression of TGF-β_1 protein in mouse blastocysts in vitro and its possible roles on the preimplantation mice embryo development.
     目的:探讨全反式维生素A酸(ATRA)对体外培养小鼠囊胚转化生长因子β1 (TGF β1 )蛋白表达的作用,并了解其对着床前期胚胎发育的影响作用。
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  相似匹配句对
     Progress on Cryopreservation of Mice Embryo
     小鼠胚胎冷冻保存技术研究进展
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     DEFORMING EFFECT OF BEPRIDIL ON MICE EMBRYO
     苄普地尔对小鼠的致畸作用
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     The Cat and the Mice
     猫和老鼠
短句来源
     Influence of S L K on C N S of Mice
     神乐康对中枢神经系统功能的影响
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     EMBRYO TRANSFER IN PIGS
     猪的胚胎移植
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  mice embryo
Teratogenicity of oral chaetochromin, a polyphenolic mycotoxin produced byChaetomium spp., to mice embryo
      
Single-strand DNA breaks have been reported following exposure of cultured mice embryo cells and Chinese hamster ovary cells to mercuric chloride.
      
For the third study, 10 mice embryo had a sham surgery to the trachea 3 h before the sampling of lung.
      
Defect in hematopoietic colony formation by fetal liver cells from Fli1 mutant mice Embryo no.
      


Activities of protein disulfide reductase were first shown by Nickerson and Falcone in yeast and later on by Hatch and Turner in germinating seeds of peas. Similar enzyme activities have been observed in mammalian tissues in our preliminary studies.In the present paper some results on the partial purification and properties of protein disulfide reductase in regenerating rat liver are reported.The enzyme activity was determined by measuring the increased amount of protein-SH groups formed from a protein fraction...

Activities of protein disulfide reductase were first shown by Nickerson and Falcone in yeast and later on by Hatch and Turner in germinating seeds of peas. Similar enzyme activities have been observed in mammalian tissues in our preliminary studies.In the present paper some results on the partial purification and properties of protein disulfide reductase in regenerating rat liver are reported.The enzyme activity was determined by measuring the increased amount of protein-SH groups formed from a protein fraction prepared from rat liver used as substrate.Protein-SH groups were estimated by a modified amperometric titration technique.By means of ammonium sulfate fractionation, followed by DEAE-cellulose adsorption and eluation, the enzyme was purified approximately 200 fold and separated completely from the activity of glutathione reductase. The enzyme preparation requires NADPH or NADH for its activity. The optimum pH is about 7.5. It is strongly inhibited by Zn~(++), Cu~(++), Hg~(++), iodoacetic acid and p-chloromercuribenzoate in a final concentration of 10~(-5)M. Reduced glutathione has no stimulatory effect on the enzyme activity. Besides oxidized glutathione, the purified enzyme preparation is also inactive towards some other disulfide compounds of low molecular weight such as lipoic acid and cystine. It is also inactive towards some crystalline proteins such as insulin, ribonuclease, bovin serum albumin and lysozyme. However, a low activity was noticed with bovin serum albumin as substrate when a boiled extract of the liver protein was added.Higher enzyme activities were found in some proliferating tissues such as regenerating liver, liver tumor and mouse embryo.

本文报告在大鼠组织中存在有促使肝蛋白—S—S—还原为蛋白—SH的酶活力,而以再生肝、肝癌、小鼠胚胎等组织的活力为高。利用硫酸铵分步沉淀及DEAE-纤维素吸附等法,可将再生肝中酶活力提纯约200倍,并可与谷胱甘肽还原酶活力完全分开。酶活力需要NADPH或NADH作为供氢体。最适pH为7.5。受Cu~(++)、Zn~(++)、Hg~(++)、对氯汞苯甲酸、一代碘乙酸等物质抑制。谷胱甘肽对酶活力无激活作用。提纯酶制剂不含有还原小分子—S—S—化合物的活力,对一些含—S—S—链的结晶蛋白质也无活力。

The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The...

The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The Golgi apparatus appeared to be horse-shoe shaped in the apical ectodermalthickening and S-shaped in the neighboring ectoderm. It consisted of small vesicles andtubules without any large vesicle. The mitochondria in the neighboring ectoderm were usually round or oval in shape,with fewer and irregular cristae and lighter matrix. The mitochondria of the mesenchy-mal cells were even more embryonic in structure with only 2--3 oblique cristae and alarge inner chamber. The mitochondria in the supperficial layers of the apical ectoder-mal thickening were more or less similar to those in the neighboring ectoderm. Rod-shaped and filamentous mitochondria increased in number in the deeper layers of theapical thickening. Such filamentous mitochondria had vertical cristae, denser matrix andoval and light inner chambers. Transitional stages between the very small vesicles withvague cristae and the large mitochondria with distinct cristae were found in our leadstained preparations. Except in the Golgi region, ribosomes grouped into ring or tubular forms weredistributed all over the cytoplasm in the three kinds of the embryonic cells studied.Transitional stages between such ribosome clusters and the granulated vesicles and tubulescould be clearly demonstrated in the lead stained specimens. In the apical ectodermal thickening, granulated endoplasmic reticulum in the formof scattered small vesicles and tubules seemed to be more abundant than in the neighbor-ing ectoderm and underlying mesenchymal cells. But the most striking difference foundin the apical thickening was the expansion of the granulated endoplasmic reticula intolarge saccules, the cisternae of which containing a grayish dense substance. Another interesting phenomenon found in the apical ectodermal thickening was thepresence of dense bodies in the cytoplasm. They were in various sizes and densitieswith dense granules, masses or cords and various forms of vesicles. They might be round,oval or irregular with a complete, partial or entire absence of limiting membrane. Thosewithout membrane were usually irregular in form and could hardly be demarkated from their surrounding cytoplasm whence assembly of ribosome clusters, mitochondria andendoplasmic reticulum from the surrounding cytoplasm to form the dense bodies could befound. Preliminary histochemical studies found them to be positive in alkaline and acid-phosphatases and RNA staining. They were therefore considered to be lysosomes orcytosomes. The significance of the differences in mitochondrial form and structure, abundantand expanded cisternae of the granulated endoplasmic reticulum and the presence of thedense bodies in the apical ectodermal thickening in relation to embryonic differentiationof the limb was discussed.

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的...

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的动貭有独特形态,即池的一端扩张成大泡,内含网状致密物,显然含有较多的,要不是特异的蛋白貭。这种扩张动貭对于胚胎分化及诱导的关系曾加讨论。 5.动质的形成有证据表明是先从核朊粒群落出现膜成为动貭小泡或小管,再并合成较大的小泡和较长的小管。 6.增厚外胚层细胞的另一特点是比较普逼的存在着形状、大小及密度不同的致密体。有的完全致密,有的泡状,有的是二者的混合体,有的界限清楚,有的处于分散状态。在形态上类似溶酶体和卵子的多泡体。内含类似核朊粒的致密粒、退化线粒体及动质膜。这些致密体和细胞膜、核膜及高尔基体未显有何关系。基膜及细胞膜完好。未见增厚外胚层细胞有排出、吞食或饮液现象,亦未见致密体排至中胚层,故认为这些致密体起于胞貭。对于分化诱导关系曾提出讨论。 7.细胞核及核膜在三种细胞未见有显著不同。核膜有孔,其外膜可与动貭膜相连,核仁小,紧靠核膜,未见有排出现象。氢氧化铅染色的标本有时显示染色貭有微丝,这在胞貭核朊粒群落亦可出现。 8.细胞膜完整,比较直,膜的内侧有一层致密物貭,无桥粒。

The apical ectodermal thickening of the limb buds of mouse embryos, 11-day vaginalplug age, and epithelial cells of the villi of small intestine, 19-day mouse fetus, wereused for this observation. The limb bud ectoderm was fixed in 1% osmic acid andstained in 1% Pb(OH)_3, while the small intestine was fixed in 1.2% potassium per-magnate. There were transitional stages from minute dense vesicles of 0.01--0.07μ with afew vague striations, gradually growing up into vesicles of 0.07--0.10μ with definitemitochondrial...

The apical ectodermal thickening of the limb buds of mouse embryos, 11-day vaginalplug age, and epithelial cells of the villi of small intestine, 19-day mouse fetus, wereused for this observation. The limb bud ectoderm was fixed in 1% osmic acid andstained in 1% Pb(OH)_3, while the small intestine was fixed in 1.2% potassium per-magnate. There were transitional stages from minute dense vesicles of 0.01--0.07μ with afew vague striations, gradually growing up into vesicles of 0.07--0.10μ with definitemitochondrial cristae, and finally into organelles that could be definitely identified assmall mitochondria of 0.13×0.20μ in size, They were neither fragments of degenera-ting mitochondria nor lysosomes, since their structures became more and more similar tothat of the mitochondria with their increase in size. These minute dense vesicles werefound among clusters of ribosomes, at first vaguely outlined and then with definite mem-branes and dense substance. The significance of ribosomes in relation to synthesis of themembranous protein and the matrix of the mitochondria was discussed. In the course of the formation of granulated endoplasmic reticulum, diffuse cyto-plasmic ribosomes at first arranged themselves into circular or tubular clusters. Theseclusters then transformed themselves into membrane bounded tubules and vesicles. Byfurther fusion and extension, definite granulated endoplasmic reticula were formed.Close relationship between mitochondrium, granulated endoplasmic reticulum and Golgibody has been observed but there showed no evidence of developmental significance.The granulated endoplasmic reticulum was thus considered to be formed by ribosomes.

在11天小白鼠胚胎肢芽尖端增厚外胚层细胞,经过1%锇酸Veronal缓冲液,pH7.6,固定,1%氢氧化铅染色的电子显微镜照相中显示在细胞貭基貭中,在核朊粒群落之间出现0.013—0.07μ的致密微体,中有纵纹,过渡到0.07—0.1μ的卵圆微体时已明显的呈綫粒体结构,中有嵴数条,长大0.13—0.20μ时和这一时期这种细胞的綫粒体完全相同了。用1.2%过锰酸钾固定的小白鼠19天胚胎的小肠绒毛上皮,有0.09μ的小泡过渡到显然是綫粒体结构,0.3—0.5μ的小体。成长的绒毛上皮綫粒体直径约为1μ。动貭的形成过程是弥散的核朊粒排成环状或双行的群落,就地出现膜而成有粒小泡和有粒小管,再扩大合并成为有粒内貭网或动貭。

 
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