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carrier molecule
相关语句
  承载分子
     Design of a carrier molecule and high level expression of peptide antibiotics hPAB-β
     一个承载分子的设计与肽抗生素hPAB-β的高效表达
短句来源
     Application of carrier molecule PaP3.30 in fusion expression of small bioactive peptides
     承载分子PaP3.30在小分子活性肽融合表达中的应用
短句来源
     In our previous study, a sequence encoding a protein PaP3.30(16.7kD) selected out from Pseudomonas aeruginosa phage 3 (PaP3) was used as carrier molecule to coexpress with hPAB-p gene which is a beta peptide antibiotic isolated from human keratinocytes by our laboratory.
     本室曾用简并引物从人皮肤角质形成细胞中克隆到一人β型肽抗生素,称hPAB-β,并根据hPABβp的特性设计并筛选到了一个承载分子PaP3.30(16.7kD),利用该分子能使hPAB-β融合蛋白在大肠杆菌中的表达量达到菌体总蛋白的40%。
短句来源
     Objective:To screen and clone a carrier molecule for the expression of small bioactive peptides at high levels.
     目的 :筛选并克隆一个承载分子 ,研究其在肽抗生素及其他小分子活性肽融合表达中的应用。
短句来源
     Six selected peptides were also expressed by the level of 35%~44% total bacterial proteins when fused to carrier molecule, PaP3.30. Conclusion: The new carrier molecular, PaP3.30, is versatile in the expression of small bioactive peptides.
     结论 :所筛选的承载分子PaP3.30具有很强的融合表达小分子活性肽的能力 ,为建立高效表达和制备活性小肽的技术平台创造了条件
短句来源
  “carrier molecule”译为未确定词的双语例句
     Objective:Our overall goal is to develop a targeted carrier molecule that can carry multiple copies of the same or different ligands/reporters. The purpose of the present study was to demonstrate the receptor-binding properties of DTPA-Mannosyl-Dextran in vitro.
     第一章DTPA-甘露糖-葡聚糖体外靶向试验目的:通过一种钙离子依赖的受体结合反应,证实一种靶向分子载体N-双羟乙基氨基酸与二乙三胺五乙酸-甘露糖-葡聚糖(DTPA- Mannosyl-Dextran)的甘露糖受体靶向性能。 我们的最终目标是开发一种能够携带靶向物质的大分子载体。
短句来源
     Conclusion Expression plasmid pQE CH constructed from the designed carrier molecule and hPAB β expresses peptide antibiotics hPAB β at high level.
     结论 设计的承载蛋白分子能够实现肽抗生素的高效表达。
短句来源
     An ester activation method was employed to synthesize enrofloxacin (ENFX) carring protein BSA andOVA. The conjugates ENFX-BSA and ENFX-OVA were identified by UV and amino acids automation analysis instrument,and resulted in conjugates with 48 ENFX molecules per carrier molecule (BSA), Splenocytes from mice immunized withENFX-BSA were fused with SP2/0 myeloma cells, and hybridomas secreting antibodies against enrofloxacin were selectedand cloned.
     碳二亚胺法合成了2种恩诺沙星(enrofloxacin,ENFX)人工抗原ENFX-BSA和ENFX-OVA,用紫外扫描和分析载体蛋白的氨基酸组成等方法对人工抗原进行鉴定,ENFX与BSA的结合比约为48∶1。
短句来源
  相似匹配句对
     The Carrier
     浅谈催化反应中活性组分的载体
短句来源
     Progress in Studying on Liposome as An Carrier for Biological Macro-molecule
     脂质体作为生物大分子载体的研究进展
短句来源
     molecule-1?
     molecule-1?
短句来源
     Isospectral Molecule
     同谱分子
短句来源
     Identification of the carrier
     承运人识别的法律问题
短句来源
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  carrier molecule
The carrier molecule that transports dopamine (DA) into dopamine neurons by an electrogenic, Na+- and Cl--transport-coupled mechanism is known as the dopamine transporter (DAT).
      
Since the photoprobe is inert until photolysis, the probe-modified native fragment can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule.
      
Since the photoprobe is inert until photolysis, the synthetic peptide-probe can be readily purified by high-performance liquid chromatography before cross-linking to the carrier molecule.
      
Kinetic analysis of oxoglutarate transport inhibition by pyridoxal5'-phosphate indicated that modification of a single amino acid residue/carrier molecule wassufficient for complete inhibition of oxoglutarate transport.
      
This paper shows that the ion carrier molecule forms complexes of equivalent energy demands with Li+, Cs+, Na+, Rb+, and K+.
      
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Objective To design a carrier protein molecule which can express peptide antibiotics hPAB β at high level. Methods According to the properties of peptide antibiotics, a 489 bp DNA sequence coding for the carrier protein was designed under the help of DNAStar software. An expression plasmid pQE CH was constructed by inserting the fusion gene of designed DNA and peptide antibiotics hPAB β into pQE 32 plasmid. After the plasmid was transformed into E. coli JM109, 4 positive recombinants were selected...

Objective To design a carrier protein molecule which can express peptide antibiotics hPAB β at high level. Methods According to the properties of peptide antibiotics, a 489 bp DNA sequence coding for the carrier protein was designed under the help of DNAStar software. An expression plasmid pQE CH was constructed by inserting the fusion gene of designed DNA and peptide antibiotics hPAB β into pQE 32 plasmid. After the plasmid was transformed into E. coli JM109, 4 positive recombinants were selected and used to express the fusion proteins. Results All 4 selected recombinants containing the fusion gene of the designed carrier protein sequence and peptide antibiotics hPAB β expressed the fusion protein at high level about 40% to 45% of total cell proteins. Conclusion Expression plasmid pQE CH constructed from the designed carrier molecule and hPAB β expresses peptide antibiotics hPAB β at high level.

目的 通过设计一个承载蛋白分子高效表达肽抗生素hPAB β ,为大量制备hPAB β奠定基础。 方法 根据肽抗生素的特性 ,确立 4条设计原则 ,并据此设计一 489bp的承载蛋白编码序列 ,用化学合成法获得该序列并将之克隆到pQE 3 2中构建成表达载体 ,进一步将肽抗生素hPAB β基因插入上述表达载体 ,肽抗生素基因位于承载蛋白编码序列 3’ 端 ,两者构成融合基因。将含融合基因的表达载体转化大肠杆菌JM10 9,筛选阳性重组子 ,并用阳性重组子表达融合蛋白。结果 设计的承载蛋白为 163个氨基酸 ,分子量 17668.80 ,等电点 5 .62 1。用承载分子与肽抗生素hPAB β构建的融合蛋白表达载体转化细菌后 ,筛选到 4个阳性重组子 ,均能高效表达肽抗生素融合蛋白 ,融合蛋白的产量占细菌总蛋白的 40 %~ 45 %。结论 设计的承载蛋白分子能够实现肽抗生素的高效表达。

An ester activation method was employed to synthesize enrofloxacin (ENFX) carring protein BSA andOVA. The conjugates ENFX-BSA and ENFX-OVA were identified by UV and amino acids automation analysis instrument,and resulted in conjugates with 48 ENFX molecules per carrier molecule (BSA), Splenocytes from mice immunized withENFX-BSA were fused with SP2/0 myeloma cells, and hybridomas secreting antibodies against enrofloxacin were selectedand cloned. Two stable monoclonal antibodies, 2C5, 5D5 of subclasses...

An ester activation method was employed to synthesize enrofloxacin (ENFX) carring protein BSA andOVA. The conjugates ENFX-BSA and ENFX-OVA were identified by UV and amino acids automation analysis instrument,and resulted in conjugates with 48 ENFX molecules per carrier molecule (BSA), Splenocytes from mice immunized withENFX-BSA were fused with SP2/0 myeloma cells, and hybridomas secreting antibodies against enrofloxacin were selectedand cloned. Two stable monoclonal antibodies, 2C5, 5D5 of subclasses IgG2a, were isolated, Using 5D5, an indirect competitiveinhibition enzyme-linked immunosorbent assay (Ci-ELISA) was developed for the quantitative detection of enrofloxacinand its metabolites. The IC50 of standard curve was 21.67ngml-1 and the limit of detection for enrofloxacin was 0.13 ngml-1.This method was sensitive and had a linear range from 0.13 to 10 000 ngml-1 (r = -0.9782). Monoclonal antibodies 5D5exhibited high relative affinity to enrofloxacin, and the cross-reactivities with ciprofloxacin, marbofloxacin, sarafloxacin anddanorfloxacin were 110.8%, 27.40%, 71.05% and 37.41%, respectively. Three non-fluoroquinolones of cefadroxil,chloramphenicol, and sulfamonomethoxine were tested and there was no cross-reaction between them.

碳二亚胺法合成了2种恩诺沙星(enrofloxacin,ENFX)人工抗原ENFX-BSA和ENFX-OVA,用紫外扫描和分析载体蛋白的氨基酸组成等方法对人工抗原进行鉴定,ENFX与BSA的结合比约为48∶1。用ENFX-BSA免疫BALB/C小鼠,采用显微克隆技术最终筛选出2株特异性分泌细胞2C5和5D5并获取腹水,纯化后腹水效价分别为1∶3200和1∶6400。2C5和5D5均属IgG2a型抗体。单抗5D5间接竞争ELISA(Ci-ELISA)的工作浓度为1∶1000,ENFX的抑制曲线在0.13~10 000 ng·ml-1之间线性关系良好(r =-0.9782),检测限(LOD)为0.13 ng·ml-1,ENFX的IC50为21.67 ng·ml-1。单抗对氟喹诺酮类药物有很高的选择性,与环丙沙星、麻保沙星、沙拉沙星、达氟沙星的交叉反应率分别为110.84%、27.40%、71.05%和37.41%,而与头孢氨苄、氯霉素、磺胺间甲氧嘧啶等没有交叉反应。

Objective:To screen and clone a carrier molecule for the expression of small bioactive peptides at high levels. Methods: A carrier molecule, PaP3.30, was screened out from the genome of Pseudomonas aeruginosa phage PaP3 and its gene was cloned by PCR method and inserted into pQE 32 expression plasmid, this recombinant plasmid was named pQE PaP30. The peptide antibiotics hPAB β gene was then inserted into pQE PaP30 and induced to express the fusion protein in Escherichia coli . The ability...

Objective:To screen and clone a carrier molecule for the expression of small bioactive peptides at high levels. Methods: A carrier molecule, PaP3.30, was screened out from the genome of Pseudomonas aeruginosa phage PaP3 and its gene was cloned by PCR method and inserted into pQE 32 expression plasmid, this recombinant plasmid was named pQE PaP30. The peptide antibiotics hPAB β gene was then inserted into pQE PaP30 and induced to express the fusion protein in Escherichia coli . The ability of PaP3.30 to express other bioactive peptides was evaluated by fusing 6 different origins, varies in sizes and isoelectric points selected peptides to it. Results: After fused to PaP3.30, the peptide antibiotics hPAB β could express as fusion protein above 30% of total bacterial proteins. Six selected peptides were also expressed by the level of 35%~44% total bacterial proteins when fused to carrier molecule, PaP3.30. Conclusion: The new carrier molecular, PaP3.30, is versatile in the expression of small bioactive peptides.

目的 :筛选并克隆一个承载分子 ,研究其在肽抗生素及其他小分子活性肽融合表达中的应用。 方法 :根据肽抗生素为小分子活性肽 ,通常带正电荷的特性 ,从绿脓杆菌噬菌体 (PaP3)基因组中筛选一承载分子 ,构建其与肽抗生素hPAB β的融合蛋白表达载体 ,确认该承载分子能在大肠杆菌中融合表达肽抗生素后 ,再选择一组不同来源、不同大小、不同等电点的小肽分子作为研究对象 ,分别构建它们与承载分子的融合蛋白表达载体 ,转化大肠杆菌进行表达。根据表达情况 ,分析所选择的承载分子融合表达生物活性小肽的能力。 结果 :从PaP3基因组中成功地筛选到ORF 30编码的 1 6 2个氨基酸组成的蛋白作为承载分子 (PaP3.30 ) ;构建该承载分子与肽抗生素hPAB β的融合蛋白表达质粒pQE PaP30 hPAB β,在大肠杆菌中表达的融合蛋白占细菌总蛋白的 30 %以上 ;对所选择的不同来源 (人、蜜蜂、爪蟾、大肠杆菌 )、不同大小(相对分子质量为 70 0~5 30 0 )、不同等电点 (pⅠ 2 .9~ 1 2 )的六种小肽分子PaP3.30 ,均能实现其在大肠杆菌中高效融合表达 ,表达量为细菌总蛋白的 35 %~...

目的 :筛选并克隆一个承载分子 ,研究其在肽抗生素及其他小分子活性肽融合表达中的应用。 方法 :根据肽抗生素为小分子活性肽 ,通常带正电荷的特性 ,从绿脓杆菌噬菌体 (PaP3)基因组中筛选一承载分子 ,构建其与肽抗生素hPAB β的融合蛋白表达载体 ,确认该承载分子能在大肠杆菌中融合表达肽抗生素后 ,再选择一组不同来源、不同大小、不同等电点的小肽分子作为研究对象 ,分别构建它们与承载分子的融合蛋白表达载体 ,转化大肠杆菌进行表达。根据表达情况 ,分析所选择的承载分子融合表达生物活性小肽的能力。 结果 :从PaP3基因组中成功地筛选到ORF 30编码的 1 6 2个氨基酸组成的蛋白作为承载分子 (PaP3.30 ) ;构建该承载分子与肽抗生素hPAB β的融合蛋白表达质粒pQE PaP30 hPAB β,在大肠杆菌中表达的融合蛋白占细菌总蛋白的 30 %以上 ;对所选择的不同来源 (人、蜜蜂、爪蟾、大肠杆菌 )、不同大小(相对分子质量为 70 0~5 30 0 )、不同等电点 (pⅠ 2 .9~ 1 2 )的六种小肽分子PaP3.30 ,均能实现其在大肠杆菌中高效融合表达 ,表达量为细菌总蛋白的 35 %~4 4 %。 结论 :所筛选的承载分子PaP3.30具有很强的融合表达小分子活性肽的能力 ,为建立高效表达和制备活性小肽的技术平台创造了条件

 
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