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human fibrinogen
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  人纤维蛋白原
     Determination of arginine in human fibrinogen by HPLC with precolumn derivatization
     柱前衍生化HPLC法测定人纤维蛋白原中精氨酸的含量
短句来源
     Validation of Virus Inactivation in Human Fibrinogen with S/D Treatment
     S/D处理人纤维蛋白原的病毒灭活验证
短句来源
     Virus inactivation with S/D treatment was incorporated into the preparation scheme of human fibrinogen. The final concentration of TNBP and Tween80 in the solution was 0.3% and 1% respectively. The 6 hour treatment at 25℃ could sterilize the marker viruses effectively e.g VSV (>3.75Log),Sindbis(>4.46Log),HIV(>3.67Log).
     在人纤维蛋白原制备工艺中增加S/D处理灭活病毒步骤,TNBP和Tween80终浓度分别为0.3%和1%,在25℃处理6小时能有效灭活指示病毒VSV(〉3.75Log)、Sindbis(〉4.46Log)、HIV(〉3.67Log),盲传三代未检出病毒
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     Methods By using 125 I labeled human fibrinogen (Fg), the Fg specifically bound to human platelets induced by HI117 and SJ9A4 was measured,and the changes of anti CD 9 McAbs exposed Fg receptor in the absence of Fg were also observed.
     方法 利用125 I标记人纤维蛋白原( Fg) ,观察 H I117 和 S J9 A4作用下血小板 Fg 特异性结合量,反映血小板膜上 Fg 受体的暴露情况,并观察无 Fg 存在条件下,由 H I117 和 S J9 A4 诱导的 Fg 受体暴露后的变化。
短句来源
     In the control group Ⅱ 20 mL saline containing human fibrinogen was perfused.
     对照Ⅱ组注入20mL已加入人纤维蛋白原20mg的生理盐水。
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  人血浆纤维蛋白原
     Hydroxyapatite films on adsorptive properties of human fibrinogen
     羟基磷灰石薄膜对人血浆纤维蛋白原的吸附性能
短句来源
     Objective:To explore the fabrication process of hydroxyapatite (HA) films and its adsorptive properties of human fibrinogen (Fib).
     目的:探讨羟基磷灰石(HA)薄膜的制备方法及其对人血浆纤维蛋白原(Fib)的吸附性能。
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  “human fibrinogen”译为未确定词的双语例句
     Methods The jelly-like cartilaginous tissue was constructed by mixing epiphyseal plate chondrocyte, 0. 3% collagen I , 1 000 U · mL-1 thrombin and 20 g · L~1 cryodesiccant human fibrinogen.
     方法:分离获得骺板软骨细胞,加入 0.3%I型胶原酸性溶液、1 000 U·mL-1凝血酶溶液及20 g·L-1人血冻干纤维蛋白原混匀,成为胶冻样软骨组织培养物。
短句来源
     ① Model establishment In the test group 10 mL autologous venous blood collected from the dogs was added into the disinfected plat in which 20 mg human fibrinogen was added in advance and 200 u thrombase was also added and then 5 mm × 5 mm × 5 mm coagulums were sectioned after 2-hour quiet displacement for later use.
     ①模型制备:实验组采犬自体静脉血10mL加入事先已加入人纤维原20mg的消毒平皿内,并加入凝血酶200u,静置2h后切成5mm×5mm×5mm的凝血块,备用。
短句来源
     (1) Preparation of human fibrinogen concentrate : The fibrinogen-rich cohn fraction I was solubilized in a tris/citrate/lysine buffer, pH6.8, and concentrated to 10-20 protein g/l. The solution was clarified using a 1um filter, and treated with a mixture of 0. 3% and 1% Tween 80 at 25C for 8h.
     (1) 纤维蛋白原:采用pH6.8的tris/枸橼酸盐/赖氨酸缓冲液溶解富集纤维蛋白原的cohn组分Ⅰ沉淀糊,溶液经1μm微孔膜浓缩至蛋白浓度为10-20g/1,浓缩液以0.3%TNBP和1%Tween 80在25℃处理8小时。
短句来源
     In the three fibrinogenic reagents: 0. 2% bovine fibrinogen, 0.2% human fibrinogen and sheep plasma, 0.2% bovine fibrinogen had the best clotting sensitivity.
     0.2%牛纤原、0.2%人纤原和绵羊血浆三种底物以0.2%牛纤原敏感性最好。
短句来源
     Objective:To investigate the profile of procagulant activity(PCA) of peripheral blood monocyte(PBMC)in patients with acute on chronic hepatitis and the expression of a novel procoagulant factor,human fibrinogen like protein 2(hfgl2) and its correlation with disease progression.
     目的:了解各种临床类型乙型肝炎患者外周血细胞的促凝血活性(PCA)及新型促凝血因子人纤维介素(human fibrinogen like protein 2,hfgl2)的表达情况,及其与疾病进展的关系。
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  human fibrinogen
Antibody responses generated by mice to the dengue-2 virus NS1 protein (D-2V NS1) were influenced by MHC class II (I-A) haplotype but each antiserum cross-reacted with human fibrinogen, thrombocytes and endothelial cells.
      
Heparin binding sites are located in a 40-kD γ-chain and a 36-kD β-chain fragment isolated from human fibrinogen
      
Limited reduction of a mixture of purified human fibrinogen and human serum albumin leads to the formation of a stable complex.
      
In vitro preparation and partial characterization of a novel complex of human fibrinogen with albumin
      
The adsorption of two proteins from human plasma (human serum albumin (HSA) and human fibrinogen (HFG) onto six different polymeric surfaces (two of which are heparinized), has been studied byin situ ATR/FTIR spectroscopy.
      
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By means of SDS (sodium dodecyl sulfate)-polyacrylamide gel electrophoresis it was demonstrated that the fibrinolytic enzyme of Agkistrodon halys(Pallas) venom not only degrades the a chain but also degrades the chain of human fibrinogcn. A series of the fragments with different molecular weights, X', Y', D', E', could be observed electrophoretically on acid polyacrylamidc gels after degradjng fibrinogen. The mechanism of the hydrolytic action of human fibrinogen by the fibrinolytic enzyme has been discussed....

By means of SDS (sodium dodecyl sulfate)-polyacrylamide gel electrophoresis it was demonstrated that the fibrinolytic enzyme of Agkistrodon halys(Pallas) venom not only degrades the a chain but also degrades the chain of human fibrinogcn. A series of the fragments with different molecular weights, X', Y', D', E', could be observed electrophoretically on acid polyacrylamidc gels after degradjng fibrinogen. The mechanism of the hydrolytic action of human fibrinogen by the fibrinolytic enzyme has been discussed.

应用SDS—聚丙烯酰胺凝胶电泳的方法证明蝮蛇毒纤溶酶对人体纤维蛋白原的α(A)链和β(B)链都有作用。借助酸性聚丙烯酰胺凝胶电泳观察到人纤维蛋白原经蝮蛇毒纤溶酶水解后,产生一系列分子量大小不等的碎片X′、Y′、D′和E′。

Measurement of FDP in menstrual blood could serve as a parameter for the fibrinolyticactivity in the uterine cavity during menstruation. It could be used to determine the relat-ionship between menorrhagia and fibrinolytic activity indirectly. In this paper, the pre-paration of a specific antiserum of rabbit-anti-human fibrinogen was illustrated, and thenormal value of FDP in the menstrual blood from 43 normal adult fertile women was deter-mined by double immunodiffusion. The results showed that the positive...

Measurement of FDP in menstrual blood could serve as a parameter for the fibrinolyticactivity in the uterine cavity during menstruation. It could be used to determine the relat-ionship between menorrhagia and fibrinolytic activity indirectly. In this paper, the pre-paration of a specific antiserum of rabbit-anti-human fibrinogen was illustrated, and thenormal value of FDP in the menstrual blood from 43 normal adult fertile women was deter-mined by double immunodiffusion. The results showed that the positive precipitation linescould still be seen when the dilutions of most specimens of menstral blood supernatants were1:32 or 1:64 and the median was 1:32, corresponding to the concentration of 144 mg/100ml.

测量经血中纤维蛋白(原)降解产物(FDP)的含量能作为反映行经时子宫腔内纤溶活力的一种指标,从而间接地确定月经过多与纤溶活力之间的关系。本文报道了兔抗人纤维蛋白原特异性抗血清的制备及以免疫双扩散法测定了43名正常成年妇女经血中FDP含量,多数正常妇女经血上清液在稀释度为1:32或1:64时仍有阳性沉淀性可见,其中倍数是1:32相当于144毫克/100毫升的浓度。

Five stable hybridoma cell lines secreting desired monoclonal antibodies (McAbs) were prepared by fusing myeloma cells (NSl ) with spleen cells of the BALB/C mice immunized with human fibrinogen. The specificity of the five McAbs(DNl7, DN18, DN19, DN 20, DN42) against fibrinogen was identified and confirmed by double immunodiffusion, immunoelectrophoresis, dot immunodetection, Western blotting and ELISA. Among those McAbs, both DN17 and DN18 reacted with fragment D but their epitope specificity was...

Five stable hybridoma cell lines secreting desired monoclonal antibodies (McAbs) were prepared by fusing myeloma cells (NSl ) with spleen cells of the BALB/C mice immunized with human fibrinogen. The specificity of the five McAbs(DNl7, DN18, DN19, DN 20, DN42) against fibrinogen was identified and confirmed by double immunodiffusion, immunoelectrophoresis, dot immunodetection, Western blotting and ELISA. Among those McAbs, both DN17 and DN18 reacted with fragment D but their epitope specificity was different, the other one, DN20 reacted with fragment E. All of the five McAbs were of IgG_(?).

本文报道用人纤维蛋白原免疫BALB/C小鼠,取免疫脾细胞与NS_1骨髓瘤细胞融合,获得5株稳定分泌抗人纤维蛋白原单克隆抗体(McAb)的杂交瘤细胞株。经双向免疫扩散、免疫电泳、斑点免疫测定、蛋白印迹法及ELISA对单抗表位特异性的分析。确定了DN_(17)、DN_(18)、DN_(19)、DN_(20)和DN_(42) 5个单抗具有抗人纤维蛋白原的特异性,其中DN_(17)和DN_(18)有抗碎片D的特异性,但两者更精细的表位特异性不同,DN_(20)有抗碎片E的特异性,5个单抗均为IgG_1。

 
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