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restriction fragment
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  限制性片段
     Methods :In 122 healthy controls and 92 patients with type 2 diabetes, to analyze the Gly15Gly genotypes of adiponectin gene exon 2 by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), and compare the frequencies of genotype and allele in two groups.
     方法 :采用聚合酶链反应 -限制性片段长度多态法 (PCR -RFLP)检测122名正常对照者和92名2型糖尿病患者脂联素基因外显子2Gly15Gly基因型 ,比较两组基因型和等位基因频率。
短句来源
     GENOMIC DNA FINGERPRINTING BY RESTRICTION FRAGMENT END LABELING
     末端标记限制性片段的基因组DNA指纹法
短句来源
     Polymerase chain reaction followed by restriction fragment length polymorphism(PCR-RFLP) technology was used for detecting the single nucleotide polymorphisms(SNPs)of Msp I in the non-coding region of CYP-1A1 gene and c . 188 ,g . 212 position in the first extron of CYP2D6 gene.
     采用限制性片段聚合酶链反应(PCR—RFLP)技术榆测CYP1A1基因3’端非编码区Msp I和CYP2D6第1外显子c.188位点、g.212位点多态性。
短句来源
     Methods The point mutations of H ras and K ras atcodon 12 were detected in 36 GTDs as well as in the JAR,BeWo Choriocarcinoma cell lines by a polymerase chaine reaction restriction fragment length polymorphisms (PCR RFLP);
     方法应用PCR-RFLP(聚合酶链反应-限制性片段长度多态分析)检测绒毛膜细胞株JAR和BeWo、和36例标本(6例新鲜组织及30例石蜡标本)中H-ras、K-ras的第12位点点突变情况;
短句来源
     MethodsPolymerase chain reaction and technique of restriction fragment length polymorphism were used to detect the genotypes of Apo H exon 3 and exon 8 in 110 patients with CHD and 100(healthy) subjects.
     方法采用聚合酶链式反应结合限制性片段长度多态分析方法,分析了100例健康人及110例冠心病病人的Apo H外显子3、8基因型,并对血脂进行了测定。
短句来源
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  限制性片断
     For the PCR products of point 3243 and 8993,restriction fragment length polymophism(RFLP)was performed to search for A3243G、T8993G or T8993C point mutations.
     对点3243、8993的PCR产物采用限制性片断长度多态性(RFLP)分析法检测该片断中有无A3243G、T8993G或T8993C点突变;
短句来源
     Methods: APOE-219G/T genetype in AMD group(50 cases)and control group(95 persons)was identified by polymerase chain reaction and restriction fragment length polymorphism genotyping technique.
     方法:用聚合酶链反应-限制性片断长度多态性分析法对AMD组(50例)和对照组(95例)的APOE-219G/T基因型做出鉴定。
短句来源
     Association of XbaⅠ restriction fragment length polymorphism(RFLP)of estrogen receptor(ER)gene and bone mineral density in postmenopausal women.
     绝经后妇女雌激素受体(ER)基因XbaⅠ限制性片断长度多态性(RFLP)与骨密度的关系
短句来源
     CYP2D6*10 genotypes of patients were assayed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
     采用聚合酶链反应(PCR)和限制性片断长度多态性(RFLP)测定CYP2D6*10基因型.
短句来源
     (4) The A/G polymorphism at position 49 in exon 1 of the CTLA-4 gene was analyzed with technique of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
     (4)用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术分析CTLA-4外显子1的49位点基因型。
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  限制片段
     The loss of heterozygosity (LOH) at chromosomes 3p24 in 16 Xinjiang Kazak patients with esophageal cancer was detected by means of polymerase chain reaction (PCR), restriction fragment length polymorphisms (RFLP) analysis, and compared Kazak's, Han's and Uygur's LOH.
     应用聚合酶链反应(polymerase chain reaction,PCR)技术,配合限制片段长短多态现象(restriction fragment length polymorphisms,RFLP)分析,对16例新疆哈萨克族食管癌病人3号染色体短臂3p24等位基因杂合缺失(loss of heterozy-gosity,LOH)进行了测定,并同汉族、维吾尔族食管癌病人3p24杂合缺失进行了比较.
短句来源
     Methods PCR amplification of HBV small S gene in combination with a restriction fragment length polymorphism (RFLP) analysis was applied to identify HBV genotype in the serum samples of 80 asymptomatic HBV carriers (AsC group) and 120 liver cirrhosis patients with HBV infection (LC group) in Gangdong province of China.
     方法 用S基因聚合酶链式反应 -限制片段长度多态性 (PCR -RFLP)的基因型分型方法对广东地区HBV感染者中无症状携带者 (AsC)组 80例和肝硬化组 (LC) 12 0例血清标本分型 ,比较两组基因型分布的异同。
短句来源
     Methods Using polymerase chain reaction restriction fragment length polymorphisms(PCR RFLP) techniques,the genotypes' polymorphism of MPO gene in 35 patients with chronic benzene poisoning,46 workers exposed to benzene from the same workplace(as exposed control) and 26 controls were analyzed.
     方法 采用多聚酶链反应 -限制片段长度多态性 (PCR RFLP)技术和DNA测序法 ,分析 35例慢性苯中毒患者、46名苯作业工人和 2 6名健康工人的MPO基因转录起始位点上游 463位点等位基因分布情况 ,比较各组间的差别 ,探讨MPO基因多态性与苯中毒危险性的可能关联。
短句来源
     Methods Polymerase chain reaction sequence specific primers (PCR SSP) technology was used to determine human leukocyte antigen (HLA) DR and DQ alleles in 32 patients with CHB. Serum HBV DNA level was quantified by a PCR assay with a low limit of detection of 1000 copies/ml. HBV genotypes, precore(A 1896 ) and core promoter(T 1762 A 1764 ) mutations was determined by PCR restriction fragment length polymorphism(PCR RFLP) analysis.
     方法 以聚合酶链反应 序列特异性引物 (PCR SSP)技术检测 32例接受IFN治疗CHB患者人类白细胞抗原 (HLA) DR和 DQ等位基因 ,以荧光PCR技术定量检测HBVDNA水平 ,以PCR 限制片段长度多态性 (PCR RFLP)分析检测HBV基因型和基因组变异 (A1896突变和T1762 A1764突变 ) ,并分析上述各因素对IFN应答率的影响。
短句来源
     Objective To establish restriction fragment differential display polymerase chain reaction (RFDD-PCR) as an efficient technique for constructing and studying gene expression profile of human tissues.
     目的 探讨用限制片段差异显示聚合酶链反应(Restriction Fragment Differential Display-Polymerase Chained Reaction,RFDD-PCR)技术建立基因差异表达谱的方法,及其应用于高通量分析人类疾病差异表达谱的可行性。
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  “restriction fragment”译为未确定词的双语例句
     THE HOMODUPLEX BETWEEN ~λ DNA AND ITS EcoR1 RESTRICTION FRAGMENT ANE THE HETERODUPLEX BETWEEN ~λDNA AND λPLAC5 DNA
     ~λDNA与其EcoR1酶切片段的同源双链以及与~λplac 5 DNA的异源双链
短句来源
     Cloning Promoter Containing DNA Restriction Fragment in Bacillus subtilis
     带有启动子DNA的片段在枯草芽孢杆菌中的克隆
短句来源
     RESTRICTION FRAGMENT LENGTH POLYMORPHISMS ON Xp21 REGION OF THE X CHROMOSOME IN NORMAL CHINESE AND DMD PATIENTS
     正常中国人和DMD患者X染色体Xp~(21)区的RFLP研究
短句来源
     A Study of Restriction Fragment Length Polymorphisms in Human Homeobox Hu-1 Containing Gene
     人同源异型盒基因Hu-1位点的RFLPs研究
短句来源
     STUDY ON RESTRICTION FRAGMENT LENGTH POLYMORPHISMS OF ST14/TAQ I IN CHINESE AND THEIR APPLICATION TO PRENATAL DIAGNOSIS OF HAEMOPHILIA A
     中国人St14/Taq I RFLPs及其在产前诊断中的应用
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  restriction fragment
A polymerase chain reaction-restriction fragment length polymorphism analysis was used to discriminate isolates of Bursaphelenchus xylophilus and B.
      
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype GNB3 C825T polymorphism in 354 hypertensive (HT) and 384 normotensive (NT) Uygur subjects.
      
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis for 13 restriction sites, including BamHI, EcoRV, Sau3AI (one site each), KpnI (two sites), HaeIII (three sites), and RsaI (five sites) were used.
      
strains SN11, SN101, and G51 are conjugative, contain genes responsible for the degradation of naphthalene and salicylate, and are characterized by the same restriction fragment maps.
      
A restriction fragment length polymorphism analysis of the IGS2 rDNA region reveals two different AluI profiles, one of which corresponds to Kl.
      
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Restriction endonuclease EcoR1and BamH 1 were used to producefragments pBR 322 C (375 bp) andpBR 322 B (3987 bp) from pBR 322and to produce λF_2A (65.6—71.3%of λ DNA,2967 bp) and λF_2B (71.3—81% of λDNA,4559 bp) fromEcoR1 restriction fragment λF_2(65.6—81% of λDNA) of λcI_(857)S_7DNA.By recombining pBR 322 B andλF_2B in vitro,a new plasmid calledpCB 2 carrying λ promoters and struc-tural genes cI and cro was constructed.The desired strain with pCB 2 wasselected from 338 transformants forits Ap~rTc~s and...

Restriction endonuclease EcoR1and BamH 1 were used to producefragments pBR 322 C (375 bp) andpBR 322 B (3987 bp) from pBR 322and to produce λF_2A (65.6—71.3%of λ DNA,2967 bp) and λF_2B (71.3—81% of λDNA,4559 bp) fromEcoR1 restriction fragment λF_2(65.6—81% of λDNA) of λcI_(857)S_7DNA.By recombining pBR 322 B andλF_2B in vitro,a new plasmid calledpCB 2 carrying λ promoters and struc-tural genes cI and cro was constructed.The desired strain with pCB 2 wasselected from 338 transformants forits Ap~rTc~s and for its immunity to λ infection.pCB 2 was characterized by:1.its genetic marker Ap~rTc~s,because BamH1 site is right in theTc region of pBR 322,so as to renderit Tc~s.2.transforming C 600 to be im-mune to λcIs857-S7 infection,since λF_2B contains immune regionof this λDNA,with cI and cro geneswhich are expressed under the direc-tion of the λpromoters to producerepressor and cro protein respectivelyand thus plaque formation is preven-ted.This shows that the promotersin our pCB 2 are working and thatcI or/and cro gene in λDNA fragmentis expressed in E.Coli.Moreover,the gene product which is responsiblefor the immunity to λinfection isthermostable.3.having the length of 2.66±0.33μ and MW of 5.51±0.68×10~6dalton (calculated MW=5.41×10~6dalton) estimated by electron mi-croscope and gel electrophoresis afterdigestion with either EcoR 1 orBamH 1.4.giving rise to 2 molecules ofdifferent MW.after digestion withboth EcoR1 and BamH 1.The largeone corresponds to λF_2B while thesmall one corresponds to pBR 322 B.This excludes pCB 2 from beingpBR 322 B dimer or λF_2B dimer whichshould give rise to 2 identical mole-cules (ie.pBR 322 B or λF_2B re-spectively) upon digestion with thesame two enzymes.Further more,the bacterial harboring pBR322Bdimer should show no immunity tothe infection with phage λ,whileλF_2B dimer should be Ap~sTc~s in-stead of Ap~rTc~s.5.its transforming activity.Thefrequency of transformation is 2.1×10~6CFU/μg DNA.6.giving a heteroduplex afterdenaturation and renaturation of λF_2and pCB 2 together.The lengthsmeasured for the single stranded partsand double stranded part agree wellwith our original design.From the above characteristics ofpCB 2,we come to the conclusion thatpCB 2 we constructed is a new plas-mid with cI and/or cro gene expressedunder the direction of λ promoters.

我们用EcoR1及BamH1两种限制性核酸内切酶来酶解质环pBR322成为pB-R322C(375bp)及pBR322B(3987bp),并酶解λcI857S_7DNA 的EcoR1限制片段λF_2(λDNA 的65.5—81%片段)成为λF_2A(λDNA 的65.6—71.3%片段,2679bp)及λF_2B(λDNA 的71.3—81%片段,4559bp)。再用T_4-DNA 连接酶将pBR322B 及λF_2B重组成为一个新质环,叫做pCB_2,其上具有cI 基因,cro 基因及启动基因,这是从随机挑取的338个菌落的第48号菌所携带的新质环。pCB_2的性质是:1.它赋予转化子以单抗药性(Ap~r),2.它赋予转化子对λcI857S_7感染的免疫性,由此可见,我们接上去的λ启动基因在发挥作用,cI 基因或/和cro 基因得以表达。第48号菌经过42℃处理后,其免疫能力仍不低于处理前的。3.经EcoR1或BamH1一种酶水解后,用电镜及凝胶电泳检查,测得pCB_2的分子长度为2.66±0.33μm,分子量为5.51±0.68×10~6d(计算值为5.41×10~6d,8546bp)。4.经过EcoR1...

我们用EcoR1及BamH1两种限制性核酸内切酶来酶解质环pBR322成为pB-R322C(375bp)及pBR322B(3987bp),并酶解λcI857S_7DNA 的EcoR1限制片段λF_2(λDNA 的65.5—81%片段)成为λF_2A(λDNA 的65.6—71.3%片段,2679bp)及λF_2B(λDNA 的71.3—81%片段,4559bp)。再用T_4-DNA 连接酶将pBR322B 及λF_2B重组成为一个新质环,叫做pCB_2,其上具有cI 基因,cro 基因及启动基因,这是从随机挑取的338个菌落的第48号菌所携带的新质环。pCB_2的性质是:1.它赋予转化子以单抗药性(Ap~r),2.它赋予转化子对λcI857S_7感染的免疫性,由此可见,我们接上去的λ启动基因在发挥作用,cI 基因或/和cro 基因得以表达。第48号菌经过42℃处理后,其免疫能力仍不低于处理前的。3.经EcoR1或BamH1一种酶水解后,用电镜及凝胶电泳检查,测得pCB_2的分子长度为2.66±0.33μm,分子量为5.51±0.68×10~6d(计算值为5.41×10~6d,8546bp)。4.经过EcoR1及BamH1二种酶水解后,分解为二种分子,一大一小。在凝胶电泳中,其分子量大的与λF_2B 相当,小的与pBR322B 相当。5.pCB_2具有生物活性,其转化频率为2.1×10~6CFU/μgDNA。6.用λF_2与经历EcoR1切割成线状的pCB_2在一起进行变性后复性,在电镜下观察异源双链图形,其长度与建造时的设计相符。从以上结果我们得出的结论是:我们建造的PCB_2是一个新质环,其中的CI 基因及/或cro 基因,能在λ启动基因指导下,在大肠杆菌中表达。

The DNA molecules were pre-pared for electron microscopy by the basic protein film-formamide spread-ing method.The lengths of the linear double stranded λ DNA and its EcoRl restriction fragments were measured from the electron micrographs.The homoduplex molecule between A DNA and its three EcoRl restriction fragments was prepared at the molar ratio of 1:22.The lengths of the double stranded regions of the three homoduplex mo-lecules agreed with those of the F_1 F_3,F_4,fragments respectively,and...

The DNA molecules were pre-pared for electron microscopy by the basic protein film-formamide spread-ing method.The lengths of the linear double stranded λ DNA and its EcoRl restriction fragments were measured from the electron micrographs.The homoduplex molecule between A DNA and its three EcoRl restriction fragments was prepared at the molar ratio of 1:22.The lengths of the double stranded regions of the three homoduplex mo-lecules agreed with those of the F_1 F_3,F_4,fragments respectively,and both ends of all double stranded re-gions were flanked by the single stra-ded regions.The total length of the heteroduplex molecule between A DNA and λ plac 5 DNA was about the same as that of the A DNA molecule and a single stranded loop was located between 41.5-47.2% of the molecule.

用硷性蛋白膜甲酰胺铺展开法对DNA分子进行电镜制样,拍摄了线状双链λDNA和其ECOR 1酶切片段的电镜照片,并测得它们的长度。λDNA与其三个EcoR 1酶切片段的同源双链分子是以1:22克分子比例制得的,三个同源双链分子的双链长度分别与F_1、F_3、F_4片段的长度相同,两端均为单链。λDNA与λplac5 DNA的异源双链分子总长度与λDNA大致相同,单链环位于分子的41.5—47.2%之间。

Using the plasmid pBR322 as a vector, EcoRI-BamHI and BamHI restriction fragments of the ribosomal RNA gene (rDNA) from the silk worm Attacus ricini (Philosamia cynthia ricini) have been cloned in E. coli. The agarose gel electrophoretic analysis of restriction fragments from hybrid plasmid pAR1 and pAR2 and Southern hybridization indicated that pAR1 and pAR2 contain respectively a 1.95Md EcoRI-BamRI fragment and a 2.6Md BamHI fragment. The direction of rDNA BamHI 2.6 Md inserted fragment...

Using the plasmid pBR322 as a vector, EcoRI-BamHI and BamHI restriction fragments of the ribosomal RNA gene (rDNA) from the silk worm Attacus ricini (Philosamia cynthia ricini) have been cloned in E. coli. The agarose gel electrophoretic analysis of restriction fragments from hybrid plasmid pAR1 and pAR2 and Southern hybridization indicated that pAR1 and pAR2 contain respectively a 1.95Md EcoRI-BamRI fragment and a 2.6Md BamHI fragment. The direction of rDNA BamHI 2.6 Md inserted fragment has been determined.

利用质粒pBR322作运载体,获得了蓖麻蚕(Attacus ricini)核糖体rRNA基因(rDNA)的部分片段在E.coli中的无性繁殖株。酶切图谱分析及Southern法分子杂交鉴定证明,重组质粒pARI含有1.95MdrDNA EcoRI-BamHⅠ双酶切片段;pARⅡ含有2.6Md的rDNABamHⅠ片段。并测定了BamHⅠ片段与pBR322连接方向。

 
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