The detection limit of the method was 13 ng/ml for 4-NP and 4 ng/ml for BPA. Recoveries of rat serum samples were 83.8%-100.0% for 4-NP and 83.3%-100.0% for BPA.

Results There was good linear relationship between the concentrations of the analytes in rat serum and their peak areas in the ranges of 0.013-5.0 μg/ml for 4-NP and 0.004-3.0 μg/ml for BPA.

This paper use yeast estrogen screen to study the estrogenic effect of individual andcombination on 17β-estradiol (E2), 17α-ethynylestradiol (EE2), 4-n-Nonylphenol (4-NP),Bisphenol A (BPA), Pentachlorophenol (PCP), o,p’-Dichlorodiphenyltrichloroethane(o,p’-DDT), and p,p’-Dichlorodiphenyl- trichloroethane (p,p’-DDT).

Male Carassius auratus received intraperitoneal injections with a dose series of 17β-estradiol(E_2)(0.01,0.10 and 1.00 mg/kg; single injection) and 4-NP(1,10,50,100 and 200 mg/kg;single injection). The estrogenic effects of the treatment were investigated on vitellogenin measured by enzyme linked immunosorbent assay(ELISA) at different time points.

The concentrations of 4-nonylphenol(4-NP),1,1-dichloro-2,2,bis(p-chlorophenyl)ethylene(p,p′-DDE) and di-2-ethylhexyl phthalate(DEHP) in blood serum samples were measured by using reversed-phase high performance liquid chromatography(HPLC).

These results indicate that these two substances must be controlled and their water quality standards should be: ρ (4-NP)≤0.014 mg/L, ρ (NP_ 10 EO)≤ 0.60 mg/L.

A simple and highly sensitive method has been developed for the determination of (bisphenol) A (BPA) and 4-nonylphenol (4-NP) in rat tissues by high performance liquid chromatography (HPLC) with fluorescence detection.

Recoveries of serum samples were 96.84%-103.44% for DEHP, 87.69%-104.92% for MEHP,87.6%-109.89%for p,p′-DDE,98.57%-105.1% for 4-NP. Conclusion The method is suitable for the determination of the four kinds of EEDs in serum. It is easy to dispose the samples.

The RLS detection was performed at 396.8 nm. Under these conditions, 4-NP was showed good linearity in the range of 6.67×10~(-4)~6.00×10~(-2)μg/ml with correlation coefficient 0.9990, and the limit of detection was 0.200ng/ml.

However, of the proteins induced by either E2 or 4-NP, 28% were common to both chemicals at 1?ppm but only 7% were common to both at 0.1?ppm.

In both experiments approximately 30% of the proteins sampled were specific to either E2 or 4-NP and about 33% were common to the control, 4-NP and E2.

Embryonic zebrafish were examined for changes in protein expression following exposure to sublethal concentrations of 17β-estradiol (E2) and the estrogen mimic 4-nonylphenol (4-NP).

A largemouth bass (LMB) array containing 132 genes has been designed to study the impact of gene expression in male fish exposed to 17-β estradiol or to the compounds 4-nonylphenol (4-NP) or 1,1-dichloro-2, 2-bis (p-chlorophenyl) ethylene (p,p'-DDE).

In a complementary experiment, extensive metabolism of [3H]4-n-NP was characterized following in vivo exposure of juvenile mosquitofish suggesting that metabolism could modulate 4-NP toxicity.

A more general mathematical analysis of the kinetics of polycondensation-depolycondensation reactions is given in the article. Three different types of reactions are considered, namely, (1) reactions between molecules AB and AB, (2) reactions between molecules AA and BB, (3) reactions between molecules AA and BC. One of the condensation products is assumed (without loss of generality) to be water.For reactions of the first type, [p_n], the number of molecules of the n-mer (AB)_n, obeys the following Flory distribution:...

A more general mathematical analysis of the kinetics of polycondensation-depolycondensation reactions is given in the article. Three different types of reactions are considered, namely, (1) reactions between molecules AB and AB, (2) reactions between molecules AA and BB, (3) reactions between molecules AA and BC. One of the condensation products is assumed (without loss of generality) to be water.For reactions of the first type, [p_n], the number of molecules of the n-mer (AB)_n, obeys the following Flory distribution: [p_n]=N_0p~(n-1)(1-p)~2 where N_0 denotes the total number of AB segments (including the unreacted monomers). and p, as defined by is a measure of the degree of condensation. It is shown that p is the solution of the following differential equation: dp/dt=k/2N_0(1-p)~2-k_(-1)p[H_2O] where K and k_(-1) are velocity constants of condensation and hydrolysis respectively, and [H_2O] denotes the number of water molecules. Three special cases are discussed.For reactions of the second type, three different types of condensation products are possible besides water; they obey the following distributions: [p_(2n)]=2N′_0r~(n-1)p~(2n-1)(1-p)(1-rp) [p′_(2n-1)]=N′_0r~(n-1)p~(2n-2)(1-p)~2 [p″_(2n-1)]=N′_0r~(n-2)p~(2n-2)(1-rp)~2 where N′_0 and N″_0 denote the total number of segments AA and BB respectively, r denotes the ratio N′_0/N″_0, and p, as defined by is the solution of the following differential equation: dp/dt=kn′_0/r(1-p)(1-rp)-k_(-1)P[H_2O]For reactions of the third type, six different types of condensation products are possible besides water; they obey the following distributions: [P′_(2n)]=NP~(n-1)q~n(1-p/2-q/2)(1-p) [p″_(2n)]=Np~nq~(n-1)(1-p/2-q/2_(?))(1-q) [p_(2n-1)=Np~(n-1)q~(n-1)(1-p/2-q/2)~2 [p′_(2n-1)]=N/4p~(n-2)q~n(1-p)~2 [p″_(2n-1)]=N/4p~nq~(n-2)(1-p)~2 [p′″_(2n-n)]=∈(n)N/4p~(n-1)q~(n-1)(1-p)(1-q),where N denotes either the total number of segments AA or that of BC, while p and q, as defined by satisfy the following set of differential equations: dp/dt=k′N/2(1-p)(2-p-q)-k′_(-1)p[H_O] dq/dt=k″N/2(1-q)(2-p-q)-k″_(-1)q[H_2O]

A synchronised accumulation technique is used to achieve a resolution of up to 10-13 cm for the oscillating amplitude of a laser interferometer. Such a technique is applied to observe the 60.4 Hz gravitational radiation which is possibly emitted from the pulsar NP 0532 of the Crab Nebula. Present experiments show that no such signal is received.

In this paper", the further development of a new type of general-purpose Supercomputer- "Cellular Vector Computer of Vertical and Horizontal Processing" (CVCVHP) from "with Common Memory "to "with Virtual Common Memory", is diseussed. The subsystem of "CVCVHP with Virtual Common Memory" is equivalent to a multidimentional array processor.Starting from the "Vector Computer of Vertical and Horizontal Processing" (m×np type) based on small and medium scale integrated circuits, we briefly describe "CVCVHP...

In this paper", the further development of a new type of general-purpose Supercomputer- "Cellular Vector Computer of Vertical and Horizontal Processing" (CVCVHP) from "with Common Memory "to "with Virtual Common Memory", is diseussed. The subsystem of "CVCVHP with Virtual Common Memory" is equivalent to a multidimentional array processor.Starting from the "Vector Computer of Vertical and Horizontal Processing" (m×np type) based on small and medium scale integrated circuits, we briefly describe "CVCVHP with Common Memory" (m X n type, m × np type), which is introduced because of the development of very large scale integrated circuits. This, is a new type of vector computer which employs a multiple data stream and multiple instruction stream architecture.On this basis, for the sake of raising the speed of a system by largely increasing the number of cells, in this paper we emphasize a new type of supercomputer, i.e. CVCVHP with "Virtual Common Memory in Addition to Common Memory". This system may consists of thousands of cells. And we conclude that its subsystem is equivalent to a multidimentional array processor. Seen from another angle, we propose a class of methods of using a supercomputer which consists of thousands of "microprocessors" (more exactly, the "cells").The system presented also has such features: from the view of physical organization it appears as a multidimentional array processor system, but from the view of function (i.e. the view of users) it is a vector computer. The memory is distributed physically, but is concentrated from the view-point of users.The following problems of this new type of supercomputer system are also discussed: language, principal algorithms, corresponding principal instructions, principal functions of the cell, '' Common Memory'', synchronizing, the number of data transmission lines, the number of transmission steps, and so on.Finally, we consider a simplified system, the language of which is completely consistent with the "High Level Vector Language" of a normal vector computer.The system we propose in this paper may constitute a series of computer complex of various sizes.