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residual dna
相关语句
  残余dna
     The residual DNA and calf serum in vaccine were <100pg/0. 5ml and <1μg/ml, respectively.
     Vero细胞残余DNA<100pg/0.5ml,残余牛血清<1μg/ml。
短句来源
     The recovery rate,biological activity and specific activity of purified bFGF were 40% , 0.21ng/ml and 4.76 × 10 U/mg respectively, and the purity, relative molecular weight, residual DNA of it met The Requirements for Quality Control of Recombinant DNA Preparation.
     提纯的bFGF回收率为40%,生物学活性(ED50)为 0.21ng/mL,比活性达4.76×106U/mg,其纯度、分子量、残余DNA等各项检测结果均符合“重组 DNA制品质量控制要点”。
短句来源
     The detection of the residual DNA in the biological products by labeled probe of digoxigenin
     地高辛标记探针检测生物制品中残余DNA含量
短句来源
     Determination of Residual DNA in Recombinant Cytokines by Fluorescence Method
     应用荧光法测定重组细胞因子中残余DNA含量
短句来源
     Semi-quantitative Analysis of the Residual DNA in r-hTNFαDerivation by DIG Labeling DNA Probs Hybridization
     地高辛标记探针检测重组人肿瘤坏死因子α衍生物中残余DNA
短句来源
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  dna残留量
     Analysis for Residual DNA in Recombinant Double Functions Hirudin Using Digoxin Labeled Probe
     地高辛标记探针检测重组双功能水蛭素中的DNA残留量
短句来源
     DNA of engineering bacteria of recombinant double functions hirudin were labeled by Digoxin and used as probe for dot hybridization to detect residual DNA in semi-finished products of recombinant double functions hirudin for injection. At the same time, specificity and sensitivity experiments were performed.
     为检测注射用重组双功能水蛭素半成品中的DNA残留量 ,应用地高辛标记重组双功能水蛭素工程菌DNA ,并以此为DNA探针进行点杂交 ,同时做特异性及灵敏度实验。
短句来源
  “residual dna”译为未确定词的双语例句
     Study on Removing Residual DNA of Vero Cells in OPV with Protamine Sulphate
     硫鱼精蛋白去除OPV中Vero细胞基质DNA的研究
短句来源
     The technique of DNA hybridization was applied to detect exogenous residual DNA in rIL-2 productrs with 32 P as a probe.
     采用DNA杂交技术,用32P标记的探针检测重组白细胞介素2制品的外源DNA残余量。
短句来源
     Detection of residual DNA from E. coli in recombinant human granulocytic-marcophagic colony stimulating factor/interleukin-3 fusional protein
     rhGM-CSF/IL-3融合蛋白中残余菌体DNA的检测
短句来源
     The repair rate of DNA damage in WM9839 cells after 2 Gy fast\|neutron irradiation was lower than that after 2 Gy γ\|ray irradiation. The residual DNA damage at 180 min after neutron\|irradiation was obviously severer than that after 2 Gy γ\|ray irradiation.
     快中子 2Gy照射后 ,WM 9839细胞DNA损伤修复曲线整体上下降较γ射线 2Gy照射后慢 ,到 180min时 ,DNA损伤残留率明显高于γ射线 2Gy照射。
短句来源
     Separation of residual DNA and proteins were further performed by employing a high saline buffer,and the residual DNA was recovered and cloned. Two DNA fragments named A7 and A23 with typical MARs characteristics were obtained.
     碱性缓冲液分离残留DNA与结合蛋白,回收并克隆残留DNA,测序后得到两个具有MARs序列特征的DNA片段A7和A23。
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  residual dna
As shown by autoradiographic analysis, the residual DNA synthesis does not correspond to 3H-dThd incorporation within a small number of resistant cells, but is located in the nuclei of a high proportion of cells with reduced density of silver grains.
      
Although the numbers of CPDs decreased during darkness, both size fractions showed some residual DNA damage at the end of the night.
      
Furthermore, unlike DNA synthesis in wild-type extracts, residual DNA synthesis in dnaK and dnaJ extracts is thermosensitive.
      
The amount and type of residual DNA synthesis was determined in eight temperature-sensitive mutants of the smut fungusUstilago maydis after incubation at the restrictive temperature (32° C) for eight hours.
      
Following the alignment of chromosomal DNA by amino acid starvation at 33° C no residual DNA synthesis at 42°C takes place (Fig.
      
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A phenylethyl alcohol resistant temperature sensitive DNA replication mutant FD105 was isolated following diethylsulfate mutagenesis.From the observation on the effect of temperature and amino acids starvation on the residual DNA synthesis and the replication of phage X at nonpermissive temperature it was inferred that FD105 is a DNA chain elongation mutant.Temperature sensitiveness and PEA-resistance were proved to be the manifestation of the same mutation,which was localized within the gene...

A phenylethyl alcohol resistant temperature sensitive DNA replication mutant FD105 was isolated following diethylsulfate mutagenesis.From the observation on the effect of temperature and amino acids starvation on the residual DNA synthesis and the replication of phage X at nonpermissive temperature it was inferred that FD105 is a DNA chain elongation mutant.Temperature sensitiveness and PEA-resistance were proved to be the manifestation of the same mutation,which was localized within the gene dnaB.The target of action of PEA is the cell membrane.The fact that the chain elongation mutant dnaB is also a PEA-resistant mutant speaks for the notion that the cell membrane is relevant to DNA chain elongation.

通过硫酸二乙酯诱变,选得一株抗苯乙醇的DNA复制突变型FD105。从温度和氨基酸饥饿对DNA残余合成的影响,以及在限制温度中噬菌体增殖情况可以看到,FD105是DNA复制链延长突变型。我们证实了温度敏感和苯乙醇抗性是同一基因突变的结果。这一基因被定位在dnaB座位。已有的研究表明苯乙醇的作用部位是细胞膜,链延长dnaB突变型同时抗苯乙醇,这是链延长与膜有关的一个旁证。

A preliminary study on application of Fluorometric analy- sis of DNA unwinding(FADU)in the detection of DNA strand breaks indu- ced by indirect environmental mutag- en benzo(a) pyrene[BaP]was condu- cted.The results showed that BaP, activated by PCB-induced rat liver S(?) mixture,could result in obvious inc- rease of DNA strand breaks in human peripheral leukocytes incubated with BaP(0.01,0.1,1.0,10.0,and 100.0μM) for 60 min under 37℃ condition;co- mpared to DMSO solvent control,the residual...

A preliminary study on application of Fluorometric analy- sis of DNA unwinding(FADU)in the detection of DNA strand breaks indu- ced by indirect environmental mutag- en benzo(a) pyrene[BaP]was condu- cted.The results showed that BaP, activated by PCB-induced rat liver S(?) mixture,could result in obvious inc- rease of DNA strand breaks in human peripheral leukocytes incubated with BaP(0.01,0.1,1.0,10.0,and 100.0μM) for 60 min under 37℃ condition;co- mpared to DMSO solvent control,the residual DNA double strands in leuk- ocytes were significantly decreased (P<0.05)and existed a clear dose re- sponse relationship,but these chang- es were not present when inactivated BaP(10.0 and 100.0μM)were used.The results suggested that the effect of DNA strand breaks induced by BaP was caused by the latter's active metabolites;and,FADU is also suita- ble for the detection of DNA strand breaks caused by indirect mutagens and carcinogens.

采用荧光快速检测 DNA 解螺旋(FADU)方法检测了环境致癌物苯并(a)芘在体外对人外周血白细胞 DNA 的损伤作用。结果显示,经大鼠肝 S_■混合液活化的苯并(a)芘在0.01~100.0μM 剂量范围内可使白细胞 DNA 链断裂明显增加,细胞内剩余双链 DNA 降低24~61%(P<0.05),且存在明显的剂量效应关系。未经活化的苯并(a)芘则未出现明显的 DNA 损伤效应。结果还提示,苯并(a)芘的 DNA 损伤效应是由其活性代谢产物引起的,该 FADU 检测方法同(?)适用于检测能引起 DNA 链断裂的间接致突变物和致癌物。

A recombinant E. coli strain expressing (PBC220/IL-2) was used for fermentation and subsequent purification. The purity of final product was over 95%. A technique of the molecular hybridization was applied to detect residual DNA in purified IL-2, using total DNA of E. coli PBV220 cells labelled with ~(32)P as a probe. The result showed that there no DNA could be found in the purified products.

将携带有人白细胞介素-2基因的工程菌(PBV~(220)/ZL-2),发酵培养后经一系列提纯可获得基因工程人白细胞介素-2(rIL-2)的纯品。采用分子杂交技术,用~(32)P标记PBV_(220)大肠杆菌全DNA为探针,检测rlL-2纯品,没有检测出残余DNA,完全符合WHO和“中国人用重组DNA制品质量控制要点”所规定的残余DNA含量在100Pg/剂以下的要求。

 
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