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| | Spleen cells from the C3H/HeN mice immunized by vaccinia virus antigen were fused with myelome cells of SP 2/0. Ten hybridoma cell strains were set up,whieh were capable cf secreting the monoclonal antibedy against the vaccinia virus. They were all well purified, highly specific, active in the hemagglubunatien inhibition reaction and classific, as IgG2a or IgG3 in terms of Ig classification, In this paper, the distribution of virus-antigen in the cells and the cell surface infected b... | | 本文用痘苗病毒作抗原免疫C3H/HeN系小鼠,取脾与SP2/0骨髓瘤细胞融合,建立了10株分泌抗痘苗病毒McAb的杂交瘤细胞株。还用McAb对痘苗病毒感染细胞表面和细胞浆内的病毒抗原分布进行了测定。 | | 文摘来源 | | The existance of blood-epididymis barrier has been confirmed. The Ig classification of some monoclonal human sperm and mouse sperm antibodies (Mabs) were determined by immunodiffusion. The mature male mice were passively immunized with these Mabs, HS11, HS63, MS204, MS207 (IgG_1) and MS79, MS212 (IgM) by intraperitoneal injections. Sera and sperm were recovered from the immunized mice and assayed by immunofluorescenceo It was found that all Mabs could enter the blood circulation but ... | | 用琼脂糖双扩散法测得抗人精子单克隆抗体HS11、HS63和抗小鼠精子单抗MS204,MS207属IgG_1,抗小鼠精子单抗MS79、MS212属IgM。将上述单抗通过腹腔注射被动免疫正常CD-1雄性小鼠,然后收集受鼠血清及其睾丸、附睾各段的精子作免疫荧光染色。发现所有抗精子单抗均能进入血循环,但被血-睾屏障所阻挡。HS11、HS63、MS204和MS207能进入附睾管腔,与相应的精子抗原发生免疫反应,但染色百分率较低。MS79、MS212与附睾各段精子均无染色反应。提示血-附睾屏障能阻止IgM,但不排斥IgG_1。 | | 文摘来源 | | Anti-DNA antibodies were purified from the sera of patients with Systemic Lupus Erythematosus (SLE) by a new affinity chromatographic cloumn (DNAT). Enzyme-linked immunosorbent assay (ELISA) results of anti-DNA antibodies showed that anti-DNA antibody is high heterogeneous with respect to its binding ability to DNA, the composition of anti-ssDNA is not only more complexed than that of anti-dsDNA, but binding energy of ssDNA/anti-ssDNA is also greater than that of dsDNA/anti-dsDNA. Although the IgG ant... | | 本文用国产高分子树脂(T)接枝小牛胸腺DNA,通过亲合层析从系统性红斑狼疮SLE患者血清中纯化出抗-ds DNA抗体和抗-ss DNA抗体。酶联免疫吸附分析(ELISA)的研究表明:SLE抗-DNA抗体和DNA结合的差异性很大,是高度非均一性的。抗-ss DNA抗体不仅组成成分比抗-ds DNA抗体复杂,ss DNA/抗-ssDNA亲合能力也明显高于ds DNA/抗-ds DNA。纯化的抗-DNA抗体以IgG类抗体占主导,同时也有其它类型抗体存在(例如IgM等)。抗-ds DNA抗体有较抗-ss DNA抗体高的IgG含量(两者的IgG/IgM分别是7.0和4.0),说明IgG抗-DNA抗体更倾向于同dsDNA结合。 | | 文摘来源 | |   |
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