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   osteocalcin mrna 的翻译结果: 查询用时:0.183秒
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osteocalcin mrna
相关语句
  骨钙素mrna
     And the expression of osteocalcin mRNA was also increased by progesterone to 12.2%,23.7%and 45.5%respectively.
     孕酮增加骨钙素mRNA表达为12.2%,23.7%和45.5%。
短句来源
     2) There was expression of Osteocalcin mRNA only observed in group (EMPs+EMD).
     2 )仅EMPs+EMD组出现骨钙素mRNA的表达。
短句来源
     Osteocalcin mRNA expression was detected in 9 of 12 (75%) grade Ⅲ chondrosarcomas and 4 of 20 (20%) grade Ⅰ Ⅱ chondrosarcomas ( P =0.008).
     骨钙素mRNA表达的检测 ,在 12例Ⅲ级患者有 9例可测到 (75 % ) ,在 2 0例Ⅰ~Ⅱ级患者中有 4例可测到 (2 0 % ) (P =0 .0 0 8)。
短句来源
     Expression of Type I collagen mRAN and osteocalcin mRNA of the cells in the OGP grouop were gradually upregulated with increasing concen-trations of OGP from 10-12 -10-8 mol/L.
     在10-12-10-8 mol/L OGP范围内,其浓度较高,细胞I型胶原mRNA和骨钙素mRNA表达也较高。
短句来源
     Results: The expression of osteocalcin mRNA and the rate of proliferation of osteoblast of Group A and C were significantly higher than those of group B (P<0.01), and there was no significant difference between group A and group C (P>0.05).
     结果A、C组成骨细胞骨钙素mRNA表达量及细胞增殖率均高于B组(P<0.01),A与C组比较差异无统计学意义(P>0.05)。
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  “osteocalcin mrna”译为未确定词的双语例句
     The expression of osteocalcin mRNA in the cells treated with alcohol (0.48±0.11) was significantly lower than that in the cells treated without alcohol (1.08±0.22) ( P< 0.05 ).
     与对照组比较,实验组hBMSCs内OsteocalcinmR-NA表达降低(1.08±0.22vs0.48±0.11,P<0.05)。
短句来源
     The ALP activity was higher in the combined treatment group (2.01±0.56 U)than in the control group (1.27±0.43 U), in the inductor group(1.27±0.43 U), and in the osteoblast group(0.77±0.19 U). The osteocalcin mRNA was expressed in the three treatment groups but was not expressed in the control group.
     联合诱导组碱性磷酸酶活性为2.01±0.56U与对照组0.68±0.14U、诱导剂组1.27±0.43U及成骨细胞组0.77±0.19U比较,差异均有统计学意义(P<0.05)。
短句来源
     The ALP activity and osteocalcin mRNA expression were similar in test and control groups (P>0.05).
     实验组和对照组细胞的ALP活性及骨钙素基因表达相似,皆无显著性差异(P>0.05)。
短句来源
     The expression of PPARγ mRNA and osteocalcin mRNA in the hBMSCs of two groups was detected by RT-PCR technique.
     应用RT-PCR技术检测2组细胞中PPARγmRNA和OsteocalcinmRNA的表达。
短句来源
     Methods The expressions of typeⅠcollagen,alkaline phosphatase(ALP)and osteocalcin mRNA were determined by semiquantitative RT-PCR. Cultured cells were stained with van Gieson's staining for collagen and modified Gomori's staining for ALP.
     方法半定量RT-PCR检测MC-63细胞碱性磷酸酶(ALP)、骨钙素(OC)和Ⅰ型胶原mRNA的表达,半定量RT-PCR和Northern印迹方法检测MG-63细胞CTGF和PAIP-1 mRNA表达,改良的钙-钴法ALP染色、van Gieson法Ⅰ型胶原染色和0.1%茜素红矿化结节染色。
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  相似匹配句对
     The expression of the osteocalcin and PDGF-A mRNA were checked with RT-PCR.
     应用RT—PCR分析PDGFBB对骨钙素mRNA表达的影响。
短句来源
     Osteocalcin mRNA examination is helpful to judge the cell function status in the interface.
     骨钙素mRNA的检测有助于界面区细胞功能状态的判断。
短句来源
     The expression of mRNA increased.
     mRNA的表达增加。
短句来源
     mRNA Display Technology
     mRNA展示技术
短句来源
     ⑦ assay of osteocalcin.
     ⑦骨钙素的测定结果。
短句来源
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  osteocalcin mrna
Culture with zinc (10-5 M) caused a significant increase in α1(I) collagen mRNA expression, while it did not have a significant effect on Runx2 or osteocalcin mRNA expressions in the cells.
      
There were no significant changes in alkaline phosphatase and osteocalcin mRNA levels in response to any dose of bFGF.
      
Similar results were obtained in both KS483 osteoblasts and C2C12 myoblasts, when they were cultured with bone morphogenetic protein-2 (BMP-2) to induce osteocalcin mRNA.
      
RT-PCR using specific primers revealed that mouse bone tissues strongly expressed osteocalcin mRNA derived from OG1 and OG2, but not ORG.
      
OG1 and OG2 mRNAs were found to be expressed at ratios of 80%-86% and 14%-20%, respectively, to the total osteocalcin mRNA in mouse bone.
      
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Objective: To investigate the influence of progesterone on proliferation and differentiation of osteoblast at the levels of gene expression and cell functions. Methods: Fetal rat calvarial osteoblasts were cultured in vitro in the presence of (10 -9 mol/L ̄10 -6 mol/L) progesterone. Cell proliferation, alkaline phosphalase (ALP) activity, osteocalcin mRNA expression and osteocalcin secretion in the medium and bone nodule formation were analyzed. Results: Progesterone did not influence cell...

Objective: To investigate the influence of progesterone on proliferation and differentiation of osteoblast at the levels of gene expression and cell functions. Methods: Fetal rat calvarial osteoblasts were cultured in vitro in the presence of (10 -9 mol/L ̄10 -6 mol/L) progesterone. Cell proliferation, alkaline phosphalase (ALP) activity, osteocalcin mRNA expression and osteocalcin secretion in the medium and bone nodule formation were analyzed. Results: Progesterone did not influence cell proliferation; Progesterone enhanced the ALP activity in rat osteoblasts; Progesterone stimulated osteocalcin mRNA expression in a dose dependent manner and increased the amount of osteocalcin in the culture medium; Progesterone increased both number and area of bone nodule formation. Conclusion: Progesterone has a multi stimulating effect on the differentiation of fetal rat calvarial osteoblast, but no effect on cell proliferation.

目的:从细胞、基因水平探讨孕酮对成骨细胞增殖及分化的影响。方法:胎鼠头盖骨成骨细胞在体外经不同浓度(10-9mol/L~10-6mol/L)的孕酮作用后,对其细胞增殖、碱性磷酸酶(ALP)活性、骨钙素mRNA表达、骨钙素分泌及骨小结形成进行检测分析。结果:(1)孕酮对成骨细胞增殖无明显促进作用;(2)孕酮增加细胞ALP活性;(3)孕酮提高骨钙素mRNA表达及骨钙素的分泌,孕酮对骨钙素基因表达的刺激作用呈剂量依赖性;(4)孕酮增加骨小结形成的数量及面积。结论:孕酮对离体胎鼠头盖骨成骨细胞的分化具有多重促进效果,但对细胞的增殖无影响。

Objective To investigate the effect of progestin on the gene expression and production of osteocalcin in vitro in fetal rat calvarial osteoblasts. Methods Fetal rat calvarial osteoblasts were cultured in the medium with different concentrations of progesterone for nearly 20 days. Osteocalcin mRNA and the amount of osteocalcin in the conditioned medium were determined by northern blot and RIA analysis at different periods of culture. Results Progesterone stimulated osteocalcin gene...

Objective To investigate the effect of progestin on the gene expression and production of osteocalcin in vitro in fetal rat calvarial osteoblasts. Methods Fetal rat calvarial osteoblasts were cultured in the medium with different concentrations of progesterone for nearly 20 days. Osteocalcin mRNA and the amount of osteocalcin in the conditioned medium were determined by northern blot and RIA analysis at different periods of culture. Results Progesterone stimulated osteocalcin gene expression and production. In progesterone treated cultures the maximal expression of gene was 2 times higher than the control. The effect of the hormone was time and dose dependent. The amount of osteocalcin in the conditioned medium and the osteocalcin mRNA were increased in the middle and late periods of culture. In addition, 10 -6 mol/L progesterone acted more significantely on both gene expression and production of osteocalcin than the rest three doses of hormone. Conclusion Progesterone was involved directly in osteocalcin production by regulating gene expression and this effect of progesterone was time and dose dependent.

目的 观察孕激素对离体成骨细胞骨钙素基因表达及其合成的影响。方法 胎鼠头盖骨成骨细胞在含有不同浓度孕酮的培养基中培养约20 天。培养不同时期的骨钙素m R N A 含量及骨钙素的生成量通过 Northern 印迹杂交及放免的方法测定。结果 孕酮刺激骨钙素基因表达及其合成。其对m R N A 表达的最大作用可达对照组的2 倍以上。激素的作用呈时间及剂量依赖性。骨钙素m R N A 表达及其生成的增加见于培养的中、晚期阶段,提示孕激素对成熟的成骨细胞更具潜能。此外,所选用的4 种浓度的孕酮以浓度为10 - 6 mol/ L 时作用最明显。结论 孕酮从基因水平调节离体胎鼠成骨细胞骨钙素的生成,这种调节呈现时间及剂量依赖。

Parathyroid hormone(PTH)plays an important role in regulation of calcium and phosphorus homeostasis.Recently it has been demonstrated that PTH can also enhance hone anabolism.In osteoblast like ROS 17/2 8 cells the proliferative effect of PTH has already been observed.This report describes the differentiative effects of PTH(10 -9 and 10 -10 mol/L)that down regulated alkaline phosphates activity,increased the expression of osteocalcin mRNA,and enhanced the incorporation of 3 H proline...

Parathyroid hormone(PTH)plays an important role in regulation of calcium and phosphorus homeostasis.Recently it has been demonstrated that PTH can also enhance hone anabolism.In osteoblast like ROS 17/2 8 cells the proliferative effect of PTH has already been observed.This report describes the differentiative effects of PTH(10 -9 and 10 -10 mol/L)that down regulated alkaline phosphates activity,increased the expression of osteocalcin mRNA,and enhanced the incorporation of 3 H proline into collagen.Similar results were also demonstrated in IGF Ⅰ treated ROS 17/2 8 cells.Alkaline phosphatase,osteocalcin and collagen are three main marker proteins of differentiated osteoblast.It means that PTH and IGF Ⅰ can both regulate proliferation and differentiation at different stages of cell growth in osbteoblast like ROS 17/2 8 cells.

为了阐明甲状旁腺素(parathyroidhormone,PTH)、胰岛素样生长因子-Ⅰ(insulin-likegrowthfactor-Ⅰ,IGF-Ⅰ)在促成骨样细胞ROS17/2.8增殖的同时对其分化状况的影响,研究了PTH、IGF-Ⅰ对骨特征性标志蛋白碱性磷酸酶(alkalinephosphatase,ALP)活性、骨钙素(osteocal-cin)mRNA表达及骨胶原蛋白(colagen)合成的影响.结果表明,PTH、IGF-Ⅰ均可抑制ALP活性,PTH的抑制作用强于IGF-Ⅰ,抑制作用具有时间剂量依赖性;PTH、IGF-Ⅰ均可诱导骨钙素mRNA表达增加;PTH能够促进3H-脯氨酸参入新生小鼠颅骨组织,既PTH能够骨胶原蛋白合成增加.这些结果提示,PTH、IGF-Ⅰ在促成骨样细胞ROS17/2.8增殖的同时也促进其分化,表明在成骨细胞的生发成熟过程中,增殖、分化现象相辅相成,相伴而行.

 
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