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antiserum
相关语句
  抗血清
    IDENTIFICATION, PURIFICATION AND ANTISERUM PREPARATION OF TOBACCO NECROSIS VIRUS INFECTING POTATOES
    侵染马铃薯的烟草坏死病毒的鉴定、提纯及抗血清制备
短句来源
    IDENTIFICATION OF CARNATION MOTTLE VIRUS AND ITS ANTISERUM PREPARATION
    香石竹脉斑驳病毒(CarVMV)鉴定和抗血清制备
短句来源
    Studies on the Preparations of Antiserum and Serological Detection Techniques for Strawberry Pseudo Mild Yellow Edge Virus
    草莓伪温和黄边病毒抗血清制备及血清学检测技术研究
短句来源
    PURIFICATION, ANTISERUM PREPARATION AND ELISA WITH THE PBM1 (PLUM PSEUDOPOX ) STRAIN OF APPLE CHLOROTIC LEAF SPOT VIRUS (ACLSV)
    苹果褪绿叶斑病毒PBM1(李假痘)毒株的提纯、抗血清制备及ELISA检测
短句来源
    Preparation of the three kinds of antiserum against quarantine viruses and its detection by SPA ELISA
    几种危险性病毒抗血清的制备及检测方法的建立
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  “antiserum”译为未确定词的双语例句
    Cloning and Expression of a Baculovirus J Domain Protein Gene in E. coli and Preparation of Antiserum
    杆状病毒J结构域蛋白基因的克隆、表达和抗体的制备
短句来源
    Molecular Cloning and Expression of the fp25k Gene of HaSNPV in E. coli and Preparation of Its Antiserum
    棉铃虫病毒HaSNPVfp25k基因的克隆表达及抗体制备
短句来源
    The greatest inhibition of the antiserum obtained from SP-BSA were recorded with acephate, dimethoate, parathion-methyl and malathion, the 50% inhibition were at 2.66, 3.31, 8.26 and 14.9 u g/mL, the limit of detection (I20) were 0.05 ,0.15, 0.19 and 0.53 u g/mL, respectively.
    SP产生的抗体对乙酰甲胺磷、乐果、甲基对硫磷和马拉硫磷都表现了一定的交叉反应,四种化合物对SP抗原抗体结合反应产生抑制的I_(50)分别为2.66、3.31、8.26和14.9μg/mL,方法检测限(I_(20))分别为0.05、0.15、0.19和0.53μg/mL。
短句来源
    The GST-ORF5 fusion protein has been expressed in Escherichia coli BL21 (DE3) LysS cells and anti-GST-ORF5 antiserum has been prepared. Characterization of ORF5product needs to be carried out further.
    构建了ORF5与pGEX-4T-2的GST融合表达载体,在大肠杆菌BL21中得到了表达,并制备了兔多克隆抗体。
短句来源
    The antiserum of ZYMV was prepared and provides condition for the identification of ZYMV. The CP gene of ZYMV was amplified by RT-PCR, and ligated to the expression vector of pET-22b(+) . The recombinant plasmid pET-ZCP was transformed into E. coli BL21 (DE3) and then induced by IPTG to express a fusion protein .
    采用RT-PCR方法自扩增出小西葫芦黄花叶病毒(Zucchini yellow mosaic virus, ZYMV)的CP基因,连接到原核表达载体pET-22b(+)上,获得的重组子pET-ZCP转化大肠杆菌BL21(DE3)后,用IPTG进行诱导表达。
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  antiserum
A polyclonal antiserum to the noncatalytic part of cellobiohydrolase I fromTrichoderma reesei
      
Immunization of BALB/c mice by horse antiserum against diphtheria made it possible to obtain IgG1 monoclonal antibodies (MoAbs) 2B7E4 specific for light chains of horse immunoglobulin (Ig).
      
The binding was inhibited by monoclonal antibodies (mAb) to region 46-70 of M1 and by an antiserum to region 21-45, whereas mAb to the middle and C-terminal regions had no effect.
      
Pronounced reactions of major glycolipids and phospholipids with a homologous polyvalent antiserum against B.
      
The antiserum contained high titers of specific antibodies against glycolipids and phospholipids of bifidobacteria, as demonstrated by heterogeneous solid-phase enzyme immunoassay (ELISA).
      
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In order to study the distribution of the virus entities which cause“Kwuting”symptomsof Chinese cabbage on various cruciferous crop plants,diseased specimens were collected fromPeking and Tientsin throughout the growing season in 1962.Isolations and routine diagnosiswere made during 1962-63.On basis of the differential host reactions,109 isolates weregrouped into seven types,among which types Ⅰ,Ⅱ and Ⅲ being reacted with K_1 (TurnipMosaic Virus) antiserum were thus considered to be related strains.These...

In order to study the distribution of the virus entities which cause“Kwuting”symptomsof Chinese cabbage on various cruciferous crop plants,diseased specimens were collected fromPeking and Tientsin throughout the growing season in 1962.Isolations and routine diagnosiswere made during 1962-63.On basis of the differential host reactions,109 isolates weregrouped into seven types,among which types Ⅰ,Ⅱ and Ⅲ being reacted with K_1 (TurnipMosaic Virus) antiserum were thus considered to be related strains.These strains were de-signated K_(1-2) for type Ⅰ,K_(1-3) for type Ⅱ and K_(1-4) for type Ⅲ.Type Ⅰ neither infectedNicotiana tabacum nor N.glutinosa.Type Ⅱ did infect N.tabacum causing local lesions,butnot N.glutinosa.Type Ⅲ with its T.D.P.slightly higher than 65℃ did infect N.glutinosacausing local lesions,but might or not infect N.tabacum.Type Ⅳ being identical to theKwuting Virus 1 (A type strain of Turnip Mosaic Virus on Chinese cabbage) was designatedK_(1-1).Types Ⅴ and Ⅵ had not yet been identified,while type Ⅶ was identical to the former-ly reported virus K_3 (A ringspot strain of TMV).In fields,types Ⅰ and Ⅱ occurred more frequently.They were 59.0 and 32.0 per cent ofthe total isolates respectively.Type Ⅲ was apparently less prevalent (5.5 percent) and theother types occurred more rarely (less than 0.9 percent each).Therefore the two TurnipMosaic Virus strains K_(1-2) and K_(1-3) were the most prevalent and responsible for the epiphyto-tics of the Kwuting disease of Chinese cabbage in 1962.However,in Peking K_(1-2) was lessabundant than K_(1-3),while in Tientsin the condition was quite the reverse.The difference ofstrain dominance might be attributed to the different varieties of Chinese cabbage cultivatedin these two localities.For instance,the main Chinese cabbage variety cultivated in Pekingwas“Qinpaikou”,from which the isolates of K_(1-3) and K_(1-2) were in the ratio of 3:2.Themain Chinese cabbage variety grown in Tientsin was“Tianjinl(?)”,from which the isolates ofK_(1-3) and K_(1-2) was in the ratio 1:5.Isolates made from two kinds of seeding plants,namely the overwintered seed plants plant-ed out in spring and the seed plants grown from vernalized seeds,revealed that they wereessentially K_(1-2) and K_(1-3).It was believed that these virus entities were carried in the over-wintered seed plants and disseminated to other cruiferous plants after planting out in spring.These two essential virus entities were isolated from successive cruciferous crops throughoutthe growing season.Therefore the summer sown cabbage served as one of the principal hostsfor the virus to tide over summer,while the autumn Chinese radish which was sown usually10-15 days earlier than Chinese cabbage,served as one of the important bridge hosts.

1962年至1963年系统地调查北京及天津白菜区十字花科作物上逐月出现的病毒类型及其相对数量,以确定十字花科作物之间病毒的相互关系。从109个分离物中,根据寄主反应,区分成7个类型,其中Ⅰ、Ⅱ、Ⅲ型都和芜菁花叶病毒(白菜孤丁病毒1号即 K_1)的抗血清起反应,因此认为它们是白菜孤丁病毒1号的相关株系:Ⅰ型定为 K_(1-2),根本不侵染菸草;Ⅱ型定为 K_(1-3),能侵染普通菸,产生局部坏死斑,但不侵染心叶菸;Ⅲ型定为 K_(1-4),能侵染心叶菸产生局部坏死斑,能或不能侵染普通菸与产生局部坏死斑,致死温度稍高于65℃;Ⅳ型为典型的白菜孤丁病毒1号的原株系(K_(1-1));Ⅴ及Ⅵ型是未经鉴定的毒原;Ⅶ型是典型菸草环斑型花叶病毒(K_3)。京、津两地109个分离物中,Ⅰ型(K_(1-2))占59%,Ⅱ型(K_(1-3))32%,Ⅲ型(K_(1-4))5.5%,Ⅳ型(K_(1-1))0.9%,Ⅴ型0.87%,Ⅵ型0.87%,Ⅶ型(K_3)0.87%。其中以 K_(1-2)及 K_(1-3)(芜菁花叶病毒亦即白菜孤丁病毒1号)为主,其他类型均不重要。北京及天津两地十字花科作物上病毒类型的分布显然有些差别,在北京...

1962年至1963年系统地调查北京及天津白菜区十字花科作物上逐月出现的病毒类型及其相对数量,以确定十字花科作物之间病毒的相互关系。从109个分离物中,根据寄主反应,区分成7个类型,其中Ⅰ、Ⅱ、Ⅲ型都和芜菁花叶病毒(白菜孤丁病毒1号即 K_1)的抗血清起反应,因此认为它们是白菜孤丁病毒1号的相关株系:Ⅰ型定为 K_(1-2),根本不侵染菸草;Ⅱ型定为 K_(1-3),能侵染普通菸,产生局部坏死斑,但不侵染心叶菸;Ⅲ型定为 K_(1-4),能侵染心叶菸产生局部坏死斑,能或不能侵染普通菸与产生局部坏死斑,致死温度稍高于65℃;Ⅳ型为典型的白菜孤丁病毒1号的原株系(K_(1-1));Ⅴ及Ⅵ型是未经鉴定的毒原;Ⅶ型是典型菸草环斑型花叶病毒(K_3)。京、津两地109个分离物中,Ⅰ型(K_(1-2))占59%,Ⅱ型(K_(1-3))32%,Ⅲ型(K_(1-4))5.5%,Ⅳ型(K_(1-1))0.9%,Ⅴ型0.87%,Ⅵ型0.87%,Ⅶ型(K_3)0.87%。其中以 K_(1-2)及 K_(1-3)(芜菁花叶病毒亦即白菜孤丁病毒1号)为主,其他类型均不重要。北京及天津两地十字花科作物上病毒类型的分布显然有些差别,在北京地区 K_(1-3)占51.2%,K_(1-2)占34.1%,K_(1-4)占7.3%,其余 K_(1-1),Ⅴ及Ⅵ各占2.5%,无 K_3,而在天津地区则 K_(1-2)占68.8%,K_(1-3)占22.9%,K_(1-4)占6.3%,有 K_3的出现(2.1%),而无 K_(1-1),Ⅴ及Ⅵ。这种差异可能与当地所栽品种及十字花科蔬菜种类有关,因为北京的白菜以青白口品种为主,而 K_(1-3)和 K_(1-2)在青白口品种上的出现此数为3∶2。天津地区的白菜品种邓鄯沽大核桃纹、高杆连心壮及天津绿上的 K_(1-3)和 K_(1-2)的出现比数相应为1∶5,1∶2及3∶4。根据两种采种株,即越冬菜株及当年播的春化种子的种菜上的毒原类型分析,一般均为,K_(1-2)及 K_(1-3),而出现的次数在越年采种株上为多,可见越年采种株是白菜孤丁病毒的主要越冬寄主之一,春化种子的种菜上的病原可能是从越冬采种株上传来的,根据3—10月各种十字花科作物上病毒类型出现次数的分析,K_(1-2)及 K_(1-3)在四至五月间在留种菜、小白菜、油青菜及甘兰上达到高峯,六月间则出现在白菜及甘兰上,最后则出现在秋白菜上,其中甘兰是京津六至九月间的连续作物,因此作为主要越夏寄主的可能性更大,而萝卜则因其播种较秋白菜早10天至15天,正当传毒翅蚜的发生期,因此认为是一个比较危险的过渡寄主。

Investigations on the effect of various mono-, di-, and trivalent metallicsalts and fungicidal substances on the free phages of Xanthomons oryzaejndicated that the most prevalent OP1 type phage of the organism was inactivatedboth by ferrous and ferric salts, while ferric salts were more effective thanferrous salts. Iron salts at proper concentrations will inactivate the freephages but without any noticeable effect on those adsorbed and the susceptiblehostbacteria. The concentration of the solution required to...

Investigations on the effect of various mono-, di-, and trivalent metallicsalts and fungicidal substances on the free phages of Xanthomons oryzaejndicated that the most prevalent OP1 type phage of the organism was inactivatedboth by ferrous and ferric salts, while ferric salts were more effective thanferrous salts. Iron salts at proper concentrations will inactivate the freephages but without any noticeable effect on those adsorbed and the susceptiblehostbacteria. The concentration of the solution required to inactivate the phagesvaries between 3-5×10~(-5) M to 1×10~(-3)M depending on the concentration ofthe phage suspension to be inactivated, but a solution of 1×10~(-4)M seems to besuitable in most cases. Results of experiments indicate that the iron salts arevery similar to the antiserum in their reaction to the phages, and can be usedin place of antiserum in various aspects of phage investigations.

在测定单价、二价和三价金属盐和杀菌物质等对水稻白叶枯病菌[Xantho-monas oryzae (Uyeda et Ishiyama)Dowson]噬菌体影响的过程中,发现铁盐可以钝化OP1型的游离噬菌体,而时已经被吸附的噬菌体则完全没有影响。铁盐的钝化噬菌体主要是铁离子的作用。三价铁盐的钝化作用比二价亚铁盐强。对低浓度的噬菌体悬浮液(n×10~4/毫升),其最低有效浓度为3-5×10~(-3)M;对高浓度(n×10~(8-9)/毫升)的噬菌体悬浮液,其最低有效浓度为1×10~(-3)M。铁盐溶液的浓度超过1×10~(-3)M时,不仅使游离噬菌体全部钝化,对细菌也有致死作用。铁盐对噬菌体的作用,与噬菌体抗血清的作用在有些方面是相似的。在噬菌体性状的测定和有些其他研究中,可以用铁盐溶液代替抗血清。例如,在OP1型噬菌体的一步生长曲线实验中,用铁盐或抗血清处理所得的结果是非常接近的。

The serological slide agglutination test method has long been used only to identify the diseases caused by Pseudomonas solanacearum and Erwinia salicis since it was deveped by Gehrnig in 1962 and Morton in 1965 for the identification of the former diseases and by Dickinson and Lucas in 1977 for the identification of the latter ones.The method was first successully applied to identify the diseases caused by Xanthomonas oryzae & Erwinla carotovora var Carotovora besides Ps.solanacearum in the present study. The...

The serological slide agglutination test method has long been used only to identify the diseases caused by Pseudomonas solanacearum and Erwinia salicis since it was deveped by Gehrnig in 1962 and Morton in 1965 for the identification of the former diseases and by Dickinson and Lucas in 1977 for the identification of the latter ones.The method was first successully applied to identify the diseases caused by Xanthomonas oryzae & Erwinla carotovora var Carotovora besides Ps.solanacearum in the present study. The plant sap of the disease portion was used for the test.The serological reaction completed within 10-20 minutes.Negative result was obtained whenever the specific antiserum was used to test against the sap of them non corresponding diseases plant.The method is simple but accurate and is considered to be very useful for a rapid identification of most bacterial diseases.

血清学玻片凝集反应方法自从1962年Gehring和1965年Morton以及1977年Dickinson和Lucas分别展开了对青枯病(pseudomonas solanacearum)和柳树萎蔫病(Erwinia Salicis)鉴定以来,直到目前为止还只应用于这些病的鉴定。 本报告介绍的方法除了能用于青枯病鉴定之外还第一次成功地应用于水稻白叶枯病(Xanthomonas oryzae)和白菜软腐病(Erwinia Carotovora Var.carotovora)的鉴定。以植物病原细菌抗血清与其相对应的病株汁液进行玻片凝集反应试验,在10—20分钟内可以见到明显的凝集反应现象,而与非对应的病株汁液进行试验则没有反应,方法简单而准确,对于这些细菌病的快速鉴定是非常有用的。 玻片凝集反应试验虽然已在某些植物病毒病的田间鉴定中广泛应用,但在植物细菌病害方面的应用不多。早在六十年代,Morton、Gehring等用青枯菌抗血清与其相对应的病害汁液进行玻片凝集反应试验获得成功。但此法应用很少,发展很慢,直至七十年代除作物青枯病(Ps.Solanacearum)外还只有柳树萎蔫病(Erwinia Salicis)...

血清学玻片凝集反应方法自从1962年Gehring和1965年Morton以及1977年Dickinson和Lucas分别展开了对青枯病(pseudomonas solanacearum)和柳树萎蔫病(Erwinia Salicis)鉴定以来,直到目前为止还只应用于这些病的鉴定。 本报告介绍的方法除了能用于青枯病鉴定之外还第一次成功地应用于水稻白叶枯病(Xanthomonas oryzae)和白菜软腐病(Erwinia Carotovora Var.carotovora)的鉴定。以植物病原细菌抗血清与其相对应的病株汁液进行玻片凝集反应试验,在10—20分钟内可以见到明显的凝集反应现象,而与非对应的病株汁液进行试验则没有反应,方法简单而准确,对于这些细菌病的快速鉴定是非常有用的。 玻片凝集反应试验虽然已在某些植物病毒病的田间鉴定中广泛应用,但在植物细菌病害方面的应用不多。早在六十年代,Morton、Gehring等用青枯菌抗血清与其相对应的病害汁液进行玻片凝集反应试验获得成功。但此法应用很少,发展很慢,直至七十年代除作物青枯病(Ps.Solanacearum)外还只有柳树萎蔫病(Erwinia Salicis)应用来进行快速鉴定。 由于细菌性病害的常规鉴定方法比较复杂,工作量较大,需要较长时间才能完成,既不能满足检疫工作的需要,也很不容易用来确诊在实验过程中出现的一些不典型症状的标样以及人工接种发“病”的大批?

 
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