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heterotopic osteogenesis
相关语句
  异位成骨
    Ability of heterotopic osteogenesis of composites of bone marrow stromal cells transfected with hBMP-2 gene and bioactive glass ceramics
    转染hBMP-2基因的骨髓基质细胞与生物活性陶瓷复合材料异位成骨能力观察
短句来源
    The MSCs and BGC transfected with hBMP 2 gene were of good biocompatibility and ability of heterotopic osteogenesis.
    携带hBMP-2基因的MSC与BGC具有良好的生物相容性,且具有异位成骨能力。
短句来源
    CONCLUSION:MSCs can be used as the recipient cells of hBMP 2 gene for expressing and secreting the hBMP 2 protein, and the composites of the tansfected cells and BGC were proved to have the ability of heterotopic osteogenesis, indicating that transfected MSCs by hBMP 2 protein can be served as seed cells for bone tissue engineering.
    结论:MSC可以作为hBMP-2基因的受体细胞,基因表达可分泌hBMP-2蛋白质,与BGC复合后具有异位成骨能力,认为转染了hBMP-2基因的MSC可作为骨组织工程学中的种子细胞。
短句来源
    AIM:To investigate the ability of heterotopic osteogenesis of the composites of rabbits' bone marrow stromal cells(MSCs) transfected with hBMP 2 gene and bioactive glass ceramics(BGC) after transplanted into the muscle strand of rabbit's thigh.
    目的:观察转染了hBMP-2基因的兔骨髓基质细胞(bonemarrowstromalcells,MSC)与生物活性陶瓷(bioactiveglassceramicsics,BGC)复合后植入兔股部肌袋中生物复合材料的异位成骨能力。 方法:实验时间为2003-07/2004-05。
短句来源
  异位骨形成
    Objective To investigate the ability of heterotopic osteogenesis and reconstruction of critical size calvarial defect, a bone tissue engineering Sprague Dawley rat model with autogenous osteoblasts loading on porous calcium phosphate ceramic was established.
    目的 构建封闭群动物SD大鼠骨组织工程模型 ,研究含孔磷酸钙陶瓷为支架复合自体成骨细胞的异位骨形成和极量骨缺损修复能力。
短句来源
    Results Morphological and histological study demonstrated that cell ceramic compound had the ability of heterotopic osteogenesis.
    结果 细胞 陶瓷复合体无论种植于皮下或肌肉内均表现明显的异位骨形成能力 ;
短句来源
  异位成骨的
    Experimental Research of Heterotopic Osteogenesis of Cancellous Granule-type Calcium Phosphate Cement Blended with Autologus Bone Marrow
    自体骨髓植入对实验性松质骨粒型自固化磷酸钙异位成骨的影响
短句来源
  “heterotopic osteogenesis”译为未确定词的双语例句
    Heterotopic Osteogenesis of Autogenous Marrow Stromal Cells with rhBMP2 Gene Transfection and Porous Calcium Phosphate Ceramic as a Scaffold
    重组人骨形成蛋白2基因转染大鼠自体骨髓基质细胞成骨能力研究
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  heterotopic osteogenesis
Histological and ultrastructural analysis of heterotopic osteogenesis in porous calcium phosphate ceramics
      
Failure of heterotopic osteogenesis by epithelial mesenchymal cell interactions in xenogeneic transplants in the kidney
      
planoepitheliale, n=2) were grafted intramuscularly into cortisone-immunosuppressed mice to test the ability of transformed transitional epithelium to induce heterotopic osteogenesis.
      
The occurrence of different nerve types was analyzed at different stages of heterotopic osteogenesis, induced by allogeneic bone matrix.
      


Objective To investigate the ability of heterotopic osteogenesis and reconstruction of critical size calvarial defect, a bone tissue engineering Sprague Dawley rat model with autogenous osteoblasts loading on porous calcium phosphate ceramic was established. Methods Bone marrow was obtained from femurs and tibias of 20 male adult SD rats under general anesthesia and sterile condition. Autogenous osteoblasts proliferated and differentiated in α minimal essential medium with some special additives for...

Objective To investigate the ability of heterotopic osteogenesis and reconstruction of critical size calvarial defect, a bone tissue engineering Sprague Dawley rat model with autogenous osteoblasts loading on porous calcium phosphate ceramic was established. Methods Bone marrow was obtained from femurs and tibias of 20 male adult SD rats under general anesthesia and sterile condition. Autogenous osteoblasts proliferated and differentiated in α minimal essential medium with some special additives for 14 days. Culture cells were then seeded and subcultured for another 15 days in porous calcium phosphate bioceramic, which had been soaked in fibronectin for surface modification. The cell ceramic compound was implanted subcutaneously and intramuscularly as well as used to repair critical sized (8 mm diameter) calvarial defect in the same rat. Lab animals were sacrificed at two week intervals till 20th week postoperatively and all the samples were dissected.Results Morphological and histological study demonstrated that cell ceramic compound had the ability of heterotopic osteogenesis. It was also observed that reconstruction of critical size calvarial defect with this hybrid material was better than that with solitary ceramic.Conclusion It seems that the experimental results got from this closed colony animal model were more similar to clinical situation. This model is superior to other homogenic or immune deficiency ones.

目的 构建封闭群动物SD大鼠骨组织工程模型 ,研究含孔磷酸钙陶瓷为支架复合自体成骨细胞的异位骨形成和极量骨缺损修复能力。方法 成年雄性SD大鼠 2 0只 ,全麻及无菌条件下取其左侧股骨及胫骨 ,将骨髓冲入 75ml培养瓶中并在诱导培养条件下进行体外扩增分化 ;培养 1 4d后将已具成骨细胞表型的细胞消化接种于经纤连蛋白表面修饰的含孔磷酸钙陶瓷上继续培养 1 5d ,再对应地将细胞 陶瓷复合体种植于大鼠皮下、肌肉内并用于颅骨极量缺损修复。分别于 2~ 2 0周处死动物 ,从形态学及组织学角度观察比较异位骨形成及骨缺损修复情况。结果 细胞 陶瓷复合体无论种植于皮下或肌肉内均表现明显的异位骨形成能力 ;颅骨极量缺损修复也优于同期仅植入经表面修饰但无细胞的陶瓷对照组。结论 应用封闭群动物SD大鼠构建的骨组织工程模型避免了采用免疫缺陷动物及同源近交系动物价格昂贵、近交衰退等缺点 ,其结果接近于临床 ,因而更具有说服力

AIM:To investigate the ability of heterotopic osteogenesis of the composites of rabbits' bone marrow stromal cells(MSCs) transfected with hBMP 2 gene and bioactive glass ceramics(BGC) after transplanted into the muscle strand of rabbit's thigh. METHODS:The experiment was conducted in the Institute of Bone Tissue Engineering for Anatomy,Jinzhou Medical College from July 2003 to May 2004.With the help of lipofectamine, the cultured MSCs were transfected with the recombinant carrier PcDNA3 hBMP2 carrying...

AIM:To investigate the ability of heterotopic osteogenesis of the composites of rabbits' bone marrow stromal cells(MSCs) transfected with hBMP 2 gene and bioactive glass ceramics(BGC) after transplanted into the muscle strand of rabbit's thigh. METHODS:The experiment was conducted in the Institute of Bone Tissue Engineering for Anatomy,Jinzhou Medical College from July 2003 to May 2004.With the help of lipofectamine, the cultured MSCs were transfected with the recombinant carrier PcDNA3 hBMP2 carrying hBMP 2 gene and the positive cells were screened out by G418.The protein level and expression of hBMP 2 gene in MSCs were detected by ELISA.The transfected MSCs and BGC were cocultured to prepare composites,and the composites were transplanted into the muscle strand of rabbit's thigh.Four weeks later,the composites were taken out and tested by histological methods.RESULTS:After transfected with PcDNA3 hBMP2, about 54 ng hBMP 2 protein could be detected in the media with the ELISA method. The MSCs and BGC transfected with hBMP 2 gene were of good biocompatibility and ability of heterotopic osteogenesis. CONCLUSION:MSCs can be used as the recipient cells of hBMP 2 gene for expressing and secreting the hBMP 2 protein, and the composites of the tansfected cells and BGC were proved to have the ability of heterotopic osteogenesis, indicating that transfected MSCs by hBMP 2 protein can be served as seed cells for bone tissue engineering.

目的:观察转染了hBMP-2基因的兔骨髓基质细胞(bonemarrowstromalcells,MSC)与生物活性陶瓷(bioactiveglassceramicsics,BGC)复合后植入兔股部肌袋中生物复合材料的异位成骨能力。方法:实验时间为2003-07/2004-05。实验地点为锦州医学院解剖学骨组织工程研究所。应用脂质体介导的方法将携带hBMP-2基因的重组载体PcDNA3-hBMP2导入体外培养的MSC中,用G418筛选获得阳性细胞,应用ELISA检测hBMP-2基因在MSC内蛋白质水平的存在与表达状况;将携带hBMP-2基因的MSC与BGC复合并植入兔股部肌袋内,术后4周行组织学检察。结果:用ELISA方法从实验组细胞的培养基中可检测到约54ng的hBMP-2蛋白质。携带hBMP-2基因的MSC与BGC具有良好的生物相容性,且具有异位成骨能力。结论:MSC可以作为hBMP-2基因的受体细胞,基因表达可分泌hBMP-2蛋白质,与BGC复合后具有异位成骨能力,认为转染了hBMP-2基因的MSC可作为骨组织工程学中的种子细胞。

 
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