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   p73蛋白 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.098秒
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p蛋白
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  “p73蛋白”译为未确定词的双语例句
    Pathological Examination of the Chick Embryo Inoculated by NDV F_48E_9 Strain and Cloning,Expression of P Gene of NDV F_48E_9 Strain
    新城疫病毒F_48E_9株接种鸡胚的病理学观察及其P蛋白基因的克隆与表达
短句来源
    The result indicated that ND_(98)、ND_(99) strains had the same structural proteins as Lasota and F_(48)E_8 strains.
    SDS-PAGE结果显示,ND98、ND99与F48E8、Lasota具有相似的结构蛋白:HN蛋白、F蛋白、NP蛋白P蛋白、M蛋白;
短句来源
    The expression of the reporter gene depends on the delicate ratio of the viral N and P proteins in a plasmid-based reverse genetic system .
    同时本研究也证明在质粒为基础的反向遗传系统中,由RNA聚合酶I表达质粒产生的小的转录本也具有功能性,本研究再确证报告基因的表达依赖于N和P蛋白的比率。 本研究为研究鲍纳病病毒的3’和5‘末端非编码区功能和挽救感染性鲍纳病病毒奠定了基础。
短句来源
    It was found that the Phas 87% and 91% homologies with La sota in nudeotide and amine acidelevel ,respectively.
    通过序列分析发现F_(48)E_9株P蛋白基因核苷酸序列与La Sota株的P蛋白基因核苷酸同一性为87%,氨基酸同源性为91%,表明F_(48)E_9株与La Sota株有一定亲缘关系。
短句来源
    The infected insect cells were collected at different timepost-infection, analysed by SDS-PAGE. The results showed that the expressedproduct had a molecular mass of approximately 56kD.
    再将重组病毒感染对数生长期的sf9昆虫细胞,于感染后24、48、72、96小时收获感染细胞,经SDS-PAGE电泳分析,证明NDV F_(48)E_9株P蛋白基因在重组杆状病毒系统中获得了表达,其分子量大小约为56kD。
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  p protein
The possible mechanisms of the formation of the modified ADH-P protein are discussed in connection with the differential activity of genes in the microgametophytes of angiosperms.
      
The immunoblot analysis with RSV-infected and non-infected HEp-2 cells as antigen revealed the expected age-dependent low prevalence of G protein antibodies and clear seroconversion of N and P protein antibodies.
      
Are anti-ribosomal P protein antibodies relevant in systemic lupus erythematosus
      
Cellular expression pattern of the glycine decarboxylase P protein in leaves of an intergeneric hybrid between the C3-C4 interme
      
The densities of NF150D and NF150P protein spots on the Coomassie blue-stained two-dimensional gels of the normal and injured cords also displayed alterations similar to the immunocytochemical data.
      
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  p protein
The possible mechanisms of the formation of the modified ADH-P protein are discussed in connection with the differential activity of genes in the microgametophytes of angiosperms.
      
The immunoblot analysis with RSV-infected and non-infected HEp-2 cells as antigen revealed the expected age-dependent low prevalence of G protein antibodies and clear seroconversion of N and P protein antibodies.
      
Are anti-ribosomal P protein antibodies relevant in systemic lupus erythematosus
      
Cellular expression pattern of the glycine decarboxylase P protein in leaves of an intergeneric hybrid between the C3-C4 interme
      
The densities of NF150D and NF150P protein spots on the Coomassie blue-stained two-dimensional gels of the normal and injured cords also displayed alterations similar to the immunocytochemical data.
      
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  phosphoprotein
The CD4 complex included the following major components: tyrosine phosphatase CD45, transferrin receptor CD71, tyrosine kinase Lck, and a lymphocyte phosphatase-associated phosphoprotein LPAP.
      
The differences revealed could not be accounted for by the interference of ATPases or phosphoprotein phosphatases.
      
We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media.
      
We suggest that phosphoprotein kinase Pho85p is involved in either phosphorylation of a highly specific proline permease, Put4p, or in signaling proline concentration.
      
Disruption of the PHO85 gene encoding phosphoprotein kinase (Pho4p is the substrate of this enzyme) leads to alleviation of glucose repression of the CIT1 gene.
      
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M13 PⅢ was used as a vector, and the study on acquired B cell epitope for Chlamydia psittaci recongnized by McAb17 was carried out.Recombinant plasmid containing gene of B cell epitope was constructed by using reconstructed prokaryotic expression vector pQE30.The results of ELISA and Western blot demonstrated that McAb17 specifically reacted against the expressed protein.The serum antibody of a rabit that was immunized by the expressed protein can be used for detection of Chlamydia psittaci antigen.

利用噬菌体PⅢ蛋白为载体对鹦鹉热衣原体单抗 17筛选得到的B细胞抗原表位进行了研究。利用改构后的原核表达载体pQE3 0 ,构建含有B细胞表位基因的重组表达质粒 ,表达的蛋白经ELISA及WesternBlot实验证实能够与单抗C17发生特异反应 ,蛋白免疫动物后得到了抗鹦鹉热衣原体的抗体 ,验证了所筛选表位的真实性

Two newcastle disease viruses(NDV) isolated from chickens and ostriches were designated as ND_(98) and ND_(99) respectively.ND_(99) was lentogenic strain and ND_(98) was mesogenic strain.The structural proteins of ND_(98)、ND_(99)、F_(48)E_8、Lasota stains were analyzed by SDS-PAGE and Western-Blotting.The result indicated that ND_(98)、ND_(99) strains had the same structural proteins as Lasota and F_(48)E_8 strains.Western-Blotting test suggested that antigen of ND_(98) and ND_(99) existed some difference,ND_(98)...

Two newcastle disease viruses(NDV) isolated from chickens and ostriches were designated as ND_(98) and ND_(99) respectively.ND_(99) was lentogenic strain and ND_(98) was mesogenic strain.The structural proteins of ND_(98)、ND_(99)、F_(48)E_8、Lasota stains were analyzed by SDS-PAGE and Western-Blotting.The result indicated that ND_(98)、ND_(99) strains had the same structural proteins as Lasota and F_(48)E_8 strains.Western-Blotting test suggested that antigen of ND_(98) and ND_(99) existed some difference,ND_(98) and F_(48)E_8 strains shared the same immunogen as ND_(99) and Lasota strains.

2株新城疫病毒ND98、ND99分别分离自发病鸡群与雏鸵鸟,ND98属于中发型毒株,ND99属于缓发型毒株。将2株分离株ND98、ND99与参考毒株F48E8、Lasota进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、蛋白质转移印迹(Western-Blotting)。SDS-PAGE结果显示,ND98、ND99与F48E8、Lasota具有相似的结构蛋白:HN蛋白、F蛋白、NP蛋白与P蛋白、M蛋白;Western-Blotting试验显示,ND98与F48E8、ND99与Lasota具有相似的免疫原,ND98、ND99免疫原性存在一定的差异。

Seventy serum samples were collected from adult brown ducks in Hubei were collected in order to clarify the natural Duck hepatitis B virus (DHBV) infection in and to characterize the genome structure of DHBV. The complete genome of the DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the natural infection rate of DHBV in Hubei was 10%. The DHBV genome isolated by PCR (GenBank accession number DQ276978 ) had 3 024 nucleotides with three...

Seventy serum samples were collected from adult brown ducks in Hubei were collected in order to clarify the natural Duck hepatitis B virus (DHBV) infection in and to characterize the genome structure of DHBV. The complete genome of the DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the natural infection rate of DHBV in Hubei was 10%. The DHBV genome isolated by PCR (GenBank accession number DQ276978 ) had 3 024 nucleotides with three overlapping reading frames encoding the surface, core and the polymerase proteins, respectively. Comparison of genome sequences of this strain with those of 17 DHBV strains from the GenBank revealed an identity from 89.3% to 93.5% at the nucleotide level. The amino acid sequences of the S protein,core protein,and functional domain of the Pol protein were highly reserved among all of these DHBV strains. This Hubei strain was found to share more signature amino acids in the polymerase genes with the “Chinese” DHBV strains than those of the “Western” country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, this Hubei DHBV strain should be classified to a subtype of the Chinese strains.

为了解湖北地区Thisfindingwasalsoconfirmedbythephylogenetictreeanalysis.麻鸭中鸭乙型肝炎病毒(DuckhepatitisBvirus,DHBV)自然感染状况以及湖北麻鸭所携带DHBV的基因结构特征,采集了70份成年麻鸭血清并应用PCR技术检测DHBVDNA,对其中一份DHBVDNA阳性血清进行DHBV全基因扩增,并进行克隆与序列测定分析。结果表明,湖北麻鸭DHBV自然携带率为10%;湖北DHBV分离株(GenBank登录号DQ276978)基因组的全长为3024bp,有编码P,S和C蛋白的三个开放阅读框;与GenBank中17株DHBV基因组比较,核苷酸同源性介于89.85%~93.29%之间;S蛋白、C蛋白和P蛋白结构功能区序列均高度保守;而对P蛋白标志性氨基酸和全基因进化树的分析表明,该分离株属于DHBV中国基因型中的一个亚型。

 
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