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wild soybean glycine soja
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     ) and wild soybean (G. soja (L.)
     max×G.
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     and wild soybean ( dycine soja (L.)
     和野大豆(Glycine soja(L.)
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     Investigaton on Embryology of Wild Soybean
     野生大豆胚胎学研究
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     semi-wild soybean (G. gracilis skv.)
     、中间型大豆(G.gracilis skv.)
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     Effect of Temperature on Germinnation of Wild Soybean
     温度对野生大豆种子萌发的影响
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  wild soybean glycine soja
Lack of specific binding of labeledRhizobium japonicum lipopolysaccharides to wild soybean (Glycine soja) roots
      
A Preliminary Comparative Evaluation of Genetic Diversity between Chinese and Japanese Wild Soybean (Glycine soja) Germplasm Poo
      
The wild soybean (Glycine soja Sieb et Zucc.) QT2 lacks all the subunits of β-conglycinin, and this deficiency is controlled by a single dominant gene Scg-1 (suppressor of β-conglycinin).
      
Genetic variation in South Korean natural populations of wild soybean (Glycine soja)
      
Genetic regulation of linolenic acid concentration in wild soybean Glycine soja accessions
      
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There are two types of fast-growing Rhizobia isolated from nodules of wild Soybean(Glycine soja)growing in Liaoning Province on the Simmon's medium, in which 7 out of 12 strains didn't grow and the other 5 grew well, but their rates were slower than that of A, tumefaciens. All strains didn't produce 3-keto-1actose from lactose oxidation. Three strains showed acid reactions and eight strains showed alkaline reactions in litmus milk; but one strain (083) wasn't distinguishable. All fast-growing...

There are two types of fast-growing Rhizobia isolated from nodules of wild Soybean(Glycine soja)growing in Liaoning Province on the Simmon's medium, in which 7 out of 12 strains didn't grow and the other 5 grew well, but their rates were slower than that of A, tumefaciens. All strains didn't produce 3-keto-1actose from lactose oxidation. Three strains showed acid reactions and eight strains showed alkaline reactions in litmus milk; but one strain (083) wasn't distinguishable. All fast-growing R. japonicum tested, except strain 066, formed serum ring. Twelve fast-growing R. iaponicum grew in concentrations of up to 0.3-0.4M NaGl, but four of them utilized citrate could tolerate concentrations of 0.45 M NaCl. These isolates formed effective nitrogen-fixing associations with Chinese soybean cuhivars such as Tiefeng 18, Kaiyu 8, 77-153 and so forth. However, dry weight and total nitrogen of above-ground portion were significantly lower than that of slow-growiag R.aponicum. The affinity between fast-growing rhizobia and Kaiyu 8, Tiefeng 18 was significant, but most of them didn't nodulate or formed ineffective nodules with soybean cultivar 77-153.Vigna unguculata and Phaseolus aureus inoculated with fast-growing rhizobia 111, 121,123A individually, except strain 111, other two strains (121, 123A) all produced nodules having activity of nitrogenase. All nodules of plants in pot culture test were identified with corresponding antisera. The result of our experiment showed that nodules of Glycine max, Vigna unguiculata, Phaseolus aureus were formed by fast-growing rhizobia.

从辽宁分离的快生型大豆根瘤菌在柠檬酸盐培养基上表现出两种类型。12株中7株在柠檬酸盐培养基上不生长,5株生长良好。但生长速度比A.tumefaciens慢些。所有菌株都不产生3-酮基乳糖。在石蕊牛奶上除1株以外,都产生血清环,3株产酸,8株产碱1株不明显。在耐盐方面一般能耐0.3 M—0.4MNaCl,而能利用柠檬酸盐的菌株有4株能耐0.45 M Nacl的浓度。这些菌株和我国的栽培大豆品种铁丰18、开育8号、77—153等品种都能进行有效共生。但在干重、植株含氮量上均显著低于慢生型大豆根瘤菌。快型菌株和铁丰18、开育8号亲和性较好、77—153较差。111、121、123 A分别接在豇豆(北京红)和绿豆(D0245—1)、上,除111外其余两株都结瘤并有固氮酶活性。所有结瘤试验都用相应抗血清做了鉴定,证明确系由快生型大豆根瘤菌所结根瘤。另外一些理化特性鉴定正在进行。

Fast-growing R. japonicum QB113 was isolated from nodules of wild soybean(Glycine soja) growing in northeast china. In yeast extract mannitol liquid medium its mean generation time was 3.22 hours and it produced acid. Its physiological-biochemical characteristics were similar to other fast-growing R. japonicum reported. For the first time it was reported that R. japonicum QB113 showed both good infectiveness and effectiveness with common commercial cultivars of soybean in the greenhouse...

Fast-growing R. japonicum QB113 was isolated from nodules of wild soybean(Glycine soja) growing in northeast china. In yeast extract mannitol liquid medium its mean generation time was 3.22 hours and it produced acid. Its physiological-biochemical characteristics were similar to other fast-growing R. japonicum reported. For the first time it was reported that R. japonicum QB113 showed both good infectiveness and effectiveness with common commercial cultivars of soybean in the greenhouse and field experiments. In sterile liquid culture experiment, seed yield of Tiefeng 18 inoculated with QB113 increased 121.7% than that of control, and 55.5% than that of plants inoculated with fast-growing R. japonicum USDA193, and 9.1% than that of plant inoculated with slow-growing R. japonicum 61A76. The same treatments were conducted on Heinong 26, Hefeng 25, 82-7799-2 soybean varieties in field experimental plot, and 20 hectare in the South(planosol area) and North(black soil area)of Wan Da mountain, Heilongjiang Province. The seed yield of plant inoculated with QB113 increased 8.6%, 10.0% and 15.7% respectively than that of uninocu-laed. The results of our experiments showed that the fast-growing R. japonicum QB113 can form effective symbiosis with the soybean culti-var, and proposed to a definite valuable prospects in increasing the yield of soybean production.

从东北地区分离的快生型大豆根瘤菌QB113(fast-growing R.japonicum QB113)在酵母——甘露醇液体培养基上生长时,世代时间为3.22小时,代谢过程产酸,生理生化特性与国内外报导的快生型大豆根瘤菌相类似。我们首次报导了快生型大豆根瘤菌对栽培大豆表现的良好共生固氮能力和增产效果。在实验室的无菌水培实验中,铁丰18大豆接种QB113,种子产量比不接菌的对照提高121.7%,比接种快生型大豆根瘤菌USDA193的提高55.5%,比接种慢生型大豆根瘤菌61A76的提高9.1%,在黑龙江省莞达山南麓(白浆土地区)、北麓(黑土地区)的田间小区试验和大面积(20公顷)试验中,对黑农26、合丰25、82—7799—2大豆进行同样的接菌处理,接种QB113的种子产量比对照分别提高8.6%、10.0%和15.7%。结果说明快生型大豆根瘤菌QB113是可以与栽培大豆品种形成有效共生的。并且,在生产应用中具有一定的价值和可观的前景。

A new group of soybean rhizobia-extra-slow-growing soybean rhiz-obia was obtainted from wild soybean(Glycine soja) nodules in Liaoning province. It nodulated cultivated soybean excellently and could form effective association with mung been(Phaseclus aureus), but not nodulated on pea(Pisum sp), miknetch(letus sp), sweetclover (Melilotus sp.)and astraglus (A. sinicus). The new group did not belong to 135 serogroup. Its ability of alkaline production was especially strong,...

A new group of soybean rhizobia-extra-slow-growing soybean rhiz-obia was obtainted from wild soybean(Glycine soja) nodules in Liaoning province. It nodulated cultivated soybean excellently and could form effective association with mung been(Phaseclus aureus), but not nodulated on pea(Pisum sp), miknetch(letus sp), sweetclover (Melilotus sp.)and astraglus (A. sinicus). The new group did not belong to 135 serogroup. Its ability of alkaline production was especially strong, after cultivation 5 days. PH reched 7.72-8.09. They have an extra-long generation time, for example strain 2064 has a doubling time of approximately 25.9h.

1982年从辽宁省铁岭地区的野大豆根瘤中分离得到一个大豆根瘤菌新类群—超慢型大豆根瘤菌。此类群在栽培大豆上结瘤良好,但在豌豆、百脉根、紫云英、草木樨上不结瘤;在绿豆上能结瘤,不属135血清型。突出的特点是产碱能力强,培养5天后pH达到7.72—8.05,代时超长,被测定的2064菌株代时为25.9小时。

 
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