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carboxylesterase
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  carboxylesterase
The results showed that, feeding on clone 7, the activity of esterase, carboxylesterase, and mixed-function oxidases in the midgut of the larvae was very much decreased.
      
The obtained data suggest that the synergistic effect on the common cockroaches and houseflies is chiefly due to carboxylesterase inhibition by monothioderivatives and monooxygenase suppression by dithioderivatives, respectively.
      
Organophosphate insecticide (parathion/diazinon) resistance in housefly (Musca domestica L.) is associated with the change in carboxylesterase activity.
      
In addition, we checked the malathion carboxylesterase (MCE), percent remaining activities in acetylcholinesterase (AChE), glutathion-S-transferase (GST), and general esterase activities in all five strains used in this study.
      
Organophosphate (OP) insecticides (parathion/diazinon) resistance in housefly (Musca domestica L.) is associated with the change in carboxylesterase activity.
      
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In South China the Lychee stink bog is a very serious pest of the Lychee tree.It passes only one generation each year.It was found that there is a great variation in the tolerance (natural resistance) of the adults to trichlorfon in the spring and winter.In order to study the mechanism of tolerance,experiments were conducted to monitoring the enzyme activity and observation were made on the ultrast-ructures of the fat bodies and the effects of trichlorfon in various periods of 1985-1386 in Guangzhou. Results...

In South China the Lychee stink bog is a very serious pest of the Lychee tree.It passes only one generation each year.It was found that there is a great variation in the tolerance (natural resistance) of the adults to trichlorfon in the spring and winter.In order to study the mechanism of tolerance,experiments were conducted to monitoring the enzyme activity and observation were made on the ultrast-ructures of the fat bodies and the effects of trichlorfon in various periods of 1985-1386 in Guangzhou. Results of studies showed that the enzyme activity of the alkaline phosphatase was high in the newly-emerged adults:the activity being 1.83 μmol/min,during the overwintering period,the activity was the lowest, being 0.30μmol/min and during the reproductive period being 0.67μmoi/min. After treatment with trichlorfon by topical application, theactivity was increased ,indicating the effect of induction. The activity of acid phosphatase was found to be different from that of the alkline phosphatase. From the newly-emerged adults passing through the winter to the reproductive period in the spring, the enzyme activity decreased gradually from 5.7μoml/min to 5.47μmol/min, 4.8μmol/min, respectively. There was a marked decreased after treatment with trichlorfon, particularly in October and December. The carboxylesterase was found to be similar to that of alkaline phosphatase, for the newly-emerged adults, 1.48mmol/min, overwintering, 0.42mmol/min and reproductive period,0.72mmol/min.Trichlorfon also induced the activity of the enzyme.Upon examination with electronic microscope,many vacuoles were observed in the fat bodies of the newly-emerged adult ,however,during the overwintering period granules of glycogen were found,and during the reproductive period linear,rough endoplasmic reticulum network was found to be dominant.No significant change in the ultrastructure of the fat bodies was found in the adults treated with trichlorfon,indicating that the target site of action might not be in the fat bodies.Spraying of trichlorfon twice a year in the spring and early summer when the tolerance of the insect to insecticides is the lowest has been used as a very effective method of controlling the bug for over 20 years in South China and up to the present no resistance to trichlorfon has ever been observed. This,serves as a good example that sophisticated application of insecticides may avoid or delay occurence of pesticide resistance in insect populations in the field and deserves further investegations.

本文研究了荔枝蝽象(Tessarotoma papillosa Drury)在不同季节期间脂肪体酯酶活力及其超微结构的特点,并探讨了敌百虫对它们的影响。结果表明:1.碱性 磷酸酯酶在新羽化成虫中活力较高,为1.83微克分子/分钟,到越冬期最低,仅0.30微克分子/分钟,在生殖期活力为0.67微克分子/分钟。用敌百虫处理后对酶活力有诱导作用。2.酸性 磷酸酯酶的变化与碱性磷酸酯酶不同,从新羽化成虫经越冬到生殖期,酶活力从高逐渐降低,分别为5.7微克分子/分钟,5.47微克分子/分钟和4.8微克分子/分钟。敌百虫处理后其酶活力有所降低。3.羧酸酯酶的活力变化与碱性磷酸酯酶相似,新羽化成虫为1.48毫克分子/分钟,越冬虫为0.42毫克分子/分钟,生殖期为0.72毫克分子/分钟。敌百虫对此酶也有诱导作用。4.新羽化成虫的脂肪体亚细胞结构以液泡为主,越冬期以脂肪、精原颗粒为多,到生殖期线粒体、粗面内质网大量出现。未观察到敌百虫处理后的生理病变。本文还讨论了有关自然抗药性机理的一些问题。

Detection and monitoring for resistance of Lipaphis erysimi pseudobrassicae to OPs by bioassays and examination of esterases that are associated with resistance levels in single adult aptere aphids was carried out. The biochemical tests estimated the resistant individuals in two field populations in Shanghai. The two colonies collected from Jinshan and Meilong were compared for resistance to OP insecticides and their esterase activities.The results obtained by bioassay indicated that Jinshan colony had some...

Detection and monitoring for resistance of Lipaphis erysimi pseudobrassicae to OPs by bioassays and examination of esterases that are associated with resistance levels in single adult aptere aphids was carried out. The biochemical tests estimated the resistant individuals in two field populations in Shanghai. The two colonies collected from Jinshan and Meilong were compared for resistance to OP insecticides and their esterase activities.The results obtained by bioassay indicated that Jinshan colony had some resistance to dimethoate (5.7×), fenitrothion (4.0×)and malathion(1.8×) compared with Meilong colony. It showed that resistance of the aphids to OPs was relative to some increase in esterase activity.Estimating the Carboxylesterase (CarE) activity in single aphids by esterase determination provided a rapid and accurate method for detecting resistance and for estimating the proportion of OP-resistant aphids. The CarE activity in single aphids fell into three groups: low (OD< 0.3), moderate (OD: 0.3-0.5) and high (OD>0.6). The aphids with low CarE activity were 8% for Jinshan and 30% for Meilong. These with moderate activity were 85% (Jinshan) and 68% (Meilong). The aphids with high activity were 7 % and 2 % respectively.

以生物测定及羧酸酯酶活性测定的方法,检测了上海地区菜缢管蚜对有机磷的抗药性。测定结果表明,金山群体对乐果、杀螟松和马拉硫磷的抗药性分别是梅陇的5.7、4.0和1.8倍。金山群体的羧酸酯酶活性高于梅陇。说明其抗性与羧酸酯酶活性增高有关。单个蚜虫的羧酸酯酶活性测定是检测抗性及抗性个体在群体内分布的一种既迅速又精确的方法。如把蚜虫的羧酸酯酶活性分成3类:低羧酸酯酶活性(OD<0.3)、中等羧酸酯酶活性(OD:0.3—0.5)和高羧酸酯酶活性(OD>0.6),则金山与梅陇种群低羧酸酯酶活性个体分别占8%、30%;中等活性个体分别占85%、68%;高活性个体分别占7%、2%。

The insensitivity to decamethrin and change of hydrolase activity of Aphis gossypii Glover in different regions and stages were examined by applying bioassay and biochemical methods. The results indicated that the aphid in Xinxiang area is highly insensitive to decamethrin. In the same area, it is more insensitive in square and boll stage than in seedling stage. In vitro, the carboxylesterase activity increases along with the intensified aphid insensitivity, while there is no significent change in phosphatase....

The insensitivity to decamethrin and change of hydrolase activity of Aphis gossypii Glover in different regions and stages were examined by applying bioassay and biochemical methods. The results indicated that the aphid in Xinxiang area is highly insensitive to decamethrin. In the same area, it is more insensitive in square and boll stage than in seedling stage. In vitro, the carboxylesterase activity increases along with the intensified aphid insensitivity, while there is no significent change in phosphatase.

本文采用生物测定和生物化学方法就棉蚜(Aphis gossypii Glover)在不同地区不同时期对溴氰菊酯不敏感性与水解酶的变化进行了初步研究。结果表明:新乡地区棉蚜对溴氰菊酯存在高倍不敏感性。同一地区,蕾铃期棉蚜不敏感性高于苗期棉蚜。在离体条件下,棉蚜羧酸酯酶的活力随着棉蚜对溴氰菊酯不敏感性增加而增加。磷酸酯酶活性变化则不显著。

 
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