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woody fruit crops
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  木本果树
     A method for genomic DNA preparation of woody fruit crops
     一种木本果树基因组DNA提取方法研究
短句来源
     Advances in Research on Genetic Transformation in Woody Fruit Crops
     木本果树遗传转化研究进展
短句来源
     This article presents an overview of the progress in genetic transformation of woody fruit crops,particular attention is given to the methods of transformation and the factors affecting gene transfer.
     从遗传转化方法和影响遗传转化的因素两方面综述了木本果树遗传转化技术近年来的进展。
短句来源
  “woody fruit crops”译为未确定词的双语例句
     1. A simple and efficient method for genomic DNA extraction from woody fruit crops containing high level of polysaccharides was developed.
     1、完善了柑橘类作物的DNA提取方法。 发现在DNA提取过程中高浓度NaCl结合水饱和乙醚处理能有效去除多糖,获得高纯度的DNA样品。
短句来源
     Understanding the processes regulating the phase transition from vegetative to reproductive growth is particularly important for woody fruit crops.
     了解植物由营养生长向生殖生长的转变的调节过程,对于控制有花植物的生长发育是很有意义的。
短句来源
     Extraction of genomic DNA from woody fruit crops has been modified based on the rice genomic DNA extraction procedure.
     研究了一种可有效排除细胞内多酚类等杂质污染的简便、快捷、经济的果树基因组 DNA提取方法。
  相似匹配句对
     Fruit
     水果
短句来源
     Advances in Research on Genetic Transformation in Woody Fruit Crops
     木本果树遗传转化研究进展
短句来源
     vegetables, fruit &.
     (2)果蔬最少加工;
     A method for genomic DNA preparation of woody fruit crops
     一种木本果树基因组DNA提取方法研究
短句来源
     The Wild Woody Fruit Tree Resources in the Coastland of Mount Laoshan, Qingdao
     青岛崂山海岸带野生木本果树资源
短句来源
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  woody fruit crops
Development and application of molecular marker linkage maps in woody fruit crops
      
We describe a simple and efficient method for genomic DNA extraction from woody fruit crops containing high polysaccharide levels.
      


Extraction of genomic DNA from woody fruit crops has been modified based on the rice genomic DNA extraction procedure The modification is to remove most of the cytoplasmic components prior to lysis of the nuclei by SDS and to inhibit the formation of oxidized polyphenolic compounds With the modified method, the genomic DNA samples prepared from citrus, apple, pear, crabapple, peach, cherry and loquat are susceptible to digestion with restriction endonucleases and ready to be used as templates for polymerase...

Extraction of genomic DNA from woody fruit crops has been modified based on the rice genomic DNA extraction procedure The modification is to remove most of the cytoplasmic components prior to lysis of the nuclei by SDS and to inhibit the formation of oxidized polyphenolic compounds With the modified method, the genomic DNA samples prepared from citrus, apple, pear, crabapple, peach, cherry and loquat are susceptible to digestion with restriction endonucleases and ready to be used as templates for polymerase chain reaction (PCR)

研究了一种可有效排除细胞内多酚类等杂质污染的简便、快捷、经济的果树基因组DNA提取方法.其改良策略是:在细胞核被裂解前去除细胞质中的多酚类等杂质,防止多酚类物质被氧化,从而排除多酚类等杂质的干扰.利用这一方法提取的柑桔、枇杷、梨、苹果、湖北海棠、桃、樱桃等7种果树基因组DNA均能被限制性内切酶完全消化,获得理想的Southern杂交结果,也可作为PCR模板应用,成功地进行特异性基因扩增和RAPD.

This article presents an overview of the progress in genetic transformation of woody fruit crops,particular attention is given to the methods of transformation and the factors affecting gene transfer.Agrobacterium-mediated and particle biolistics are the main methods used in genetic transformation of fruit trees.The factors affecting gene transfer are discussed from several aspects,such as plant targets and its regeneration system,marker gene and the selection of transformed cells and the conditions...

This article presents an overview of the progress in genetic transformation of woody fruit crops,particular attention is given to the methods of transformation and the factors affecting gene transfer.Agrobacterium-mediated and particle biolistics are the main methods used in genetic transformation of fruit trees.The factors affecting gene transfer are discussed from several aspects,such as plant targets and its regeneration system,marker gene and the selection of transformed cells and the conditions of culture.Papers reporting the introduction of target genes into woody fruit crops have grouped into one table.The article also discusses the development of genetic engineering of woody fruit crops.

从遗传转化方法和影响遗传转化的因素两方面综述了木本果树遗传转化技术近年来的进展。木本果树遗传转化方法主要有农杆菌介导法和基因枪法;影响转化的因素主要从受体与再生系统、选择标记基因与转化细胞的筛选、培养方法与培养条件等方面进行论述。文章还归纳了导入目的基因并获得再生植株的木本果树种类,并对木本果树基因工程进展及展望进行了讨论。

>=Extraction of genomic DNA from woody fruit crops has been modified based on the rice genomic DNA extraction procedure. The modification is to remove most of the cytoplasmic components prior to lysis of the nuclei by SDS and to inhibit the formation of oxidized polyphenolic compounds. With the modified method,the genomic DNA samples prepared from citrus,apple,pear,crabapple, peach, cherry and loquat are susceptible to digestion with restriction endonucleases, satisfied the experiment of southern blots,...

>=Extraction of genomic DNA from woody fruit crops has been modified based on the rice genomic DNA extraction procedure. The modification is to remove most of the cytoplasmic components prior to lysis of the nuclei by SDS and to inhibit the formation of oxidized polyphenolic compounds. With the modified method,the genomic DNA samples prepared from citrus,apple,pear,crabapple, peach, cherry and loquat are susceptible to digestion with restriction endonucleases, satisfied the experiment of southern blots, and ready to be used as templates for polymerase chain reaction(PCR)and RAPD.

研究了一种可有效排除细胞内多酚类等杂质污染的简便、快捷、经济的果树基因组 DNA提取方法。其改良策略是:在细胞核被裂解前去除细胞质中的多酚类等杂质,防止多酚类物质被氧化,从而排除多酚类等杂质的干扰。利用这一方法提取的柑橘、枇杷、梨、苹果、湖北海棠、桃、樱桃等7种果树基因组DNA均能被限制性内切酶完全消化,获得理想的Southern杂交结果,也可作为PCR模板应用,成功地进行特异性基因扩增和RAPD。

 
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