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p蛋白
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  p protein
    Comparison of the deduced amino acid sequence of the RYSV gene 2-encoded protein with those of VSV P protein,RV M1 protein and SYNV M 2 pro ̄tein,which all encoded by genes at the same location in the respective viral genomes,revealed no extensive sequence homologies. Nevertheless,a peptied stretch of about 30 amino acids displaying significant sequence similarity with the above mentionde rhabdovirus proteins could be iden ̄tified.
    RYSV基因2编码的蛋白质的氨基酸序列与其它弹状病毒基因2编码的磷酸化蛋白(VSV的P蛋白,RV的M1蛋白和SYNV的M2蛋白)相比,缺乏广泛的同源性,但尚存在一个长约30个氨基酸的序列保守区域。
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  p protein
The possible mechanisms of the formation of the modified ADH-P protein are discussed in connection with the differential activity of genes in the microgametophytes of angiosperms.
      
The immunoblot analysis with RSV-infected and non-infected HEp-2 cells as antigen revealed the expected age-dependent low prevalence of G protein antibodies and clear seroconversion of N and P protein antibodies.
      
Are anti-ribosomal P protein antibodies relevant in systemic lupus erythematosus
      
Cellular expression pattern of the glycine decarboxylase P protein in leaves of an intergeneric hybrid between the C3-C4 interme
      
The densities of NF150D and NF150P protein spots on the Coomassie blue-stained two-dimensional gels of the normal and injured cords also displayed alterations similar to the immunocytochemical data.
      
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The nucleotide sequence of the gene adjacent to the nucleocapsid protein gene on the rice yellow stunt virus (RYSV) genome,tentatively named gene 2,has been determined from cDNA clones isolated from a RYSV genomic cDNA library.The 5′ end sequence of the gene 2 was putatively assigned as 5′-AACAC-3′, based on homologies to the 5′ end sequences of other RYSV genes and the consensus transcription initiation sequences of other rhabdoviruses.The 3′ end of the gene 2 was defined by sequence analysis of the cDNA corresponding...

The nucleotide sequence of the gene adjacent to the nucleocapsid protein gene on the rice yellow stunt virus (RYSV) genome,tentatively named gene 2,has been determined from cDNA clones isolated from a RYSV genomic cDNA library.The 5′ end sequence of the gene 2 was putatively assigned as 5′-AACAC-3′, based on homologies to the 5′ end sequences of other RYSV genes and the consensus transcription initiation sequences of other rhabdoviruses.The 3′ end of the gene 2 was defined by sequence analysis of the cDNA corresponding to the 3′ region of the gene 2 transcripts existed in RYSV-infected rice plants.lt was thus concluded that the RYSV gene 2 was 1211 nucleotides (nt) long,comprising a 42nt untranslated 5′region followed by a 969nt open reading frame(including the termination codon)and a 200nt untranslated 3′ sequence.Comparison of the deduced amino acid sequence of the RYSV gene 2-encoded protein with those of VSV P protein,RV M1 protein and SYNV M 2 pro ̄tein,which all encoded by genes at the same location in the respective viral genomes,revealed no extensive sequence homologies.Nevertheless,a peptied stretch of about 30 amino acids displaying significant sequence similarity with the above mentionde rhabdovirus proteins could be iden ̄tified.Moreover the RYSV gene 2 encoded protein contained seven potential Casein kinase Ⅱ phosphorylation sites (S/T-X-X-D/E),suggesting it might be also a phosphoprotein.We then postulate that the RYSV gene 2 might encode the NS protein.

从水稻黄矮病毒(RYSV)基因组的cDNA文库中,获得了包含邻接核衣壳蛋白基因的基因2的克隆,并测定了其核苷酸序列。RYSV基因2的5′端起始序列推测为5′-AACAC-3′,该起始序列与RYSV其他基因及其他弹状病毒基因的5′端起始序列高度同源。通过测定感病水稻体内RYSV基因2的转录物的3′端序列,精确地定位了RYSV基因2的3′端终止位点。由此确定RYSV基因2全长1211个核苷酸,它含有42个核苷酸的5′端非翻译区,969个核苷酸的蛋白质编码区(包括一个终止密码子)及200个核苷酸的3′端非翻译区。RYSV基因2编码的蛋白质的氨基酸序列与其它弹状病毒基因2编码的磷酸化蛋白(VSV的P蛋白,RV的M1蛋白和SYNV的M2蛋白)相比,缺乏广泛的同源性,但尚存在一个长约30个氨基酸的序列保守区域。而且RYSV基因2编码的蛋白中含有7个可被CaseinkinaseⅡ磷酸化的位点(S/T-X-X-D/E),有可能它也是一个磷酸化蛋白。因此推测RYSV基因2编码RYSVNS蛋白。

 
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