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liposome complex
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  脂质体复合物
     Wild-type CDKN2 cDNA-Stearylamine(SA) cationic liposome complex was transfected into NSCLC cell line A549 cells and SCLC cell line SH77 cells, The proliferation of the cells was detected by MTT methods to examine whether CDKN2 gene inhibited the proliferation of non-small cell lung cancer (NSCLC) cell.
     转染野生型CDKN2cDNA—SA脂质体复合物到NSCLC细胞系A549和SCLC细胞系SH77,用MTT方法测量细胞增殖活力,观察CDKN2基因是否能抑制非小细胞肺癌细胞的增殖。
短句来源
     Distribution and Safety Studies of IBV DNA Vaccine and DNA/Cationic Liposome Complex
     IBV DNA疫苗、DNA疫苗—脂质体复合物在鸡体内的分布及安全性研究
短句来源
     Experimental study of transfection by HBV DNA vaccine cationic liposome complex in mice
     HBV DNA疫苗阳离子脂质体复合物转染小鼠实验研究
短句来源
     Preparation and Transfection of GFP Plasmid DNA/Cationic Liposome Complex
     GFP质粒DNA/阳离子脂质体复合物的制备及其体外表达
短句来源
     Voltammetric and spectrometric investigation of induced DNA release from liposome complex
     循环伏安法和光谱法检测阴离子磷脂诱导DNA从脂质体复合物中的释放
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  “liposome complex”译为未确定词的双语例句
     This research provided scientific datas for the applying of IBV DNA vaccine and DNA/Catiomc liposome complex.
     本研究为IBV DNA疫苗及脂质体在DNA疫苗中的应用提供了科学依据。
短句来源
     Methods One pair of Survivin target sequence-specific small interfering RNA(siRNA)was designed and synthesized,then siRNA/liposome complex was used to transfect bladder cancer cell line-T24 with increasing concentra- tions(50-200 nmol/L).
     方法设计、合成一对Survivin编码基因序列特异的小分子干扰RNA(siR- NA),用脂质体包裹转染T24膀胱癌细胞,分不同的浓度组(50~200 nmol/L)。
短句来源
     METHODS Plasmid pEGFP-N1 liposome complex was introduced into the murine ES cells and the transfected clones were filtered in the presence of G418. Strong fluorescent GFP clones were singly picked out and further proliferated.
     方法质粒pEGFP-N1脂质体复合体转染小鼠胚胎干细胞,经G418筛选后选取GFP强阳性克隆进行扩增建系。
短句来源
     MethodsSMSCs were transfered by pAGFP rhBMP 2 liposome complex.
     方法 pEGFP rhBMP 2的扩增、浓度及纯度鉴定。
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     Test with hypophysectomized rats showed that a extended releasing effect of 3 or 4 days was reached by the pGH/liposome complex.
     去垂体大鼠的实验结果表明 ,pGH/脂质体可在 3~ 4d内发挥良好的缓释作用
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  相似匹配句对
     Liposome complex and genotherapy for tumours
     脂质体复合物与肿瘤的基因治疗
短句来源
     MethodsSMSCs were transfered by pAGFP rhBMP 2 liposome complex.
     方法 pEGFP rhBMP 2的扩增、浓度及纯度鉴定。
短句来源
     On Complex Reality
     复杂的实在
短句来源
     Complex object
     复合宾语
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     Liposome and its application
     脂质体及其应用
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  liposome complex
When βAP (25-35) peptide was mixed with the pDNA/liposome complex and used, the complexes lost their ability to transfect.
      
Further studies with a?P-channels incorporated into planar lipid bilayers from the liposome complex have also revealed that the channel activity can express spontaneous transitions to a much higher range of conductances between 400 and 4000 pS.
      
The particle size of the liposome complex-encapsulated K6H2[CoW11TiO40], is distributed in two uncontinuous limits in which 98.9% of particles distributed in the limit of 62.8-76.7 nm, average diameter, is 69.4 nm.
      
The nanosize liposome complex-encapsulated polyoxotungstate K6H2[CoW11TiO40] has been prepared and structurally characterized by i.r., u.v.-vis and e.s.r.
      
Preparation, characterization and in vitro antitumoral activity of a nanosize liposome complex encapsulated polyoxotungstate K6H
      
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Exogenous recombinant expression plasmid of sense p53 cDNA, antisenes p53 cDNA and the expression vector pREP9 were directly transferred into colon adenocarcinoma cells (SW1116) in nude mice respectively by injectng DNA-liposome complex into the tumor body, while control group was injected with tormal saline. Then, the streptin-biotin-peroxidase complex method was performed to detect the expression of p53 gene immunohistochemically in the transfected cells by using the anti-mutant p53 protein as...

Exogenous recombinant expression plasmid of sense p53 cDNA, antisenes p53 cDNA and the expression vector pREP9 were directly transferred into colon adenocarcinoma cells (SW1116) in nude mice respectively by injectng DNA-liposome complex into the tumor body, while control group was injected with tormal saline. Then, the streptin-biotin-peroxidase complex method was performed to detect the expression of p53 gene immunohistochemically in the transfected cells by using the anti-mutant p53 protein as the first antibody. The results demonstrated that half of SW116 cells cultured in vitro contained mutant p53 protein. And positive rates of mutant p53 protein on the sections of transplanted tumor in nude mice treated with antisense p53 cDNA expression plasmid transfectiom,normal saline injection,pREP9.sense p53 cDNA plasmid transfection were 25%,66. 7%,77. 8%,66.7% respectively. There existed a significant difference between the first group and others, and no difference among the latter three groups. While no significant difference was found between the tumor sizes among these four groups. So,a conclusion was drown that expression of exogenous antisense p53 gene could block the synthesis of mutant p53 protein in SW1116 cells.

采用直接注射外源DNA-脂质体复合物的方法分别在荷瘤裸鼠人肠癌组织中转染外源正义p53cDNA和反义p53cDNA的重组表达质粒及非整合型哺乳动物表达载体pREP9,并以注射等量生理盐水为对照,然后应用链菌素亲生物蛋白-过氧化酶免疫组化法检测肿瘤组织中突变型p53蛋白的表达。结果表明:体外培养SW1116细胞中约有50%为突变型p53蛋白阳性;转染反义p53cDNA表达质粒组的裸鼠移植瘤细胞p53蛋白阳性率为25%,而注射生理盐水、转染pREP9及正义p53cDNA表达质粒组阳性率分别为66.7%,77.8%,66.7%;前者与后三者之间有显著住差异(P<0.01,X[2]检验),但各组间肿瘤大小并无显著性差异。据此认为,肿瘤体内直接转染外源反义p53基因能封闭肠癌SW1116细胞中突变型p53基因的表达。

Wild-type CDKN2 cDNA-Stearylamine(SA) cationic liposome complex was transfected into NSCLC cell line A549 cells and SCLC cell line SH77 cells, The proliferation of the cells was detected by MTT methods to examine whether CDKN2 gene inhibited the proliferation of non-small cell lung cancer (NSCLC) cell. Results showde: on days 1, 3, 5, 8 after CDKN2 gene transfection the OD values of A549 cells were obviously lower than those of control groups without CDKN2 gene transfection (P<0. 01 ), while the OD values...

Wild-type CDKN2 cDNA-Stearylamine(SA) cationic liposome complex was transfected into NSCLC cell line A549 cells and SCLC cell line SH77 cells, The proliferation of the cells was detected by MTT methods to examine whether CDKN2 gene inhibited the proliferation of non-small cell lung cancer (NSCLC) cell. Results showde: on days 1, 3, 5, 8 after CDKN2 gene transfection the OD values of A549 cells were obviously lower than those of control groups without CDKN2 gene transfection (P<0. 01 ), while the OD values of SH77 cells showed no difference compared with the control groups (P>0. 05 ). CDKN2 gene could significantly inhibit the proliferation of NSCLC cells and had little effect on SCLC cells in vitro. The observations suggest that the tumor suppressor gene CDKN2 is a potential candidate for ge ne replacement therapy of NSCLC.

转染野生型CDKN2cDNA—SA脂质体复合物到NSCLC细胞系A549和SCLC细胞系SH77,用MTT方法测量细胞增殖活力,观察CDKN2基因是否能抑制非小细胞肺癌细胞的增殖。结果:SA阳离子脂质体介导CDKN2基因转染A549细胞后第1、3、5、8日OD值显著小于对照组(P<0.01),而SH77细胞转染CDKN2基因后1、3、5、8日OD值与对照组比较无差别(P>0.05)。在体外转染CDKN2基因能显著抑制NSCLC细胞增殖,对SCLC细胞无作用。结果表明肿瘤抑制基因CDKN2可望成为NSCLC基因替代治疗的候选基因。

An eukaryotic expression plasmid Rc/CMV GHcDNA containing CMV late promoter and hGH cDNA was constructed and introduced into mouse skeletal muscle by means of direct injection with or without the cationic liposome (Lipofectin and Lipofectamine reagent) intramuscularly. The expression of the gene was detected through both RT-PCR and IRMA (Immunoradiometric assay) at the level of transcription and protein respectively. The gene expression can be detected even after 90 days of single plasmid injection. Compared...

An eukaryotic expression plasmid Rc/CMV GHcDNA containing CMV late promoter and hGH cDNA was constructed and introduced into mouse skeletal muscle by means of direct injection with or without the cationic liposome (Lipofectin and Lipofectamine reagent) intramuscularly. The expression of the gene was detected through both RT-PCR and IRMA (Immunoradiometric assay) at the level of transcription and protein respectively. The gene expression can be detected even after 90 days of single plasmid injection. Compared with the mice injected with the plasmid itself, the expression levels of mice injected with the plasmid-cationic liposome complex seem to be higher, and the expression period longer. Furthermore, the effect of Lipofectamine seems to be even better than that of Lipofectin. The results indicate that direct intramuscular injection with recombinant expression plasmid or plasmid-cationic liposome complex is a simple, efficient and economical way for foreign gene transfer and expression in experimental mice in vivo.

本文以构建的含人生长激素(hGH)cDNA的重组真核表达质粒为外源靶基因,研究了直接注射质粒及脂质体与质粒复合物后hGH在小鼠体内表达的情况.用RT-PCR技术检测hGH转录产物,用免疫放射检测分析法(IRMA)检测hGH蛋白质表达水平.结果发现,注射质粒与脂质体复合物组的表达效率高于单纯质粒组,Lipofectamine的作用强于Lipofectin,表明应用脂质体及直接注射法是使外源靶基因在小鼠体内表达的简便、有效途径.

 
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