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single nucleotide primer extension
相关语句
  单碱基引物延伸反应
     This study explored a Y-STR multiplex system with nine loci and a multiplex Y-SNP typing assay with single nucleotide primer extension (SNuPE).
     建立多重Y-SNP单碱基引物延伸反应,掌握多重单碱基引物延伸反应的一般规律,为高通量分析Y-SNP提供基础。
短句来源
  “single nucleotide primer extension”译为未确定词的双语例句
     To quantify the products of single nucleotide primer extension (SNuPE), the single strain oligonucleotides were separated by High Performance Liquid Chromatography (HPLC).
     建立用普通高效液相色谱仪检测单链寡聚核苷酸的方法,为检测单碱基引物延伸反应的产物提供定量分析基础。
短句来源
     The aim of the present study was to establish a rapid and robust assay to simultaneously genotype SNP by the single nucleotide primer extension (minisequencing) with PCR multiplexes.
     方法 采用商品试剂SNaPshot对SNP位点进行复合PCR和复合微测序检测分析。
短句来源
  相似匹配句对
     Single Nucleotide Polymorphisms
     单核苷酸多态性
短句来源
     Arrhythmia and single nucleotide polymorphism
     心律失常与单核苷酸多态性
短句来源
     from single to unity;
     从单一到统一;
     Single thinking
     思维单一是缺陷
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     Primer,200nM;
     Primer,200 nM;
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  single nucleotide primer extension
These base substitutions were confirmed in mRNA extracted from homozygous mutants using a Single Nucleotide Primer Extension assay (SNuPE) and are sufficient to produce thermolabile enzyme.
      
Towards this, we have applied a highthroughput Single Nucleotide Primer Extension assay to assess 23 polymorphicbase variations at five microsatellite loci in 22 inbred maize lines, as wellasin a mapping population of two of the inbred lines.
      
An array consisting of four genes in two Japanese participants, A121 and A447, was detected either by pulsed field gel electrophoresis and subsequent Southern hybridization or by single nucleotide primer extension reaction.
      
The 4 alleles could be identified unambiguously by codominantly genotyping 2 of the cSNPs using a duplex single nucleotide primer extension (SNuPE) assay.
      
Single nucleotide primer extension was applied to discriminate between Cpn10-rs1 and Cpn10 expression.
      
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Objective The aim of the present study was to establish a rapid and robust assay used to simultaneously genotype SNPs by the single nucleotide primer extension (minisequencing) with the SNaPshot Kit and obtain the population genetic data in Chinese population in Sichuan. The analysis of single nucleotide polymorphisms (SNPs) is a promising application in forensic casework. Methods 12 Y-SNPs, which were SRY2627, SRY1532, M13, M20, SRY8299, Tat, M69, M9, 92R7, M17, M19 and M112, were multiple...

Objective The aim of the present study was to establish a rapid and robust assay used to simultaneously genotype SNPs by the single nucleotide primer extension (minisequencing) with the SNaPshot Kit and obtain the population genetic data in Chinese population in Sichuan. The analysis of single nucleotide polymorphisms (SNPs) is a promising application in forensic casework. Methods 12 Y-SNPs, which were SRY2627, SRY1532, M13, M20, SRY8299, Tat, M69, M9, 92R7, M17, M19 and M112, were multiple amplificated and the PCR products were pooled , Purified, and then used as templates for the minisequencing reaction with the commol/Lercially available SNaPshot Kit. Then the products of minisequencing reaction were detected by capillary elcetrophoresis. Results 78 genomic DNA individual samples from Sichuan, China and 5 semen stain samples from sexal criminal scene were analyzed and two haplotypes could be identified. Conclusion A rapid method has been established to analyze the 12 Y-SNPs by multiplex PCR and minisequencing. It can be applied in forensic casework successfully.

目的应用SNaPshotKit对Y染色体上12个SNP位点进行快速而准确的检测,对四川地区78个汉族男性无关个体进行群体遗传学研究,并对陈旧骨骼和性犯罪案件的相关物证进行检验。方法对SRY2627、SRY1532、M13、M20、SRY8299、Tat、M69及M9、92R7、M17、M19、M112两组共12个Y-SNP位点进行复合扩增,PCR产物经纯化处理后,采用SNaPshotKit试剂结合毛细管电泳技术对单核苷酸多态性进行检测。结果建立了12个Y-SNP位点的微测序快速检测系统,在四川地区人群中发现M9、SRY8299二个位点存在变异。结论复合扩增结合微测序技术能够同时对多个Y-SNP的多态性进行快速而准确的检测,建立的检测系统在法医学个体识别中具有应用价值。

 
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