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   bacterial expression 的翻译结果: 查询用时:0.204秒
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bacterial expression
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  原核表达
     The results showed that HPV16L1 protein formed inclusion bodies in bacterial expression system,suggesting that this assay can be used to purify HPV16L1 protein and hence provide a basis for studying the applications of HPV16 L1 protein.
     结果显示 ,HPV16 L1蛋白在原核表达系统以不溶性包涵体形式存在 ,通过本方法可以获得纯化的 HPV16 L1蛋白 ,为 HPV16 L1的应用研究打下了基础
短句来源
     Results HPV16L1 proteins formed inclusion bodies in bacterial expression system, this assay could purified HPV16L1 proteins.
     结果 HPV16L1蛋白在原核表达系统以不溶性包涵体形式存在 ,通过本方法可以获得纯化的蛋白。
短句来源
     Methods By adapting reverse transcription PCR technology, we obtained the cDNA clones encoding β2GP1, β2GP1 D5, U1RNP70KD, U1BD, Sm D and SSB autoantigens. The recombinant proteins were expressed by the bacterial expression plasmid pGEX 2T and then idnetified and purified. The locations of epitopes of β2GP1 and U1RNP70KD were analysed by competitive inhibition ELISA and IBT respectively.
     方法 应用逆转录PCR方法克隆 β2GP1及其第 5功能区 (D5 ) ,U1RNP70KD及其U1RNA结合功能区 (BD) ,Sm D和SSB的编码基因 ,经原核表达系统 (PGEX 2T)进行表达、鉴定及纯化 ,并分析 β2GP1及U1RNP70KD抗原表位的定位 ,进而以重组抗原为基质建立新型免疫印迹及酶联免疫吸附检测法。
短句来源
     In order to get the antiserum of PVY-C Ma, bacterial expression vectors containing PVY-C 6kD-Nla and VPg gene were constructed, respectively.
     为了制备PVY-C NIa的抗血清,首先构建了6kD-NIa和VPg基因片段的原核表达载体。
短句来源
     By adapting reverse transcription-PCR technology,we obtained the cDNA cloneencoding U1RNA binding domain(U1BD)of the autoantigen U1SnRNP 70kD polypeptlde,using HeLa cell total RNA as template. Confirmed by DNA sequencing,the target U1BD cDNA was subcloned unindirectionally into the bacterial expression plasmid PGEX-2T and then transformed into E.
     本研究采用逆转录-PCR技术克隆自身核抗原U1SnRNP70kD多肽分子中U1RNA结合功能区(U1RNAbindingdomain,U1BD)的cDNA,经DNA测序证实以后定向插入原核表达载体PGEX-2T,进而导入大肠杆菌中表达重组蛋白。
短句来源
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  细菌表达
     tox 34 was inserted into bacterial expression vector pBV221. Electrophoresis of protein mixture extracted for the bacteria treated with heat-induction showed that specific protein bands migrated with apparent molecular weight of 32 ~ 33 kD in denaturing gels.
     将改造后的基因插入细菌表达载体pBV221,经热诱导处理后细菌总蛋白在SDS-PAGE上特异条带的表观分子量为32~33kD。
短句来源
     To obtain abundant βB2 crystallin for the study of the mechanism of their oligomerization, a bacterial expression system for βB2 crystallin and a rapid method for its purification were developed.
     为了获得大量纯的βB2晶体蛋白并用定点突变的方法研究其聚合的机制, 建立了大鼠βB2晶体蛋白的细菌表达系统及纯化方法。
短句来源
     Results The sequence of the cloned human open reading frame of hIL18 was exa ctly the same as that reported in GenBank. (Accession Number:E17135). SDSPAGE di splayed that the size of the bacterial expression product was approximately 18.3 KDa.
     结果 所克隆的人IL 1 8cDNA编码序列经测序证实与GenBank所报道的序列完全一致 ,细菌表达产物经SDS PAGE证实其大小约为 1 8.3KDa,WesternBlot进一步证实了所表达产物的正确性。
短句来源
     [Objective] HCCR1 and HCCR2 are the oncogenes overexpressed in hepatocellular carcinomas(HCC). In this experiment,it was evaluated that the cloning,bacterial expression,purification and characterization of soluble recombinant proteins of HCCR1 and HCCR2 for novel early diagnosis of HCC.
     目的评估HCCR1和HCRR2克隆、细菌表达及可溶性蛋白的纯化及应用于早期肝癌诊断的可行性。
短句来源
  细菌表达
     tox 34 was inserted into bacterial expression vector pBV221. Electrophoresis of protein mixture extracted for the bacteria treated with heat-induction showed that specific protein bands migrated with apparent molecular weight of 32 ~ 33 kD in denaturing gels.
     将改造后的基因插入细菌表达载体pBV221,经热诱导处理后细菌总蛋白在SDS-PAGE上特异条带的表观分子量为32~33kD。
短句来源
     To obtain abundant βB2 crystallin for the study of the mechanism of their oligomerization, a bacterial expression system for βB2 crystallin and a rapid method for its purification were developed.
     为了获得大量纯的βB2晶体蛋白并用定点突变的方法研究其聚合的机制, 建立了大鼠βB2晶体蛋白的细菌表达系统及纯化方法。
短句来源
     Results The sequence of the cloned human open reading frame of hIL18 was exa ctly the same as that reported in GenBank. (Accession Number:E17135). SDSPAGE di splayed that the size of the bacterial expression product was approximately 18.3 KDa.
     结果 所克隆的人IL 1 8cDNA编码序列经测序证实与GenBank所报道的序列完全一致 ,细菌表达产物经SDS PAGE证实其大小约为 1 8.3KDa,WesternBlot进一步证实了所表达产物的正确性。
短句来源
     [Objective] HCCR1 and HCCR2 are the oncogenes overexpressed in hepatocellular carcinomas(HCC). In this experiment,it was evaluated that the cloning,bacterial expression,purification and characterization of soluble recombinant proteins of HCCR1 and HCCR2 for novel early diagnosis of HCC.
     目的评估HCCR1和HCRR2克隆、细菌表达及可溶性蛋白的纯化及应用于早期肝癌诊断的可行性。
短句来源
  “bacterial expression”译为未确定词的双语例句
     Methods A cDNA fragment encoding for rat AMP-activated protein kinase α2(AMPKα2) was amplified by PCR and inserted into bacterial expression vector pBT.
     方法PCR扩增大鼠AMP激活的蛋白激酶α2(AMP-ac-tivated prote in k inaseα2,AMPKα2)蛋白编码区cDNA,与大肠杆菌双杂交表达载体pBT构建融合蛋白表达质粒pBT-AMPKα2。
短句来源
     Methods: Using DNA recombinant techniques, the 6B11scFv/hGM CSF fusion genes were subcloned into bacterial expression vector pET16(a+) to produce soluble proteins.
     方法:用DNA重组技术,将以前构建的6B11scFvLinkerhGMCSF融合基因克隆至pET16(a+)表达载体,表达可溶蛋白质。
短句来源
     A cDNA encoding acidic phospholipase A 2I(A.aAPLA 2I)from Agkistrodon acutus was inserted into a bacterial expression vector and effectively expressed in E.
     将尖吻蝮蛇毒酸性磷脂酶 A2 I( A.a A P L A2 I) 的基因克隆至表达载体p B L M V L2 , 在大肠杆菌 R R1 中成功表达。
短句来源
     Methods IL-10 cDNA was subcloned into the PCRT7 /NT-TOPO bacterial expression vector.
     方法 用构建成功的IL-10-PCR(?) T7/NT-TOPO(?)
短句来源
     In present study, a recombinant expression plasmid SYNS1/pET28a was constructed by cloning the coding sequence for NS1 into pET28a, a bacterial expression vector.
     将SYNS1/pET2 8a转化到大肠杆菌BL2 1(DE3)中 ,经IPTG诱导 ,使PPVNS1基因获得了表达 ,运用ELISA和Westernblotting证实了表达产物的特异性。
短句来源
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  bacterial expression
Aim of study: To investigate if an association exists between in-vivo mucosal levels of IL-8 and bacterial expression of cytotoxin and cagA gene of H.
      
Monospecific antiserum prepared to the 5-AR bacterial expression product specifically immunoprecipitated a protein of approximately 11.6 kDa from ASFV infected swine macrophages at late times post-infection.
      
The cDNA encoding the VP6 gene of avian rotavirus PO-13 strain was inserted into the bacterial expression vector pET-3a.
      
Furthermore, cDNA of S9 from each isolate incorporated into the bacterial expression vector pGEX3-X produced a fusion protein that reacted with antibodies raised against purified RRSV particles.
      
The DNA containing the putative CP was cloned into the pUEX 2 bacterial expression vector and expressed inEscherichia coli as a β-gal-CP fusion protein.
      
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By adapting reverse transcription-PCR technology,we obtained the cDNA cloneencoding U1RNA binding domain(U1BD)of the autoantigen U1SnRNP 70kD polypeptlde,using HeLa cell total RNA as template.Confirmed by DNA sequencing,the target U1BD cDNA was subcloned unindirectionally into the bacterial expression plasmid PGEX-2T and then transformed into E.coli to express the recombinant protein.Further analysis by immunoblotting demonstrated that 96%(48/50)of antiU1SnRNP 70kD positive sera can recognize the recombinant...

By adapting reverse transcription-PCR technology,we obtained the cDNA cloneencoding U1RNA binding domain(U1BD)of the autoantigen U1SnRNP 70kD polypeptlde,using HeLa cell total RNA as template.Confirmed by DNA sequencing,the target U1BD cDNA was subcloned unindirectionally into the bacterial expression plasmid PGEX-2T and then transformed into E.coli to express the recombinant protein.Further analysis by immunoblotting demonstrated that 96%(48/50)of antiU1SnRNP 70kD positive sera can recognize the recombinant protein.Thus,it was shown that the recombinant protein of U1BD exhibited the antigenicity of U1SnRNP70kD, and it may be a major epitope region that can be recognized by a majority of anti-U1SnRNP 70kD antibodies.

本研究采用逆转录-PCR技术克隆自身核抗原U1SnRNP70kD多肽分子中U1RNA结合功能区(U1RNAbindingdomain,U1BD)的cDNA,经DNA测序证实以后定向插入原核表达载体PGEX-2T,进而导入大肠杆菌中表达重组蛋白。免疫印迹法研究表明:96%(48/50)的抗U1SnRNP70kD抗体阳性血清能够识别该重组蛋白,证实U1BD重组蛋白具有U1SnRNP70kD抗原性,而且U1BD是U1SnRNP70kD多肽上主要抗原表位区域,能够被大多数抗U1SnRNP70kD抗体阳性血清识别。这为今后对U1BD抗原表位精确定位,分析特定抗原表位与疾病的相关性以及制备重组抗原用于临床检测等研究奠定基础。

The rat IsL─I cDNA─coding sequence of 543 hp was synthesized by PCR. The cDNA was cloned into bacterial expression vector PQE─32. M15 trasformants containing the recombinant PQE─32/IsL─1 were cultured. After induction with IPTG, the IsL─I fusion protein was obtained. This protein was used to immune rabbits to produce antiserum. Immunocytochemical analysis showed that IsL─Iimmunopositive cells widely exist in the rat CNS. The function of IsL─I in the CNS remains to be investigated.

用PCR方法合成了IsL-1cDNA的一段543bp的编码序列,将该cDNA克隆于表达载体pQE-32中,含pQE-32/IsL-1重组子的M15转化菌株经诱导培养后获得了IsL-1融合蛋白,用该蛋白免疫家兔,产生抗IsL-1抗体,经免疫组化初步检测发现大鼠中枢神经系统内广泛存在IsL-1免疫阳性细胞。IsL-1在中枢神经系统的作用有待研究。

Ohjective:In order to reduce the immunogenicity. we cloned and expressed 3D5ovarian car-cinoma Ab2singlechain Fv(SeFv)genes in E.coli. Methods: Using RT-PCR. the variable regiongeneS of heavyand light chains(VH and VL)of Ab2s hnve heen obtained from3D5 hybridomas. The VHand VL hxve been asxembled with a flexible linker.sequence to encode ScFv sequencesThe latter hasbeen cloned into bacterial expression vectors to produce protein. kesults . Expressed scFv proteins from44%of the checked clones were shown...

Ohjective:In order to reduce the immunogenicity. we cloned and expressed 3D5ovarian car-cinoma Ab2singlechain Fv(SeFv)genes in E.coli. Methods: Using RT-PCR. the variable regiongeneS of heavyand light chains(VH and VL)of Ab2s hnve heen obtained from3D5 hybridomas. The VHand VL hxve been asxembled with a flexible linker.sequence to encode ScFv sequencesThe latter hasbeen cloned into bacterial expression vectors to produce protein. kesults . Expressed scFv proteins from44%of the checked clones were shown to react with the primaryantibody(Ab1).TheDNAfragmentswerefound to be thesamesize as that of the posltive control when theseelonedDNAwere digested withrestriction enzymes or amplified by PCR. Conclusion. The success of tHis study may not only pltqy a rolefor genetically manipulating the Ah2 but alsogive a clue for an active immunotherapy of ovariancancer.

目的:降低3D_5抗独特型抗体鼠源性,促进临床应用。方法:用PCR及基因重组技术,扩增并构建单链可变区抗体基因,与表达载体连接后转化大肠杆菌。结果:ELISA检测37个克隆,其中17个表达产物与相应抗体呈特异免疫反应;重组体限制性内切酶酶切分析及PCR扩增均见特异DNA条带。结论:克隆表达了3D_5抗独特型单链抗体基因,为进一步人源化改造奠定了基础。

 
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