4. 28 bands of soluble protein appeared in seedlings of salt tolerant variants of wheat, whereas only 26 bands in CK. 98 kD,75 kD,52 kD,49 kD and 32 kD bands were specific in salt tolerant line. 38 kD,35 kD and 21 kD protein bands only appeared in CK.
(3)Three additional bands of gliadin(B2,B3,B4) were found in the grains of salt tolerant wheat line and B1 band content increased. However B5 band of the control was markedly stronger than that in salt tolerant line;
and 4.28 bands of soluble protein appeared in seedling of salt tolerant variant, whereas only 26 bands in CK. 98 kD,75 kD,52 kD,49 kD and 32 kD bands were specific in salt tolerant line, 38 kD,35 kD and 21 kD protein bands only appeared in the control. Characters of salt tolerance in wheat were discussed.
3.Three additional bands of gliadin(b2,b3,b4)were found in the grains of salt tolerant wheat line, while the content of b1 was greater. However, b5 band of CK was markedly stronger than that in salt tolerant line;
Thefree proline content in NaCl-tolerant cell line grownon 1.0% NaClcontaining medium remarkably increased,and was 3.6 and 2.7times higherin alfalfa and-sainfoin salt-tolerant line respectively than that in controlcell line.
Segments of immature inflorescences and immature embryos of the salt-tolerant wheat (850512r) were used as explants for secondary selection of a new salt-tolerant cell line and selection of mannitol-tolerant cell line.
The results of SDS electrophoresis of soluble proteins indicated that the amount of scanning bands of proteins in NaCl-tolerant cell line was more than that in control. New protein bands of 59kd and 90kd were observed in salt-tolerant cell line.
However, no significant difference of isoeasterase patterns between salt - tolerant cell line and the control cell line was observed though the total enzyme activity in tolerant cell line was lower than the control's.
When grown on NaCl-frce medium, the relative osmotic potential of tolerant cell line was lower than that of control cell line, however, free proline content and the activity of superoxide dismutase (SOD) were 105% and 90% higher than those in controls.
However, no significant difference of isoeasterase patterns between salt - tolerant cell line and the control cell line was observed though the total enzyme activity in tolerant cell line was lower than the control's.
The putative salt tolerant line was superior in saline soil for all parameters measured, indicating that the mechanism selected in cells in vitro was also active in whole plants, and that the trait is genetically stable and seed transmitted.
Fifty-two-day old plants of a salt tolerant line, S24 and a salt sensitive, Yecora Rojo were subjected for 15 days to 125 mol·m-3 NaCl in Hoagland's nutrient solution under glass-house conditions.
The dry matter of shoots and roots of the salt tolerant line was significantly greater over all time intervals in saline substrate than the salt sensitive line, Yecora Rojo.
The salt tolerant line Dunkeld had significantly higher fresh and dry masses of shoots, and seed yield than salt sensitive line Cyclon in all salinities.
Two sensitive breeding lines BR4608-R1-R2 and BR4909-R1-R2 were crossed with a salt tolerant line IR13146-13-3-3 to transfer its salt tolerant character to the doubled haploids.
The other putative salt-tolerant line (PST-1) had a higher background level of proline that may have enabled it to survive salt stress during initial screening, although its later performance was no better than the control plants.
A NaCl-tolerant cell line of eggplant has been isolated, as a spontaneous variant, on agar solidified medium supplemented with 1% NaCl (Electrical conductivity -17.5 m mho/cm), a concentration lethal to the wild type cells.
Calli were induced from segments of hypocotyledon in Medicago sativaL.and Onbrychis viciaefolia.1.0%NaC1—tolerant cell line was selectedby increasing the concentration of NaCl in the medium gradually.Thefree proline content in NaCl-tolerant cell line grownon 1.0% NaClcontaining medium remarkably increased,and was 3.6 and 2.7times higherin alfalfa and-sainfoin salt-tolerant line respectively than that in controlcell line.The activity of peroxidase in NaCl-tolerant cell line was higherthan that of control lin...
NaCl-tolerant lines of 'Jincheng' and 'Taoyecheng' (Citrus sinensis) were obtained by subjecting their nucellar calli to γ ray and EMS, and 10 generations of selection in vitro. Later tests confirmed that their tolerance was stable. The tolerant lines accumulated more proline, maintained higher level of K~+ , and absorbed less Cl~- and Na~+ than the original calli. The protoplasts from the tolerant lines developed embryoids after 2 months culture, and they germinated into plantlets. Besides, it was found th...
A stable NaCl-tolerant cell line was obtained by treating the embryogenic calli ini-tiated from young inflorescence segments of millet (Setaria italica, Var.7520) with ethyl methane sulphonate (EMS) after screening for 8 months and stabilizing for about 6 months. The regenerated plants were produced from it. Physiological and biochemical analysis showed that, the NaCl-tolerant cell line grown on the NaCl-free medium for 3 generations exhibited stronger resistance to NaCl stress and water stress caused by PE...
耐盐系
salt tolerant line
查询“耐盐系”译词为其他词的双语例句
查询“耐盐系”译词为用户自定义的双语例句
相关查询:
2008 CNKI－中国知网