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family gene
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  家族基因
     Analysis of the ABC Transporter Family Gene Homologous Sequence ctg16 of Phanerochaete Chrysosporium
     黄孢原毛平革菌(Phanerochaete chrysosporium)ABC转运蛋白家族基因同源ctg16序列的分析
短句来源
     Analysis of PFL family gene in Escherichia coli K-1 2 from bioinformatics
     大肠杆菌K-12中PFL家族基因的生物信息学分析
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     Grid Trust Model Based on Family Gene
     基于家族基因的网格信任模型
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     Expression of interferon alpha family gene of Chinese marmot in eukaryotic and prokaryotic cells
     中国旱獭干扰素α家族基因在真核细胞和原核细胞中的表达
短句来源
     The Role of IL-1 Family Gene Polymorphisms in Sepsis
     细胞因子IL-1家族基因多态性与全身感染
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  “family gene”译为未确定词的双语例句
     Molecular Cloning and Sequence Analysis of DEAD-box Family Gene PL10 from Monopterus albus
     黄鳝DEAD-box家族PL10基因的克隆与序列分析
短句来源
     Expression Profiling of Malignant Melanoma and Validation of Caspase, IAP, Bcl-2 and Cytokeratin Family Gene Expression
     黑色素瘤基因表达谱和Caspase家族、IAP家族、Bcl-2家族及Cytokeratin家族分子改变的研究
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     Objective To investigate the function of interferon alpha(IFN-α) in the woodchuck (Mar-mota monax) model of hepatitis B, the woodchuck IFN-αfamily gene(IFNA) was cloned, and analysis cellular immune status of woodchuck with chronic woodchuck hepatitis virus(WHV) infection.
     目的克隆土拨鼠α干扰素(IFN-α)新亚型基因,用于土拨鼠HBV模型探索IFN-α治疗慢性乙型肝炎策略; 调查慢性土拨鼠肝炎病毒(woodchuck hepatitis virus,WHV)感染的土拨鼠外周血单个核细胞(PBMC)干扰素功能状况。
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     By both analysis of nucleotide and deduced amino acid sequence similarity, T12C-P1 and T12A-AP4 had significant identity to atpH gene encoding ATPase subunit III and sodium channel family gene respectively which had been previously identified as salt-induced expressed gene;
     对其进行测序,序列相似性分析表明T_(12)C-P1片段与atpH基因(该基因编码ATPase的一个亚基)有很高同源性,为重要的抗盐碱基因;
短句来源
     It also had PI motif in the C box region,which was conserved sequence in GLO family gene. The expression of LLGLO1 in different lily tissues was detected by RT-PCR.
     通过RT-PCR检测,百合不同组织中的LLGLO1基因表达模式与郁金香的GLO类基因相似。
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  相似匹配句对
     Family
     家庭
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     family
     家庭·亲情
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     The FXR Gene Family
     FXR基因家族
短句来源
     Paraoxonase Gene Family
     对氧磷酶基因家族
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  family gene
The glutamate-induced apoptosis appears to involve the modulation of Bcl-2 family gene products such as Bcl-2, Bcl-xL, and Bax-α.
      
Thus, these results uncover the molecular mechanism by which NF-κB signalling contributes to the regulation of gadd family gene expression induced by arsenic.
      
While classic approaches to cytoprotection based on Bcl-2 family gene delivery have significant limitations, cellular protein transduction represents a new and exciting approach utilizing peptides and proteins as drugs with intracellular targets.
      
A less frequent variant translocation t(1;13) involves another PAX family gene, /PAX7/, located in chromosome 1 and /FOXO1/ and is present in 10-15% of cases of the alveolar subtype in RMS.
      
Finally, we identified an ERF family gene that is cooperatively regulated by both hormones, and designated as cooperatively regulated by ethylene andjasmonate 1 (CEJ1).
      
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High molecular weight genomic total DNA was extracted from the surgical samples ofhuman lung squamous cell carcinoma in patients with the habit of smoking. All were nottreated by chemotherapy. The two-round transfection tests were carried out with NIH/3T3cell system.The second round transformation was 3.0 times as efficient as that of the firstone. It seemed that the number of transforming focus increased with the amount of DNAused. The second round transforming cells were cultured alive in soft agar and implanted...

High molecular weight genomic total DNA was extracted from the surgical samples ofhuman lung squamous cell carcinoma in patients with the habit of smoking. All were nottreated by chemotherapy. The two-round transfection tests were carried out with NIH/3T3cell system.The second round transformation was 3.0 times as efficient as that of the firstone. It seemed that the number of transforming focus increased with the amount of DNAused. The second round transforming cells were cultured alive in soft agar and implanted intonude mice(BALB/c). The tumor grew under the subcutaneous at injected site. It was showedthat the cells possessed the character of tumor cell. The DNA of the primary and secondarytransformed cells was analysed by Southern Blot with 32P labeled human Alu DNA sequenceand ras family gene as probes. The findings indicated that the positive bands hybridizingwith human Alu sequence were present in DNA extracted from the both transformed and thetumor cells from nude mice. It was identified that human lung carcinoma genomic DNA wasintegrated into these cells. And Ha-ras gene was considered as the putative transforming se-quence in relation with smoking.

从有长期吸烟嗜好的肺癌患者的手术标本(鳞状上皮癌)提取高分子量的癌细胞基因组DNA,对小鼠成纤维细胞(NIH/3T3)进行转染,获二轮转化细胞,发现二轮转化率是一轮的3倍.证实在转染过程中转化灶出现的多少,与所用DNA的量有关,二轮转化细胞可在软琼脂上培养存活,接种裸鼠(BALB/c)于注射部位长出肿瘤。表明该二轮转化细胞具有肿瘤细胞的特性,用32P标记的人Alu重复序列和ras癌基因家族探针分别与一轮、二轮转化细胞和裸鼠肿瘤细胞的DNA进行Southern印迹转移和分子杂交。结果在三者细胞的DNA中都见有与Alu杂交的阳性条带,从而证明在转染过程中人体特有的Alu重复序列已整合到转化细胞的基因组中,并确定了转化细胞中的转化基因之一的属性为Ha-ras癌基因,本工作提示吸烟可能是人体原癌基因(C-Ha-ras)活化和肺鳞癌发生的重要原因。

To amplify and clone the gene fragment carrying the extracellular ligand binding region of erk, an overexpressing EPH family gene in human gastric cancer. Methods: Total RNA was extracted from a surgical specimen of human gastric cancer and subjected to reverse transcription to cDNA. Primers for RT-PCR were designed by computer analysis and chemically synthesized. The amplified fragment was cloned into pUC19 vector and the recombinant clones were confirmed by PCR and restriction endonuclease cleavages....

To amplify and clone the gene fragment carrying the extracellular ligand binding region of erk, an overexpressing EPH family gene in human gastric cancer. Methods: Total RNA was extracted from a surgical specimen of human gastric cancer and subjected to reverse transcription to cDNA. Primers for RT-PCR were designed by computer analysis and chemically synthesized. The amplified fragment was cloned into pUC19 vector and the recombinant clones were confirmed by PCR and restriction endonuclease cleavages. Results: The PCR products was specific and identical to the reported sequence. It was revealed that the primers were correctly designed. Nested-PCR, PCR-RFLP and restriction endonuclease analysis proved that amplified fragment had been inserted in the BamHI and HindIII site of pUC19, and one of the desired clone was named pGE42. Conclusion: The results will offer the basis for further investigation of erk gene including its biological functions involved in carcinogenesis in the stomach, identification of extracellular ligands and in situ localization of its gene product.

对在胃癌高表达的EPH家族基因erk胞外的配体结合区基因扩增及克隆.方法:从胃癌患者手术切除标本中提取RNA,反转录为cDNA,以计算机辅助分析设计并合成引物进行RT-PCR,并将扩增片段克隆入pUC19质粒,用酶切及PCR方法筛选鉴定阳性克隆.结果:RT-PCR扩增片段与设计相符,且特异性好,无非特异产物出现,表明所设计引物符合要求.酶切及巢式PCR、PCR-RFLP方法证明克隆片段正确插入pUC19的HindIII和BamHI位点,并将重组克隆命名为pGE42.结论;克隆的完成为进一步研究该基因在胃癌的发生中的作用,寻找其胞外配体及进行表达产物的细胞定位等工作打下基础.

Objective To evaluate the relationship of p53 alteration and myc family gene overexpression with the clinical characteristics of lung cancer. Methods A series of 59 resected primary lung cancer specimens was analyzed for p53 gene by DNA/PCR sequencing and immunohistochemistry technique, and for myc family genes by RT PCR methods. Results Thirty seven of 57 tumors were found to have p53 mutations or/and p53 protein accumulation. The presence of p53 alteration was not related...

Objective To evaluate the relationship of p53 alteration and myc family gene overexpression with the clinical characteristics of lung cancer. Methods A series of 59 resected primary lung cancer specimens was analyzed for p53 gene by DNA/PCR sequencing and immunohistochemistry technique, and for myc family genes by RT PCR methods. Results Thirty seven of 57 tumors were found to have p53 mutations or/and p53 protein accumulation. The presence of p53 alteration was not related to tumor size, lymph node metastasis, stage and relapse. Forty seven cases were analyzed for myc family genes. The results showed that there was a positive correlation between unregulative expression of myc genes and the above mentioned clinical parameters. Our finding also showed that 19 of 30 cases (63%) with p53 alteration had myc gene overexpression which occurred in 63% and 76% cases with stage Ⅲ and relapse, respectively, which was higher than 27% and 22% with p53 alteration but no myc gene overexpression and 50% and 71% with p53 negative but myc gene overexpression. Conclusions p53 alteration is a vital genetic event in the earlier stage of lung carcinogenisis, but not a prognostic marker. myc family genes overexpression may be regarded as one of the independant prognostic determinants in lung cancer. The cooperation between p53 alteration and myc gene overexpression may occur during progression of lung cancer, but prognostic determinant is myc gene overexpression.

Relationshipofp53alterationandmycfamilygeneoverexpresionwiththeclinicalcharacteristicsoflungcancerBaiLi白莉,SunYan孙燕andLiShende...

 
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