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high speed centrifugation
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  高速离心
     The average entrapment efficiency of insulin was 95.54+ 1 . 14% (n=3 ) by treatment of high speed centrifugation and filtration.
     高速离心过滤法测得的平均药物包封率为95.54±1.14%(n=3)。
短句来源
     Research result indicates that high speed centrifugation and UF can separate more than 60% protein, making the purity of GABA increase from 3.19% to 6.20%. The recovery of GABA is 88.60%.
     实验结果表明,高速离心和超滤可以截留60%以上的蛋白质,经高速离心和超滤后,GABA的纯度可由最初的3.19%增至6.20%,回收率达88.60%。
短句来源
     Twotypes of IFs-DF_1, DF_2 were obtained through high speed centrifugation at low temperature,Sephadex G-100 gel filtration, and DEAE-cellulose ion-exchange chromatography.
     通过低温高速离心,SephadexG-100凝胶过滤,DEAE离子交换层析等步骤从人精浆中分离出两种免疫抑制因子——DF_1和DF_2。
短句来源
     The protein in serum sample was precipitated by methanol, and the supernatant was directly injected into HPLC after high speed centrifugation.
     血清标本经甲醇沉淀蛋白,高速离心后取上清液直接进样。
短句来源
     Objective:To compare the different influences for preparing technology resulted from respective preparing method of high speed centrifugation and water ethanol method.
     目的:比较高速离心方法与常规水醇方法对制备工艺的影响.
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  高速离心法
     Methods Liposome-entrapped mitoxantrone were prepared by reverse-phase evaporation (REV), high speed centrifugation was applied to separate LEM and dissociative DHAQ, colorimetry was employed to determine encapsulation efficiency, the liposomes’structure and particle size were determine by transmission electron microscopy.
     方法用逆向蒸发法制备LEM,高速离心法分离LEM和游离的DHAQ溶液,比色法测定包封率,透射电镜下观察LEM的结构和粒径,再以戊二醛交联法偶联GM-CSF和LEM制备出LEM-GM-CSF,紫外分光光度法测定偶联率,流式细胞术测定LEM-GM-CSF中GM-CSF的活性保留率。
短句来源
     Method: To prepare Zhike Dingchuan Koufuye with different method of high speed centrifugation and water ethanol method, then measure the respective content obtained by those two different methods.
     方法:采用高速离心法与水醇法同时制备止咳定喘口服液,并测定其含量.
短句来源
     Results: the method of high speed centrifugation could shorten preparing technological process, and much more contents obtained by it than that was obtained by the normal method of water ethanol method.
     结果:高速离心法缩短工艺流程,含量也明显高于常规水醇方法.
短句来源
     Conclusion:the preparing method of high speed centrifugation could replace the normal preparing method of water ethanol method.
     结论:高速离心法可取代常规水醇制备方法.
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     The maintainance of turbine oil is critical for the safely running of the uint. The type and harm of the oil pollution is pressented in the paper,and the oil purification technology is analyzed,such as gravitational separation,filtration,high speed centrifugation and so on.
     汽轮机油的维护对于机组的安全运行至关重要,文章简单介绍了汽轮机油污染的形式及危害,重点分析了国内外常用的油净化技术,如重力分离法、过滤法、高速离心法等。
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  “high speed centrifugation”译为未确定词的双语例句
     The average size and zeta potentials value of MTX CS-NPs prepared under the optimal conditions were 223.5 +121.0nm and 45.2+ 1.6mv respectively. The average entrapment efficiency of MTX was 31.46 + 0.86%(n=3 )by treatmentof high speed centrifugation and filtration.
     以优选的处方与制备工艺制得的MTx壳聚糖纳米粒,其平均粒径为223 .5士121.onm,Zeta电位为45.2士1.6mv,平均药物包封率为31.46士0.86%(n二3)。
短句来源
     Three sample processing methods for mice feces, including low and high speed centrifugation, binding to glass powder, and selective enrichment cultivation, were used for the extraction of DNA template, and the corresponding sensitivities of the methods were 2×10 4,5×10 3,10 CFU, respectively.
     比较了不同方法即差速离心集菌、玻璃粉吸附和选择性增菌处理粪便样品制备模板 DNA,相应于 3种方法的 PCR检测灵敏度分别为 :2× 1 0 4 ,5× 1 0 3,1 0个 CFU。
短句来源
     Objective To prepare liposome-entrapped mitoxantrone (LEM)-GM-CSF and observe the cytotoxicity of HL-60/ADM cells treated with LEM-GM-CSF, LEM and dihydroxyanthraquinone (DHAQ) in vitro. Methods LEM was prepared by reverse phase evaporation (REV). High speed centrifugation was applied to separate LEM and dissociate DHAQ.
     目的制备粒-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)耦联米托蒽醌脂质体(liposome-entrapped mitoxantrone,LEM),比较其与LEM和米托蒽醌(dihydroxyanthraquinone,DHAQ)对HL60/ADM细胞的体外杀伤作用。
短句来源
     1. High speed centrifugation instrument
     1.高速离心机;
短句来源
     Ascites antibody to SPFMV-LF was produced in BALB/c miceimmunized with virus preparation purified from SPFMV-LF infected I. setosaafter twice high speed centrifugation in sucrose cushion.
     病毒颗粒长860—830nm,经两次蔗糖垫超速离心从SPFMV—LF感染的I. setosa叶片提取病毒并在BALB/C小鼠中制备抗SPFMV—LF的腹水多克隆抗体,免疫电镜表明,SPFMV—LF的抗体和SPFMV—RC株系有反应。
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  high speed centrifugation
Serumblocking protein was prepared by treatment of the purified virus with potassium hydroxide followed by either high speed centrifugation or filtration.
      
Their distribution in the fractions isolated by differential high speed centrifugation of homogenates of disintegrated bacterial cells was studied.
      
Soil solutions were obtained by high speed centrifugation and ultrafiltration (0.2 μm) of samples at 60% water-holding capacity.
      
A simple technique for extracting samples of soil solution by high speed centrifugation is described.
      
A simple method for the extraction of soil solution by high speed centrifugation
      
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A convenient method for the preparation of rat liver cell membrane has been described. A fairly pure preparation can be obtained through two runs of low-speed centrifugation and a single run of high-speed centrifugation in sucrose gradient. The preparation has been characterized by structural and biochemical identification by means of electron microscopy, labeled enzyme determination and by insulin-binding experiments.

本文介绍了一个分离大鼠肝细胞膜的简便方法。主要是经过两次低速离心,一次蔗糖梯度超速离心,即可得到一定纯度的细胞膜。对所得细胞膜进行了电镜下的形态学鉴定,标志酶的生化学鉴定及胰岛素结合实验,并对所得结果进行了讨论。

Influenza A viruses were purified by means of adsorption and elution with formalinized chick RBC. After subjected to Sephadex G-150 chromato-graphy, they were further purified by high-speed centrifugation. The purified viruses were cleaved with 1-2% DOC for 30-60 minutes at 37℃and then subjected to another Sephadex G-150 column and eluted with 0.02M Tris-HC1 buffer, pH 8.0. Fractions containing hemagglutinin (HA) activity were pooled and concentrated with PEG until the total volumes were below...

Influenza A viruses were purified by means of adsorption and elution with formalinized chick RBC. After subjected to Sephadex G-150 chromato-graphy, they were further purified by high-speed centrifugation. The purified viruses were cleaved with 1-2% DOC for 30-60 minutes at 37℃and then subjected to another Sephadex G-150 column and eluted with 0.02M Tris-HC1 buffer, pH 8.0. Fractions containing hemagglutinin (HA) activity were pooled and concentrated with PEG until the total volumes were below 20 ml, then passed through a DEAE cellulose column (Cl-type) and eluted with NaCl gradients (0.1-0.8M) . Finally, the purified neuraminidase (NA) and HA were separated into two distinct peaks. Polyacrylamide gel electro-phoresis of the purified HA and NA showed that both preparations developed one single band on the gels.

用甲醛化鸡红细胞吸附释放及葡聚糖G150柱层析,继之以高速离心沉淀制备纯化甲型流感病毒,纯度达3×10~5HAu/毫克蛋白。再经1~2%脱氧胆酸钠于37℃裂解后,用葡聚糖G150及DEAE纤维素(Cl-型)柱层析,经NaCl梯度洗脱,HA及NA分别洗脱于二个洗脱峰。圆盘电泳的结果表明,纯制的HA和NA在聚丙烯酰胺凝胶上于病毒的HA和NA各呈现单一的区带。

This paper reports the radioactive ligand binding technique for GABA receptor and the biochemical char acteristics of GABA receptor of ox cerebellum.The crude synaptic membrane fraction of ox cerebullum was prepared by high speed centrifugation. 3H-GABA binding to receptor on synaptic membrane is a saturate process, and with a dissociation constant of 96 nM, maximum number of binding site of 1.02 Pmol/mg protein and Hill number of 0.99. In binding with GABA receptor, compounds differ from each other in...

This paper reports the radioactive ligand binding technique for GABA receptor and the biochemical char acteristics of GABA receptor of ox cerebellum.The crude synaptic membrane fraction of ox cerebullum was prepared by high speed centrifugation. 3H-GABA binding to receptor on synaptic membrane is a saturate process, and with a dissociation constant of 96 nM, maximum number of binding site of 1.02 Pmol/mg protein and Hill number of 0.99. In binding with GABA receptor, compounds differ from each other in their pharmacological and stereo-scopic specificities. The kinetic constants of GABA receptor binding activity were determined by observed rate method. The rate con-stants for association and dissociation were 9.6×106 M-1 min-1 aud 0.115 min-1 respectively. The activty was increased to 114 fold by supersonic, freezing, thawing, washing and Triton X-100 treatment.

用放射性配基结合实验方法鉴定了牛小脑γ-氨基丁酸(GABA)受体的生化特性。高速离心制备的牛小脑突触膜与~3H-GABA有一饱和结合,K_D值为96nM,B_(max)为1.02pmol/mg蛋白,Hill系数为0.99。这一结合具有药理专一性和立体专一性,动力学实验测得结合速度常数为9.6×10~6M~(-1)min~(-1),解离速度常数为0.115min~(-1)。冻融、洗涤、超声波和TritonX-100处理突触膜,使~3H-GABA结合活性增加114倍。

 
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