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cofactor regeneration
相关语句
  辅酶再生
     Research Progress in Cofactor Regeneration Systems
     辅酶再生体系的研究进展
短句来源
  辅因子再生
     Progresses on cofactor regeneration
     辅因子再生研究进展
短句来源
  “cofactor regeneration”译为未确定词的双语例句
     coli M15 (pQE30-gdh223) were recombinant strains harboring the ALR gene from Sporobolomyces salmonicolor ZJU0307 and the gdh223 gene from Bacillus megaterium. The cells of these two strains were mixed to construct a reaction system with cofactor regeneration. To reduce the inhibition of the substrate and the product, the reaction was carried in a water/n-butyl acetate two-phase system.
     结果表明,在水/乙酸丁酯的体系中,32℃时在pH 6.0的缓冲溶液中,100g/L的重组菌(M15(pQE30-ALR)/M15(pQE30-gdh223)=4/1)在滴加底物条件下将360mM的COBE还原为(R)-CHBE,产物得率为78.5%,e.
短句来源
     Due to the high cost of the cofactors,in situ cofactor regeneration is required for preparative applications.
     因为辅因子价格昂贵,所以,在酶催化工业应用中,需要实现辅因子原位再生。
短句来源
  相似匹配句对
     Progresses on cofactor regeneration
     辅因子再生研究进展
短句来源
     Research Progress in Cofactor Regeneration Systems
     辅酶再生体系的研究进展
短句来源
     Plant regeneration from I.
     首次从I.
短句来源
     Liver Regeneration
     肝再生
短句来源
     Fe.Mo-Cofactor
     铁钼辅因子
短句来源
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  cofactor regeneration
Chemoenzymatic galactosialylation with integrated cofactor regeneration
      
The results suggest that vapour-solid methodology can be used with whole yeast cells to generate volatile products that require multi-enzymic bioconversions, as well as cofactor regeneration or conversion of ADP to ATP.
      
A mathematical model for the acetophenone reduction with cofactor regeneration describing the behaviour in a batch, repetitive-batch and continuously stirred tank reactor was developed.
      
To achieve 98% conversion of acetophenone, cofactor regeneration by oxidation of 2-propanol with the same enzyme was used.
      
Coenzyme recycling and electrochemical redox cycling as methods for cofactor regeneration are described and commercial applications indicated.
      
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Cofactor regeneration system is the key step for the biocatalysis and biotransformation of chemicals by oxidoreductases. Among the methods developed for the cofactor regeneration, two of the most important methods, enzymatic regeneration and electrochemical regeneration, were reviewed in this paper. In the enzymatic regeneration of NAD(P)H or NAD(P), development of an efficient enzymatic system is the focus of researches. For use of free enzymes in the cofactor regeneration, finding...

Cofactor regeneration system is the key step for the biocatalysis and biotransformation of chemicals by oxidoreductases. Among the methods developed for the cofactor regeneration, two of the most important methods, enzymatic regeneration and electrochemical regeneration, were reviewed in this paper. In the enzymatic regeneration of NAD(P)H or NAD(P), development of an efficient enzymatic system is the focus of researches. For use of free enzymes in the cofactor regeneration, finding new enzymes and the functionally coupling of the enzymatic reactions are important. While for using the whole cells in the enzymatic regeneration system, reaction engineering such as the use of membrane reactors is also indispensable. Efficiency of the electrochemical regeneration of the cofactors is closely dependent upon the electrode modification and selection of the electron mediators. Moreover, perspectives of new regenerating methods such as fusion proteins with multi-functionality, “cofactor engineering" by the metabolic manipulation of whole cells, and multi-species microbial coupling system were also discussed. With the progress of microbial genomics, proteomics, metabolic engineering and systems biology, the cofactor regeneration will achieve breakthroughs and become a feasible tool for the industrial application.

辅酶再生是实现氧化还原酶催化反应的必需步骤 ,是关系到氧化还原酶工业应用的关键。通过介绍酶法再生和电化学方法再生的机理 ,分别总结了有关还原态辅酶和氧化态辅酶再生方法的研究现状 ,包括酶学再生过程中涉及到的酶、自由酶和整体细胞再生及其反应器的设计 ;以及电化学再生中电极修饰及中间体选择等。并提出了辅酶再生研究的潜在发展方向

This article describes oxidising enzymes used for biocatalytic applications.Redox biocatalysts are highly sought after because of the selectivity,controllability and economy of their reactions,in comparison with conventional chemical reactions.Increasing numbers of oxidative biotransformations are being reported,indicating wide variability in the biocatalyst characteristics and a range of potential and established applications.Several limitations apply to oxidative biotransformations,including the requirement...

This article describes oxidising enzymes used for biocatalytic applications.Redox biocatalysts are highly sought after because of the selectivity,controllability and economy of their reactions,in comparison with conventional chemical reactions.Increasing numbers of oxidative biotransformations are being reported,indicating wide variability in the biocatalyst characteristics and a range of potential and established applications.Several limitations apply to oxidative biotransformations,including the requirement for cofactor regeneration,and low stability and activities.Recent advances in addressing these problems include molecular and reaction engineering approaches.

本文论述了蛋白酶及其与生物催化剂的关系,重点论述了生物催化剂氧化酶的分类特征、应用状况和使用过程中的优点与局限性及当前的研究进展。随着分子生物技术和化学工程方法的发展,展望了生物催化剂氧化酶的广阔应用前景。

Cofactor-dependent enzymes catalyze many useful reactions.Due to the high cost of the cofactors,in situ cofactor regeneration is required for preparative applications. After two decades of research,various cofactors ,such as NAD(P)H,ATP,sugar nucleotides can now be regenerated using enzymatic,chemical, electrochemical, photochemical or genetic engineering based methods. The recent advances in cofactors regeneration are reviewed in this paper.

许多有价值的酶催化反应都需要辅因子的参与。因为辅因子价格昂贵,所以,在酶催化工业应用中,需要实现辅因子原位再生。经过几十年研究,出现了酶法、化学法、电化学法、光化学法和基因工程法等手段实现烟酰胺类辅因子(NAD(P)H)、ATP、糖核苷酸等辅因子再生。对辅因子再生研究中取得的进展以及存在的问题进行讨论。

 
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