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pcr markers
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  pcr标记
     Identification of PCR Markers Linked to Wheat Powdery Mildew Resistance Gene Pm6
     小麦抗白粉病基因Pm6的PCR标记鉴定
短句来源
     3. Based on the region of previous mapping of Xa23 gene and Rice Genome Program (RGP) database, we exploited 8 new PCR markers Lj13, Lj36, Lj46, Lj55, Lj204 (Lj74), Lj210, Lj211and Lj215. Individual plants from F_2 population were surveyed using these 8 markers.
     3.开发新的PCR标记进行Xa23基因精细定位:依据国际水稻基因组计划测序的水稻品种日本晴的序列及上述定位的区域为依据设计特异引物,共开发出8个新的分子标记Lj13、Lj36、Lj46、Lj55、Lj204(Lj74)、Lj210、Lj211和Lj215。
短句来源
     1. The result of 1Dx5 and IDylO specific PCR markers was accorded with the result of SDS-PAGE in some materials, also the repetition and speciality of PCR results were good.
     1.利用5亚基和10亚基的特异PCR标记鉴定几个已知亚基组成材料的1Dx5基因型和1Dy10基因型与其SDS-PAGE验证结果相符,而且PCR标记的结果重复性好,特异性较强。
短句来源
     Tagging Salt Tolerant Gene Using PCR Markers in Soybean
     大豆耐盐基因的PCR标记
短句来源
     Out of the 22 STS_PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with Hin fⅠ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms.
     在这 2 2个STS_PCR标记中 ,仅有 3个标记的扩增产物经HinfⅠ、HhaⅠ、HaeⅢ和RsaⅠ 4种限制性内切酶消化后没有产生多态性DNA片段 ,而 19个标记 (占 86 .4 % )和 4 6种标记 /酶组合 (占 5 2 .3% )能够揭示材料间的多态性。
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  “pcr markers”译为未确定词的双语例句
     Identification of 1BL/1RS Translocation via Multiplex PCR Markers of Glu-B3, Gli-B1 and SEC-1bin Common Wheat
     用Glu-B3、Gli-B1和SEC-1b复合引物PCR检测普通小麦1BL/1RS易位系
短句来源
     Development of PCR Markers Linked with Powdery Mildew Resistant Genes Pm4 and Pm6 in Wheat
     小麦抗白粉病基因Pm4、Pm6的PCR鉴定
短句来源
     【Method】 The composition and the sources of the stripe and stem rust resistance genes of YW243 were analyzed by using PCR markers of Yr9, YrX, Yr2, Yr1, Sr31 and SDS-PAGE.
     【结果】YW243含有抗条锈病基因Yr1、Yr2、Yr9、YrX,抗秆锈病基因Sr31、抗叶锈病基因Lr26。 Yr1源于亲本陕7859或丰抗13,Yr2源于陕7859或丰抗8号或丰抗13;
短句来源
     Development of New PCR Markers Specific to a Thinopyrum intermedium Chromosome 2Ai-2 and Cloning of the St-specific Sequences
     中间偃麦草染色体2Ai-2特异PCR新标记的建立和St基因组特异序列的克隆
短句来源
     After the linkage analysis between these PCR markers and the resistance gene among F 2 population plants of HW642/Zhong8601, the results indicated that gwm37 -450 and SC W37 450 were closely linked to the BYDV resistance gene and the SCAR marker SC W37 450 was more reliable and easily scored.
     利用 HW6 4 2 /中 86 0 1的 F2 群体 ,对 gwm 37- 450 和 SC- W374 50 进行分析 ,结果表明 ,二者均与抗黄矮病基因紧密连锁 ,后者较前者稳定。
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  相似匹配句对
     -PCR.
     RT-PCR检测COXⅠ和COXⅣmRNA稳态量(statelevel)。
短句来源
     -PCR.
     pylori-UreA-PCR技术进行H.
短句来源
     Amplification of Pig Microsatellite Markers Using Multiplex PCR
     猪微卫星标记多重PCR扩增组合
短句来源
     Studies on the identification of pennisetum hybrids by PCR-RAPD markers
     RAPD分子标记在鉴定狼尾草属杂交后代上的应用
短句来源
     were its chromosome markers.
     等为其标志染色体,未显示有完整Y染色体存在。
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  pcr markers
Sixty-nine Alu-PCR markers, generated from the YACs, and seven PCR primers were used to screen for overlaps between individual clones.
      
A total of 299 PCR markers (67 SSRPs and 232 AFLPs) were detected in eight populations, of which 98.7% were polymorphic markers.
      
We successfully combined two pairs of them and characterized two multiplex PCR markers which could discriminate the homozygous and heterozygous genotypes.
      
PCR markers for mutated S-haplotypes enable discrimination between self-incompatible and self-compatible sour cherry selections
      
With this approach, 24 (71%) of the 34 selected RFLPs were converted into simple PCR markers.
      
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Molecular marker-based selection in plant breeding requires not only suitable molecular markers closely linked to the known genes,but a simple,economic and reliable analyzing technique.We report here a useful PCR marker for genetic diagnostics in breeding for resistance to rice bacterial blight.Xa21 is a newly found gene of rice which confers resistance to bacterial blight caused by Xanthomonas oryzae pv.oryzae.We produced two F2 populations between one resistant line IRBB21 containing Xa21...

Molecular marker-based selection in plant breeding requires not only suitable molecular markers closely linked to the known genes,but a simple,economic and reliable analyzing technique.We report here a useful PCR marker for genetic diagnostics in breeding for resistance to rice bacterial blight.Xa21 is a newly found gene of rice which confers resistance to bacterial blight caused by Xanthomonas oryzae pv.oryzae.We produced two F2 populations between one resistant line IRBB21 containing Xa21 and two susceptible varieties,respectively.One PCR marker,PB78,detected polymorphism between the susceptible varieties and the resistant line.Cosegregation between Xa21 and PB78 was studied in the two F_2 populations.The results showed that Xa21 was closely linked to the molecular marker,the crossing over value was 2.48%.Marker-based selection revealed that 100% of the plants with homozygous resistant genotype of PB78 showed resistance to bacterial blight.The available approaches detecting molecular markers in plant breeding are also discussed.

植物育种中应用分子标记辅助选择要求分子标记与目的基因紧密连锁,而且分析手段经济简便、重复性好。Xa21是最近发现的一个具有广谱抗性的水稻白叶枯病抗性基因,利用一个含Xa21基因的品系IRBB21分别与2个感病品种杂交获得2个F_2群体。用4对引物分别对3个亲本进行PCR分析,其中一对引物(PB78)的PCR产物在抗、感病品种间可揭示多态性。对2个F_2群体进一步的遗传分析表明,PCR标记和Xa21的白叶枯病抗性紧密连锁,其重组率仅为2.48%。根据该标记选择基因型纯合的抗性植株,其准确率可达100%。本文还就植物育种中分子标记的检测途径进行了评价。

Soybean Molecular Biology Laboratory at Institute of Crop Germplasm Resources CAAS, has been carrying on several research projects including the molecular tagging important genes, genetic diversity of germplasm and markers assisted selection in soybean during the ninth-five-year. So far, the PCR marker has been identified to be associated with salt tolerant gene in soybean, which was applied for patent in China. The important progress also has been made in identification of molacular markers...

Soybean Molecular Biology Laboratory at Institute of Crop Germplasm Resources CAAS, has been carrying on several research projects including the molecular tagging important genes, genetic diversity of germplasm and markers assisted selection in soybean during the ninth-five-year. So far, the PCR marker has been identified to be associated with salt tolerant gene in soybean, which was applied for patent in China. The important progress also has been made in identification of molacular markers associated with SMV resistant gene, evaluation genetic diversity for soybean germplasm, application of molecular markers in soybean breeding and generating novel germplasms.

中国农业科学院品种资源研究所大豆分子生物学实验室“九五”期间承担了大豆重要基因的分子标记和利用生物技术创造农作物特异新种质等国家科技攻关项目。经过三年的研究,已完成大豆耐盐性基因的分子标记,并申请了国家专利。在抗大豆花叶病毒病(SMV)基因标记、大豆遗传多样性评价、分子标记辅助育种和种质创新等方面也取得重要进展。

Hybrid cotton is extensively grown in China. Hybrid seeds are generally planted and identified for the purity in Hainan province. It takes time and costs much money. In the paper, molecular marker is explored to make the purity test for the hybrid cotton for the first time in cotton. It is shown that it is easy to screen RAPD-PCR markers to distinguish the F 1 hybrids from their parents. Molecular markers to inspect the purity of a hybrid cotton, Wangza 40, which is grown extensively...

Hybrid cotton is extensively grown in China. Hybrid seeds are generally planted and identified for the purity in Hainan province. It takes time and costs much money. In the paper, molecular marker is explored to make the purity test for the hybrid cotton for the first time in cotton. It is shown that it is easy to screen RAPD-PCR markers to distinguish the F 1 hybrids from their parents. Molecular markers to inspect the purity of a hybrid cotton, Wangza 40, which is grown extensively in Anhui Province, are presented. It can be easily used to measure the purity of the hybrid cotton quickly.

分子标记技术已经被广泛地用于棉花的遗传育种研究中。本研究利用RAPD- PCR技术,对我国现有的皖杂40 杂种棉组合的纯度测验进行了初步研究。研究结果表明,利用OPW09 引物可以很容易地把皖杂40 F1 杂种和它们的亲本加以区别;棉花杂种的纯度可以很快的加以鉴定;从而避免每年冬天去海南的异地鉴定,节省时间和杂种种子的成本。

 
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