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ds rna
相关语句
  dsrna模板
     RAPID PREPARATION OF dsRNA TEMPLATE IN REVERSE TRANSCRIPTION(RT) POLYMERASE CHAIN REACTION(PCR)
     逆转录聚合酶链式反应中dsRNA模板的快速制备
短句来源
     A rapid, simple and reliable method to prepare dsRNA template for reverse transcription polymerase chain reaction is established.
     应用RT-PCR技术检测草鱼出血病病毒。 建立了一种快速、简易而可靠的dsRNA模板的制备方法。
短句来源
  双链rna
     This paper reports a new method based on double-stranded RNA (ds RNA) analysis for detection of apple latent viruses.
     本文报导一种以分析病毒双链RNA为依据,检测苹果潜隐性病毒的新方法。
短句来源
     This new approach permits rapid and effcient isolation and analysis of viral ds RNA from infected apple tissues, and, therefore, is potentialy useful for diagnosis of latent virus infections directly from the infected host tissues.
     这种方法能快速、准确地从苹果组织中分离和分析病毒的双链RNA(ds RNA),因此能直接、有效地从病毒侵染的组织诊断苹果潜隐性病毒。
短句来源
     Protein phosphorylation was found in the process of plant resistant response toward pathogens. It was found that MBP kinase, Phytophthora megasperma elicitor,syringomycin,pp29 and pp51 kinases and double strand RNA(ds RNA)were related to the phosphorylation events.
     在植物抗病反应中 ,发现有蛋白磷酸化现象发生 ,已经证明的有关因子包括 MBP蛋白激酶、大雄疫霉激发子、丁香霉素、pp2 9和 pp51蛋白激酶以及双链 RNA。
短句来源
  “ds rna”译为未确定词的双语例句
     Results Stimulation of BEAS-2B cells with dsRNA increased the mRNA levels of IL-8,GM-CSF,IL-1β,SAA,B factor and SLPI.
     结果BEAS-2B细胞在dsRNA刺激后,IL-8、GM-CSF、IL-1β、SAA、补体B因子、SLPI的mRNA均增高。
短句来源
     Methods BEAS-2B airway epithelial cells were treated with FP(10~(-7) mol·L~(-1)) for 2 h before stimulation with dsRNA(25 mg·L~(-1)).
     方法FP(10-7mol.L-1)作用于BEAS-2B 2 h后,加入dsRNA 25 mg.
短句来源
     Electrophoresis indicated that the total M. Wt(×10~6) of the ds RNA was estimated to be 16.66, with segments of 2.70, 2.30, 1.90, 1.70, 1.68, 1.50, 1.38, 1.20, 1.10, 0.60, 0.35 and 0.25. The dsRNA was infective when it was injected into Nephotettix cincticeps.
     用聚丙烯酰胺凝胶电泳,其RNA的总分子量为16.66×10~6d,含组分分子量为2.70、2.30、1.90、1.70、1.68、1.50、1.38、1.20、1.10、0.60、0.35和0.25(×10~6)。
短句来源
     This is the first report of the full-length cDNA clone and the complete sequence from the dsRNA genomic segment 3 (S3) of rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus of the family Reoviridae.
     报道了水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)基因组片段3(S3)的全序列。
短句来源
     At first, dsRNA expressing vector pP0491RNAi with 263bp-longed exon fragment of P0491E01 was constructed .
     首先从基因组DNA中克隆P0491E01一段263bp的外显子片段,构建dsRNA表达载体pP0491RNAi。
短句来源
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  相似匹配句对
     RNA Interference
     RNA干涉
短句来源
     RNA interference
     RNA干扰
短句来源
     DS RING
     DS环
短句来源
     The conditions are presented for the use of the ds RNA analysis for detection of apple latent viruses.
     文中还报导了使用这一技术诊断苹果潜隐性病毒的有关技术条件。
短句来源
     Analysis of Genomes of Rotavirus ds RNA Segments in Infantile Diarrhea by Gel Electrophoresis
     婴幼儿急性轮状病毒性腹泻的核酸凝胶电泳分析
短句来源
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  ds rna
Four distinct double-stranded (ds) RNA bands were extracted from leaves of Raphanus sativus-root cv.
      
The W8 isolate of the phytopathogenic fungus, Rosellinia necatrix that causes white root rot, contained three segments of double-stranded (ds) RNA, namely L1, L2 and M.
      
We investigated how the protein interacts with double-stranded (ds) RNA and other nucleic acids.
      
A double stranded (ds) RNA genome of Gremmeniella abietina mitochondrial RNA virus S1 (GaMRV-S1) was sequenced.
      
Two double stranded (ds) RNA molecule patterns, probably of viral origin, were sequenced from Gremmeniella abietina var.
      
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Fungal virus nucleic acids isolated from Penicillium chrysogenum 803, Penicillium chrysogenum 316 and Gaeumannomyces graminis have been detected by Ouchterlony doublediffusion test with antisera to Poly (Ⅰ): Poly (C) prepared previousely. This antisera can react with all these virus nucleic acids either extracted from virus particles or directly from fungal mycelia, and give visible precipitin line in double diffusion test, but not react with thermal denatured nucleic acids of these viruses, TMV-RNA and...

Fungal virus nucleic acids isolated from Penicillium chrysogenum 803, Penicillium chrysogenum 316 and Gaeumannomyces graminis have been detected by Ouchterlony doublediffusion test with antisera to Poly (Ⅰ): Poly (C) prepared previousely. This antisera can react with all these virus nucleic acids either extracted from virus particles or directly from fungal mycelia, and give visible precipitin line in double diffusion test, but not react with thermal denatured nucleic acids of these viruses, TMV-RNA and yeast RNA. The antisera to Poly (I): Poly (C) can also react with purified virions of Penicillium chrysogenum 803 and Penicillium chrysogenum 316 giving visible precipitin lines in doubls diffusion test, but not react with virions of Gaeumannomyces graminis. Consequently the antisera prepared to home-made Poly (I): Poly (C) can be used in screening fungal virusee with ds-RNA.

用国产多聚[I]:多聚[C]制备成双链RNA特异性的抗血清。在Ouchterlony免疫双扩散中此抗血清能和产黄青霉803,316及小麦全蚀病菌的病毒核酸产生沉淀线,而和106℃加热迅速冷却变性的上述病毒核酸以及酵母RNA,TMV-RNA均不产生沉淀线。证明三株真菌的病毒核酸为ds—RNA。试验也证明在免疫双扩散中,多聚[I]:多聚[C]抗血清和产黄青霉803,316的病毒体也可产生沉淀线,但和小麦全蚀病菌的病毒体不产生沉淀线。依同法也可直接测定由感染真菌病毒的菌丝体中提取的病毒ds-RNA。从而可用这一简易灵敏的免疫化学方法筛选双链RNA真菌病毒。

The antiserum specific to double-stranded ribouncleic acid has been pre- pared by immunizing rabits with complexes of synthetic double-stranded Polyinosinic: polycytidylic acid [Poly(Ⅰ): Poly(C)] produced bp Jiang Men Sugur Cane Chemical Factory in Guangzhou and methylated bovine serum albumin (MBSA). The titer of antiserum was 1/128 and 1/6400 by precipitin ring test with Poly (Ⅰ): Poly(C) and radioimmunoassay with ~3H-labeled ds-RNA respectively. The antiserum produced vi- sible precipitin line...

The antiserum specific to double-stranded ribouncleic acid has been pre- pared by immunizing rabits with complexes of synthetic double-stranded Polyinosinic: polycytidylic acid [Poly(Ⅰ): Poly(C)] produced bp Jiang Men Sugur Cane Chemical Factory in Guangzhou and methylated bovine serum albumin (MBSA). The titer of antiserum was 1/128 and 1/6400 by precipitin ring test with Poly (Ⅰ): Poly(C) and radioimmunoassay with ~3H-labeled ds-RNA respectively. The antiserum produced vi- sible precipitin line with as little as 390 and 12.2 ng/ml homologous antigen-Poly (Ⅰ): Poly(C) by two-dimensional immunodiffusion test and counterimmunoelectrophoresis respectively and 10 pg of Poly(Ⅰ): Poly(C) can be detected by radioimmunoassay. The antiserum specifically reacts with double-stranded RNA, but does not react with natural single-stranded RNA, TMV-RNA, yeast RNA and calf thymus DNA. Tests show that home-made Poly (Ⅰ): Poly(C) mixed with MBSA could produce in rabbits antibodies which specifically recognize ds-RNA and are effective reagents for im- munochemical detection of small amount of ds-RNA of reoviruses, fungal viruses and virus RF-RNA.

用国产多聚肌苷酸和多聚胞嘧啶核苷酸(简称多聚[I]:多聚[C])制备成双链核糖核酸特异性抗血清,用环状沉淀和~3H标记的番茄花叶病毒双链核糖核酸测得抗血清效价分别为1:128和1:6400。用免疫琼脂双扩散、对流免疫电泳和放射免疫测定可测定出多聚[I]:多聚[C]的最低浓度分别为391ng、12.2/ng和10pg/ml抗血清和酵母、TMV、PVX 的单链核糖核酸、小牛胸腺DNA不反应。试验证明用国产多聚[I]:多聚[C]制备的抗血清,可有效地用干双链核糖核酸的免疫化学鉴定。由于在免疫双扩散和对流免疫电泳中抗血清能和微量双链核糖核酸反应并产生可见沉淀线,从而有可能利用这两种简便灵敏的方法测定病毒的双链RNA,单链RNA病毒的RF型RNA,以及双链RNA真菌病毒的筛选。

The partial Purified preparations of Rice Ragged Stunt Virus (RRSV) from fresh diseased rice leaves could be obtained in the high yield by the combination of the techniques of clarification with CCI_4, precipitation with Polyethylene Glycol,differential centrifugations and linear sucrose gradient centrifugations. The preparations showed the maxium of the ultra-violet absorption spectra was at 260 nm and the value of the A_(260)/A_(280) ratio was 1.6. By Electron microscopy the RRSV particles showed spiked isometric...

The partial Purified preparations of Rice Ragged Stunt Virus (RRSV) from fresh diseased rice leaves could be obtained in the high yield by the combination of the techniques of clarification with CCI_4, precipitation with Polyethylene Glycol,differential centrifugations and linear sucrose gradient centrifugations. The preparations showed the maxium of the ultra-violet absorption spectra was at 260 nm and the value of the A_(260)/A_(280) ratio was 1.6. By Electron microscopy the RRSV particles showed spiked isometric morphology in diameter ranged 54—65 nm. Electrophoresis experiments have demonstrated that the RRSV contain 10 separated ds-RNA genomes. The sizes and electrophoresis pattern of 10 viral ds-RNA genomes were consistent with the results reported by S. Kawano et al. In the partial purified preparations of RRSV three kinds of other particles could be also observed. Two isometric particles were in diameter about 75nm and 35nm respectively. The third had filamentous appearance and the concentration of the filaments obviously increased with the steps of purification of RRSV The possible origins of these three particles were discussed.

应用比较简单的一次四氯化碳澄清,二次聚乙二酵浓缩沉淀,差速离心和20%—50%的蔗糖密度梯度离心,可以获得分离提纯效果较好的水稻齿叶病病毒(RRSV)制剂,在260nm处有最大吸收值,在A_(260)/A_(280)的比值为1.6。用醋酸铀负染方法,可以观察到RRSV为直径54nm—65nm的球状颗粒,具有底部较宽的突起,突起的高度为8—11nm,宽度约为22nm。病毒的核酸为双链RNA,共有十组分散的基因组,这一结果和四方报道的一致,在这一提纯病毒的制剂中,尚发现有其他三种大小不同的球状和线状颗粒,对它们的可能来源进行了讨论。

 
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