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p蛋白
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  p 24 protein
     SDS PAGE proved that 46% of recombinant p24 protein was expressed in BL21(DE3)plysS.
     SDS PAGE分析表明 ,受体菌BL2 1(DE3)plysS表达量最高 ,表达的重组p2 4蛋白占菌体总蛋白的 46 %。
短句来源
     High Level Soluble Expressiion, One Step Purification and Characterization of HIV-1 p24 Protein
     HIV-1p24蛋白在大肠肝菌中的高效可溶性表达、一步纯化及抗原性分析
短句来源
     Part one Sequence variation of p24 coding region in gag gene of human immunodeficiency virus type 1,Acquisition of HIV-1 p24 protein region encoding gene and gp41 protein region encoding geneObjective To acquire HIV-1 p24 protein region encoding gene and gp41 protein region encoding gene using by RT-PCR.
     第一部分 HIV-1p24蛋白编码区基因变异性的研究以及p24蛋白和gp41蛋白编码区基因片段的获得目的:用RT-PCR方法获得HIV-1p24蛋白编码区基因片段以及gp41截短体蛋白编码区基因片段。
短句来源
     To comprehend the variation of p24 coding region in p24 protein of HIV-1 in China.
     了解我国HIV感染者p24蛋白编码区的基因变异情况。
短句来源
     Aim Expressing the capsid protein (p24) of human immunodeficiency virus type 1 (HIV-1 ) for preparing monoclonal antibody against HIV. Methods The p24 gene fragment encoding the p24 protein was recombined and inserted into expression vector PET─ 17b under the control of the bacteriophage T7 promoter.
     目的表达人Ⅰ型免疫缺陷病毒(HIV—1)衣壳蛋白p24,为制备抗p24单克隆抗体及其诊断杭原奠定基础。 方法将编码HIV—1p24蛋白的P24(gag)基因片段,克隆到原核表达载体PET—17b的T7噬菌体启动子下游,构建重组表达质粒;
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  p 24 antigen
     Conclusion Sensitive and specific double antibodies sandwich to detect p24 antigen of HIV-1 were established and 50 pg/mL p24 antigen can be detected by the indirect method.
     结论:建立了敏感、特异的检测p24蛋白的双抗体夹心法,间接放大方法可检出50pg/mL的HIV-p24蛋白,检测敏感性与国际同类产品相似。
短句来源
     Detection of p24 Antigen of HIV-1 for Early Diagnosis
     HIV感染早期病毒p24蛋白的检测
短句来源
     The spread of HIV 1 in the cultures was determined by quantitating the levels of HIV 1 p24 antigen released into the culture medium.
     以HIV 1NL4 3病毒株感染表达IN sFv的SupT1及PBMC ,用ELISA方法测定病毒感染细胞后不同时间培养上清中HIV 1P24蛋白的含量 ,以监测病毒复制的水平 ;
短句来源
     Objective To establish a sensitive and specific method for detection of HIV-1 p24 antigen as early diagnosis of HIV infection.
     目的:建立敏感、特异的检测血清中HIV-p24蛋白的方法,作为HIV感染早期即窗口期的监测手段。
短句来源
     The antibodies against recombinant p24 protein from HIV infected plasma were purified and confirmed with HIV Western blot kit that the purified antibodies can only react with HIV 1 p24 antigen.
     以此蛋白交联Sepharose 4B ,亲和层析纯化HIV感染者血清中的抗体 ,用所得抗体与HIV确认试剂反应 ,发现该纯化抗体仅与确认试剂中的 p2 4蛋白反应。
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  p 24
     Expression,purification and identification of Gag P55 and P24 recombinant protein of HIV-1 strain CN54
     HIV-1 CN54 Gag P55和P24蛋白的高效表达、纯化和鉴定
短句来源
     SDS PAGE proved that 46% of recombinant p24 protein was expressed in BL21(DE3)plysS.
     SDS PAGE分析表明 ,受体菌BL2 1(DE3)plysS表达量最高 ,表达的重组p2 4蛋白占菌体总蛋白的 46 %。
短句来源
     High Level Soluble Expressiion, One Step Purification and Characterization of HIV-1 p24 Protein
     HIV-1p24蛋白在大肠肝菌中的高效可溶性表达、一步纯化及抗原性分析
短句来源
     Part one Sequence variation of p24 coding region in gag gene of human immunodeficiency virus type 1,Acquisition of HIV-1 p24 protein region encoding gene and gp41 protein region encoding geneObjective To acquire HIV-1 p24 protein region encoding gene and gp41 protein region encoding gene using by RT-PCR.
     第一部分 HIV-1p24蛋白编码区基因变异性的研究以及p24蛋白和gp41蛋白编码区基因片段的获得目的:用RT-PCR方法获得HIV-1p24蛋白编码区基因片段以及gp41截短体蛋白编码区基因片段。
短句来源
     Results rM-GP for stable and high expression of HIV-1 Gag-Pol was screened. Western blot showed specific antibody against HIV-1 p24 in the sera of mice immunized with rM-GP.
     结果已筛选到稳定高效表达HIV-1Gag-Pol蛋白的重组痘病毒载体rM-GP,经Westernblot,在免疫小鼠血清中检测到抗HIV-1p24蛋白的特异性抗体。
短句来源
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  “p24蛋白”译为未确定词的双语例句
     The biological and immunological characteristics of HIV 1 core protein p17 24 expressed by recombinant vaccinia viruses were studied.
     研究了重组痘苗病毒表达的HIV1核心蛋白(Gag)p17p24蛋白的一些生物学及免疫学特点。
短句来源
     3*3 factorial design was used to explore the yield optimization of this system when BHK21 was utilized as the production cell line for that it was the best cell line for the LV production among the three mentioned cell lines containing BHK_(21), Vero, and HepG_2. the SPSS analysis results illustrated that the plasmids dosage and the MOI have significant effects on the system output respectively, and these two factors have significant interaction.
     当三质粒与辅助痘苗病毒共转染生产细胞BHK_(21),48h后,共聚焦显微镜下观察到生产细胞表达p24蛋白,并且其主要分布于胞质中,而在细胞核中基本不表达,这提示质粒pGAGPOL构建成功; 普通倒置荧光显微镜下观察到生产细胞表达GFP,提示质粒pVECRNA构建成功,以上结果也提示生产系统正常工作。
短句来源
     coli. Western blotting and immunodot blotting indicated that the recombinant protein could be recognized by anti-p24 monoclonal antibody and HIV-1 positive sera respectively.
     重组p24蛋白均与抗p24单克隆抗体及HIV—1阳性血清发生特异性反应。
短句来源
     Conclusions The recombinant protein is useful for preparing specific antibody and diagnostic usage.
     结论重组P24蛋白具有较好的免疫活性,是制备抗P24单克隆抗体及诊断试剂的理想抗原。
短句来源
     Westein blotting and,immunodot blotting indicated that the recombinain protein could be relognized by antip24 monoclonal antibody and HIV-1 positive sera respectively. It is significance for preparing specific antibody,and diagnostic usage.
     重组P24蛋白均抗p24单克隆抗体及HIV—1阳性血清发生特异性反应,具有较好的抗原性,对研究开发AIDS诊断试剂和基因工程疫苗具有重要意义。
短句来源
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  p 24 protein
Our positive result shows that the expressed p24 protein could be used as standard antigen for HIV-1 diagnosis by ELISA and other reliable diagnostic methods of HIV-1 infection.
      
In planta expression of HIV-1 p24 protein using and RNA plant virus-based expression vector
      
Production of HIV-1 p24 protein in transgenic tobacco plants
      
Western blot analysis of protein extracts from transgenic plants identified plant-expressed p24 protein that cross-reacted with a p24-specific monoclonal antibody, thus confirming the maintenance of antigenicity.
      
Quantification of the p24 protein using enzyme-linked immunosorbent assay (ELISA) estimated yields of approx 3.5 mg per g of soluble leaf protein.
      
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  p 24 antigen
Brain tissue from 10 autopsy and 6 biopsy specimens was studied using: in situ hybridization (ISH) for JC virus (JCV), immunohistochemistry for human immunodeficiency virus (HIV) p24 antigen, and electron microscopy.
      
During the entire treatment period a decline in p24 antigen was observed in all patients.
      
The presence of p24 antigen could be demonstrated in only 16% of the sera.
      
Immune complex p24 antigen: A new prognostic marker in human immunodeficiency virus infection
      
p24 antigen (p24 Ag) is usually not found in patients in the asymptomatic phase of HIV infection, mainly because it is bound to anti-p24 antibodies (p24 Ab), with which it forms immune complexes.
      
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  p 24
Exogenous PARP1 and p24 decreased the efficiency of gap filling DNA synthesis for both duplexes, but did not influence the ligation stage in the repair of DNA duplex by the short-patch subpathway.
      
Addition of exogenous PARP1 and p24 also reduced the efficiency of UV light crosslinking of extract BER proteins to the photoreactive BER intermediates carrying a nick.
      
Thus, PARP1 and p24 interact with DNA intermediates of BER and compete with nuclear extract proteins for binding to DNA.
      
The interaction of PARP1 and p24 with DNA intermediates of the long-patch subpathway of BER resulted in inhibition of subsequent stages of the repair mediated by this mechanism.
      
However, on recovery of the intact structure of DNA duplex by the short-patch subpathway, PARP1 and p24 suppressed the repair of the one nucleotide gap less efficiently and failed to influence the final stage of the repair, ligation.
      
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A detached garlic scape in long storage will eventually give rise to a whorl of freshy aerial cloves at its apex(Text fig.2).This can only be brought about at the expense of the stalk proper,where withering starts from the lower end and extends gradually up- ward until the whole stalk is completely exhausted.The material transfer involved must be mainly concerned with the redistribution and reultilization of cellular contents from the senescing stalk to the growing cloves.The present systematic investigation...

A detached garlic scape in long storage will eventually give rise to a whorl of freshy aerial cloves at its apex(Text fig.2).This can only be brought about at the expense of the stalk proper,where withering starts from the lower end and extends gradually up- ward until the whole stalk is completely exhausted.The material transfer involved must be mainly concerned with the redistribution and reultilization of cellular contents from the senescing stalk to the growing cloves.The present systematic investigation on the whole process is primarily based upon serial microscopic and electron-microscopic examination on conducting channels and withering parenchyma. Our previous investigations on garlic have shown that the exhaustive withdrawl of cellular contents from the senescing tissue is finally accomlished by intercellular move- ment of the partially disassembled protoplasm itself.The present result are essentially in agreement with such a general scheme.Light-and electron-micrographs that show nuclear material and other macro-molecular substances tranversing through the plasmo- desmata are rather common.The high resolving electron-micrographs have enabled us to detect the finer details in intercellular transport as given below: 1.Filamentous and fluffy material,somewhat similar in structure to P-protein in sieve tube,can be found in abundance in senescing parenchyma cells in which the demar- kations between protoplasmic components gradually become indistinct.The filamentous material is in transit through plasmodesmata between parenehyma cells and also between parenchyma and sieve tube(Plate Ⅱ,16,18). 2.Withdrawl of cellular contents from the deteriorating parenehyma may assume the form of vesicular transport through plasmodesmata(Plate Ⅰ 9,10,11).Some of the vesicles are simply filled with vacuolar sap; some fully packed with prefabricated ma- terial of macro-molecular structure;and some actually loaded with disassembled pro- toplasmic fragments. 3.Fully packed vesicles as well as disassembled protoplasmic components(includ- ing disintegrated nucleus,degenerated mitochondrion,etc.) may extrude into the intercel- lular spaces and may invade the vessel cavity (Plate Ⅱ,12,13,20; Plate Ⅲ,21,22,23, 24).The fine strueture of the moving protoplasm in the vessel is quite distinct from that of the residual deposits which may cause plugging in the same cavity(Plate Ⅲ, 25,26).

以贮存蒜苔为材料,研究了在茎苔衰退与顶端珠蒜成长过程中物质的再分配,及其在显微、亚显微结构上的反映。观察到类 P-蛋白丝状物在衰退细胞中的形成及其经胞间连丝在薄壁细胞间、薄壁细胞与韧皮部之间进行运转的多种迹象,提出了大分子物质以集装囊泡的形式进行运输的新论点。进一步发现大分子物质在质外体(导管与细胞间隙)中存在与迁移状态;初步论证了它们与导管堵塞物在质上的差异;指出在特定生理状态下,质外体提供的“自由空间”作为大分子物质主动迁移途径的可能性。

The minor leaf vein of Populus deltoides Bartr. cv. 'Lux' (ex. I-69/55), and P. ×euramericana (Dode) Guinier cv. 'San Martino' (ex. I-72/58) has no A-type and B-type transfer cells, which exist in the herb dicotyledon. But some structures like compound plasmodesma and paramural body that vary morphologically with the development of the leaf can be produced by the companion cells and the phloem parechyma cells of the minor leaf vein. It's shown that their occurrence has something to do with the frequent local...

The minor leaf vein of Populus deltoides Bartr. cv. 'Lux' (ex. I-69/55), and P. ×euramericana (Dode) Guinier cv. 'San Martino' (ex. I-72/58) has no A-type and B-type transfer cells, which exist in the herb dicotyledon. But some structures like compound plasmodesma and paramural body that vary morphologically with the development of the leaf can be produced by the companion cells and the phloem parechyma cells of the minor leaf vein. It's shown that their occurrence has something to do with the frequent local transport. The sieve tube members in the minor leaf vein are differentiated through the formation of the P-protein body, the function of the lysosome in the vacuome and the secretion of the dictyosome. In the two clones of the Aegeiros poplar, i.e., I-69 and I-72 there exist distinctions. It seems that the distinctions are related to the increment. Therefore, they require further research.

Populus delioides Bartr.ev.‘Lux’(ex.I-69/55),和P. ×euramericana(Dode) Guinier ev.‘San Martino’.(ex.I-72/58)无性系后代实生苗叶小脉不具有草本双子叶植物中的A型和B型传递细胞(transfer cell),但小脉伴胞和韧皮薄壁细胞的某些结构-复合胞间连丝和壁旁体(paramural body)的形态是随叶片发育而变化,表明它们的发生与局部频繁的运输有联系。小脉筛管分子的分化是通过 P-蛋白体(P-protein body)的形成和液泡系的溶酶体作用,并伴之以高尔基体的分泌作用。I-69和I-72无性系后代实生苗叶小脉的超微结构有区别,似与生长量有一定的相关性,值得深入研究。

A comparative study on the ultrastructural localization of ATPase activity in the cells of the fifth internode of different species was carried out with cytochemical technique.In the phloem cells of sugarcane stem,ATPase activity was localized on the plasma membrane of sieve elem- ents and companion cells,the nuclei,vescles,full development vacuole of companion cell and P-protein.The ATPase activity showed obvious diffe- rence in the stem phloem cells of varous species.The ATPase activity in the phloem ceils...

A comparative study on the ultrastructural localization of ATPase activity in the cells of the fifth internode of different species was carried out with cytochemical technique.In the phloem cells of sugarcane stem,ATPase activity was localized on the plasma membrane of sieve elem- ents and companion cells,the nuclei,vescles,full development vacuole of companion cell and P-protein.The ATPase activity showed obvious diffe- rence in the stem phloem cells of varous species.The ATPase activity in the phloem ceils of wild species and original cultigens was higher than that of commercial varietes.The results suggested that the phloem ATPase acti- vity in the sugarcane stem be associaed with the transport and accumulation of sugar and the resistibility,and there be P-protein ATPase taking part in the phloem transport of sugarcane stem.

采用酶细胞化学技术对7个甘蔗种和品种进行研究。甘蔗茎韧皮部细胞 ATP 酶活性定位于筛管、伴胞质膜、伴胞核、小囊泡、充分发育的液泡膜和 P—蛋白上。野生种和栽培种茎韧皮部细胞 ATP 酶活性较高,而生产品种则较低。认为甘蔗茎韧皮部 ATP 酶活性与糖分的运输和抗性等有关。茎韧皮运输中,可能有 P—蛋白和 ATP 酶主动参与。

 
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