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inducing cysts
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  相似匹配句对
     Inducing expression.
     3.表达蛋白鉴定
短句来源
     Cysts of serendipity
     偶然发现的囊肿
短句来源
     inducing time, 3 h;
     诱导时间 3h ; 培养基起始 pH 6 .5 ;
短句来源
     Fetal Ovarian Cysts
     胎兒卵巢囊腫
短句来源
     Influence of Inducing Conditions and Specific Diapause Deactivation Methods on Hatchability of Two Species Artemia Cysts Produced in Lab
     两种卤虫休眠卵的终止滞育及休眠卵的诱导发生
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The culture of Scrippsiella trochothea was established from germinated cyst isolated from Dapeng Bay (southem China) sediment samples in January 1993.The clonal strain STDP 01 was re-isolated from a single cell of this culture. The morphology of vegetahve cells and cysts of Scrippsiella trochoidea were observed using a scanning electronic microscope. The vegetative cell shows no obvious plates,which is different from other Peridnales species. The cyst spherical to ovoid, has a diameter from...

The culture of Scrippsiella trochothea was established from germinated cyst isolated from Dapeng Bay (southem China) sediment samples in January 1993.The clonal strain STDP 01 was re-isolated from a single cell of this culture. The morphology of vegetahve cells and cysts of Scrippsiella trochoidea were observed using a scanning electronic microscope. The vegetative cell shows no obvious plates,which is different from other Peridnales species. The cyst spherical to ovoid, has a diameter from 15 to 30μm. The processes are calcareous and vary in length and in shape (from blunt to pointed). A theropylic archeropyle was observed. The different stages of life cycle were observed under the control of light, temperatllre and nutrient under lab condihons. The life cycle belongs to a typical dinoflagellate cycle which include a vegetahve cycle (cell division), a temporary cyst cycle and a reshng cyst cycle. Reshng cyst cycle formahon could be fulfilled by sexual reproduction and was formed from planozygote by fusion of two isogametes. In terms of the condihons for cyst formaton and germinahon, when the nutrients were plenty, the vegetahve cells of Scrippsiella trochoidea grew well and could reach a maximum growth ratio of 0.42d-1.Mitrate depletion was a key factor to induce cyst formation. When the vegetative cells were grown in the f/ 40 (25μmol/L NH4+, no added NO3-) medium, the first cyst aPPeared at the stationery stage or the end of log phase (8th days) and cyst formation ratio reached 8.7%. Phosphate depletion can reduce the growth rate of vegetahve cells but cannot induce cyst formaton or affect the cyst formation ratio. Newly formed cysts can germinate only after a Period of dormancy (3 months) and when nitrogen is repleted and light and temperature are suitable. Chfferent temperatures (8, 15, 20, 25℃)and light intensihes (200, 600, 1 600, 2 800, 4 500, 6 000 and 7 500lx) were adopted to test the germination raho of cysts after being stored in dark refrigerator in 4-5℃for three months. The results reveal that temperature has a positive relation with germination among 8, 15 and 20℃. The germinahon ratio can reach a maximum of 90% at 200℃, 30% at 15℃ and 10% at 8℃. The germinahon ratio was 80% at 25℃,which was less than 20℃. Even weak light intensity (200lx) can initiate cyst germinaton. After replehon with a fresh f/ 2 medium for tWo weeks, cyst germination raho in different light intensihes (600-7500lx) could reach the same (80%-90%). high light intensity (7 500lx) causes a decrease in the germinahon time,but has little effect on the germination ratio. The results also show that cyst formation and rapid vegetative reproduction have a close relationship with nitrate nutrient.Eutrophicahon is a key factor for bloom outbreak.

锥状斯氏藻STDP01克隆品系于1993年1月分离自大鹏湾底泥中的单个锥状斯氏藻孢囊,并经单细胞分离培养成藻株。在电子显微镜下,对该藻的营养细胞和孢囊形态进行了观察,该藻营养细胞与多甲藻目的其它甲藻不同,没有非常明显的甲板;孢囊球形或卵形,直径15—30μm,突起钙化长短大小不一,从钝到尖。萌发孔为中间(theropylic)型。在实验室光、温及营养盐的人工调控下,运用倒置显微镜,对锥状斯氏藻生活史进行了追踪,发现该藻属典型的甲藻生活史,由营养生长、暂时性休眠和休眠孢囊三个阶段组成。孢囊形成通过有性生殖,经两配于结合形成浮游合于并进一步形成泡囊。对孢囊形成和萌发的条件进行探讨的结果表明,在营养盐充足的条件下锥状斯氏藻生长良好,最大比生长率可达0.42d-1;氮盐缺乏是诱寻其孢囊形成的关键因子,当锥状斯氏藻生长在f/2缺氮(以25μmol/LNH4+代替NO3-)条件下孢囊形成率可达87%;磷酸盐缺乏虽然使营养生长减缓,但并不能诱导孢囊形成;对孢囊形成率也无显著影响。新形成的孢囊必须在经过一段时间休眠后,在氮源充足,光照、温度合适时方可萌发。孢囊在冰箱中经三个月储存后,在不同的温度(8,15,20和25℃)和光...

锥状斯氏藻STDP01克隆品系于1993年1月分离自大鹏湾底泥中的单个锥状斯氏藻孢囊,并经单细胞分离培养成藻株。在电子显微镜下,对该藻的营养细胞和孢囊形态进行了观察,该藻营养细胞与多甲藻目的其它甲藻不同,没有非常明显的甲板;孢囊球形或卵形,直径15—30μm,突起钙化长短大小不一,从钝到尖。萌发孔为中间(theropylic)型。在实验室光、温及营养盐的人工调控下,运用倒置显微镜,对锥状斯氏藻生活史进行了追踪,发现该藻属典型的甲藻生活史,由营养生长、暂时性休眠和休眠孢囊三个阶段组成。孢囊形成通过有性生殖,经两配于结合形成浮游合于并进一步形成泡囊。对孢囊形成和萌发的条件进行探讨的结果表明,在营养盐充足的条件下锥状斯氏藻生长良好,最大比生长率可达0.42d-1;氮盐缺乏是诱寻其孢囊形成的关键因子,当锥状斯氏藻生长在f/2缺氮(以25μmol/LNH4+代替NO3-)条件下孢囊形成率可达87%;磷酸盐缺乏虽然使营养生长减缓,但并不能诱导孢囊形成;对孢囊形成率也无显著影响。新形成的孢囊必须在经过一段时间休眠后,在氮源充足,光照、温度合适时方可萌发。孢囊在冰箱中经三个月储存后,在不同的温度(8,15,20和25℃)和光照(200,600160028004500,6000和7500lx)下测试其萌发情

To evaluate four different methods to induce encystation of Giardia lamblia in vitro,induction of the encystation of Giardia lamblia C2 strain was carried out using the four different ways in vitro and then the numbers of induced cysts were calculated.The result showed that these four methods all could induce encystation of Giardia lamblia C2 strain in vitro,and the numbers of induced cysts were different.The third method is the most efficient one to induce cysts in vitro could...

To evaluate four different methods to induce encystation of Giardia lamblia in vitro,induction of the encystation of Giardia lamblia C2 strain was carried out using the four different ways in vitro and then the numbers of induced cysts were calculated.The result showed that these four methods all could induce encystation of Giardia lamblia C2 strain in vitro,and the numbers of induced cysts were different.The third method is the most efficient one to induce cysts in vitro could the number of cysts can reach 1×105/mL.In conclusion,the third method is a convenient and efficient way to induce encystation of Giardia lamblia C2 strain in vitro.

评价 4种不同的体外诱导蓝氏贾第鞭毛虫成囊方法。采用 4种已报道的方法对蓝氏贾第鞭毛虫C2株进行体外诱导成囊 ,并统计包囊数目。结果表明 4种方法都可诱导成囊 ,但成囊数之间存在显著差异。方法 3获得的包囊数最高 ,为 1× 10 5/mL。方法 3为体外诱导蓝氏贾第鞭毛虫C2株成囊的一种简便且易获得大量包囊的方法。

 
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