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chromosome counting
相关语句
  染色体计数
     Some intergeneric hybrids (Sugarcane × Erianthus fulvus ) were identified by chromosome counting and RAPD (Random Amplified Polymorphism DNA) technique.
     采用体细胞染色体计数和RAPD(Random Amplified Polymorphism DNA)分子标记的方法鉴定甘蔗与蔗茅属间杂交种F1的真实性。
短句来源
     Chromosome Counting in Root--tip Cells of Reed
     芦苇根尖细胞的染色体计数
短句来源
     Chromosome counting of root tip of the regenerated plantlets showed that the cells with 2n=12 made up a certain proportion and the plantlets were haploids derived from pollen.
     再生植株的根尖细胞的染色体计数表明,约有1/4的细胞2n=12,证明再生植株是来源于花粉细胞的单倍体植株。
短句来源
     The results indicated that chromosome counting was the most reliable and precise method, however, it was cumbersome and required skilled cytological techniques;
     结果表明 :体细胞染色体计数法是最可靠的直接方法 ;
短句来源
     Chromosome counting of microspores at first mitotic metaphase in barley-tetraploid wheat hybrids showed that more than 60 percent of the microspores had 21 chromosomes and were recognized as unreduced male gametes.
     大麦×四倍体小麦杂种小孢子的染色体计数表明,杂种中未减数雄配子的频率在60%以上。
短句来源
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  “chromosome counting”译为未确定词的双语例句
     Results showed that the 1/2MS(1/2N)medium contained 24-D 1.0 mg/L+ZT 1.1 mg/L+CH 1000 mg/L was better for inducing callus to from regenerated plantlets, Stomatal characteristics、flow cytometric analysis and chromosome counting proved dodecaploid(2n=12x=180) presence in those regenerated plantlets.
     结果表明 :以 1/ 2MS (1/ 2N ) +ZT (玉米素 ) 1 1mg/L +2 4 D 1 0mg/L +CH (水解酪蛋白 ) 10 0 0mg/L诱导产生的愈伤组织易获得再生植株 ; 形态学和细胞学检测确认再生植株中存在 2n =12x =180的个体。
短句来源
     Translocation heterozygotes resistant to BYDV were selected of the backcrossing progenies after enzyme linked imunosorbent assay (ELISA) , analysis of restriction fragment length polymorphism(RFLP),root tip chromosome counting and field resistant test. Some promising lines with consistant BYDV resistance, such as D1091, D1094, D1658,TC 5,TC6,TC7 were developed.
     经酶联免疫吸附分析(ELISA)、限制性内切酶长度多态性分析(RFLP)、染色体数目的检查和田间抗性鉴定,在再生植株回交后代中获得了抗BYDV的杂合易位株,经自交和花药培养纯合,选育出D1091、D1094、D1658、TC5、TC6、TC7等一批抗性稳定或基本稳定的普通小麦选系。
短句来源
     Root_tip chromosome counting of more than 20 randomly selected plants revealed that they were all tetraploids (2n=4x=36).
     随机检查 2 0余株再生苗根尖染色体数目 ,表明都为四倍体 (2n =4x =36 )。
短句来源
     Triploid rates of gastrula(by chromosome counting),1d larvae and 120d juveniles(by flow-cytometry) were 34.03%,29.54% and 14.81%,respectively,which indicated that the triploid rates of induction groups decreased with development and growth of P.crocea.
     (3)采用冷休克法进行大黄鱼三倍体大批量诱导,早期胚胎、初孵仔鱼和4月龄幼鱼三倍体检出率分别为34.03%、29.54%和14.81%,表明随生长发育诱导组三倍体检出率有下降趋势。
短句来源
     Three clones (No.1~No.3) were regenerated from the fusion products and differentiated into albino seedlings. The clones and the seedlings were all verified as hybrids by chromosome counting, isozyme and RAPD analysis.
     融合再生的3个单细胞克隆(No.1~No.3)经染色体、同工酶和RAPD分析表明,它们均为杂种细胞系。
短句来源
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  相似匹配句对
     Chromosome Counting in Root--tip Cells of Reed
     芦苇根尖细胞的染色体计数
短句来源
     The counting of the chromosome number and the karyotype of Citrus grandis (L.)
     本文首次对垫江柚(Citrus grandis(L.)
短句来源
     8 chromosome.
     8染色体
短句来源
     2 of chromosome.
     RET基因定位于 10号染色体长臂 1区 1带 2亚带(10all.2)。
短句来源
     Counting Chickens
     数鸡
短句来源
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  chromosome counting
We demonstrate that both genome size and the number of nucleoli may be used to determine the ploidy level as an alternative to chromosome counting.
      
Distribution maps based on plants investigated either by chromosome counting or by flow cytometry, augmented by similar data from published sources are given for both subspecies in Europe.
      
Koch sampled in the Czech Republic, Germany, Austria and Poland was determined by chromosome counting and/or by flow cytometry.
      
The experiment demonstrated a positive correlation between the ploidy estimated by chromosome counting and DNA flow cytometry inS.
      
In addition to chromosome counting, flow cytometry was employed to screen ploidy levels.
      
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The development of haploid plants has been successfully induced from wheat (Triticum vulgare)pollens by means of the anther cultured in vitro.On the MS medium supplemented with 2-20mg/l 2,4-D and various organic addenda,out of 21094 inoculated anthers taken for culture from 27 hybrids or varieties,103 produced calli.All the calli developed from dehisced anther sacs.Microscopical examinations indicated that these calli were formed by repeated divisions of the pollen grains.5 days after,the pollen calli were transferred...

The development of haploid plants has been successfully induced from wheat (Triticum vulgare)pollens by means of the anther cultured in vitro.On the MS medium supplemented with 2-20mg/l 2,4-D and various organic addenda,out of 21094 inoculated anthers taken for culture from 27 hybrids or varieties,103 produced calli.All the calli developed from dehisced anther sacs.Microscopical examinations indicated that these calli were formed by repeated divisions of the pollen grains.5 days after,the pollen calli were transferred into the medium containing either 0.2-2mg/l NAA and 0.2-2mg/l kinetin or 0.5mg/l IAA and 15% cocoanut milk,differentiation of shoots began to occur.It was also found that if the anthers were inoculated on the MS medium containing either 20% cocoanut milk or 2mg/l IAA and 2mg/l kinetin,shoots would develop directly from the anther sacs.The chromosome counts in root tips of 6 plantlets examined gave the number 21,confir- ming that they are haploids.The haploid plants produced spikes but did not set seeds.

采用离体培养小麦(Triticum vulgare)花药的方法成功地诱导小麦花粉形成了单倍体植株。在附加2—20毫克/升的2,4-D 和各种有机附加物的 MS 培养基上,属于27个杂种或品种的21094个花药中有103个花药产生了愈伤组织,这些愈伤组织均着生在裂开的药室内,显微观察证明它们是由花粉经过多次细胞分裂形成的。花粉愈伤组织转移到含有0.2—2毫克/升的萘乙酸和0.2—2毫克/升的激动素或含有0.5毫克/升的吲(口朶)乙酸和15%椰乳的 MS培养基上培养5天以后,即陆续分化出幼苗。此外还发现接种在含有20%椰乳或吲(口朶)乙酸及激动素各2毫克/升的 MS 培养基上的花药直接从药室内产生出幼苗。已检查的6株幼苗的根尖细胞的染色体数均为21,表明它们是单倍体。单倍体植株能够抽穗而不结实。

A human diploid cell strain derived from lung tissue of a four month old female foetus and designated KMB-13 has been established. The cell morphology of KMB-13 was fibroblast. In appearance it was transparent, highly refractile and regularly arranged. The cell cultures grew rapidly and could be carried out to subcultivate readily in vitro. The original passage series of the strain, which was cultivated serially without frozen and stored, attained 48 passages at a 2 : 1 split ratio. Because of an average of...

A human diploid cell strain derived from lung tissue of a four month old female foetus and designated KMB-13 has been established. The cell morphology of KMB-13 was fibroblast. In appearance it was transparent, highly refractile and regularly arranged. The cell cultures grew rapidly and could be carried out to subcultivate readily in vitro. The original passage series of the strain, which was cultivated serially without frozen and stored, attained 48 passages at a 2 : 1 split ratio. Because of an average of 1.102 cell population doublings per passage, the total lifetime of KMB-13 was approximately 53 doublings. For karyological investigation, cells taken from cultures at 5, 12, 28, 36, and 42 passages were examined for exact chromosome counts and for analysis of karyotype. The counts of 2n number of 46 were 81.8 per cent, and the karyotypes for 40 cells at various passage levels were 46/XX. Chromosome analyses have shown the human cell strain to be typical diploid appearance.The cells of partial cultures after the eighteenth passage were harvested in ampules and preserved successively in liquid nitrogen as a frozen stock. After 8 months, the ampules were thawed to reconstitute and had a viability of cells of 85.9 per cent. Later studies have shown that the cells reconstituted from the frozen state did not alter in vitro life span and other biological characteristics. Preliminary studies made to detect the extraneous agents were negative. KMB-13 cells had a relatively broad spectrum of human viral susceptibility.

本文介绍了建立人二倍体细胞株的初步结果,并对其应用范围作了简要讨论。 我们从一个四月龄的女性胎儿肺组织建立了一株人二倍体细胞株,名为KMB-13。细胞株原系以1:2分种率传代48次终止,平均每代为1.102次细胞群体分裂,整个寿命约合53次细胞群体分裂。18代以后细胞于液氮中陆续冻存。传代过程染色体分析始终保持二倍体性。初步检查未发现潜在病毒因子。对人类病毒有较广泛的敏感性。

It was successful to induce pollen plants from the in vitro anthers of 56 chromosome hybrid of Triticum vulgare×Agropyron glaucum cultured on the MS medium supplemented with auxin, coconut milk and mononucleotide. The pollen plants such obtained were mostly albinos, partly green and white mosaic and only three of them were normally green. Chromosome counts made in the root tip cells revealed that most pollen plants were haploids (2N = 28), however, one of them possessing 27 chromosomes only...

It was successful to induce pollen plants from the in vitro anthers of 56 chromosome hybrid of Triticum vulgare×Agropyron glaucum cultured on the MS medium supplemented with auxin, coconut milk and mononucleotide. The pollen plants such obtained were mostly albinos, partly green and white mosaic and only three of them were normally green. Chromosome counts made in the root tip cells revealed that most pollen plants were haploids (2N = 28), however, one of them possessing 27 chromosomes only was monosomici haploid and it showed prominent changes in its morphological characteristics. The chromosome behaviour in the course of meiosis of the sporocytes was found that local homology existed in 1-8 pairs of chromosome. The bivalents were rod-shaped with terminal conjugation, ultrastructural observations on the leaf cells of albino plant demonstrated the existence of the plastids, however, the development of the plastids was limited and could not form chloroplasts with normal lamella structure. The possible origin of the karyotype of the 56 chromosome hybrid was discussed.

离体培养具56个染色体的小麦(Triticum vulgare)和冰草(Agropyron glaucum)杂种的花药,在附加生长素、椰乳和单核苷酸的MS培养基上,成功地诱导出花粉植株。多数植株为白化体,一部分是绿白条纹体,仅有3株是正常的绿色体。检查根尖细胞染色体证明绝大多数植株为单倍体(2n=28),但发现其中1株是单缺体,只有27个染色体,并出现形态特征上的深刻变化。观察花粉母细胞减数分裂时染色体的行为发现,在1—8对染色体间有局部同源性,二价体表现为棒形端部结合。对白化体叶片细胞的亚显微结构研究表明,细胞中存在质体,但质体的发育受到阻碍,不能形成具有正常片层结构的叶绿体。讨论了具56个染色体的杂种其核型的可能形成过程。

 
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