Primers were designed according to the sequences of AtCAL, BoCAL, BobCAL and BoiCAL gene, and PCR amplifications were performed with the genomic DNA of Brussel Sprouts, Kale and Kohlrabi respectively, PCR fragments of about 1.6kb were obtained and designated correspondingly as BogCAL5', BoaCAL5' and BocCAL5'.
Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding PCR product from Brussel Sprouts, Kohlrabi and Kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon.
The results showed that the treatments of 1.0～1.5 g/L B_9 are effective in reducing the height of the potted Kale, increasing the thickness of leaves, reducing the area of leaf, increasing the content of chlorophyll, darkening the leaf color and delaying coloring, and effectively improve the appreciation value of the potted Kale.
In an experiment, the effects of N and K fertilizer on the plant growth ,yield and guality of flower stalk in the Chinese kale cv. Denfeng were studied by means of six fertilizer treatments, namely, N1,K1,N1K1,N1K2,N2K1, and N2K2. The results showed that in the Kitreatment.
The development, survivorship, reproduction, host-feeling and population trend index of E. formosa on Chinese kale were better than sweetpotato and tomato, which were 97%, 23.6±1.6, 162.4±4.3, 69.3±3.4 and 197.2 respectively.
Effect of different extract methods, lysing solutions, and the incubation time of QR reaction mixtures on Quinone reductase bioassay was studied, and the results showed that the QR inducer potency of Chinese Kale phosphate buffer (5 mM K2HPO4-KH2PO4, 1 mM EDTA, pH 7.6) extracts was comparable to the potency of common triple solvent extracts.