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selective markers
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  选择标记
     A disarming K 599 contains selective markers of resistance to ampicillin/carbencillin and the growth inhibitation by 10% sucrose.
     解毒后的K599获得了氨苄 /羧苄青霉素抗性和 10 %蔗糖抑制生长的选择标记
短句来源
     Using BPV Transforming Foci as Selective Markers to Express HBsAg
     用BPV转化灶做选择标记表达HBsAg的研究
短句来源
     This article studied the inheritance mechanisms of this cultivar from classical and molecular genetics, which will offer the selective markers for marker assisted selection.
     本论文从经典遗传学和分子遗传学方面对该品种的抗条锈性遗传表达规律进行了较系统的研究,以揭示外源抗病基因的遗传学特点,并为分子标记辅助育种提供选择标记
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     Some transformation methods,selective markers,and the application of transformation system in the field of research on filamentous fungi are reviewed in this paper.
     对丝状真菌遗传转化系统的最新研究进展进行综述,主要包括丝状真菌的转化方法、选择标记、转化系统的应用等。
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     Breeding Transgenic Plants with Safe or No Selective Markers
     培育具有安全选择标记或无选择标记的转基因植物
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  “selective markers”译为未确定词的双语例句
     Using 50% PEG 1000 as a inducer and the antibiotic resistance as selective markers, the fusion frequencies were 10 -2 for 9011×WH1 and 10 -3 for both FR008×9011 and FR008×WH1.
     以50%PEG1000诱导融合各组合的融合频率是,90-11×WH-1为10-2,FR-008×-90-11以及FR-008×WH-1均为10-3。
短句来源
     Breeding of Transgenic Wheat with Leaf Senescence-inhibition Gene P_(SAG12)IPT and without Selective Markers
     无筛选标记的转 P_(SAG12)-IPT基因小麦的选育
     coli,which has the same selective markers as pBR328.
     coli之间的穿梭载体即pZB31,其具有与pBR328完全相同的抗性标记。
短句来源
     Using calcium phosphate precipitation technique to transform C127 Cells and using BPV transforming foci as selective markers, we obtained transformed cell clones.
     用磷酸钙沉淀技术把此质粒导入鼠C_(127)细胞内,用BPV转化灶做标记,获得了转化细胞克隆。
短句来源
     coli is constructed by inserting the 2.7-kilobasepair (kb) cryptic plasmid pZM2 of Zymomonas mobilis ATCC 10988 into the Escherichia coli vector pBR328 in the site of SphI digests. The new vector, 7.656 kb plasmid pZB1, has two selective markers, Apr and Cmr.
     将由Z. mobilis ATCC10988中分离出的2.7kb质粒pZM2,插入大肠杆菌载体质粒pBR328的SphI酶切位点,构建成大小约为7.6kb的重组穿梭质粒(pZB1)。
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  相似匹配句对
     coli,which has the same selective markers as pBR328.
     coli之间的穿梭载体即pZB31,其具有与pBR328完全相同的抗性标记。
短句来源
     were its chromosome markers.
     等为其标志染色体,未显示有完整Y染色体存在。
短句来源
     Application of Morphological and Microsatellite Markers in Selective Breeding of Fenneropenaeus Chinensis
     形态标记与微卫星标记在中国对虾遗传选育中的应用研究
短句来源
     SELECTIVE FLOCCULATION
     选择性絮凝作用
短句来源
     Selective Chlorination of o - Xylene
     邻二甲苯选择性氯化的研究
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  selective markers
DNA mediated gene transfer using Simian virus 40 or Polyoma virus morphological transformation as selective markers
      
All these phenotypes correlated with expression of ORF VI in three lines of transgenic plants which were produced independently, with different Ti-plasmid derived vectors and with different selective markers.
      
Although a number of selective markers are known for variouslineage-limited hematopoietic cells and their progeny,our understanding of the biology of the precursor cellsfor mammary epithelium is just beginning.
      
Such a construction when combined with appropriately placed selective markers should prevent breakdown of the complex, and should resemble an inversion in eliminating crossover products.
      
Calcium-binding proteins: selective markers of nerve cells
      
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Direct transformation of Streptomyces griseus No.45-3 (Lin5, Penr, Amy- , Str- ) was performed successfully with the plasmid DNA isolated from S. griseus No. 45 (Lin, Pen5, Amy+. Str+), a streptomycin producing strain. Lincomycin resistance was used as a selective marker. Optimal conditions for transformation have been determined: recipient cell in early logarithmic phase, DNA treatment 60 minutes, and CaCl2 concentration in the transformation liquid 10-6 M. Among the lincomycin resistant transfor-mants...

Direct transformation of Streptomyces griseus No.45-3 (Lin5, Penr, Amy- , Str- ) was performed successfully with the plasmid DNA isolated from S. griseus No. 45 (Lin, Pen5, Amy+. Str+), a streptomycin producing strain. Lincomycin resistance was used as a selective marker. Optimal conditions for transformation have been determined: recipient cell in early logarithmic phase, DNA treatment 60 minutes, and CaCl2 concentration in the transformation liquid 10-6 M. Among the lincomycin resistant transfor-mants selected about 65.9 % resumed the ability of streptomycin biosynthesis. These results further verified that plasmid is involved in streptomycin biosynthesis in S. griseus.

我们以链霉素产生菌——灰色链霉菌No.45为给体(Lin~r、Pen~s、Amy~+、Str~+),以高温消除质粒的No.45-3(Lin~s、Pen~r、Amy~-、Str~-)为受体,以Lin~r为筛选标记,进行转化试验。从中摸索出一套最适的转化条件:感受态为对数生长初期(5.5小时),转化液中含有10~(-6)M的Ca~(2+),转化处理时间为60分钟。结果在灰色链霉菌中,用抗性标记直接转化获得成功,而且有65.9%的转化子已恢复了链霉素生物合成的能力。这就更进一步证明质粒与链霉素生物合成有关。

Plasmid pBR322 has been enlarged twice in vitro and the restriction enzyme mappings have been done for 4 newly constructed plasmids (pBW5,pBW7,pBW5-2 and pBW5 -8) by the related restriction endonucleases.With the aid of the analysis of agarose gel electrophoresis and the detection of selective markers of transforments,it was proved that the newly constructed plasmids are actually recombinants.All recombinant plasmids carry E.coli genes nrdA,B on which further studies can be carried out.Especially plasmids...

Plasmid pBR322 has been enlarged twice in vitro and the restriction enzyme mappings have been done for 4 newly constructed plasmids (pBW5,pBW7,pBW5-2 and pBW5 -8) by the related restriction endonucleases.With the aid of the analysis of agarose gel electrophoresis and the detection of selective markers of transforments,it was proved that the newly constructed plasmids are actually recombinants.All recombinant plasmids carry E.coli genes nrdA,B on which further studies can be carried out.Especially plasmids pBW5-2 and pBW5-8 supply a large amount of nrd A,B genes since they carry pKN402K DNA fragment.They greatly increase their copy number at temperatures above 35C during 2-3h,and contribute to the safe use of the plasmids due to that the great increase of their copy number is lethal to the host bacteria in rich media.

在体外对质粒pBR322进行了两次扩建,并作出新建四种质粒(pBW5,pBW7,pBW5-2和pBW5-8)的有关限制性核酸内切酶图谱。经琼脂糖凝胶电泳分析和对转化体选择标记检定,证明新建质粒确为重组质粒。重组质粒都携带有大肠杆菌nrdA,B基因,有利于对这些基因的深入研究。尤其是带有pKN402K DNA片段的新建质粒pBW5-2和pBW5-8在35℃以上2—3小时内拷贝数大量增加,这就为nrdA,B基因提供了丰富的材料;由于高温条件下,这种质粒拷贝数的扩增在丰富培养基里对宿主细菌是致死的,所以又为新建质粒的安全使用创造了条件。

The characterization of plasmids of three strains of S.griseus was studied by agarose gel electrophoresis analysis and E.M.observation.Our results have indicated that S.griseus No.45 (donor),a streptomycin producer,involves two plasmids,pSGl (19X106 dalton) and pSG2 (2.3X106 dalton),while the other,S.griseus No.45-3 (recipient),a plasmid-cured mutant with the loss of ability of streptomycin biosynthesis,still harbours pSG2; the third,S.griseus Tr.No.30 (trannsformant obtained by the pksmid DNA transformation...

The characterization of plasmids of three strains of S.griseus was studied by agarose gel electrophoresis analysis and E.M.observation.Our results have indicated that S.griseus No.45 (donor),a streptomycin producer,involves two plasmids,pSGl (19X106 dalton) and pSG2 (2.3X106 dalton),while the other,S.griseus No.45-3 (recipient),a plasmid-cured mutant with the loss of ability of streptomycin biosynthesis,still harbours pSG2; the third,S.griseus Tr.No.30 (trannsformant obtained by the pksmid DNA transformation using Lmr as a selective marker,regaines the ability of streptomycin synthesis and resumes the plasmid pSGl.This provides further evidence that there are two plasmids (pSGl and pSG2) present in S.griseus No.45 and it is pSGl not pSG2 that is closely related to the biosynthesis of streptomycin in this strain.

本文通过凝胶电泳分析以及电镜的观察,检测灰色链毒菌质粒DNA转化前后的给体、受体和转化子中质粒的存在情况,结合链霉素合成能力的变化证明,链霉素产生菌——灰色链霉菌No.45(给体)中存在两个质粒pSG1(19×10~6道尔顿)和pSG2(2.3×10~6道尔顿)。高温消除质粒的、丧失合成链霉素能力的、气生菌丝受抑制的突变体No.45—3(受体)中只有pSG2,说明高温漓除的质粒是pSG1。以结霉素抗性为筛选标记,通过质粒DNA转化而重新获得合成链霉素能力的转化子TrNo.30(转化子)中,既检测到pSG2,也检测到pSG1。这就进一步证明与链霉素生物合成有关的质粒是pSG1,而不是pSG2。上述电泳分析结果又为电镜观察所证实。

 
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