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insecticidal crystals
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  杀虫晶体
     Analysis on the 65 kD Protoxin of Bacillus Thuringiensis Strain 4.0718 Insecticidal Crystals for Mass Spectrometry
     苏云金杆菌4.0718菌株杀虫晶体65kD原毒素的质谱分析
短句来源
     Studies on the Properties of Insecticidal Crystals from Bacillus thuringiensis Strain 4.071 8
     苏云金杆菌4.0718菌株杀虫晶体性质的研究
短句来源
     The purpose of the experiment is to use 65 kD protoxin from Bacillus thuringiensis strain 4.0718 insecticidal crystals as a model to explore a way of recovering proteins from SDS-PAGE gel for mass spectrometry.
     以苏云金杆菌 4.0 718菌株杀虫晶体 6 5kD原毒素为材料 ,探索了从SDS -PAGE胶上回收蛋白质进行质谱分析的方法。
短句来源
  晶体毒素
     Toxcity Assay and Properties of Insecticidal Crystals of Bacillus Thuringiensis Strain 4.0718
     苏云金杆菌4.0718菌株的毒力测定及晶体毒素性质的研究
短句来源
  “insecticidal crystals”译为未确定词的双语例句
     Study on Correlation in Relation to Nucleotide Sequences between 20-kb DNA in Insecticidal Crystals and Plasmids as Well as Chromosome from Bacillus Thuringiensis
     苏云金杆菌晶体中20-kb DNA与质粒及染色体的序列相关性研究
短句来源
  相似匹配句对
     Love of Crystals
     水晶之恋
短句来源
     Fractal in Crystals
     晶体中的分形(Fractal)现象
短句来源
     Studies on the Properties of Insecticidal Crystals from Bacillus thuringiensis Strain 4.071 8
     苏云金杆菌4.0718菌株杀虫晶体性质的研究
短句来源
     Toxcity Assay and Properties of Insecticidal Crystals of Bacillus Thuringiensis Strain 4.0718
     苏云金杆菌4.0718菌株的毒力测定及晶体毒素性质的研究
短句来源
     Natural Insecticidal Pyrethrum
     天然除虫菊
短句来源
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  insecticidal crystals
virescens reduced feeding and mortality caused by insecticidal crystals ofB.
      
Further, insecticidal crystals and spores of Bt exposed on plant surfaces are highly sensitive to degradation by UV light and removal by water runoff.
      


Enzyme-Linked immunosorbent assay was used to detect and quantitate the insecticidal crystal toxin proteins of B. thuringiensis HD-1 and commercial preparations. The minimal detectable value by indirect method of ELISA was 7.79 ng/0.15ml. The total crystal protein in standard sample HD-1-S-1980 and a commercial Bt emulsion (produced by Hubei Academy of Agricultural Sciences) was measured to be 108.06 mg per gram of dry powder and 4.57-6.17 mg per ml of emulsion, respectively. With these two values,...

Enzyme-Linked immunosorbent assay was used to detect and quantitate the insecticidal crystal toxin proteins of B. thuringiensis HD-1 and commercial preparations. The minimal detectable value by indirect method of ELISA was 7.79 ng/0.15ml. The total crystal protein in standard sample HD-1-S-1980 and a commercial Bt emulsion (produced by Hubei Academy of Agricultural Sciences) was measured to be 108.06 mg per gram of dry powder and 4.57-6.17 mg per ml of emulsion, respectively. With these two values, the relative toxicity potency of a commercial emulsion can then be calculated.

用酶联免疫吸附测定(ELISA)检测Bt伴孢晶体毒素蛋白质,最低可检值7.79ng/0.15ml,灵敏、精确,具高度专化性。测定标准品HI—1—S—1980和Bt乳剂产品中伴孢晶体蛋白质含量,分别为108.06mg/g粉剂和4.57—6.17mg/ml乳剂。用标准品与待测样品(Bt乳剂)的晶体蛋白质含量,就可计算出待测样品的相对毒力效价。ELISA与昆虫生测之间有良好的相关性。

Bacillus thuringiensis strain YBT-803 is highly toxic to plutella xylostella larvae. The fragment of plasmids digested wity Barn HI/PstI were analysed by southern blot using DIG-Labelled cryI gene E. coRF RNA probe. Both positive fragments of 7. 9 kh and 4. 4 kb were ligated to shuttle vector pXI 61 and transformed into competence cells E. coli TG1. The cloned strains harboring recombinant plasmids were obtained. It produced full-length 130kDa insectcidal crystal protein. The recombinant plasmid DNAs extracted...

Bacillus thuringiensis strain YBT-803 is highly toxic to plutella xylostella larvae. The fragment of plasmids digested wity Barn HI/PstI were analysed by southern blot using DIG-Labelled cryI gene E. coRF RNA probe. Both positive fragments of 7. 9 kh and 4. 4 kb were ligated to shuttle vector pXI 61 and transformed into competence cells E. coli TG1. The cloned strains harboring recombinant plasmids were obtained. It produced full-length 130kDa insectcidal crystal protein. The recombinant plasmid DNAs extracted from the cloned strains were transformed into acrystalloferous mutant Bti IPS·78/11 by electroporation. Two engineered strains with strong expression were obtained. They could form rhombus parasporal crystal and had higher toxic to plutella xylostella larvae than original strain. SDS-PAGE showed that molecular weith of insecticidal crystal proteins were 139 kDa and 65 kDa.

本文用DIG标记的cryI基因E.coRI-F片段的RNA探针,对鳞翅目高毒力菌株苏云金芽胞杆菌YBT-803质粒的BamHI/PstI酶解产物进行了Southern分析,将含有cryl基因的7.9kb和4.4kb的阳性片段与双功能载体pXI 61重组,转化感受态大肠杆菌TGl细胞,通过斑点杂交和快检获得分别含有7.9kb和4.4kb片段的克隆株,均可表达130kDa的杀虫晶体蛋白。从克隆中抽提重组质粒电转化到苏云金芽胞杆菌无晶体突变株Bti IPS·78/1l细胞中,可形成典型的菱形伴胞晶体。SDS-聚丙烯酰胺凝胶电泳表明,除了表达130kDa杀虫晶体蛋白外,还产生65kDa的多肽,它们对小菜蛾幼虫均有较高的毒力。

High conserved domain,EcoRⅠ-F fragment,of Bacillus thuringiensis insecticidal crystal protein CryⅠA gene was ligated into EcoRI site of pSELECT-1 vector.The constructed RNA probe vector, pSBL-1, could transcribe RNA at high efficiency. The RNA probe labeled by DIG transcription system in vitro had greater yield,higher sensitivity and lower background than DNA probe. It supplies an effective method for molecular biology study of Bacillus thuringiensis and screening lepidopteran-specific strains.

将苏云金芽胞杆菌杀虫晶体蛋白CryⅠA基因的高保守区EcoRI-F片段重组到pSELECT-1载体的EcoRI位点,构建出能够高效转录RNA的探针载体pSBPL-1。该载体在T7RNA聚合酶作用下,通过DIG体外标记转录制备的RNA探针,具有灵敏度高,背景清楚,省时等优点,为苏云金芽胞杆菌分子生物学研究,及对鳞翅目有特异毒性高效菌株的筛选提供了一种方便而有效的方法。

 
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