助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   expression quantity 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
基础医学
肿瘤学
更多类别查询

图标索引 历史查询
 

expression quantity
相关语句
  表达量
     The relative expression quantity of PTEN in grade Ⅳ was 0^8323, which was significantly lower than that in grade Ⅱ 4^3533 and grade Ⅲ 2^4487 (P<0.01).
     PTEN相对表达量Ⅳ级(0.8323)显著低于Ⅱ(4.3533)、Ⅲ级(2.4487)(P<0.01)。
短句来源
     Results The expressions of TGFβ1 and ALK5 mRNA increased significantly in BAVM,and their relative expression quantity were 0.777±0.047 and 0.585±0.074,respectively.
     结果TGFβ1及其受体ALK5mRNA在脑动静脉畸形中的表达均显著性升高,其相对表达量分别为0.777±0.047和0.585±0.074;
短句来源
     2. The factor Ⅸ expression quantity of G1NaCⅨ in NIH3T3 was 6Ong/106 cells·day-1, 158Ong/106 cells·day-1 in C2 C12 and 3600ng/106 cells·day-1 in HT1O8O of them, 8O % - 9O % of the factor Ⅸ had clotting activity.
     (2)G1NaCⅨ在N1H3T3中的Ⅸ因子表达量为60ng/106细胞·天-1,在C2C12中的Ⅸ因子表达量为1580ng/106细胞·天-1,在HT1080中的Ⅸ因子表达量为3600ng/106细胞·天-1,其中80%~90%的Ⅸ因子具有凝血活性。
短句来源
     Results The expression quantity of MMP-7mRNA in transfected KATOIII gastric carcinoma cell(MMP-7/β-actin O.D.odds is 0.305±0.02) is obviously lower than that of the control group(1.590±0.012), PS-sODN group(1.140±0.03) and PS-mODN group(1.508±0.01)( P<0.05).
     ②MMP -7反义寡核苷酸转染的KATOIII细胞MMP-7mRNA表达量(MMP -7/β-actin光密度比值为0.305±0.02)明显低于对照组、PS -sODN及PS -mODN组(分别为1.590±0.012 ,1.140±0.03 ,1.508±0.017) ,P<0.05。
短句来源
     Mean expression quantity was (67±23)%,(58±19)% and (62±21)% in signet ring cells carcinoma,low-differentiated denocarcinoma and tubular adenocarcinoma,respectively. There was no significant difference between them (P> 0.05).
     印戒细胞癌、低分化腺癌、管状腺癌平均表达量为(67±23)%、(58±19)%、(62±21)%,组间差异无统计学意义(P>0.05)。
短句来源
更多       
  “expression quantity”译为未确定词的双语例句
     It was found that the expression quantity of expression vector pcDNA3.1(+)TPA in human skin fibroblasts transferred group was higher than that in non-transferred group, the protein quantity of transferred group was 647.8 ng/10 6cell·24h as measured by enzyme-linked immunosorbent assay, while that of non-transferred group was 19.2 ng/10 6 cell·24h.
     结果发现 :真核表达载体pcDNA3.1(+)TPA在人皮肤成纤维细胞中获有效表达 ,酶联免疫吸附法TPA蛋白表达定量检测结果为 6 4 7.8ng/(10 6细胞·2 4h) ,未转pcDNA3.1(+)TPA的人皮肤成纤维细胞测得为 19.2ng/ (10 6细胞·2 4h) ;
短句来源
     Objective To detect the expression quantity and To investigate signal transduction of ras-related C3 botulinum toxin substrate,Rac1 and T lymphoma invasion and metastasis inducing factor 1,Tiam 1 in gastric cancer tissues、tissues around the cancer andgastric benign lesion tissues,and To investigate its effect in gastric cancer metastatic.
     目的研究T淋巴瘤侵袭转移诱导因子1(Tlymphoma invasion and metastasis inducingfactor 1,Tiam1)与Ras相关的C3肉毒素底物1(ras-related C3 botulinumtoxin substrate,Rac 1)信号转导及检测其在胃癌组织、癌旁组织、胃良性病变组织中的量值水平,进一步探讨其在胃癌转移中的作用。
短句来源
     Western blotting revealed 14-3-3 protein showed a basic expression in Contral group. The expression quantity of 14-3-3 protein in PD98059+Control group was similar to that of Control and 0.98±0.12 fold of Control group(p>0.05).
     14-3-3蛋白的表达:Control组心肌细胞有少量14-3-3蛋白表达,PD98059+Control组为Control组的0.98±0.12倍,两组之间无显著性差异(p>0.05);
短句来源
     (3) The expression quantity of PTEN gene in the low-differentiated HCC tissue was also much lower than that in the high and middle differentiated HCC tissues(P<0.01);
     该基因在癌旁组织和正常组织中的表达水平无显著差异(P>0.05); ③低分化肝癌组织中PTEN基因表达水平明显低于高、中分化肝癌组织中PTEN基因表达水平(P<0.01);
短句来源
     The result indicate that H5HA and H7HA can all be expressed in mammalian cell, in addition, the expression quantity of H5HA protein is higher than H7HA.
     结果表明双顺反子真核表达载体构建正确 ,且在真核表达细胞系中 H5HA和 H7HA基因都能获得表达 ,且 H5HA 基因的表达效果优于H7HA。
短句来源
更多       
  相似匹配句对
     On the Expression of Approximate Quantity in Chinese
     汉语“约量”的表达研究
短句来源
     On Quantity
     论量
短句来源
     The Cognitive and Semantic Motivation of Object Quantity Expression
     物体量表达的认知语义基础
短句来源
     On "Expression of the Abstract
     论“抽象的抒情”
短句来源
     On Chinglish expression
     论中式英语的表现形式
短句来源
查询“expression quantity”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  expression quantity
The analysis of in silico expression profile showed that ESTsp3 is expressed in various growth stages and in most tissues and organs, such as soft tissue, skin, skeletal muscle and kidney, but with variant expression quantity.
      


A 0. 3 kb of hIL- 13 fragment, directly amplified from activated healthy human peripheral blood mononuclear cells (HPBMC) by RT-PCR method, was separately inserted into high expression vectors pBV220 and pGEX-4T-2 containing tac promotor, lac I repressor and thrombin recognition site so as to construct prokaryotic non-fusion-protein expression bacterial IL- 13-pBV220/DH5a and fusion protein expression bacterial IL- 13-pGEX-4T-2/TG1. After heat induction (42℃ ) for the former engi neering bacterial...

A 0. 3 kb of hIL- 13 fragment, directly amplified from activated healthy human peripheral blood mononuclear cells (HPBMC) by RT-PCR method, was separately inserted into high expression vectors pBV220 and pGEX-4T-2 containing tac promotor, lac I repressor and thrombin recognition site so as to construct prokaryotic non-fusion-protein expression bacterial IL- 13-pBV220/DH5a and fusion protein expression bacterial IL- 13-pGEX-4T-2/TG1. After heat induction (42℃ ) for the former engi neering bacterial and chemical induction (IPTG) for the latter, IL- 13 got expressed in E.coli only in an GST fusion-protein form, and the GST-IL-13 expression quantities took up about 10% ~ 30% of the total bacterial proteins. The expression of IL- 13 monomers in prokaryocytic cells was quite low-lev el.

采用RT-PCR技术直接从激活的健康人外周血单个核细胞中扩增得到hIL-13的基因片段,通过DNA重组技术分别将其插入到含有PRPL启动子的高效表达载体pBV220及含tac启动子、lac1阻遏蛋白基因及凝血酶识别位点的融合蛋白表达载体pGEX-4T-2中,成功地构建了hIL-13的原核非融含蛋白表达菌株hIL-13-PBV220/DHS5a及融合蛋白表达菌株hIL-13-PGEX-4T-2/TG1,分别经42℃热诱导及异丙基硫代半乳糖苷(IPTG)化学诱导后,SDS-PAGE结果表明IL-13仅以GST融合蛋白的形式在E.coli中获得了表达,表达量约占菌体蛋白总量的10%~30%。IL-13的蛋白单体在原核细胞中表达量很低。

Objective To construct a highly effective and safe retrovirus vector G1NaCIX that can be used for gene therapy. Meth0ds The CW - FⅨ cDNA fragment was cut down from plasmid pCMVⅨ -1O by restriction endonuclease Bgl Ⅱand Hind Ⅲand linked with G1Na which had been reacted with BamHI and Hind Ⅲ. The new vector proved to be linked correctly was named G1NaCⅨ and was transduced by packing cell PA317 into cell lines fibroblasts NIH3T3, myoblasts QC12 of mice and human fibrosarcoma cells HT1O8O. After selecting culture...

Objective To construct a highly effective and safe retrovirus vector G1NaCIX that can be used for gene therapy. Meth0ds The CW - FⅨ cDNA fragment was cut down from plasmid pCMVⅨ -1O by restriction endonuclease Bgl Ⅱand Hind Ⅲand linked with G1Na which had been reacted with BamHI and Hind Ⅲ. The new vector proved to be linked correctly was named G1NaCⅨ and was transduced by packing cell PA317 into cell lines fibroblasts NIH3T3, myoblasts QC12 of mice and human fibrosarcoma cells HT1O8O. After selecting culture for two weeks, the factor Ⅸexpression quantity of G1NaCⅨ in transfected cells was determined. Results 1. It was testified by enzyme sever electrophoresis that G1NaCⅨ had been linked correctly. 2. The factor Ⅸ expression quantity of G1NaCⅨ in NIH3T3 was 6Ong/106 cells·day-1, 158Ong/106 cells·day-1 in C2 C12 and 3600ng/106 cells·day-1 in HT1O8O of them, 8O % - 9O % of the factor Ⅸ had clotting activity. Conclusion Successfully constructed retrovirus vector G1NaC Ⅸ can express highly efficiently in cell C2C12 and cell HT108O.

目的构建高效、安全、可用于基因治疗的反转录病毒载体G1NaCⅨ。方法用限制性内切酶Bg1Ⅱ和HindⅢ将CMV-FⅨcDNA从质粒pCMVⅨ-10上切下,将该片段与BamHⅠ和HindⅢ双酶切后的G1Na连接,连接后的载体经鉴定连接正确后即为新载体G1NacⅨ,将G1NaCⅨ通过包装细胞PA317导入小鼠成纤维细胞NIH3T3、小鼠成肌细胞C2C12和人纤维肉瘤细胞HT1080中,选择培养两周后分别测定G1NaCⅨ在上述细胞中的Ⅸ因子表达量。结果(1)经酶切电泳鉴定,G1NaCⅨ连接正确;(2)G1NaCⅨ在N1H3T3中的Ⅸ因子表达量为60ng/106细胞·天-1,在C2C12中的Ⅸ因子表达量为1580ng/106细胞·天-1,在HT1080中的Ⅸ因子表达量为3600ng/106细胞·天-1,其中80%~90%的Ⅸ因子具有凝血活性。结论构建成功的反转录病毒载体G1NaCⅨ在C2C12和HT1080细胞中均能高效表达。

Aim:To acquire a large amount of anti human TNF alpha single chain antibody,we investigated the effect of induction condition on quantity of expression products. Methods: The E6ScFv gene fragment was cloned into pET15b Etag and pBV220 expression vectors,respectively for constructing the recombinant expression plasmids pETE6ScFv and pBVE6ScFv. After inducing with IPTG or at 42℃, the quantities of the two expression products at same times were identified by scanning bands...

Aim:To acquire a large amount of anti human TNF alpha single chain antibody,we investigated the effect of induction condition on quantity of expression products. Methods: The E6ScFv gene fragment was cloned into pET15b Etag and pBV220 expression vectors,respectively for constructing the recombinant expression plasmids pETE6ScFv and pBVE6ScFv. After inducing with IPTG or at 42℃, the quantities of the two expression products at same times were identified by scanning bands on their SDS PAGE gel with CS 9000 thin scanner. Results: After inducing with IPTG for 2 hours, a new protein band of E6ScFv Etag fusion protein in the pETE6ScFv(BL21)was found on SDS PAGE gel. And a new protein band in the pBVE6ScFv(DH5α)was found after inducing for 4 hours at 42℃. Their quantities were increased with the induction time prolonged. On the contrary,expression quantities of both recombinants were decreased by 20 hours'induction. Conclusion: The optimal period of induced with IPTG for pETE6ScFv(BL21)was 4~6 hours, while that at 42℃for pBVE6ScFv(DH5α)was 8 hours.

目的: 探讨诱导时间对目的蛋白表达量的影响, 为大量获得抗TNFα单链抗体提供实验依据。方法:将E6ScFv 基因分别克隆入表达载体pET15bEtag 和pBV220 中, 构建重组表达质粒pETE6ScFv 和pBVE6ScFv。在化学诱导和温度诱导两种条件下, 于诱导后相同时间点等量取菌体, 用SDSPAGE 对各时间点表达产物扫描定量。结果: pETE6ScFv (BL21) 的阳性克隆过夜菌经IPTG 化学诱导2 h, 就有新生蛋白条带出现; 而pBVE6ScFv (DH5α) 诱导4 h 后才有新生蛋白条带出现。两者均随诱导时间的延长而加深, 但将诱导时间延长至20 h 表达量并无明显变化, 表达水平反而下降。结论: pETE6ScFv (BL21) 的最佳诱导时间为4 h ~6 h,pBVE6ScFv (DH5α) 的最佳诱导时间为8 h 。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关expression quantity的内容
在知识搜索中查有关expression quantity的内容
在数字搜索中查有关expression quantity的内容
在概念知识元中查有关expression quantity的内容
在学术趋势中查有关expression quantity的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社