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   p73蛋白 在 基础医学 分类中 的翻译结果: 查询用时:0.078秒
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  p protein
    The P protein coded by HBV Pol has 3 functional domains and 1 spacer (SP) that are lined up for terminal protein (TP), SP, reverse transcriptase/polymerase (RT/PR) and RNase H. Up to now, the HBV Pol and its functional domains have not been clearly clarified.
    HBV Pol基因在ORF中最长,并且与C、S、X基因区有重叠,其编码的P蛋白含有3个功能域和1个无意义的隔离片(spacer,SP),排列顺序为N-末端蛋白(TP),SP,逆转录酶(RT)/DNA聚合酶(PR)和核糖核酸酶H(RNase H)。
短句来源
    Methods The RNA polymerase of influenza virus was isolated from virus particles by stepwise centrifugation in different salts. The 3P proteins were analyzed by SDS-PAGE and Western-blotting using monospecific antibodies against each P protein. The vRNA was detected using urea-denatured PAGE.
    方法 用甘油、CsCl1及CsTFA不同介质的密度梯度超速离心法进行分离 ,用SDS -PAGE电泳及Westernblot法检测 3P蛋白 ,用尿素变性PAGE检测vRNA。
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  “p73蛋白”译为未确定词的双语例句
    The four RV P gene nucleotide homology was 83.6%-99.8%,the amino acid homology was 87.2%-99%; the sequence interacting! with LC8 was located on 143-148 amino acids remnant bases and was DKSTQT.
    四株病毒P基因核苷酸和氨基酸序列同源性分别为83.6%~99.8%和87.2%~99%,P蛋白与胞浆动力蛋白轻链LC8相互作用的序列位于143~148位氨基酸残基,均为DKSTQT,四株病毒P基因与L蛋白、N蛋白作用位点序列显示未发生影响其生物学功能的变异。
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    Although BNIPL has a SEC14 domain, the deletion of sec14 has not been complemented by BNIPL in Saccharomyces cerevisiae.
    虽然BNIPL含有酿酒酵母Sec14p的SEC14 domain,但是基因功能互补实验结果发现,在酵母细胞内BNIPL并不能与酿酒酵母Sec14p蛋白功能互补。
短句来源
    A DNA fragment, which encoded the heat stable protein kinase inhibitor (PKI) (5-24) of cAMP dependent protein kinase(cAPK), was synthesized and cloned into phage display vector fd tet DOG1. Thus, PKI(5-24) was displayed on the surface of phage fd, which was termed PKI phage (cAPK inhibitor phage), in a form fused with gene III protein (g3p).
    人工合成编码cAMP依赖蛋白激酶(cAPK)的热稳定抑制剂第5~24位氨基酸[PKI(5~24)]的DNA片段,并将之克隆到phagedisplay载体fd-tet-DOG1中,使PKI(5~24)以融合于g3p蛋白的形式展示于噬菌体表面,从而得到PKI噬菌体。
短句来源
    Objective To establish the method for isolation and purification of the RNA polymerase PB1, PB2 and PA from the influenza virus in order to investigate the structure and the function of the polymerase.
    目的 建立分离、纯化流感病毒RNA聚合酶PB1、PB2和PA 3P蛋白的有效方法 ,为进一步研究 3P蛋白的结构和功能奠定基础。
短句来源
    The P-RNA (P proteins-viral RNA) complexes were further dissociated into 3P proteins and viral RNA by cesium trifluoroacetate (CsTFA) gradient centrifugation.
    进一步将 3P -RNA在CsTFA -甘油双组分不连续密度梯度介质中进行超速离心 ,可有效地将 3P与RNA分离 ,获得较纯的 3P蛋白
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A DNA fragment, which encoded the heat stable protein kinase inhibitor (PKI) (5-24) of cAMP dependent protein kinase(cAPK), was synthesized and cloned into phage display vector fd tet DOG1. Thus, PKI(5-24) was displayed on the surface of phage fd, which was termed PKI phage (cAPK inhibitor phage), in a form fused with gene III protein (g3p). It was showed that PKI phage not only repressed cAPK effectively, but also bound with the immobilized recombinant His 6 tag mouse cAPK Cα(His 6 mCα) specifically....

A DNA fragment, which encoded the heat stable protein kinase inhibitor (PKI) (5-24) of cAMP dependent protein kinase(cAPK), was synthesized and cloned into phage display vector fd tet DOG1. Thus, PKI(5-24) was displayed on the surface of phage fd, which was termed PKI phage (cAPK inhibitor phage), in a form fused with gene III protein (g3p). It was showed that PKI phage not only repressed cAPK effectively, but also bound with the immobilized recombinant His 6 tag mouse cAPK Cα(His 6 mCα) specifically. The bound PKI phages could be quantitatively eluted under acidic conditions. Model affinity screening demonstrated that PKI phages could be selectively enriched from the mixture of PKI phages and wild type phages (1∶10 8) using affinity chromatography of immobilized His 6 mCα. These results suggest that selecting protein kinase inhibitor by phage display technique is feasible.

人工合成编码cAMP依赖蛋白激酶(cAPK)的热稳定抑制剂第5~24位氨基酸[PKI(5~24)]的DNA片段,并将之克隆到phagedisplay载体fd-tet-DOG1中,使PKI(5~24)以融合于g3p蛋白的形式展示于噬菌体表面,从而得到PKI噬菌体。蛋白激酶抑制剂活性测定表明PKI噬菌体可有效地抑制cAPK的活性。结合实验结果显示PKI噬菌体能与固相化小鼠cAPK催化亚基α(His6-mCα)高亲和结合,并在酸性条件下被定量洗脱。模型亲和筛选实验结果表明,利用固相化His6-mCα亲和层析,可以从108倍的野生型噬菌体背景中选择性富集专一PKI噬菌体。本工作为构建用于蛋白激酶结构功能等研究的特殊PhageDisplay随机肽库成功地探索了可行途径。

The synthesized random oligonucleotides which encode nonapeptides are cloned into the filamentous bacteriophage vector fUSE5,and transformed into the competent E.coli MC1061 by multielectroporation,10 10 clones are obtained in which each peptide expressed as a fusion to the minor coat protein pⅢ and displayed on the surface of the phage.Affinity selection has been used to identify potent ligands for the human type Ⅳ collagenase and a family of collagenase binding bacteriophage ligands was obtained.ELISA...

The synthesized random oligonucleotides which encode nonapeptides are cloned into the filamentous bacteriophage vector fUSE5,and transformed into the competent E.coli MC1061 by multielectroporation,10 10 clones are obtained in which each peptide expressed as a fusion to the minor coat protein pⅢ and displayed on the surface of the phage.Affinity selection has been used to identify potent ligands for the human type Ⅳ collagenase and a family of collagenase binding bacteriophage ligands was obtained.ELISA results showed 20 positive clones that could bind the type Ⅳ collagenase specifically.Sequences showed two relatively short conserved subsequences:WDXXD and WVGXXR,the sequence WDXXD is homologous to the ScFv variable region of the type Ⅳ collagenase.It is showed that the peptide library is a powerful tool for the selection of the potent peptide ligands for proteins.The construction and screening of the nonapeptide library forms the bases for the further application.

将人工合成的编码九肽的随机序列DNA片段克隆进丝状噬菌体表达载体fUSE5,经多次电击转化和表达,获得肽段与噬菌体pⅢ蛋白融合并展示在噬菌体表面的随机序列九肽表位肽库。库容量达1010个克隆。以Ⅳ型胶原酶为靶蛋白,采用亲和纯化筛选模式,从中筛选出Ⅳ型胶原酶结合肽。进一步ELISA检测筛选出与Ⅳ型胶原酶特异结合的20个阳性克隆。序列分析发现一组肽含有WDXXD的共同序列,一组含有WVGXXR的共同序列。其中WDXXD的序列与Ⅳ型胶原酶单链抗体可变区序列同源。结果表明,多肽库是筛选蛋白特异结合肽的有力工具,表位九肽库的构建和筛选方法的建立为进一步应用筛选具有高亲和力的特异结合肽奠定了基础。

Objective To get information about phosphoprotein (P) genes of RSV field strains isolated in China. Methods The complete nucleotide sequences of the P protein genes from six respiratory syncytial virus strains were determined with cDNA clones which had been amplified by RT PCR and cloned into PTZ18R plasmid vector and compared with those of previously published counterparts of A2 and CH18537 strains. Results The data indicated that there had high identities (>95%) at nucleotide and amino acid levels among...

Objective To get information about phosphoprotein (P) genes of RSV field strains isolated in China. Methods The complete nucleotide sequences of the P protein genes from six respiratory syncytial virus strains were determined with cDNA clones which had been amplified by RT PCR and cloned into PTZ18R plasmid vector and compared with those of previously published counterparts of A2 and CH18537 strains. Results The data indicated that there had high identities (>95%) at nucleotide and amino acid levels among the strains within the same subgroup; there were more divergence among strains (<85%) from different subgroups at nucleotide and amino acid levels of P genes,especially in the 3′ noncoding regions and the divergent domains. The analysis of hydrophobility indicated that there had difference from 50 to 100 amino acid of P proteins between A and B subtypes. Conclusion The analysis of the P genes confirm that there existed variability among RSV field strains and strains A2 and CH18537. The data described above revealed the characteristics of the P genes of RSV field strains isolated in China.

目的 了解我国呼吸道合胞病毒 (RSV)地方株的P(Phosphoprotein ,P)蛋白基因状况和变异特征。方法 选取不同地区具有不同流行特征的 6株RSV地方株 ,以提取的病毒mRNA为模板进行逆转录 聚合酶链扩增 (RT PCR)扩增、目的基因的克隆及序列测定分析。结果 地方株和同亚型原型株之间P蛋白核苷酸序列及推导出的氨基酸序列同源性均超过 95 % ;不同亚型间存在较大差异 ,核苷酸全序列同源性低于 85 % ,尤其在 3′端非编码区及中间变异区。由氨基酸序列推导出的疏水性分析图显示 ,A、B亚型中间变异区存在疏水性的不同 ,这为解释A、B亚型间P蛋白电泳迁移率的不同提供了一种可能。结论 我国RSV地方株与原型株之间的P蛋白无论在核苷酸水平还是在氨基酸水平均存在一定的变异。这些结果对于了解我国RSV地方株的基因状况提供了有益的资料

 
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