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vegetative insecticidal protein     
相关语句
  营养期杀虫蛋白
     Novel vegetative insecticidal protein Vip3A is a non-crystal and extracellular toxic protein secreted outside the cell during Bacillus thuringiensis mid-logarithmic growth.
     营养期杀虫蛋白(Vegetative Insecticidal Protein,Vip)Vip3A是苏云金芽孢杆菌(Bacillus thuringiensis,Bt)在营养生长对数中期分泌产生的一类新型杀虫蛋白。
短句来源
     Then, three vegetative insecticidal protein gene vip83, vip14 and vip15 are obtained from the libraries through the methods of colony-blot-in-situ screening and enzyme-cut detection.
     通过菌落原位杂交筛选和酶切鉴定分别得到 3个相应的营养期杀虫蛋白基因vip83、vip14和vip15 ,并对其测序。
短句来源
     Studies on Cloning and Expression of Vegetative Insecticidal Protein from Bacillus Thuringiensis
     苏云金杆菌营养期杀虫蛋白基因的克隆与表达研究
短句来源
     Advances in Studies on Bacillus thuringiensis Novel Vegetative Insecticidal Protein Vip3A
     苏云金芽孢杆菌新型营养期杀虫蛋白Vip3A研究
短句来源
     Among the 23 strains of 20 serovars, the occurrence of vegetative insecticidal protein gene was 56.5% (13 positive in 23).
     在 2 0个不同血清型的 2 3种菌株中 ,营养期杀虫蛋白基因的存在率为 5 6 5 % (13 2 3) .
短句来源
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  营养期杀虫蛋白基因
     Then, three vegetative insecticidal protein gene vip83, vip14 and vip15 are obtained from the libraries through the methods of colony-blot-in-situ screening and enzyme-cut detection.
     通过菌落原位杂交筛选和酶切鉴定分别得到 3个相应的营养期杀虫蛋白基因vip83、vip14和vip15 ,并对其测序。
短句来源
     Studies on Cloning and Expression of Vegetative Insecticidal Protein from Bacillus Thuringiensis
     苏云金杆菌营养期杀虫蛋白基因的克隆与表达研究
短句来源
     Characteristic Analysis of Vegetative Insecticidal Protein Gene from Bacillus thuringiensis
     苏云金芽胞杆菌营养期杀虫蛋白基因特性分析
短句来源
     Using Southern blot of gene localization, vegetative insecticidal protein gene was determined neither on chromosome nor circle plasmids.
     通过Southern杂交的基因定位实验 ,证实了营养期杀虫蛋白基因并非位于染色体和环型质粒上 ;
短句来源
     In the conclusion, these results indidate that vegetative insecticidal protein gene is highly conserved and is distributed broader in the natural strains of Bacillus thuringiensis, and the specific structure of its coded sequence implied potential function,which contributed to further making vegetative insecticidal protein clear.
     上述结果提示 ,营养期杀虫蛋白基因在苏云金芽胞杆菌天然菌株中高度保守并较为广泛分布 ,其编码蛋白的特殊结构提示了营养期杀虫蛋白潜在的功能特性 ,为进一步研究营养期杀虫蛋白奠定了基础 .
短句来源
更多       
  营养期杀虫蛋白
     Novel vegetative insecticidal protein Vip3A is a non-crystal and extracellular toxic protein secreted outside the cell during Bacillus thuringiensis mid-logarithmic growth.
     营养期杀虫蛋白(Vegetative Insecticidal Protein,Vip)Vip3A是苏云金芽孢杆菌(Bacillus thuringiensis,Bt)在营养生长对数中期分泌产生的一类新型杀虫蛋白。
短句来源
     Then, three vegetative insecticidal protein gene vip83, vip14 and vip15 are obtained from the libraries through the methods of colony-blot-in-situ screening and enzyme-cut detection.
     通过菌落原位杂交筛选和酶切鉴定分别得到 3个相应的营养期杀虫蛋白基因vip83、vip14和vip15 ,并对其测序。
短句来源
     Studies on Cloning and Expression of Vegetative Insecticidal Protein from Bacillus Thuringiensis
     苏云金杆菌营养期杀虫蛋白基因的克隆与表达研究
短句来源
     Advances in Studies on Bacillus thuringiensis Novel Vegetative Insecticidal Protein Vip3A
     苏云金芽孢杆菌新型营养期杀虫蛋白Vip3A研究
短句来源
     Among the 23 strains of 20 serovars, the occurrence of vegetative insecticidal protein gene was 56.5% (13 positive in 23).
     在 2 0个不同血清型的 2 3种菌株中 ,营养期杀虫蛋白基因的存在率为 5 6 5 % (13 2 3) .
短句来源
更多       
  营养期杀虫蛋白基因
     Then, three vegetative insecticidal protein gene vip83, vip14 and vip15 are obtained from the libraries through the methods of colony-blot-in-situ screening and enzyme-cut detection.
     通过菌落原位杂交筛选和酶切鉴定分别得到 3个相应的营养期杀虫蛋白基因vip83、vip14和vip15 ,并对其测序。
短句来源
     Studies on Cloning and Expression of Vegetative Insecticidal Protein from Bacillus Thuringiensis
     苏云金杆菌营养期杀虫蛋白基因的克隆与表达研究
短句来源
     Characteristic Analysis of Vegetative Insecticidal Protein Gene from Bacillus thuringiensis
     苏云金芽胞杆菌营养期杀虫蛋白基因特性分析
短句来源
     Using Southern blot of gene localization, vegetative insecticidal protein gene was determined neither on chromosome nor circle plasmids.
     通过Southern杂交的基因定位实验 ,证实了营养期杀虫蛋白基因并非位于染色体和环型质粒上 ;
短句来源
     In the conclusion, these results indidate that vegetative insecticidal protein gene is highly conserved and is distributed broader in the natural strains of Bacillus thuringiensis, and the specific structure of its coded sequence implied potential function,which contributed to further making vegetative insecticidal protein clear.
     上述结果提示 ,营养期杀虫蛋白基因在苏云金芽胞杆菌天然菌株中高度保守并较为广泛分布 ,其编码蛋白的特殊结构提示了营养期杀虫蛋白潜在的功能特性 ,为进一步研究营养期杀虫蛋白奠定了基础 .
短句来源
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      vegetative insecticidal protein
    The diversity of Bt strains facilitates isolation of new types of cry and vip (vegetative insecticidal protein) genes.
          
    Vegetative insecticidal protein (Vip), a unique class of insecticidal protein, is now part of transgenic plants for conferring resistance against lepidopteron pests.
          
    The metabolic power-times curves of Bacillus thuringiensis and its vegetative insecticidal protein-engineered strains were determined at 30°C using a thermal activity monitor, air Isothermal Microcalorimeter, and ampoule method.
          
    Studies on the growth metabolism of Bacillus thuringiensis and its vegetative insecticidal protein engineered strains by microca
          
    Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects.
          
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    Three kinds of Bacillus thuringiensis serotype-subsp. Leesis(H 33) strain YBT-833, subsp. Aizawai(H 7) strain YBT-1416 and subsp. Kurstaki(H 3ab) strain YBT-1535, which were isolated by our lab, are chosen as original strain to clone vegetative insecticidal protein gene. Southern hybridization showed that vip genes are all localized at roughly 4~5 kb size-fractionated XbaI fragments of total DNA from YBT-833, YBT-1416 and YBT-1535. Three subgenomic libraries containing the vip gene fragment, were...

    Three kinds of Bacillus thuringiensis serotype-subsp. Leesis(H 33) strain YBT-833, subsp. Aizawai(H 7) strain YBT-1416 and subsp. Kurstaki(H 3ab) strain YBT-1535, which were isolated by our lab, are chosen as original strain to clone vegetative insecticidal protein gene. Southern hybridization showed that vip genes are all localized at roughly 4~5 kb size-fractionated XbaI fragments of total DNA from YBT-833, YBT-1416 and YBT-1535. Three subgenomic libraries containing the vip gene fragment, were constructed with pUC19 as vector. Then, three vegetative insecticidal protein gene vip83, vip14 and vip15 are obtained from the libraries through the methods of colony-blot-in-situ screening and enzyme-cut detection. Comparision of DNA sequence made out that only vip83 gene exist five different base pairs with known vip genes. Because the sequences of vip14 and vip15 are the same, two of the three genes, vip83 and vip14, were subcloned to shuttle vehicle pHT315 to get recombinant plasmids pBMB8901 and pBMB8902 in turn. The plasmids were separately transformed into vip - Bt. receptors BMB171 and 4Q7 to obtain four engineered strains BMB8901-171, BMB8902-171, BMB8901-4Q7 and BMB8902-4Q7. SDS-PAGE results indicated that all recombinant strains express 88kD vegetative insecticidal protein. Bioassay also showed that the proteins of genes vip83 and vip14 both have certain toxicity to Lepidopteran insect larvae such as Heliochis armigera, Spodotera exigua and Plutella xylostella. While the toxicity of vip protein from four engineered strains to Plutella xylostellas are highest , whose LC 50 value is 28.6, 31.6, 45.4 and 37.6μL/mL respetively. This study will contributed to construct high efficacy and wide spectrum engineered strains on theory and reality.

    选择本实验室分离的苏云金芽胞杆菌李氏亚种 (subsp .Leesis)菌株YBT 833、鲇泽亚种 (subsp .Aizawai)菌株YBT 1416和库斯塔克亚种 (subsp .Kurstaki)菌株YBT 15 35为出发菌株 ,以营养期杀虫蛋白基因PCR扩增的特异片段为探针 ,进行总DNA酶切片段的Southern杂交定位。结果显示 3株菌株的营养期杀虫蛋白基因 ,均位于经XbaI完全消化的 4~ 5kb大小的DNA片段上。将该区域DNA片段回收后克隆到pUC19载体 ,建立了 3个较基因组文库小的亚基因组文库。通过菌落原位杂交筛选和酶切鉴定分别得到 3个相应的营养期杀虫蛋白基因vip83、vip14和vip15 ,并对其测序。DNA序列比较发现基因vip83与已知营养期杀虫蛋白基因存在 5个差异碱基。将vip83、vip14基因亚克隆到苏云金芽胞杆菌 大肠杆菌穿梭载体pHT315 ,分别得到重组质粒pBMB890 1和pBMB890 2。将它们电转化到vip- 的B .t.受体菌BMB171和 4Q7,获得了相应的工程菌BMB890 1 171,BMB890 2 171,BMB890 1 4Q...

    选择本实验室分离的苏云金芽胞杆菌李氏亚种 (subsp .Leesis)菌株YBT 833、鲇泽亚种 (subsp .Aizawai)菌株YBT 1416和库斯塔克亚种 (subsp .Kurstaki)菌株YBT 15 35为出发菌株 ,以营养期杀虫蛋白基因PCR扩增的特异片段为探针 ,进行总DNA酶切片段的Southern杂交定位。结果显示 3株菌株的营养期杀虫蛋白基因 ,均位于经XbaI完全消化的 4~ 5kb大小的DNA片段上。将该区域DNA片段回收后克隆到pUC19载体 ,建立了 3个较基因组文库小的亚基因组文库。通过菌落原位杂交筛选和酶切鉴定分别得到 3个相应的营养期杀虫蛋白基因vip83、vip14和vip15 ,并对其测序。DNA序列比较发现基因vip83与已知营养期杀虫蛋白基因存在 5个差异碱基。将vip83、vip14基因亚克隆到苏云金芽胞杆菌 大肠杆菌穿梭载体pHT315 ,分别得到重组质粒pBMB890 1和pBMB890 2。将它们电转化到vip- 的B .t.受体菌BMB171和 4Q7,获得了相应的工程菌BMB890 1 171,BMB890 2 171,BMB890 1 4Q7和BMB890 2 4Q7。SDS PAGE电泳检测均有 88kD大小的蛋白表达。生物测定结果亦表明了 ,营养期杀虫蛋白Vip83和Vip14对鳞翅目棉铃虫、小菜蛾和甜菜夜蛾的三龄幼虫均有一定的杀虫活性 ;其中对小菜蛾的毒力最高 ,LC50 值分别为 2 8.6 ,31.6 ,45 .4和 37.6 μL mL。该结?

    Novel vegetative insecticidal proteins (VIPs) are secreted outside the cell during Bacillus thuringiensis(Bt) mid-logarithmic growth. Bt harbors a variety of VIPs, including VIP1,VIP2 and VIP3. VIP1 and VIP2 are components of a binary toxin which is specifically active against diabrotica insect larvae. However, the mechanism of this specific activity isn't known. VIP3 shows activity against many lepidopteran insect larvae. It exerts insecticidal properties on susceptible insect cells by triggering...

    Novel vegetative insecticidal proteins (VIPs) are secreted outside the cell during Bacillus thuringiensis(Bt) mid-logarithmic growth. Bt harbors a variety of VIPs, including VIP1,VIP2 and VIP3. VIP1 and VIP2 are components of a binary toxin which is specifically active against diabrotica insect larvae. However, the mechanism of this specific activity isn't known. VIP3 shows activity against many lepidopteran insect larvae. It exerts insecticidal properties on susceptible insect cells by triggering an apoptotic-type of programmed cell death(PCD). This mechanism is different from that of insecticidal crystal proteins(ICPs), and can be used to screen for novel chemical ligands. Recently vip genes have been applied in the construcion of transgenic plants which are resistant to insects, and maize plants active against insects were obtained. The potential prospect on chimeric toxins derived from VIPs and host microorganisms transformed with vip or its hybrid genes was discussed.

    营养期杀虫蛋白 (vegetativeinsecticidalproteins ,VIPs)是苏云金杆菌 (Bacillusthuringiensis,Bt)在对数生长中期分泌的一类新型杀虫毒蛋白。VIPs主要分为VIP1、VIP2和VIP3三种。VIP1和VIP2构成二元毒素 ,对鞘翅目叶甲科的昆虫具有杀虫特异性 ;而VIP3对鳞翅目昆虫具有较广谱的杀虫活性。VIP1和VIP2的杀虫作用机理还不清楚 ;VIP3通过诱发细胞凋亡 ,最终导致昆虫死亡 ,这种作用机理与Bt杀虫晶体蛋白的作用机理完全不同 ,这为筛选新的杀虫活性物质提供了新的思路。vip基因现已被应用于转基因杀虫植物的构建 ,得到高效抗虫的多价转基因玉米。此外 ,VIPs嵌合蛋白的构建、vip及其融合基因导入其它许多宿主微生物等方面的研究也具有诱人的潜在应用前景。

    The discovery, characteristics, functional mode, mechanism, and advance in study of vegetative insecticidal proteins (VIPs), a new type of insecticidal proteins as well as the encoding genes, which were found, extracted, and purified from Bacillus strains were briefly introduced in this paper. Moreover, the insecticidal activity and safety of VIPs was evaluated and indicated.\;

    简述了芽孢杆菌属 (Bacillus)中的新型活性杀虫蛋白 营养期杀虫蛋白VIPs及其编码基因的发现、特性、作用模式及机制等研究进展 ,并对其杀虫活性和安全因素作了初步评估和预示。

     
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