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mature rat
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  成年大鼠
     2, Healthy and mature rat with weight between 200g and 240g was selected .
     2.取健康成年大鼠,体重200g-240g,麻醉后固定于立体定向仪。
短句来源
     METHODS: With Langendorff perfusion, we observed DMA and AM induced changes of the indexes reflecting cardiac function in immature rat hearts and compared with mature rat hearts.
     探讨DMA对幼年大鼠心脏功能及乳头肌影响的可能作用机制。 方法:采用Langendorff心脏灌流方法观察和比较DMA和AM分别对幼年及成年大鼠离体心脏功能的影响;
短句来源
     Methods Used immunohistochemistry methods and electron microscope to observe the shape and the distribution of IEL and LPL which come from the mature rat.Results CD4 + IEL and LPL increased along the duodenum,jejunum,ileum and sigmoid flexure;
     方法 采用免疫组织化学方法及光镜观察成年大鼠肠道各段IEL与LPL的形态和分布特征。 结果 CD4 + T细胞在IEL与LPL中均沿十二指肠、空肠、回肠、结肠递增 ;
短句来源
     Results: ①In the mature rat,c-fos expression was found higher in interstitial gland and stroma of proestrous ovaries and lower in estrous ovaries ,and was found higher in luteal cells and stroma of pregrnant ovaries and lower in diestrous ovaries.
     结果 :在成年大鼠动情前期、动情期卵巢的间质腺和基质中及动情间期和妊娠期卵巢的黄体细胞和基质中有c fos表达 ,c fos蛋白阳性信号的表达范围和表达强度在动情前期卵巢较大 ,动情间期和动情期卵巢较低 ,妊娠期卵巢最大 ,这种变化与血清E2 和P含量的变化呈明显的正相关关系。
短句来源
     Cyclooxygenase-2 expression and its localization in the mature rat testis
     环氧化酶-2在正常成年大鼠睾丸中表达的研究
短句来源
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  成熟大鼠
     Objective: To investigate the effects of thyroid hormone on regeneration of mature rat Leydig cells by observing the regeneration of Leydig cells after ethane dimethane sulphonate (EDS) treatment with NBT method, HE staining and magnetic enzyme immunoassay method under hyperthyroidism and hypothyroidism condition.
     目的:应用硝基蓝四唑盐(NBT)法、HE染色和磁分离均相酶联免疫法等方法观察睾丸间质细胞(Leydig cells, LCs)被二甲磺基乙烷(ethane dimethane sulphonate,EDS)破坏后在甲状腺机能亢进和甲状腺机能减退情况下的再生情况,探讨甲状腺激素对成熟大鼠LCs再生的影响。
短句来源
     Homology searches revealed that DD1-35, DD2-81 , DD5-40 are new cDNA sequence. Northern blot analvsis of DD5-40 confirmed that it was ex-pressed specifically in sexual mature rat testis .
     以DD5-40为探针,进行Northern杂交,发现此片段只与性成熟大鼠睾丸RNA有杂交信号。
短句来源
     Objective To explore the expression and proliferation of neural stem cell induced f ollowing brain injury of mature rat.Methods The cortex and caudate nucleus of mature SD rats were cut transversely in s tereotaxic apparatus and animals we re killed in 1week,2weeks,3weeks and 4weeks following injury.
     目的探讨成熟大鼠在脑组织受损后神经干细胞的表达与增殖情况。 方法通过立体定向仪横断成熟大鼠皮质和内囊处的尾状核,于术后1、2、3、4、5周分别杀死动物。
短句来源
     Expression and proliferation of neural stem cell induced following brai ninjury of mature rat
     成熟大鼠脑损伤后神经干细胞的表达与增殖
短句来源
     Conclusion: The neural stem cell usually stay in the SVZ in the brain of mature rat. The damage of brain can induce the expression and proliferation of neural stem cells around SVZ and wound, and neural stem cells play a chief role in repairing.
     结论:第网军医大学硕士学位论义成熟大鼠神经干细胞主要分布在侧脑室室管膜下区,脑损伤后可诱导室管膜下区的神经干细胞增殖,进而表达为伤D周围的神经干细胞梯度递减反应模式,提示神经于细胞对于脑组织损伤后的修复起着重要作用。
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  “mature rat”译为未确定词的双语例句
     Differences of distribution between CD4~+ IEL and LPL in intestinal trace of mature rat
     CD4~+T细胞在大鼠肠道各段IEL与LPL中的分布差异
短句来源
     Differences of Distribution of CD8~+ T Lymphocyte Subgroup Between IEL and LPL in Intestines of Mature Rat
     CD8~+T细胞亚群在大鼠肠道各部IEL与LPL中的分布差异
短句来源
     To obtain recombinant glial cell line derived neurotrophic factor receptor alpha1 (GFRα1) and study its biological activity, the cDNA encoding the mature rat GFRα1 was isolated using RT PCR with total RNA extracted from newborn SD rat hippocampus tissue.
     为了获得重组胶质细胞源性神经营养因子受体α1(glialcellline derivedneurotrophicfactorreceptoralpha1,GFRα1)并研究其生物学活性 ,从新生 4天的SD大鼠海马组织中提取总RNA ,通过RT PCR方法 ,扩增出GFRα1cDNA .
短句来源
     The expression and phosphorylation of mitogen-activated protein kinases (MAPK) and the expression of p90 rsk , one of the substrate of MAPK, in immature and mature rat follicle growth have been investigated by immunohistochemical staining with antibodies against ERK2, active MAPK and p90 rsk.
     利用免疫组织化学方法研究丝裂原激活蛋白激酶 (mitogen activatedproteinkinases,MAPK)及其底物之一p90 rsk在大鼠卵泡发育过程中的表达与活性。
短句来源
     Using RT PCR method, the cDNA encoding the mature rat GDNF was isolated with total RNA extracted from newborn SD rat hippocampus tissue.
     从新生 4天的SD大鼠海马组织中提取总RNA ,通过RT PCR方法 ,扩增出GDNFcDNA。
短句来源
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  mature rat
The effect of thyroid status on noradrenergic synaptic function in the mature rat brain was examined by measuring presynapticα2- and post-synapticβ-adrenoceptors.
      
The data show that the dorsomedial hypothalamic nucleus is involved in hypothalamic mechanisms controlling food intake, water intake and spontaneous activity not only in the weanling rat as previously demonstrated, but in the mature rat as well.
      
Effect of prenatal exposure to ethanol on the ultrastructure of layer V of mature rat somatosensory cortex
      
The surfaces of cells of the ependyma and choroid plexus in the lateral ventricle of the mature rat brain have been examined with a scanning electron microscope on tissue dried by the critical point method.
      
Accordingly, we examined substance P release from cultures of mature rat sensory neurons, which do not require NGF for survival.
      
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The young and mature rats wereused to study the effects of gossypol ontesticular seminiferous epithelium.Theyoung rats after 30 days of age wereadministrated with gossypol 20mg/kgbody weight 5 times weekly for 1 and2 months.The mature rats treatedwith gossypol 5mg daily 5 times aweek for long-terms.The rats weresacrificed after 1,2,3,4 and 6 monthsadministration and withdrawal for 1、2and 3 months.The testis were examinedhistologically.The results indicated that the tes-tis of young rats...

The young and mature rats wereused to study the effects of gossypol ontesticular seminiferous epithelium.Theyoung rats after 30 days of age wereadministrated with gossypol 20mg/kgbody weight 5 times weekly for 1 and2 months.The mature rats treatedwith gossypol 5mg daily 5 times aweek for long-terms.The rats weresacrificed after 1,2,3,4 and 6 monthsadministration and withdrawal for 1、2and 3 months.The testis were examinedhistologically.The results indicated that the tes-tis of young rats treated with gossypolfor 1 month developed normally exceptthe sperms in the caudal epididymisand vas deferens were dead.This con-firmed our previous finding that themost sensitive elements of the semini-ferous epithelium with gossypol werespermatids in late spermiogenesis.When the rats were treated more thantwo months,testicular atrophy appea-red in part of the rats.Since the num-ber and degree of degeneration of testiswere closely correlated to the length ofduration of treatment,it seemed thattestis atrophy might be caused by gos-sypol directly.However,the occurren-ce of damaged spermatids at steps 18and 19 were really earlier than testi-cular atrophy.The first sign of thelater was the accumulation of deadsperms in the seminiferous tubule adja-cent to rete testis and degenerationalways began from there.It may bepostulated that testicular atrophy iscaused by the dead sperms accumu-lated in the tubules.

选用出生1月龄大白鼠按20毫克/公斤体重/日灌服棉酚,每周5次,连续1个月和2个月取材;性成熟大白鼠每天服棉酚5毫克/只,每周6次,给药3、4、6个月和停药1、2、3个月分别取材,睾丸称重后固定、制片作比较观察。实验结果表明,棉酚对幼龄大白鼠睾丸的发育没有影响,但使发育到变态后期的精子细胞受损伤,附睾中出现断头坏死的精子。长期服抗生育有效剂量棉酚的大白鼠中,有相当一部分出现不同程度的睾丸退化。退化通常在睾网附近最先出现,并和死精子在曲精细管中积聚相关连。第Ⅶ—Ⅷ期生精上皮退化的过程是自上皮游离缘18—19期精子细胞开始渐进地向基膜方向推进,直至上皮完全剥落或仅留以个别精原细胞和较多的媬育细胞。停服棉酚后恢复1—3个月的结果指出,已开始退化的睾丸在停药后有继续退化的趋势。严重退化的睾丸经停药3个月,虽然曲精细管排列较整齐,部分留有精原细胞的曲精细管中精原细胞显示增加数目并有B 型精原细胞,但尚未见进一步发展。对于睾丸退化可能与死精子在曲精细管内积聚有关,而不是由于棉酚对整个生精上皮的损伤问题进行了讨论。

It has been reported that about 6% volunteers administered with gossypol complained of libido decreasing. However, serum testosterone and LH level measured in part of the volunteers were not in accordance with their complaint. Even if the volunteers felt libido decreasing, the serum concentrations of testosterone were still in normal range. On the other hand, only a few informations obtained from animal experiments suggested that gossypol did not affect the serum concentrations of the testosterone and LH in...

It has been reported that about 6% volunteers administered with gossypol complained of libido decreasing. However, serum testosterone and LH level measured in part of the volunteers were not in accordance with their complaint. Even if the volunteers felt libido decreasing, the serum concentrations of testosterone were still in normal range. On the other hand, only a few informations obtained from animal experiments suggested that gossypol did not affect the serum concentrations of the testosterone and LH in male rats. Clinically this phenomenon has not been interpreted. In addition, testis atrophy were revealed in rats and in men administrated with gossypol for long-terms. It may be considered whether testicular degeneration is caused by decreasing of testosterone or not.With radioimmunoassay, the serum concentrations of testosterone and LH were determined in immature and mature rats (Wistar strain and with known birthday) after treatment of gossypol. The experiments were divided into several groups as follows. The first group, immature rats at 30 days age were treated with gossypol at the dosage of 20 mg/kg body weight, 5 times a week for two months; the second, immature rats treated on the same condition as in group one and the drug was withdrawn for one month; the third, mature rats administrated with gossypol at 5 mg/day 5 times weekly for one month and six months. Untreated normal immature and mature rats in the same ages were used as control of each group. The animals in each group were sacrified on time, and blood samples were collected separately. For comparison with themselves mature rats were treated with gossypol monthly and withdrawal for a month, blood was collected by cardiac puncture before and after treatment.Serum testosterone was measured by method of Corker et al. (1978) without chromatography. Serum LH was determined by ovine-ovine method of Niswender et al. (1968), using NIH-LH-S 18 as reference standard. The purified ovine LH 2-2-1 was labelled with ~(125)I by the method of Greenwood et al. (1963), and the purification of ~(125)I-LH2-2-l was performed according to Wang et al. (1979).The results indicate that serum concentrations of testosterone decreased significantly(P<0.025) in immature and mature rats by treatment of gossypol for different time intervals, and the evocation of testosterone level was not found after cessation for a month. However, there was no change in serum level of LH in all groups. The decrease of testosterone is not connected with the body weights and testisa trophy. It was concluolod that the decrease of serum testosterone might be due to a direct effect exerted by gossypol on Leydig cells, and the accumulation of gossypol in the testis might result in the concentration of teststerone maintaining at a lower status.

用放射免疫测定法分析了服棉酚雄性大白鼠血清睾丸酮和LH的含量。数据表明,无论是幼龄大白鼠服棉酚2个月和服棉酚2个月停服1个月的,或是性成熟大白鼠服棉酚1个月和停服棉酚1个月的以及服棉酚6个月的血清睾丸酮食量都明显低于同月龄对照大白鼠的或服棉酚前的水平。但血清LH含量没有明显改变。血清睾丸酮含量降低和体重增长以及睾丸生精上皮退化与否都没有明显相关。根据现有的资料可以认为,服棉酚大白鼠血清睾丸酮含量减低,是由于棉酚直接作用于睾丸间质细胞的结果。停服棉酚后睾丸酮含量,除个别动物外均未见明显回升,可能由于棉酚在体内有一定的积累所致。

The scrotum of mature rats was immersed in a hot water bath (43±0.2℃) for 25 min once each month. One to seven days after the first treatment, the lysosomes, lipid droplets and mitochondria significantly increased in number in the sertoli cells. It also showed that heat treatment accelerated the mitochondria's degenerative changes and caused the mitochondria temporarily to multiply. The exfoliation of the sertoli cells into tubular lumen were often observed during seven to thirty days after the first heating,...

The scrotum of mature rats was immersed in a hot water bath (43±0.2℃) for 25 min once each month. One to seven days after the first treatment, the lysosomes, lipid droplets and mitochondria significantly increased in number in the sertoli cells. It also showed that heat treatment accelerated the mitochondria's degenerative changes and caused the mitochondria temporarily to multiply. The exfoliation of the sertoli cells into tubular lumen were often observed during seven to thirty days after the first heating, the exfoliated sertoli cells became more numerous after the second or the third heating.

成年大鼠睾丸接受43±0.2℃温水浴25min,每月处理一次。首次加温后1~7天,支持细胞内的溶酶体、脂滴和线粒体数量明显增多。结果指出,热处理不但引起线粒体暂时性增生,同时也加速其变性和解体过程。加温后7~30天,常见支持细胞脱落入管腔,第二次和第三次处理后更为常见。电镜显示,加热对间质细胞的超微结构无损害,但首次加温后2~7天,间质组织内的胶原纤维出现增生。每月加温一次,二次和三次处理后,所有睾丸都呈现纤维化。每65天加温一次,二次处理后,仅见一例睾丸出现轻度纤维化。上述结果表明,睾丸纤维化程度与两次热处理之间的恢复时间长短有关。

 
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