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cmv-cp
相关语句
  cmv-cp
     Plant expression vector pE14 with double resistances to virus and insect has obtained by insert-ing CMV-cp gene and Bt-toxin gene into the T-DNA of a same Agrobacterium tumefaciens(A.t)binilry vec-tor pE3 one by one,Tobacco was transformed with A.
     通过将抗病的CMV-cp基因和抗虫的Bt-toxin基因分别加上GaMV35S启动子和终止子,依次插入到同一植物表达载体pE3的T-DNA上,获得双抗载体pE14。
短句来源
     t0.GV311-SE containing pE14.The Nopaline assay andthe PCR amplification of CMV-cp gene and Bt-toxin gene from regenerated plants indicate that CMV-cp geneand Bt-toxin gene have been introduced into tobacco genome by T-DNA of pE3.Virus and insect bioasssaysshow that some transgenic plants can resistance the infecthn of CMV and the damage of Manduca sexta.
     然后载体pE14以土壤农杆菌GV311-SE介导转化烟草,再生植株的胭脂碱检测及PCR扩增证明CMV-CP基因和Bt-toxin基因已随T-DNA同时导入烟草植株中。 抗病抗虫实验表明转基因植株具有较强的抗病毒CMV的能力和抗烟青虫的能力。
短句来源
     The results showed that the Ikb CMV-CP gene did integrate with the genome of the regenerated plant.
     结果表明:再生植株基因组中整合了CMV-CP基因,长度为1kb,并能有效表达。
短句来源
  cmv—cp
     Using Agrobacterium rhizogenes with Ri-plsmid (PRiA4b) to be medium,the binary vector PBTC 8 (T-DNA with CMV-cp and TMV-cp genes) was transforred in to cultivated tomato of Heilongiiang province.
     本文用含PRi(PRiA4b)的发根农扦菌为介导,将二元载体质粒PBTC-8(T—DNA上有TMV—CP和CMV—CP基因),导入黑龙江省当地番茄品种。
短句来源
     After test by Luis method,some anti-kan plants showed the activity of nopalin synthase. Further tests, such as: PCR, dot blot,western blot, etc,indicated that the CMV-CP gene had integrated in the genomic DNA of the anti-kan plants, and the expression of this 1 kb gene in plant produced a protein of 24 kDa, an expected size for CMV-CP. The expression amount was about 5% of total plant protein.
     进一步经SDS-PAGE,DOT-Elisa,PCR特异扩增,Western-Blot等方法检测,结呆证明了Km抗性植株基因组中整合了CMV—CP基因,长度为1kb,并能有效表达.表达产物分子量为24kD,确为CMV的外壳蛋白,其表达量占细胞总蛋白的5%。
短句来源
  “cmv-cp”译为未确定词的双语例句
     Agrobacterium-mediated transformation of pepper 'B162'with coat protein gene of cucumber mosaic virus(CMV-CP) was studied.
     以辣椒品种‘B162’为试材,探讨了通过根癌农杆菌介导法将黄瓜花叶病毒外壳蛋白(CMV-CP)基因转化辣椒的适宜条件.
短句来源
     Inheritance of kanamycin-resistance and CMV-resistance in transgenic chili pepper expressing CMV-CP and TMV-CP genes
     CP基因转化的线辣椒抗卡那霉素和抗CMV特性的遗传
短句来源
     Resistance to Cucumber Mosaic Virus (CMV) in the Transgenic Tomatoes Inserted by Ribozyme and Antisense, Sense Genes of CMV-CP
     转核酶基因、反义和正义CMV-CP基因番茄的抗黄瓜花叶病毒(CMV)研究
短句来源
     PCR amplification confirmed that the CMV-CP gene has been integrated into the genome of the Passiflora plantlets in some plantlets.
     通过对所获得的再生植株进行PCR扩增检测 ,结果表明CMV -Pf -CP基因已导入西番莲植株中。
短句来源
     In order to express coat protein (CP) of CMV correctly, a pair of primers were designed in accordance with the reported CMV-CP gene sequences. CP gene of CMV was amplified from the recombinant plasmid PBIC carrying CP gene by polymerase chain reaction(PCR). It was cloned into the prokaryotic expression vector pET-22b(+).
     根据已报道的CMV-CP基因序列,设计一对引物,从含CMV-CP蛋白基因的重组质粒pBIC中,通过PCR扩增得到大小为657nt的CMV-CP基因,并把它重组到细菌表达载体pET-22b(+)中。
短句来源
更多       
  相似匹配句对
     Cp.
     Cp.
短句来源
     ConclusionGenciclovir is effect and safety for CMV infection of CP children.
     结论更昔络韦治疗脑瘫患儿CMV感染安全有效。
短句来源
     CMV_(cp) Gene Transformation and Expression in Pepper
     黄瓜花叶病毒壳蛋白基因转化辣椒及其在转基因株后代的表达
短句来源
     However, CP.
     而CP.
短句来源
     Study on Introducing Anti-CMV Virus CP Gene into Cucumber
     抗CMV病毒外壳蛋白CP基因导入黄瓜的研究
短句来源
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  cmv-cp
The genes CMV-cp and ToLCV-cons-rep from the isolates of cucumber mosaic virus (CMV) and tomato leaf curl virus (ToLCV) were transcriptionally fused under the control of CaMV 35 S promotor.
      
Plants expressing the transgenic proteins were then analysed by PCR using CMV-CP gene-specific primers.
      
Kanamycin sprayed on seedling leaves and DAS-ELISA were used to detect NPTII and CMV-CP, respectively.
      
Western blot analysis of CMV-CP containing R1 plants showed that two of them accumulated significant levels of the foreign gene product while the other two expressed it only, to low levels.
      
Southern analysis of DNA isolated from putative transgenic plants revealed that 3 out of 5 R1 plant lines reacted positively with the CMV-CP gene.
      
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The pure intaot chloroplasts were isolated from protoplasts releasedfrom tobacco leaves infected by Cucumber Mosaic Virus(CMV),or from thetransgenic tobacco leaves expressing CMV coat protein(CP).The CP wasdetected by Western Blotting using the antiserum agaist CMV CP subunit.We concluded that:1,CP was found inside chloroplasts of CMV infectedtobacco;2.The concentration of CP inside chloroplasts was positively rela-ted to the severity of the symptom;3.CP was not associated with the chl-oroplasts from the transgenic...

The pure intaot chloroplasts were isolated from protoplasts releasedfrom tobacco leaves infected by Cucumber Mosaic Virus(CMV),or from thetransgenic tobacco leaves expressing CMV coat protein(CP).The CP wasdetected by Western Blotting using the antiserum agaist CMV CP subunit.We concluded that:1,CP was found inside chloroplasts of CMV infectedtobacco;2.The concentration of CP inside chloroplasts was positively rela-ted to the severity of the symptom;3.CP was not associated with the chl-oroplasts from the transgenic tobacco.It was suggested that the presence ofCP inside chloroplasts is related to the Mosaic symptom on tobacco.

由感染黄瓜花叶病毒(CMV)、健康和转外壳蛋白(CP)基因的烟叶以原生质体法制备纯化的叶绿体。经SDS—PAGE电泳,银染色,比较其蛋白质图谱。用CMV外壳蛋白游离亚基制备的抗血清进行Western blotting。结果发现:1.CP存在于CMV侵染的烟叶绿体中。2.叶绿体中CP的浓度和花叶症状严重程度呈正相关;3.表达CP的转基因烟草叶绿体中未测出CP存在。根据以上结果,认为CMV侵染的烟花叶症状产生与CP进入叶绿体有直接相关性。

The intact chloroplasts were made through protoplasts. In the SDSPAGE-silver stained gel, one extra protein band, which had the same mobility and molecular weight as the cucumber mosaic virus coat protein (CMV-cp), was found in the diseased chloroplast comparative to the health chloroplast. In the immunoblot testing, the band was highly reactive to the antiserum against the free CMV-cp, while the healthy and diseased sap treated healthy chloroplasts had no reaction. The result provides an evidence...

The intact chloroplasts were made through protoplasts. In the SDSPAGE-silver stained gel, one extra protein band, which had the same mobility and molecular weight as the cucumber mosaic virus coat protein (CMV-cp), was found in the diseased chloroplast comparative to the health chloroplast. In the immunoblot testing, the band was highly reactive to the antiserum against the free CMV-cp, while the healthy and diseased sap treated healthy chloroplasts had no reaction. The result provides an evidence for the CMV-cp existence in the chloroplast. It was also found that the concentration of CMV-cp inside chloroplast was positively related to the severity of the symptom.

用原生质体法制备出高纯度的完整叶绿体经SDS-PAGE电泳,银染后,发现黄瓜花叶病毒(CMV)侵染的烟草病叶叶绿体蛋白质图谱和健叶叶绿体相比,多出一条染色较弱的迁移率与CMV衣壳蛋白质相同的带,经Western转移,用CMV游离衣壳蛋白亚基抗血清进行斑点酶联(Immunoblot)检测,证明这条带就是CMV衣壳蛋白质。健康叶绿体中加入去掉叶绿体的病叶汁液而制备出的叶绿体中无CMV衣壳蛋白质,说明这不是在叶绿体提纯过程中得到的假象,即衣壳蛋白质存在于被CMV侵染的完整叶片叶绿体中。这个结果否认了以往报道的CMV衣壳蛋白质不存在于烟草叶绿体中的结论。另外还发现,叶绿体中的衣壳蛋白质浓度与叶片症状严重程度呈正相关。

Using Agrobacterium rhizogenes with Ri-plsmid (PRiA4b) to be medium,the binary vector PBTC 8 (T-DNA with CMV-cp and TMV-cp genes) was transforred in to cultivated tomato of Heilongiiang province. The method of hypocotyl,apical half of epicotyl and cutting cotyledon sections was used to induct hairy roots. Agropion and mannopine were detected in half of these hairy roots by electrophores. Some hairy roots were get by the method of sdlection medium with kanomycine resistance. The adventitious bud and regeneration...

Using Agrobacterium rhizogenes with Ri-plsmid (PRiA4b) to be medium,the binary vector PBTC 8 (T-DNA with CMV-cp and TMV-cp genes) was transforred in to cultivated tomato of Heilongiiang province. The method of hypocotyl,apical half of epicotyl and cutting cotyledon sections was used to induct hairy roots. Agropion and mannopine were detected in half of these hairy roots by electrophores. Some hairy roots were get by the method of sdlection medium with kanomycine resistance. The adventitious bud and regeneration plant were induced from cullus with kanomycine resistance on infected cotyledon piece. Detecting the alkaloid, PCR and DIBA (Dot Immunobinding Assy),it certified there were TMV-cp and CMV-cp genes, Agropion and mannopin in the regeneration plant We got deuble traslative plant.

本文用含PRi(PRiA4b)的发根农扦菌为介导,将二元载体质粒PBTC-8(T—DNA上有TMV—CP和CMV—CP基因),导入黑龙江省当地番茄品种。用胚轴注射,顶端切口涂抹,子叶切块穿刺等方法感染转化子菌液,诱导出毛状根。滤纸电泳检测有50%以上的毛状根含有农抒诚和甘露碱。用抗性筛选法亦获得有卡那霉素抗性的毛状根。在穿刺感染子叶切块诱导产生的具卡那霉素抗性的愈伤组织上,获得不定芽与再生植株,经冠瘿碱,PCR,斑点免疫结合法检测证明再生植株中有TMV和CMV基因产物的两种外壳蛋白和农扦碱与甘露碱,获得双转化植株。

 
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