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cis retinoic acid
相关语句
  顺式维甲酸
    9 cis retinoic acid induces apoptosis in HL 60 cells
    9-顺式维甲酸诱导HL-60细胞凋亡
短句来源
    Objective:To investigate the apoptosis of HL 60 cells induced by 9 cis retinoic acid (9 cis RA),and illustrate the possible molecular mechanism.
    目的:检测9-顺式维甲酸(9-cisRA)诱导HL-60细胞凋亡的能力,并探讨其分子机制。
短句来源
    Objective To study the effects of vitamin D 3(VD 3), 9 cis retinoic acid (9 cis RA)and their receptors on the regulation of human hsp90β gene.
    目的 研究维生素 D3(VD3)、9-顺式维甲酸 (9- cis- RA)及其相应核受体对 hsp90 β基因表达的调控作用。
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  cis retinoic acid
The impact of pretransplant cytoreductive treatment, such as intensive chemotherapy, splenectomy, or 13-cis retinoic acid, is unclear.
      
However, 13-cis retinoic acid (RA) and SAHA, a histone deacetylase inhibitor, have each been shown to induce apoptosis in medulloblastoma cultures and mouse models.
      
Response of preclinical medulloblastoma models to combination therapy with 13-cis retinoic acid and suberoylanilide hydroxamic a
      
According to in vitro and in vivo clinical studies, 13-cis retinoic acid (cRA) may be a promising agent for maintenance therapy in these patients.
      
In view of these results, 9-cis retinoic acid or stable analogues of this retinoid may have potential for the treatment of neuroblastoma.
      
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Changes of cell cycle kinetics of HL-60 cell line after ALL-trans retinoic acid(ATRA)and 9-cis retinoic acid(9-cis RA)induction were studied with BrdU/DNA bivariated flow cytometry. HL-60 cells were exposed to ATRA or 9-cis RA for 96 h. During the exposure, the RA-treated cells were obtained at 24,48,72 and 96 h and then the cell cycle kinetics was meas ured with BrdU/DNA bivariated flow cytometry.Data from 4 parameters of cell cycle showed that treatment with ATRA or 9-cis RA for 96...

Changes of cell cycle kinetics of HL-60 cell line after ALL-trans retinoic acid(ATRA)and 9-cis retinoic acid(9-cis RA)induction were studied with BrdU/DNA bivariated flow cytometry. HL-60 cells were exposed to ATRA or 9-cis RA for 96 h. During the exposure, the RA-treated cells were obtained at 24,48,72 and 96 h and then the cell cycle kinetics was meas ured with BrdU/DNA bivariated flow cytometry.Data from 4 parameters of cell cycle showed that treatment with ATRA or 9-cis RA for 96 h resulted in a decrease in the cell BrdU LI,production rate and an increase in Ts and Tpot. Mean while the ability of the cell to reduce NBT was enhanced by RA induction, and the percentage of matured cells was increased by cell morphology assessment.These results suggested that both ATRA and 9-cis-RA may induce HL-60 cell differentiation.

用Brdu/DNA双参数流式细胞仪技术研究了HL-60细胞经全反式维甲酸(ATRA)和9-顺式维甲酸(9-cisRA)诱导24,48和96h后,细胞周期动力学各参数,BrdULI,Ts,Tpot和PR的变化,同时测定细胞NBT还原试验和细胞形态学的变化,发现随着维甲酸诱导时间的延长,BrdULI逐渐降低,PR亦是逐渐减少,而Ts和Tpot则逐渐延长。NBT还原试验结果表明NBT阳性细胞百分比呈现上升趋势:细胞形态学观察显示稍成熟阶段细胞百分比上升,早幼粒阶段细胞所占百分比下降。本文观察了ATRA和9-cisRA在诱导HL-60细胞过程中以上各参数的变化,结果提示两类维甲酸对HL-60细胞均有诱导分化功能。

Objective:To investigate the apoptosis of HL 60 cells induced by 9 cis retinoic acid (9 cis RA),and illustrate the possible molecular mechanism.Methods:Apoptosis was detected by morphological observation,DNA electrophoresis and flow cytometric cell cycle analysis.Bcl 2 expression was detected by flow cytometry.Results:9 cis RA initiated apoptosis of HL 60 cells after inducing them irreversibly commited to differentiation.Bcl 2 was downregulated during the differentiation and apoptosis...

Objective:To investigate the apoptosis of HL 60 cells induced by 9 cis retinoic acid (9 cis RA),and illustrate the possible molecular mechanism.Methods:Apoptosis was detected by morphological observation,DNA electrophoresis and flow cytometric cell cycle analysis.Bcl 2 expression was detected by flow cytometry.Results:9 cis RA initiated apoptosis of HL 60 cells after inducing them irreversibly commited to differentiation.Bcl 2 was downregulated during the differentiation and apoptosis process.9 cis RA was more potent than all trans retinoic acid (ATRA) did in inducing terminal differentiation associated apoptosis and in downregulation of Bcl 2 expression. Conclusion:9 cis RA can induce apoptosis in HL 60 cells.Downregulation of Bcl 2 expression appears to play an important role in the apoptosis of the differentiated leukemic cells.

目的:检测9-顺式维甲酸(9-cisRA)诱导HL-60细胞凋亡的能力,并探讨其分子机制。方法:应用形态学观察、DNA电泳、流式细胞仪分析检测细胞凋亡,应用流式细胞仪检测HL-60细胞bcl-2含量。结果:9-cisRA可以诱导HL-60细胞在分化后发生典型的凋亡。在HL-60细胞分化和凋亡的过程中bcl-2含量逐渐下降。9-cisRA诱导HL-60细胞凋亡能力和降低bcl-2表达的能力均显著强于全反式维甲酸(A-TRA)。结论:9-cisRA具有诱导HL-60细胞凋亡的作用。bcl-2表达水平的降低可能是经诱导分化成熟的白血病细胞走向凋亡的重要条件。

Objective To study the effects of vitamin D 3(VD 3), 9 cis retinoic acid (9 cis RA)and their receptors on the regulation of human hsp90β gene. Methods We first transfected Jurkat cells with “full length”(-1 039 bp/+1 531 bp)hsp90β reporter plasmid β1.11,then the transfected cells were treated with VD 3 or/and 9 cis RA;or β1.11 was cotransfected with wild type vitamin D 3 receptor (VDR) or/and retinoid X receptor (RXR) expression construction and the reporter activities...

Objective To study the effects of vitamin D 3(VD 3), 9 cis retinoic acid (9 cis RA)and their receptors on the regulation of human hsp90β gene. Methods We first transfected Jurkat cells with “full length”(-1 039 bp/+1 531 bp)hsp90β reporter plasmid β1.11,then the transfected cells were treated with VD 3 or/and 9 cis RA;or β1.11 was cotransfected with wild type vitamin D 3 receptor (VDR) or/and retinoid X receptor (RXR) expression construction and the reporter activities were assayed.Western blot was carried out to detect the protein level of hsp90β in the Jurkat cells that were transfected by VDR cDNA or treated with VD 3 or/and 9 cis RA.By electrophoresis mobility shift assay (EMSA),we evaluated the DNA binding activity of VDR and RXR in Jurkat cell nuclear extracts. Results With VD 3 or/and 9 cis RA treatment,the expression of hsp90β gene was slightly increased.The induction became prominent when both of the ligands were present. The unliganded VDR or RXRα all slightly repressed the constitutive expression of hsp90β gene,while overexpression of VDR and RXRα further inhibited the gene.In addition,EMSA results showed that unliganded VDR and RXR simultaneously bound to the vitamin D 3 response element (VDRE) of hsp90β gene. Conclusions These results suggest that VD 3/VDR and 9 cis RA/RXR coparticipate in the regulation of hsp90β gene in Jurkat cells.

目的 研究维生素 D3(VD3)、9-顺式维甲酸 (9- cis- RA)及其相应核受体对 hsp90 β基因表达的调控作用。方法 采用 DEAE- Dextran方法将人 hsp90 β基因调控片段 (- 10 39bp/ +15 31bp)介导的荧光素酶 (L uc)报告基因质粒 β1.11转染 Jurkat细胞 ,并用 VD3和 9- cis- RA分别或同时刺激细胞 ,或将质粒 β1.11及野生型维生素D3受体 (VDR)或 /和维甲酸 X受体 α(RXRα)真核表达质粒共转染 Jurkat细胞 ,检测细胞裂解液中荧光素酶活性 ;用 Western印迹分析方法检测经 VDR真核表达质粒转染或用 VD3和 9- cis- RA刺激的 Jurkat细胞中内源性Hsp90β蛋白的改变 ;通过电泳迁移率变更分析 (EMSA)实验 ,检测 VDR和 RXR能否与含有 hsp90β基因第一内含子中维生素 D3应答元件 (i VDRE)的双链寡核苷酸片段发生特异结合。结果  VD3和 9- cis- RA可分别低水平诱导hsp90β基因表达 ,而两种配体同时加入有协同作用。 VDR或 RXRα分别转染只能较弱地抑制...

目的 研究维生素 D3(VD3)、9-顺式维甲酸 (9- cis- RA)及其相应核受体对 hsp90 β基因表达的调控作用。方法 采用 DEAE- Dextran方法将人 hsp90 β基因调控片段 (- 10 39bp/ +15 31bp)介导的荧光素酶 (L uc)报告基因质粒 β1.11转染 Jurkat细胞 ,并用 VD3和 9- cis- RA分别或同时刺激细胞 ,或将质粒 β1.11及野生型维生素D3受体 (VDR)或 /和维甲酸 X受体 α(RXRα)真核表达质粒共转染 Jurkat细胞 ,检测细胞裂解液中荧光素酶活性 ;用 Western印迹分析方法检测经 VDR真核表达质粒转染或用 VD3和 9- cis- RA刺激的 Jurkat细胞中内源性Hsp90β蛋白的改变 ;通过电泳迁移率变更分析 (EMSA)实验 ,检测 VDR和 RXR能否与含有 hsp90β基因第一内含子中维生素 D3应答元件 (i VDRE)的双链寡核苷酸片段发生特异结合。结果  VD3和 9- cis- RA可分别低水平诱导hsp90β基因表达 ,而两种配体同时加入有协同作用。 VDR或 RXRα分别转染只能较弱地抑制 hsp90β基因表达 ,二者共转染能增强其抑制作用。 EMSA实验证实 ,VDR和 RXR都可特异结合于 i VDRE上。结论  VD3和 9- cis- RA这两条信号途径共同参与 hsp90β基因的启动子活性调节。

 
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